期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 15, 期 2, 页码 -出版社
SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3382908
关键词
medical imaging; multiphoton processes; nonlinear optics; nonlinear image processing; scanning microscopy; tissues
资金
- Natural Sciences and Engineering Research Council of Canada
- Ontario Centre of Excellence for Photonics
- Canada Foundation for Innovation
- Ontario Innovation Trust
Imaging hematoxylin-and-eosin-stained cancerous histological sections with multicontrast nonlinear excitation fluorescence, second- and third-harmonic generation (THG) microscopy reveals cellular structures with extremely high image contrast. Absorption and fluorescence spectroscopy together with second hyperpolarizability measurements of the dyes shows that strong THG appears due to neutral hemalum aggregation and is subsequently enhanced by interaction with eosin. Additionally, fluorescence lifetime imaging microscopy reveals eosin fluorescence quenching by hemalums, showing better suitability of only eosin staining for fluorescence microscopy. Multicontrast nonlinear microscopy has the potential to differentiate between cancerous and healthy tissue at a single cell level. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3382908]
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