Article
Biochemical Research Methods
Imane Lboukili, Georgios Stamatas, Xavier Descombes
Summary: Reflectance confocal microscopy (RCM) allows for real-time visualization of the skin structure at the cellular level. In this study, an automated pipeline based on membrane detection was designed to identify and analyze epidermal cells in RCM images. The proposed method achieved higher accuracy compared to machine learning-based approaches.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Biochemical Research Methods
Zhi Lu, Yu Liu, Manchang Jin, Xin Luo, Huanjing Yue, Zian Wang, Siqing Zuo, Yunmin Zeng, Jiaqi Fan, Yanwei Pang, Jiamin Wu, Jingyu Yang, Qionghai Dai
Summary: Virtual-scanning light-field microscopy (VsLFM) uses a physics-based deep learning model to improve the quality and speed of LFM, reducing motion artifacts and enabling challenging demonstrations such as fast 3D voltage imaging in Drosophila. By constructing a 40 GB high-resolution scanning LFM dataset across different species, VsLFM exploits physical priors and bypasses hardware scanning to achieve ultrafast 3D imaging in various processes such as the beating heart in embryonic zebrafish and neutrophil migration in the mouse liver.
Article
Multidisciplinary Sciences
Arianna Bresci, Jeong Hee Kim, Silvia Ghislanzoni, Francesco Manetti, Lintong Wu, Federico Vernuccio, Chiara Ceconello, Salvatore Sorrentino, Ishan Barman, Italia Bongarzone, Giulio Cerullo, Renzo Vanna, Dario Polli
Summary: Screening for anticancer therapy in vitro can lead to the identification of additional treatments and improve clinical outcomes. This study demonstrates the potential of all-optical, label-free, and quantitative microscopy techniques in early detection of therapy-induced senescent cells in tumor cells, providing a rapid and accurate method for research in anticancer treatment.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Review
Oncology
Stefania Guida, Antonio Alma, Kaleci Shaniko, Johanna Chester, Silvana Ciardo, Ilaria Proietti, Roberta Giuffrida, Iris Zalaudek, Marco Manfredini, Caterina Longo, Francesca Farnetani, Giovanni Pellacani
Summary: Non-melanoma skin cancers (NMSC) can be monitored using noninvasive skin imaging in nonsurgical treatments. The accuracy of dermoscopy, reflectance confocal microscopy (RCM), and optical coherence tomography (OCT) in detecting clearance is uncertain. We aim to report the proportion of persisting tumors identified with noninvasive technologies available in the literature.
Article
Multidisciplinary Sciences
Stephan Allgeier, Andreas Bartschat, Sebastian Bohn, Rudolf F. Guthoff, Veit Hagenmeyer, Lukas Kornelius, Ralf Mikut, Klaus-Martin Reichert, Karsten Sperlich, Nadine Stache, Oliver Stachs, Bernd Koehler
Summary: This article describes a novel approach for real-time creation and visualization of a mosaic image of the SNP, allowing for evaluation of the acquired image data quality during the examination. Online mosaicking also offers the chance to identify overlap with previous images, which is particularly advantageous for follow-up examinations.
SCIENTIFIC REPORTS
(2022)
Review
Multidisciplinary Sciences
Colin J. R. Sheppard
Summary: Structured illumination microscopy and image scanning microscopy are two rapidly growing microscopic techniques that can enhance transverse spatial resolution and/or improve axial imaging performance. This article reviews the history and principles of these techniques and compares the imaging properties of the two methods.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2021)
Article
Biology
Raidel Herrera-Pereda, Alberto Taboada Crispi, Danilo Babin, Wilfried Philips
Summary: A novel algorithm for segmenting cells of the cornea endothelium layer on confocal microscope images is proposed, achieving higher accuracy compared to existing segmentation methods. The algorithm includes steps such as applying a difference of Gaussian filter, automatic thresholding, and fitting inscribed circles into cell regions for final segmentation. Parameters of interest are computed and compared against existing methods, showing good agreement with state of the art techniques.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Letter
Oncology
Shrikant B. Mali
Summary: Intravital microscopy and optical coherency tomography are powerful imaging tools that have revolutionized cancer diagnosis and therapies. They allow for real-time observation of biological processes in vivo, providing insights into tumor behaviors. Recent developments in techniques for observing deep tissues have improved the detection of subcellular events and made these technologies indispensable in basic sciences and drug development.
Article
Orthopedics
Linjun Yang, Marc J. Brouillette, Mitchell C. Coleman, Paige N. Kluz, Jessica E. Goetz
Summary: This study developed an automated framework for analyzing chondrocytes in 3D image stacks of cartilage, providing fast and accurate quantification of cell density and intracellular activities. The framework uses a seeded watershed-based algorithm to segment cells, eliminating duplicate segmentation and automatically defining tissue volume.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Review
Immunology
Anika Gruneboom, Oliver Aust, Zuelal Cibir, Flora Weber, Dirk M. Hermann, Matthias Gunzer
Summary: Innate immune cells play a crucial role in defense against infections and sterile inflammation, with recent advances in microscopy providing insights into their biology. Neutrophils and macrophages have protective roles during tumor growth and ischemia-reperfusion damage, but can also exacerbate diseases. Future directions include enhancing knowledge about innate immune cells and their potential applications in disease diagnostics.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Computer Science, Interdisciplinary Applications
Liang Han, Hang Su, Zhaozheng Yin
Summary: Phase contrast microscopy is a noninvasive imaging technique that can monitor the behavior of transparent cells without staining or altering them. However, the imaging images of phase contrast microscopy contain artifacts that hinder cell segmentation and detection. In this research, we accurately formulated the imaging model of phase contrast microscopy and proposed an image restoration procedure using a deep neural network, which enables high quality cell segmentation.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2023)
Article
Multidisciplinary Sciences
Chia-Wei Hsu, Chun-Yu Lin, Yvonne Yuling Hu, Shean-Jen Chen
Summary: A dual-resonant scanning multiphoton (DRSM) microscope with a tunable acoustic gradient index of refraction lens and a resonant mirror enables high-speed volumetric imaging. By sampling the multiphoton excited fluorescence signal at a rate of one pixel per laser pulse, a frame rate of around 8000 Hz in the x-z plane and a volumetric imaging rate of over 30 Hz for a large image volume of 256 x 256 x 80 voxels are achieved.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
Nikolaos M. Dimitriou, Salvador Flores-Torres, Joseph Matthew Kinsella, Georgios D. Mitsis
Summary: The invasion of cancer cells into surrounding tissues is a key feature of cancer, but a precise quantitative understanding of their spatiotemporal patterns is still lacking. 3D cell cultures offer a more realistic model for studying these patterns compared to traditional 2D cultures. This study presents an image processing and segmentation pipeline for detecting 3D GFP-fluorescent triple-negative breast cancer cell nuclei, and analyzes their spatial patterns and temporal evolution.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Chemistry, Applied
Diego Navarro-Barreda, Begona Bedrina, Cesar A. A. Angulo-Pachon, Juan F. F. Miravet, Dolores Perez-Sala, Francisco Galindo
Summary: Four new BODIPY derivatives were synthesized and characterized, with a focus on their effects on cellular uptake, intracellular location, and fluorescence imaging abilities. The results showed that one of the new molecules displayed superior lysosomal location compared to the others.
Article
Biochemical Research Methods
Steven L. Jacques, Stephane Roussel, Ravikant Samatham
JOURNAL OF BIOMEDICAL OPTICS
(2016)
Article
Biochemical Research Methods
Faezeh Talebi Liasi, Ravikant Samatham, Steven L. Jacques
JOURNAL OF BIOMEDICAL OPTICS
(2017)
Article
Biochemical Research Methods
Ravikant Samatham, Steven L. Jacques, Paul Campagnola
JOURNAL OF BIOMEDICAL OPTICS
(2008)
Article
Biochemical Research Methods
Steven L. Jacques, Ravikant Samatham, Niloy Choudhury
BIOMEDICAL OPTICS EXPRESS
(2010)
Article
Biochemical Research Methods
Steven L. Jacques, Bo Wang, Ravikant Samatham
BIOMEDICAL OPTICS EXPRESS
(2012)
Article
Optics
Ravikant Samatham, Kevin G. Phillips, Steven L. Jacques
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2010)
Review
Dermatology
Tracy Petrie, Ravikant Samatham, Alexander M. Witkowski, Andre Esteva, Sancy A. Leachman
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2019)
Article
Dermatology
Tracy Petrie, Ravikant Samatham, Shaun M. Goodyear, Dan E. Webster, Sancy A. Leachman
SEMINARS IN CUTANEOUS MEDICINE AND SURGERY
(2019)
Article
Biochemistry & Molecular Biology
Qiuchen Guo, Duanchen Sun, Alexander S. Barrett, Sonali Jindal, Nathan D. Pennock, Matthew W. Conklin, Zheng Xia, Elizabeth Mitchell, Ravikant Samatham, Naomi Mirza, Steven Jacques, Sheila Weinmann, Virginia F. Borges, Kirk C. Hansen, Pepper J. Schedin
Summary: Mammographically-detected breast density has an impact on breast cancer risk and progression, and the presence of fibrillar collagen plays a crucial role in breast density. This study investigated the physiological factors influencing collagen production in the breast. Through the analysis of gene expression in female rats, the study revealed a triphasic pattern of collagen gene regulation and reproductive state-dependent composition. The findings were confirmed in human breast tissue from premenopausal women. The study also found a correlation between the collagen gene signature and poor progression-free survival in breast cancer patients. These findings contribute to a better understanding of normal breast function, the etiology of breast density, and breast cancer risk and outcomes.
Proceedings Paper
Optics
Ravikant Samatham, Niloy Choudhury, Alfons L. Krol, Steven L. Jacques
PHOTONIC THERAPEUTICS AND DIAGNOSTICS VIII, PTS 1 AND 2
(2012)
Article
Instruments & Instrumentation
R. Samatham, I-S Park, K. J. Kim, J-D Nam, N. Whisman, J. Adams
SMART MATERIALS AND STRUCTURES
(2006)
Article
Engineering, Chemical
Ravikant Samatham, Kwang J. Kim
POLYMER ENGINEERING AND SCIENCE
(2006)