期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 13, 期 2, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.2904667
关键词
heart rate; differential interference contrast; imaging; correlation
To develop an accurate and convenient method for measuring the heart rate of zebra fish in vivo, a system combining fast differential interference contrast (DIC) imaging with an autocorrelation technique is established. The imaging correlation coefficient corr(i,j) between frame i, selected from the obtained time-lapse imaging series as the reference image, and any other frame j, is calculated as the time-dependent cycle course. Heat rate is determined by the cycle period of the corr with a high temporal resolution of 4 ms, achieved by fast charge-coupled device (CCD) imaging of 250 frames per second. With this high-resolution system, we find that 1-mg/L cadmium not only induces the slowing of the heart rate, but also caused signs of arrhythmia in treated fish. (C) 2008 Society of Photo-Optical Instrumentation Engineers.
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