期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 107, 期 5, 页码 1551-1559出版社
WILEY
DOI: 10.1002/jbm.b.34247
关键词
bioprosthetic heart valves; 3; 4-hydroxyphenylpropionic acid; horseradish peroxidase and hydrogen peroxide; elastin stabilization; calcification
资金
- National Key Research and Development Programs [2016YFC11022002017YFC1104200, 2016YFC1102200, 2017YFC1104200]
- Program of Introducing Talents of Discipline to Universities (111 Project) [B16033]
- Fundamental Research Funds for the Central Universities [YJ201641]
- Young Elite Scientists Sponsorship Program by CAST [2017QNRC001]
- National Natural Science Foundation of China [31700833]
The failures of glutaraldehyde (GLUT) cross-linked bioprosthetic heart valves (BHVs) are mainly due to degeneration and calcification. In this study, we developed a new preparation strategy for BHVs named as HPA/EDC/EGCG that utilized 3,4-hydroxyphenylpropionic acid (HPA)-conjugated pericardium, epigallocatechin gallate (EGCG), and horseradish peroxidase (HRP)/hydrogen peroxide (H2O2) enzymatic cross-linking. HPA-pericardium conjugation was done by carbodiimide coupling reaction using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Then HPA-conjugated pericardium was cross-linked by HRP/H2O2 enzyme-catalyzed oxidation. The feeding ratios of HPA and EGCG were optimized. The consumption of amino groups, collagenase and elastase degradation in vitro, biomechanics, extracellular matrix stability, and calcification of HPA-/EDC-/EGCG-treated pericardiums were characterized. We demonstrated that HPA-/EDC-/EGCG-treated pericardiums had better elastin stabilization and less calcification. EGCG and enzymatic cross-linking treated pericardiums showed improved mechanical properties. This new EGCG and enzymatic cross-linking strategy would be a promising method to make BHVs with better elastin stability and anti-calcification property. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1551-1559, 2019.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据