期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 100B, 期 8, 页码 2307-2315出版社
WILEY
DOI: 10.1002/jbm.b.32802
关键词
silk fibroin scaffold; gelatin; chitooligosaccharide; glutaraldehyde; conjugation; osteogenic differentiation
资金
- Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission [AS615A]
- Chulalongkorn University Centenary Academic Development Project
In this study, the surface modification of silk fibroin (SF) scaffolds with gelatin/chitooligosaccharide (G/COS) blends using the reaction of glutaraldehyde (GA) was established. The effects of G/COS mixing ratio (100/0, 90/10, 80/20, and 70/30) and GA crosslinking concentration (0.05, 0.10, 0.15, and 0.20 vol %) on the properties of scaffolds were investigated. At 0.100.20 vol % GA, all G/COS blends could be successfully conjugated on the SF scaffolds, as confirmed by the percentage of weight increased and the presence of functional groups indicating SF, G, and COS from FTIR spectra. Pore size of SF scaffolds was around 570 mu m with 92% porosity, however, the G/COS-conjugated SF scaffolds showed thickened pore's wall, smaller pore size (similar to 184275 mu m) and less porosity (similar to 81%), but increased density. This modified structure subsequently improved the compressive modulus of the G/COS-conjugated SF scaffolds. In terms of biological properties, the gelatin-conjugated SF scaffolds promoted the attachment and proliferation of bone marrow-derived mesenchymal stem cells (MSC) rather than the other scaffolds. However, the G/COS-conjugated SF scaffolds, particularly at the ratio of 70/30, promoted the osteogenic differentiation of MSC comparing to the SF scaffold, as confirmed by the production of alkaline phosphatase (ALP) activity and calcium (Ca), and the deposition of calcium phosphate (CaP). It was concluded that the G/COS-conjugated SF scaffolds showed great mechanical properties due to the beta-structure of silk fibroin, as well as the enhanced biological properties due to the G/COS blends. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
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