Article
Dentistry, Oral Surgery & Medicine
Danyal A. Siddiqui, Alikhan B. Fidai, Smriti G. Natarajan, Danieli C. Rodrigues
Summary: This study developed an oral bacterial biofilm model that simulates the temporal shifts of the oral microbiome during plaque formation and maturation. The results showed that oral bacterial adhesion was similar on surface-treated dental implant materials.
Review
Chemistry, Analytical
Agnieszka Nawrocka, Ireneusz Piwonski, Salvatore Sauro, Annalisa Porcelli, Louis Hardan, Monika Lukomska-Szymanska
Summary: This paper conducted a literature review on four microscopic methods commonly used in dental research, which are confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM). These microscopic techniques differ in their principles and require specific protocols for specimen preparation, but all play a significant role in describing and evaluating the adhesive bonding process and enamel surface condition.
Article
Dentistry, Oral Surgery & Medicine
Sunyoung Choi, Ye-Hyeon Jo, In -Sung Luke Yeo, Hyung-In Yoon, Jae-Hyun Lee, Jung-Suk Han
Summary: The purpose of this study was to investigate the effect of surface properties on biofilm formation on dental implant surfaces. The researchers found that Streptococcus gordonii formed more biofilm on titanium surfaces than zirconia surfaces, and more biofilm on rough surfaces than smooth surfaces of the same material. The proliferation of different bacteria was also affected by surface properties.
JOURNAL OF DENTAL SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Kristian Kniha, Eva Miriam Buhl, Stephan Christian Mohlhenrich, Anna Bock, Frank Holzle, Elmar Hellwig, Ali Al-Ahmad, Ali Modabber
Summary: The study evaluated the development of bacterial deposits and morphological parameters around dental zirconia and titanium implants compared with natural teeth during systemic bisphosphonate medication. Results showed that after 1 week of surgery, implants with alendronic acid had significantly more bacteria compared to natural teeth in rats with zoledronic acid administration. However, after 3 months, no significant differences were found among the groups.TEM analysis revealed differences in osteoblast activity between the control group and drug-treated animals, suggesting that bisphosphonates may impact bone cell activity around implants.
Article
Energy & Fuels
Johan Blom, Hilde Soenen, Niko Van den Brande, Wim Van den Bergh
Summary: The study investigated bee structures on bitumen surfaces using atomic force microscopy and confocal laser scanning microscopy. By simulating wax compounds, similar structures to those observed on paraffinic bitumen were created, indicating that bees and surrounding areas consist of crystallisable components.
Review
Microscopy
Georgios Pipintakos, Navid Hasheminejad, Caitlin Lommaert, Anastassiya Bocharova, Johan Blom
Summary: This review examines the microstructural changes of bitumen during aging using different microscopic techniques. The study reveals that the microstructure of bitumen evolves significantly with aging, impacting its performance. It suggests the need for a systematic sample preparation protocol to ensure consistent observations across different research groups.
Article
Microbiology
Tenzin Kunchok Lueyar, Lamprini Karygianni, Thomas Attin, Thomas Thurnheer
Summary: This study investigates the interactions between streptococcal species and the yeast Candida albicans in the development of oral biofilms. The results show that different species of streptococci have varying effects on the colony formation of C. albicans in the presence of Streptococcus mutans.
Article
Microscopy
Georgios Pipintakos, Johan Blom, Hilde Soenen, Wim Van den Bergh
Summary: This study investigates the effect of ageing on the bee structures of bitumen using Atomic Force Microscopy and Confocal Laser Scanning Microscopy. The research shows that ageing leads to a decrease in the number of bees per mu m(2) and bee area percentage, while their size increases.
Review
Infectious Diseases
Juliana Dias Corpa Tardelli, Vanderlei Salvador Bagnato, Andrea Candido dos Reis
Summary: This review examines the factors determining the strength of bacterial adhesion on titanium surfaces and its alloys. The study demonstrates that the hydrophilicity of surfaces influences bacterial adhesion, but hydrophobic bacteria can also adhere to hydrophilic surfaces through hydrogen bonding. Additionally, surface treatments that reduce areas favorable for bacterial adhesion have a greater impact on biofilm formation than surface roughness. Time-dependent studies are crucial for evaluating bacterial colonization as bacteria develop adaptation mechanisms over time.
Article
Engineering, Biomedical
Dainelys Guadarrama Bello, Patricia Moraille, Serine Boughari, Antonella Badia, Antonio Nanci
Summary: This study aims to investigate the biomechanical contribution of filopodia and their nanoprotrusions to the adhesive interaction of cells with nanostructured surfaces. The results show that surface topography can change the adhesive interaction of subcellular structures.
MATERIALS TODAY BIO
(2022)
Article
Cell Biology
Viktoria Sergunova, Vladimir Inozemtsev, Nina Vorobjeva, Elena Kozlova, Ekaterina Sherstyukova, Snezhanna Lyapunova, Aleksandr Chernysh
Summary: Confocal microscopy and fluorescence staining are commonly used to study neutrophil activation and NETosis, but they cannot reveal the specific membrane surface characteristics of neutrophils. This study used atomic force microscopy (AFM) to reveal the topography and nanosurface characteristics of neutrophils during activation and NETosis. Changes in neutrophil membrane nanosurface parameters were quantified and compared with confocal laser-scanning microscopy (CLSM) images for identification and analysis.
Article
Materials Science, Coatings & Films
P. Jeyalakshmi, P. Ramkumar
Summary: Ti6Al4V alloy is widely used in orthopaedic applications due to its excellent properties, but long-term exposure to body fluid can cause corrosion. In this study, thermal oxidation was performed on micro-blasted Ti6Al4V surfaces to minimize corrosion. The combined mechanical and thermal process increased surface roughness and improved the hydrophilic nature of the Ti6Al4V surface. Formation of metastable Ti2O3 and transformation of anatase and rutile TiO2 enhanced corrosion resistance. Micro-blasting with 48-hour thermal oxidation showed significantly higher corrosion resistance and reduced corrosion rate compared to untreated Ti6Al4V.
SURFACE & COATINGS TECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Ruben Wagner, Robert Lehnert, Enrico Storti, Lisa Ditscherlein, Christina Schroeder, Steffen Dudczig, Urs A. Peuker, Olena Volkova, Christos G. Aneziris, Horst Biermann, Anja Weidner
Summary: In this study, a combined cleaning filter system was used to treat nonmetallic inclusions in steel melt. The system was found to reduce the amount of aluminum in the steel and form novel multiphase inclusions. Compared to pure Al2O3 inclusions, these multiphase inclusions have better mechanical properties that are more suitable for steel.
MATERIALS CHARACTERIZATION
(2022)
Article
Microscopy
Johannes Mirwald, Bernhard Hofko, Georgios Pipintakos, Johan Blom, Hilde Soenen
Summary: This study utilized multiple microscopic techniques to investigate the microstructure of different binders. The results showed that these techniques were able to capture specific surface microstructures of the binders, but clear differentiation between the binders was difficult to obtain.
Article
Microbiology
Maria Villalba, Leonardo Venturelli, Ronnie Willaert, Maria E. Vela, Osvaldo Yantorno, Giovanni Dietler, Giovanni Longo, Sandor Kasas
Summary: This study utilized AFM-based nanomotion detection technique to reveal the correlation between nanometer-scale bacterial motion pattern and the virulence state of bacteria, paving the way for novel rapid and label-free pathogenic microorganism detection assays.
Article
Dentistry, Oral Surgery & Medicine
Florian Kernen, Stefan Schlager, Veronica Seidel Alvarez, Juergen Mehrhof, Kirstin Vach, Ralf Kohal, Katja Nelson, Tabea Fluegge
Summary: The accuracy of intraoral scanners is crucial for computer-aided design and computer-aided manufacturing (CAD-CAM) dentistry. This clinical study compared the accuracy of intraoral scanning with different devices to extraoral scanning. The results showed that extraoral scans had higher precision and trueness compared to intraoral scans, except for complete-arch scans with one specific intraoral scanner. Short-span scans had higher precision and trueness compared to complete-arch scans.
JOURNAL OF PROSTHETIC DENTISTRY
(2022)
Article
Chemistry, Physical
Sebastian Berthold Maximilian Patzelt, Marei Kruegel, Christian Wesemann, Stefano Pieralli, Julian Nold, Benedikt Christopher Spies, Kirstin Vach, Ralf-Joachim Kohal
Summary: The study compared conventional to digital workflows in occlusal splint production and found that the digital workflow was more time efficient and led to better fit than the conventional workflow.
Letter
Immunology
Martin Wolkewitz, Oksana Martinuka
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Chemistry, Physical
Aimen Bagegni, Vincent Weihrauch, Kirstin Vach, Ralf Kohal
Summary: The use of screwless Morse taper IAC can eliminate mechanical complications and provide stable implant-abutment connections. Screw-retained hexagonal IAC showed the highest retention stability, while screwless Morse taper IACs remained stable for extended periods of time, making them a valid treatment option for single-tooth replacement.
Article
Engineering, Biomedical
Kerstin Rabel, Julian Nold, Daniela Pehlke, James Shen, Anze Abram, Andraz Kocjan, Siegbert Witkowski, Ralf -Joachim Kohal
Summary: This study compared self-glazed zirconia restorations with conventionally CAD/CAM milled and glazed controls and found that self-glazed zirconia restorations have higher surface microstructural homogeneity and fracture strength. The fracture strength of self-glazed zirconia restorations decreased after dynamic loading but remained significantly higher than the conventionally glazed controls. Therefore, this study suggests that self-glazed zirconia restorations are stable for clinical application and further research is needed to prove their clinical applicability.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Chemistry, Physical
Babak Saravi, Anselm Flohr, Sebastian B. Patzelt, Benedikt C. Spies, Derek Hazard, Ralf J. Kohal
Summary: This study evaluated the physico-mechanical properties of PEEK abutments as implant dentistry materials using a chewing simulator model. By comparing surface roughness, microgaps, and fracture loads between PEEK abutments and zirconia abutments, it was found that PEEK abutments performed better in load-bearing properties and could be a feasible alternative to zirconia abutments.
Article
Chemistry, Physical
Malin Strasding, Samir Abou-Ayash, Thomas Laziok, Sam Doerken, Ralf-Joachim Kohal, Sebastian Berthold Maximilian Patzelt
Summary: The purpose of this study was to analyze the clinical and patient-reported outcomes of patients restored with non-precious metal alloy double crown-retained removable partial dentures (NP-D-RPDs). The results showed that non-precious metal alloy partially veneered NP-D-RPDs are an efficient alternative to precious metal alloy RPDs with excellent patient-reported outcomes.
Article
Dentistry, Oral Surgery & Medicine
Ralf-Joachim Kohal, Christopher von Schierholz, Julian Nold, Benedikt C. C. Spies, Erik Adolfsson, Kirstin Vach, Felix Burkhardt
Summary: The objective of this study was to investigate the fracture strength and potential phase transformation of an injection-molded two-piece zirconia implant restored with a zirconia abutment after loading and/or aging. The results showed that dynamic loading and the combination of loading and aging reduced the fracture strength of the implant abutment combination. Hydrothermal treatment caused a shallow transformation zone which had no influence on the fracture strength.
CLINICAL ORAL IMPLANTS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Alexander Welle, Kerstin Rabel, Matthias Schwotzer, Ralf Joachim Kohal, Thorsten Steinberg, Brigitte Altmann
Summary: With the increasing use of zirconia-based nano-ceramics in dentistry, it is important to examine the potential biological effects of released nanoparticles on oral tissues like bone. This study used ToF-SIMS to investigate the internalization of differently sized zirconia nanoparticles (ZrNP) into human osteoblasts and found that ZrNP were internalized by the cells in a size-dependent manner. The study also showed that exposure to ZrNP had a negative effect on the metabolic activity of the cells, with the smallest ZrNP having the most pronounced effect.
Article
Engineering, Biomedical
Ralf-Joachim Kohal, Kirstin Vach, Frank Butz, Benedikt Christopher Spies, Sebastian Berthold Maximilian Patzelt, Felix Burkhardt
Summary: The study investigated the clinical and radiological outcome of one-piece zirconia oral implants supporting three-unit fixed dental prostheses after three years. 54 implants were placed in 27 patients through a one-stage surgery and immediate provisionalization. Standardized radiographs were taken at different time points to evaluate bone loss and soft-tissue parameters. The zirconia implants exhibited a cumulative survival rate of 98.1% after three years, with a mean marginal bone loss of 2.16 mm. However, high peri-implantitis incidence and low implant success rate were observed, indicating a possible concern for long-term stability.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Michael Rassner, Silvia Waldeck, Marie Follo, Stefanie Jilg, Ulrike Philipp, Martina Jolic, Julius Wehrle, Philipp J. Jost, Christian Peschel, Anna Lena Illert, Justus Duyster, Florian Scherer, Nikolas von Bubnoff
Summary: This study developed a set of ddPCR assays for the detection of cKIT mutations mediating resistance to cKIT kinase inhibitors in ctDNA. The assays showed good analytical sensitivity and specificity, and demonstrated high correlation with next generation sequencing results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Ralf-Joachim Kohal, Felix Burkhardt, Jerome Chevalier, Sebastian Berthold Maximilian Patzelt, Frank Butz
Summary: This 5-year prospective cohort investigation evaluated the outcomes of a one-piece zirconia implant system for single tooth replacement. Follow-ups showed a cumulative implant survival rate of 78.2% after 5 years. Patient satisfaction was higher compared to pre-treatment measurements.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Ralf-Joachim Kohal, Anja Trinkner, Felix Burkhardt, Sebastian Berthold Maximilian Patzelt, Kirstin Vach, Monika Kuster, Anze Abram, Andraz Kocjan, Julian Nold
Summary: The aim of this study was to evaluate the long-term stability of one-piece diameter reduced zirconia oral implants under loading and artificial aging. The results showed that dynamic loading, hydrothermal aging, and the combination of loading and aging did not significantly affect the stability of the implants. The implant system was able to resist physiological chewing forces and maintain stability over a long service period.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Babak Saravi, Julia Ilbertz, Kirstin Vach, Ralf J. Kohal, Sebastian B. M. Patzelt
Summary: Computerized optical impression making (COIM) is a rising trend in dental prosthetics for removable dentures, but its accuracy compared with traditional impression-making techniques remains uncertain. A study evaluating the accuracy of COIM in partially edentulous jaws found that it captured dental hard tissue more accurately than mucosal areas, likely due to software algorithms filtering out mobile tissues. Clinically, caution is needed when using COIM for prosthetics involving mucosal tissues as deviations could compromise the fit and longevity.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Chemistry, Physical
Aimen Bagegni, Justus Borchers, Samuel Beisel, Sebastian B. M. Patzelt, Kirstin Vach, Ralf Kohal
Summary: This study evaluated the effects of different luting agents on the bonding between zirconium dioxide crowns and titanium bonding bases. The results showed that most luting agents were suitable for cementation of zirconium dioxide crowns, meeting the recommended debonding force value.