Article
Biotechnology & Applied Microbiology
Rucha Deshpande, Swati Shukla, Raeesa Sayyad, Shalmali Salunke, Anuya Nisal, Premnath Venugopalan
Summary: This study compared the safety and efficacy of silk fibroin-based bone void fillers with ceramic fillers and evaluated their impact on stem cell differentiation. Results demonstrated that SF scaffolds promoted a better differentiation of stem cells into osteoblast lineage compared to ceramic scaffolds.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ho-Kyung Lim, Ik-Jae Kwon, Sung-Woon On, Seok-Jin Hong, Byoung-Eun Yang, Soung-Min Kim, Jong-Ho Lee, Soo-Hwan Byun
Summary: The study investigated the bone regeneration capacity of BCP powder, BCP block, and BCP collagen with different concentrations of rhBMP-2 in an animal model. Results showed that increasing rhBMP-2 concentration did not significantly enhance osteogenesis, while collagen-added material may help mediate rapid bone formation in initial stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Ceramics
Yessie W. Sari, Annisa Tsalsabila, Angga Saputra, Nur Aisyah Nuzulia, Yuliati Herbani
Summary: Surface modification of biomaterials affects the surface reactivity of implantable materials and induces the formation of apatite-like layers. This study investigates the bioactivity and surface reactivity of modified biomaterials (calcium-deficient hydroxyapatite [CDHA] on amino acid-capped AuNPs [AA-AuNPs]) by immersing them in simulated body fluid (SBF). The results show that AA-AuNPs, capped with charged and polar uncharged amino acids, are capable of inducing biomimetic hydroxyapatite formation. The presence of hydroxyapatite peaks in XRD patterns confirms the formation of hydroxyapatite after immersion in SBF. Furthermore, longer immersion time leads to nucleation and growth of hydroxyapatite, as indicated by changes in crystal structure. The surface modification of AA-AuNPs significantly affects the orientation of hydroxyapatite crystals and the presence of charged amino acids contributes to nucleation and growth, while polar uncharged amino acids only contribute to growth. The study highlights the bioactivity of CDHA-modulated AA-AuNPs in hydroxyapatite formation for bone tissue regeneration.
CERAMICS INTERNATIONAL
(2023)
Article
Engineering, Biomedical
Rebu Sundar, Josna Joseph, Suresh Babu, Harikrishna Varma, Annie John, Annie Abraham
Summary: This experimental study compared the physical characteristics of different bioceramics and evaluated the activity and differentiation of rabbit adipose-derived mesenchymal stem cells on these ceramic substrates. Strontium-doped hydroxyapatite (SrHA) was found to significantly enhance the differentiation of mesenchymal stem cells towards bone lineage, making it a promising material for hard tissue implantation and drug delivery.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Materials Science, Biomaterials
Martyna Nikody, Jiaping Li, Elizabeth Rosado Balmayor, Lorenzo Moroni, Pamela Habibovic
Summary: This article introduces a polymer-ceramic composite material that enhances its osteogenic properties by adding zinc, for the treatment of critical-sized bone defects that cannot heal spontaneously and require surgical intervention. The study found that the addition of zinc can promote the differentiation of osteogenic cells, enhance extracellular matrix production and mineralization, and demonstrate the potential for treating critical-sized bone defects.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Julia Gao, Yingchao Su, Yi-Xian Qin
Summary: This study compared the in vitro and in vivo degradation and biocompatibility behaviors of magnesium alloys with calcium phosphate coatings, showing that the CaP coating significantly improved biodegradation behavior and biocompatibility of the Mg alloy. In vitro tests were found to be a quick screening tool for biodegradation rate, with the in vivo degradation rate being slower than in vitro immersion degradation rate.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Anastasia Yu. Teterina, Igor V. Smirnov, Irina S. Fadeeva, Roman S. Fadeev, Polina V. Smirnova, Vladislav V. Minaychev, Margarita I. Kobyakova, Aleksandr Yu. Fedotov, Sergey M. Barinov, Vladimir S. Komlev
Summary: The study presented the synthesis of low-temperature calcium phosphate compounds with strontium substitution to improve bioactive properties, indicating the effectiveness of using strontium in OCP for enhancing biocompatibility in composite materials intended for bone repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Chia-Jen Wu, Kang-Fan Liu, Chung-Ming Liu, Wen-Chien Lan, Shu-Fen Chu, Yung-Kang Shen, Bai-Hung Huang, Jonathan Huang, Yung-Chieh Cho, Keng-Liang Ou, Pei -Wen Peng
Summary: This study successfully developed a highly pure and crystalline beta-tricalcium phosphate (b-TCP) bone graft substitute using the propagated Scleractinian coral. The coral-derived b-TCP with interconnected microporosity exhibited favorable biocompatibility and proper resorbed properties, showing promise as a bone graft substitute for bone defect repair in dental and orthopedic fields.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2023)
Article
Chemistry, Multidisciplinary
Caroline J. Harrison, Paul Hatton, Piergiorgio Gentile, Cheryl A. Miller
Summary: Nanoscale SrHA materials were successfully prepared using wet precipitation and sol-gel methods, showing increased radiopacity and superior biocompatibility for applications in bone surgery.
Article
Engineering, Biomedical
Jiangyu Cai, Qianqian Zhang, Jiebo Chen, Jia Jiang, Xiumei Mo, Chuanglong He, Jinzhong Zhao
Summary: The study evaluated the effects of biomineralization and electrodeposition methods for depositing calcium-phosphate on PET artificial ligaments. Results showed that ED-CaP had more uniform particles and element distribution, enhancing osteogenic differentiation of cells and graft-bone integration. Overall, electrodeposition of CaP is an effective strategy to modify PET artificial ligaments and improve graft-bone integration both in vitro and in vivo.
BIOACTIVE MATERIALS
(2021)
Article
Materials Science, Ceramics
Yun-Hee Lee, Ji-Won Lee, Seo-Young Yang, Hyun Lee, Young Hag Koh, Hyoun-Ee Kim
Summary: This study introduces a novel type of biphasic calcium phosphate (BCP) gyroid scaffolds with unique structure and microporous walls, produced using PMMA microspheres as porogen for ceramic manufacturing technique. By controlling the PMMA content, the microporosity of the BCP walls and the overall porosity of the scaffolds were adjusted, resulting in high overall porosity while maintaining reasonable compressive strength.
CERAMICS INTERNATIONAL
(2021)
Article
Chemistry, Physical
J. Alipal, S. Saidin, A. Z. Kai Lo, P. Koshy, H. Z. Abdullah, M. I. Idris, T. C. Lee
Summary: This study investigated the effects of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity. Two different CaP/TiO2 micromorphology-based coatings were created using single-step micro-arc anodic oxidation at different conditions, namely donut-shaped (350 V) and needle-shaped (450 V), and their cytocompatibility and antibacterial properties were examined. The results showed that the needle-shaped coating at 450 V was mechanically unstable and had long-term negative implications on bone growth. The donut-shaped coating at 350 V had superior antimicrobial effects and was the best interface for bone cell anchorage.
SURFACES AND INTERFACES
(2023)
Article
Orthopedics
Nikola Stokovic, Natalia Ivanjko, Viktorija Rumenovic, Anita Breski, Kuber T. Sampath, Mihaela Peric, Marko Pecina, Slobodan Vukicevic
Summary: This study evaluated different commercially available synthetic ceramic particles as compression-resistant matrix (CRM) added to Osteogrow implants. The results showed that Osteogrow-C implants with different ceramic particles induced the formation of bone-ceramic structure and promoted bone formation. Highly porous Vitoss ceramic demonstrated the highest bone volume, while Vitoss and ChronOs ceramics increased the number of trabeculae, and BAM and Dongbo ceramics increased the thickness of trabeculae.
INTERNATIONAL ORTHOPAEDICS
(2022)
Article
Chemistry, Multidisciplinary
Rafael Otero-Perez, Maria Permuy, Estefania Lopez-Senra, Miriam Lopez-Alvarez, Monica Lopez-Pena, Julia Serra, Antonio Gonzalez-Cantalapiedra, Fernando M. Munoz, Pio Gonzalez
Summary: The research compared the effectiveness of a marine-derived shark tooth bone graft with a commercial bovine-derived bone graft in a rabbit model, demonstrating that the shark tooth bone graft has better biocompatibility and bone regeneration stimulation.
APPLIED SCIENCES-BASEL
(2021)
Review
Engineering, Biomedical
Oznur Demir-Oguz, Aldo R. Boccaccini, Dagnija Loca
Summary: Calcium phosphate bone cements (CPCs) have valuable properties such as bioactivity, osteoconductivity, injectability, and moldability, but their low mechanical performance limits their clinical application. Adding bioactive glasses (BGs) as a secondary powder phase can improve the injectable properties of CPCs and enhance the degradation rate, in vitro osteogenic differentiation, cell response, and tissue-material interaction.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
B. Le Gars Santoni, L. Niggli, S. Dolder, O. Loeffel, G. A. Sblendorio, R. Heuberger, Y. Maazouz, C. Staehli, N. Doebelin, P. Bowen, W. Hofstetter, M. Bohner
Summary: This study assessed the effect of small fractions of β-CPP on the in vitro osteoclastic resorption of β-TCP and found that β-CPP fractions significantly inhibited the resorption of β-TCP. The study also examined the impact of β-CPP and HA impurities on the physico-chemical properties of β-TCP powders and sintered cylinders.
BIOACTIVE MATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Andreas Fuchs, Dorothea Kreczy, Theresa Bruckner, Uwe Gbureck, Philipp Stahlhut, Melanie Bengel, Andreas Hoess, Berthold Nies, Julia Bator, Uwe Klammert, Christian Linz, Andrea Ewald
Summary: The study demonstrates that CaMgP granules exhibit excellent biocompatibility, fast and continuous degradation, and enable proper bone healing without the interposition of connective tissue. These characteristics make CaMgP granules a promising bone substitute material for bone augmentation procedures, especially in sensitive areas.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Engineering, Biomedical
Theodor Kuehling, Pia Schilling, Anke Bernstein, Hermann O. Mayr, Annerose Serr, Annette Wittmer, Marc Bohner, Michael Seidenstuecker
Summary: The aim of this study was to establish an organ model for staphylococcal infection of human bone samples and investigate the effectiveness of a microporous ceramic loaded with hydrogels and clindamycin. The results showed that the bacterial count significantly decreased in the groups treated with ADA-gelatin + clindamycin and alginate + clindamycin, while it remained constant in the control groups. There were no significant differences in bone vitality among the groups.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Friederike Kaiser, Lena Schroeter, Svenja Stein, Benjamin Krueger, Jan Weichhold, Philipp Stahlhut, Anita Ignatius, Uwe Gbureck
Summary: Results of several studies have shown that magnesium phosphate cements (MPCs) have excellent biocompatibility, osteoconductivity, and higher solubility compared to calcium phosphates, making them promising alternatives to slowly degrading calcium phosphate cements. Decreasing the powder-liquid ratio (PLR) of the cement can further improve the degradation speed, with the potential for fast bone ingrowth within a few months. However, further modifications may be needed, especially for the K-struvite cement, to achieve a slower resorption and reduced inflammatory response in vivo.
ACTA BIOMATERIALIA
(2022)
Review
Engineering, Biomedical
Marc Bohner, Yassine Maazouz, Maria-Pau Ginebra, Pamela Habibovic, Jonathan G. Schoenecker, Howard Seeherman, Jeroen J. J. P. van den Beucken, Frank Witte
Summary: This study aims to evaluate the hypothesis that sustained local ionic homeostatic imbalance (SLIHI) created by mineral formation during tissue calcification modulates inflammation to trigger heterotopic ossification (HO), and to provide insights for the design of cell-free, drug-free osteoinductive bone graft substitutes.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Annika Seifert, Tina Tylek, Carina Blum, Naomi Hemmelmann, Bettina Boettcher, Uwe Gbureck, Juergen Groll
Summary: This study investigated the response of human macrophages to different CaPs and pore structures. The study found that macrophages are capable of releasing ETs in biomaterials, and METs associated with calcium deficient hydroxyapatite were significantly weaker or absent.
Article
Engineering, Biomedical
Michaela Roedel, Joerg Tessmar, Juergen Groll, Uwe Gbureck
Summary: A novel dual setting brushite-gelatin cement with genip-initiated cross-linking of gelatin was developed. The addition of gelatin hydrogel with a concentration of 10.0 w/v% improved the elastic properties, although it resulted in a decrease in compressive strength. The composites with a powder-to-liquid ratio of 2.5 g*mL(-1) exhibited pseudo-ductile fracture behavior during compression testing.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2022)
Article
Engineering, Biomedical
Philipp Heilig, Martin Cornelius Jordan, Mila Marie Paul, Eva Kupczyk, Rainer Heribert Meffert, Uwe Gbureck, Stefanie Hoelscher-Doht
Summary: The study shows that the pullout strength and stiffness of suture anchors can be significantly increased in bone by filling it with magnesium phosphate cement. SwiveLock C anchors perform better when reinforced with additional FibreWire.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Instruments & Instrumentation
Jussi Petteri Suuronen, Bernhard Hesse, Max Langer, Marc Bohner, Julie Villanova
Summary: This study presents a methodology for evaluating the image quality of in-line phase contrast nanoCT images of bone scaffold samples. By exploring and quantitatively comparing the effects of implementation choices in the imaging setup, such as camera technology and imaging energy, on the resulting image quality, it provides important insights for further understanding the integration of implants into bone tissue.
JOURNAL OF SYNCHROTRON RADIATION
(2022)
Article
Pharmacology & Pharmacy
Lena Scheller, Stephanie Bachmann, Theresa Zorn, Simon Hanio, Uwe Gbureck, Dimitrios Fatouros, Ann-Christin Poppler, Lorenz Meinel
Summary: In this study, the solidification of drug-loaded microemulsion preconcentrates with the acid-sensitive metal-organic framework ZIF-8 was detailed using various analytical techniques. Nile Red, Vitamin K1, and Lumefantrine were used as model compounds to analyze the adsorption and desorption dynamics. The loaded preconcentrates and drugs were successfully preserved in the crystal structure of ZIF-8, which could be pressed into tablets or 3D-printed into oral dosage forms. The stimuli-responsive metal organic frameworks have the potential to form colloidal microemulsions in solid dosage forms for oral delivery of lipid-based formulations.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Engineering, Biomedical
Yassine Ouhaddi, Baptiste Charbonnier, Juliette Porge, Yu-Ling Zhang, Isadora Garcia, Uwe Gbureck, Liam Grover, Mirko Gilardino, Edward Harvey, Nicholas Makhoul, Jake Barralet
Summary: In this study, we investigated the response of blood vessels to surgical injury or placement of an inorganic construct, and found that only calcium phosphate induced a significant angiogenic response. We also identified changes in macrophage and smooth muscle cell populations, as well as the activation of NLRP3 inflammasome and sustained expression of IL-1 beta. These findings suggest a potential role of sterile inflammation in biomaterial-induced angiogenesis, and provide a new strategy for creating better pro-regenerative biomaterials.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Bastien Le Gars Santoni, Luzia Niggli, Silvia Dolder, Olivier Loeffel, Gabrielle A. Sblendorio, Yassine Maazouz, Duncan T. L. Alexander, Roman Heuberger, Christoph Staehli, Nicola Doebelin, Paul Bowen, Willy Hofstetter, Marc Bohner
Summary: This study investigated how a change of sintering atmosphere could affect the physico-chemical and biological properties of β-TCP. The results showed that sintering β-TCP in a monetite-rich atmosphere reduced the grain size and increased density, but led to a lower Ca/P molar ratio at the surface and decreased osteoclastic resorption.
ACTA BIOMATERIALIA
(2023)
Article
Dentistry, Oral Surgery & Medicine
Andreas Fuchs, Michael Bartolf-Kopp, Hartmut Boehm, Anton Straub, Alexander C. C. Kuebler, Christian Linz, Uwe Gbureck
Summary: Composite grafts made of self-setting and oil-based calcium phosphate cement (CPC) pastes, reinforced with 3D-printed polycaprolactone (PCL) fiber mats, can be adapted to various bony defects in oral and maxillofacial surgery. The PCL-reinforced CPC samples have good processability and a high precision of fit, with increased clinical handling properties.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Engineering, Biomedical
Tobias Renner, Paul Otto, Alexander C. Kuebler, Stefanie Hoelscher-Doht, Uwe Gbureck
Summary: A bone adhesive for use in surgeries is currently not available. Phosphoserine modified cements (PMC) based on mixtures of OPLS and calcium phosphates, as well as chelate setting magnesium phosphate cements, have gained popularity. New mineral-organic bone cements based on phosphoserine and magnesium phosphates or oxides were investigated for their adhesive properties. The novel adhesives demonstrated excellent bond strength to bone and outperformed currently available adhesives.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2023)
Meeting Abstract
Endocrinology & Metabolism
Franziska Strunz, Mark Siegrist, Nikola Saulacic, Marc Bohner, Willy Hofstetter
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)