期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 103, 期 4, 页码 1533-1545出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.35290
关键词
gelatin sponge scaffold; neural stem cells; neurotrophin-3; TrkC; spinal cord injury
资金
- Chinese National Natural Science Foundation [81330028, U1301223]
- National 863 Project [2013AA020106]
- Foundation of the Education Ministry of China [201300193035]
- Foundation of Guangdong Province [2011A030300004]
- Foundation of Guangzhou City [2012J4100077]
Biological materials combined with genetically-modified neural stem cells (NSCs) are candidate therapy targeting spinal cord injury (SCI). Based on our previous studies, here we performed gelatin sponge (GS) scaffold seeded with neurotrophin-3 (NT-3) and its receptor TrkC gene modifying NSCs for repairing SCI. Eight weeks later, compared with other groups, neurofilament-200 and 5-hydroxytryptamine positive nerve fibers were more in the injury site of the N+T-NSCs group. Immunofluorescence staining showed the grafted NSCs could differentiate into microtubule associated protein (Map2), postsynaptic density (PSD95), and mouse oligodendrocyte special protein (MOSP) positive cells. The percentage of the Map2, PSD95, and MOSP positive cells in the N+T-NSCs group was higher than the other groups. Immuno-electron microscopy showed the grafted NSCs making contact with each other in the injury site. Behavioral analysis indicated the recovery of hindlimbs locomotion was better in the groups receiving cell transplant, the best recovery was found in the N+T-NSCs group. Electrophysiology revealed the amplitude of cortical motor evoked potentials was increased significantly in the N+T-NSCs group, but the latency remained long. These findings suggest the GS scaffold containing genetically-modified NSCs may bridge the injury site, promote axon regeneration and partial functional recovery in SCI rats. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1533-1545, 2015.
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