Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alvaro Yogi, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, Maria J. Moreno
Summary: The study found that VSMCs differentiated from adipose-derived stem cells (ASCs) show potential for populating PET-based vascular scaffolds. By shortening the differentiation period and studying the role of downstream effectors of TGF-beta, the process was stabilized. The non-woven PET structure did not affect ASC differentiation, providing a novel alternative to address issues with long-term patency of vascular grafts.
Article
Medicine, Research & Experimental
Francesca Toia, Elena Lo Presti, Anna Barbara Di Stefano, Marta Di Simone, Marco Trapani, Anna Maria Corsale, Carmela Picone, Francesco Moschella, Francesco Dieli, Adriana Cordova, Serena Meraviglia
Summary: This study analysed the immunomodulatory properties of spheroids from adipose stem cells (SASCs) and compared them to adipose stem cells (ADSCs). SASCs showed stronger immunomodulatory abilities in heterologous experimental settings compared to ADSCs. SASCs also demonstrated greater anti-inflammatory properties.
Article
Cell & Tissue Engineering
Richard J. P. Smith, Alessandro Faroni, James R. Barrow, Jamie Soul, Adam J. Reid
Summary: Enriching grafts with the CD271+ AD-MSC subpopulation has the potential to improve reconstructive and aesthetic surgeries involving adipose tissue, as these subpopulations express higher levels of angiogenic genes and lower levels of inflammatory genes, possess typical stem cell surface markers, and are more likely to promote effective neoangiogenesis than CD271- AD-MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Federica Grilli, Ennio Albanesi, Beatriz Pelacho, Felipe Prosper, Paolo Decuzzi, Daniele Di Mascolo
Summary: Depositing stem cells at the injury site is a clinically relevant strategy for tissue repair and angiogenesis, but insufficient cell engraftment and survival requires the development of new scaffolds. In this study, a microstructured poly(lactic-co-glycolic acid) (PLGA) fabric was investigated as a promising biodegradable scaffold for adipose-derived stem cell (ADSC) tissue integration. The results showed that ADSCs reassembled into spheroidal-like structures on the PLGA fabric, preserving cell viability and promoting a nonlinear actin organization. Moreover, the secretion of specific factors involved in angiogenesis, extracellular matrix remodeling, and stem cell homing was enhanced on the PLGA fabric compared to conventional substrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Orthopedics
Xing Guo, Denghua Huang, Dan Li, Longfei Zou, Hui Lv, Yuanhui Wang, Meiyun Tan
Summary: This study found that hypoxia preconditioning attenuates the proliferation and migration ability of ADSCs, but has positive impact on tenogenic differentiation through HIF-1 alpha signaling pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Materials Science, Multidisciplinary
Danli Zhu, Wanting Bao, Boxuan Wei, Hao Wei, Jinwu Wang, Guangdong Zhou, Xiansong Wang, Shanyu Guo
Summary: In this study, a strategy for the construction of vascularized tissue-engineered adipose tissue was proposed. By integrating a gas-foamed method (soil) and three-dimensional cell spheroids (seeds), adipose tissue regeneration was achieved. The prepared soil significantly enhanced the vascularization of the regenerated tissue, while optimizing the seed cells promoted adipogenesis. This study provides a solution for the regeneration of vascularized adipose tissue and establishes a general research model for the reconstruction of breast defects.
APPLIED MATERIALS TODAY
(2023)
Article
Engineering, Biomedical
Huijuan Fu, Dequan Zhang, Jinshi Zeng, Qiang Fu, Zhaoyang Chen, Xuer Sun, Yi Yang, Shiyi Li, Minliang Chen
Summary: This study aimed to explore the in vivo application of a 3D-printed tissue-engineered skin substitute loaded with human adipose-derived stem cells (hADSCs) in wound healing. The newly designed biomaterial, composed of adipose tissue decellularized extracellular matrix (dECM) pre-gel, methacrylated gelatin (GelMA), and methacrylated hyaluronic acid (HAMA), was fabricated by 3D printing. The results showed that the 3D-printed skin substitute accelerated wound healing, promoting blood perfusion, re-epithelialization, collagen deposition, and angiogenesis.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Cell Biology
Maria Giovanna Scioli, Gabriele Storti, Alessandra Bielli, Massimo Sanchez, Manuel Scimeca, Jeffrey M. Gimble, Valerio Cervelli, Augusto Orlandi
Summary: The study reveals the crucial role of CD146 in ASC properties and osteochondrogenic commitment, with CD146(+) ASCs showing superior chondrogenic potential and CD146(-) ASCs demonstrating greater osteogenic capacity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Engineering, Biomedical
Tong Liu, Jia Xu, Xun Pan, Zhangfan Ding, Hao Xie, Xiaoyi Wang, Huixu Xie
Summary: Autologous tissue transplantation is an effective method for restoring oral and maxillofacial functions and aesthetics, but faces limitations such as complications from donor sites, limited tissue volume, and uncertain long-term outcomes. Adipose-derived mesenchymal stem cells (ADMSCs) have multilineage differentiation capabilities and are important for oral and maxillofacial tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Moema de Alencar Hausen, Ariana de Souza Moraes, Flavia Pedrini, Felipe Grabarz, Jose Angelo Camilli, Eliana Aparecida de Rezende Duek
Summary: This study introduces a natural biomaterial device that can improve the wound healing process by modulating macrophage behavior in vitro. The device, assembled with adipose-derived stem cells and biocompatible polymers, promotes the release of anti-inflammatory cytokines, indicating the prevalence of M2 macrophages.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Cell & Tissue Engineering
Nicolo C. Brembilla, Hubert Vuagnat, Wolf-Henning Boehncke, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: Chronic wounds have a significant impact on quality of life and healthcare costs. Adipose-derived stromal cells (ASC) have emerged as a promising therapy for chronic wounds. Studies have shown the clinical efficacy of ASC-based therapies in treating chronic wounds.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Aida Selaru, Alexandra-Elena Mocanu-Dobranici, Elena Olaret, Raluca-Elena Ginghina, Izabela-Cristina Stancu, Marieta Costache, Sorina Dinescu
Summary: In this study, fibrous nanostructured substrates based on fish gelatin enriched with graphene oxide and magnetic nanoparticles were fabricated. The biological properties of these substrates, including cell viability, adhesion, and differentiation, were evaluated. The results showed good interaction between the materials and cells, indicating their potential for tissue engineering applications, with a concentration of 0.5% graphene oxide and magnetic nanoparticles being the most suitable option for biological testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)