Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ferdy K. Cayami, Lauria Claeys, Ruben de Ruiter, Bernard J. Smilde, Lisanne Wisse, Natalija Bogunovic, Elise Riesebos, Lyra Eken, Irsan Kooi, Erik A. Sistermans, Nathalie Bravenboer, Gerard Pals, Sultana M. H. Faradz, Daoud Sie, E. Marelise W. Eekhoff, Dimitra Micha
Summary: We developed a highly efficient platelet lysate-based approach to directly transdifferentiate skin-derived human fibroblasts to osteoblast-like cells. The transdifferentiated cells showed increased expression of osteoblast-specific markers and exhibited osteogenic properties. RNA-seq analysis revealed distinct transcriptome profiles between the transdifferentiated cells and primary fibroblasts. This in vitro model may contribute to studying osteoblast-dependent disorders in patient-derived cells.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Dentistry, Oral Surgery & Medicine
Wen Wen, Yu Pang, Yuyang Tian, Chunmei Xu, Jun Wang, Yafei Wu, Xudong Xie
Summary: Periodontitis causes tooth loss through destruction of alveolar bone. Understanding the mesenchymal stem cell (MSC) populations involved in alveolar bone formation can contribute to regenerative therapies. MSCs in bone marrow, periosteum, periodontal ligament (PDL), and gingiva are considered osteogenic progenitors. Specific markers like Gli1, Axin2, PTHrP, LepR, and alpha-SMA have been studied to understand their contribution to alveolar bone formation. This review discusses the MSCs involved in alveolar bone development, remodeling, injury repair, and regeneration.
Article
Biochemistry & Molecular Biology
Nan Wang, Guoli Gan, Jihao Yang, Luyao Wang
Summary: This study found that barbaloin promotes the osteogenic differentiation of human bone marrow mesenchymal stem cells, possibly through the regulation of the Wnt/beta-catenin signaling pathway.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Chenglong Wang, Sabine Stoeckl, Shushan Li, Marietta Herrmann, Christoph Lukas, Yvonne Reinders, Albert Sickmann, Susanne Graessel
Summary: This study investigated the effects of extracellular vesicles (EVs) isolated from different stages of human bone marrow mesenchymal stem cells (hBMSCs) on the osteogenic and adipogenic differentiation capacity of naive hBMSCs. The results showed that EVs from late-stage osteogenic differentiated hBMSCs promoted osteogenic potential in naive hBMSCs and inhibited their adipogenic differentiation. Proteomic analysis revealed distinct protein profiles in EVs from osteogenic and naive hBMSCs.
Article
Biotechnology & Applied Microbiology
Lei Qi, Weiwen Ge, Cancan Pan, Weidong Jiang, Dan Lin, Lei Zhang
Summary: Stem cell senescence causes functional declines and disrupts bone environment homeostasis. Stem cell-derived exosomes are a promising therapy for aging-related osseous diseases. This study demonstrates the potential of osteoinductive exosomes in bone repair for aging individuals and highlights the importance of considering endocytic mechanisms and avoiding negative effects in future therapeutic design for aging populations.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Salma El-Habashy, Hoda Eltaher, Ahmed Gaballah, Radwa Mehanna, Amal H. El-Kamel
Summary: This study successfully developed a novel composite nanoparticle system that integrates doxycycline into biomaterials to improve its cytotoxicity and enhance osteogenic activity. The experimental results showed that this composite system can control the release of doxycycline and significantly ameliorate its toxicity to bone marrow stem cells, while promoting the maturation of osteogenic cells.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Pharmacology & Pharmacy
Daniel Garcia-Sanchez, Alberto Gonzalez-Gonzalez, Patricia Garcia-Garcia, Ricardo Reyes, Maria Isabel Perez-Nunez, Jose A. Riancho, Carmen Evora, Jose Carlos Rodriguez-Rey, Flor M. Perez-Campo
Summary: The study demonstrates a method to enhance the osteogenic potential of MSCs by modulating the expression levels of specific genes. It was found that using Locked Nucleic Acid-Antisense Oligonucleotides and a scaffold releasing low doses of BMP-2 can increase the osteogenic potential of MSCs. By specifically targeting the Wnt pathway modulator Sfrp1, the efficiency of MSC bone regeneration can be improved and osteogenic differentiation in osteoporotic patients' MSCs can be induced.
Article
Cell & Tissue Engineering
Zhenqing Liu, Jin Sook Suh, Peng Deng, Olga Bezouglaia, Megan Do, Mojan Mirnia, Zhong-Kai Cui, Min Lee, Tara Aghaloo, Cun-Yu Wang, Christine Hong
Summary: Nerve growth factor (NGF) is known for its role in nervous system development and cell survival/differentiation. Recent studies have found that NGF also enhances bone regeneration. This study shows that NGF mediates osteogenic differentiation through the p75(NTR) pathway, activating the JNK cascade and KDM4B expression to remove repressive epigenetic marks and promote the expression of DLX5, a master osteogenic gene. KDM4B and c-Jun from the JNK signaling pathway work together to regulate NGF-mediated osteogenic differentiation.
Article
Oncology
J. F. Huo, M. L. Zhang, X. X. Wang, D. H. Zou
Summary: The study found that chrysin can promote osteogenic differentiation of DPSCs and enhance DPSC-based bone formation in nude mice and rat models. Proteomics analysis revealed the crucial role of Smad3 in this process.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Engineering, Biomedical
Ho-Ying Wan, Rita Lih Ying Shin, Jack Chun Hin Chen, Marisa Assuncao, Dan Wang, Susie K. Nilsson, Rocky S. Tuan, Anna Blocki
Summary: The study demonstrated that supplementation of Dextran sulfate (DxS) in osteogenically induced cultures of human mesenchymal stem cells (MSCs) amplified the deposition of osteoblastic extracellular matrix (ECM). This enhancement led to increased collagen type I deposition and osteogenic gene expression, as well as enrichment in bone morphogenetic protein 2 (BMP-2), suggesting a potential role of DxS in promoting osteogenic differentiation by MSCs.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Paulina Kazimierczak, Malgorzata Koziol, Agata Przekora
Summary: The newly developed biomaterial showed high biocompatibility and influenced macrophage polarization and osteoblast differentiation, playing an immunomodulatory role. The study demonstrated a low risk of inflammatory response and induced macrophage polarization into the osteogenic phenotype M2, making it a promising bone scaffold for regenerative medicine applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Environmental
Yi Guo, Li Zhou, Min Wang, Yannan Li, Bo Lei
Summary: A novel antibacterial non-viral vector based on branched glycerol-polypeptide polymer (GEPL) was developed for efficient miR-29b delivery to regulate osteogenic differentiation of bone marrow stem cells (BMSCs) and in vivo bone regeneration. Compared to other vectors, GEPL showed excellent biocompatibility and significantly improved bone tissue regeneration in vivo.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Min Wang, Tianming Dai, Qingqi Meng, Wen Wang, Siming Li
Summary: This study aimed to investigate the regulatory effects of miR-28 on osteogenic differentiation of hBMMSCs. It was found that miR-28 could inhibit STAT1 expression, upregulate AKP and RUNX2 expression, and promote osteogenic differentiation.
Article
Polymer Science
Diego O. Sanchez Ramirez, Cinzia Tonetti, Iriczalli Cruz-Maya, Vincenzo Guarino, Roberta Peila, Riccardo A. Carletto, Alessio Varesano, Claudia Vineis
Summary: This study investigates the effects of pH on the properties of cysteine-S-sulfonated keratin, including secondary structures, thermostability, biocidal activity, and cell viability. The results show that changes in pH affect the structure and functionality of the keratin, suggesting its potential for biomedical applications.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Ramona Orlacchio, Simona Zuppolini, Iriczalli Cruz-Maya, Stefania Pragliola, Anna Borriello, Vincenzo Guarino, Rosalba Fittipaldi, Mariateresa Lettieri, Vincenzo Venditto
Summary: Poly-L-lactic acid (PLLA) aerogel-based scaffolds were prepared using a single step method of solvent extraction with supercritical CO2. The pore distribution and size of the aerogels were influenced by the solvent used and the crystalline forms present in the physical nodes. PLLA/CPO aerogels showed higher compressive strength, more homogeneous porosity, better biocompatibility, and cell viability than PLLA/BzOMe aerogels. Coating the aerogels with polydopamine (PDA) further improved the biocompatibility of PLLA/CPO aerogels.
Review
Chemistry, Analytical
William Serrano-Garcia, Irene Bonadies, Sylvia W. Thomas, Vincenzo Guarino
Summary: Fiber electronics manufactured by electrospinning have extensive applications in various fields, including batteries, sensors, energy storage, shielding, coatings, catalysts, drug delivery, tissue engineering, and smart textiles. Conductive-semiconductive polymers offer high surface area-to-volume ratios and electrical tunability, making them suitable for electronics, biofiltration, tissue engineering, biosensors, and green polymers. This review provides an overview of the processing of these polymers and their composites, emphasizing their electroactive properties and potential applications.
Article
Chemistry, Multidisciplinary
Nazirah Hamdan, Wan Khartini Wan Abdul Khodir, Shafida Abd Hamid, Mohd Hamzah Mohd Nasir, Ahmad Sazali Hamzah, Iriczalli Cruz-Maya, Vincenzo Guarino
Summary: In this study, polycaprolactone (PCL)/gelatin/graphene oxide (PGO) nanofibers were functionalized via plasma treatment with different monomeric groups. The functionalization influenced the morphology, wettability, mechanical properties, and thermal stability of the nanofibers. The results showed that PGO-M1 and PGO-M2 had good antibacterial activity, while PGO-M3 reduced the antibacterial properties compared to PGO nanofibers. The study highlights the importance of surface functionalization in engineering nanofibers with controlled antibacterial properties for various applications.
Article
Engineering, Biomedical
Ahmed Salama, Ahmed K. Saleh, Iriczalli Cruz-Maya, Vincenzo Guarino
Summary: A novel technique for preparing cationic bacterial cellulose hydrogel through in situ incorporation of cationic water-soluble cellulose derivative was proposed in this article. The cationic functionalization was deeply examined, and the results showed good cytocompatibility and antimicrobial properties of the cationic bacterial cellulose. This suggests a promising use of cationic hybrid materials in biomedical and bio-sustainable applications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Vincenzo Iannotti, Giovanni Ausanio, Anna M. Ferretti, Zaheer Ud Din Babar, Vincenzo Guarino, Luigi Ambrosio, Luciano Lanotte
Summary: This study used the electrospinning technique to produce polycarbonate urethane nanofibers incorporating magnetic particles. The aggregation and distribution of the magnetic nanoparticles within the nanofibers varied due to their different sizes. The magnetic response of the fiber mats was consistent with the morphology and polydispersity index of the embedded magnetic particles. By controlling the average size and polydispersity index of the magnetic particles, the magnetic properties can be modulated to design magneto-active systems for different applications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Iriczalli Cruz-Maya, Simona Zuppolini, Mauro Zarrelli, Elisabetta Mazzotta, Anna Borriello, Cosimino Malitesta, Vincenzo Guarino
Summary: In this study, core-shell microgels were produced by self-polymerization of dopamine and in situ precipitation of polydopamine onto alginate microbeads. The presence of polydopamine was confirmed by morphological and chemical analyses. The polydopamine coating improved the biomechanical properties of the microgel surfaces, leading to increased adhesion and cell proliferation, making core-shell microgels a promising model for studying in vitro cell interactions.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Engineering, Biomedical
Diego Omar Sanchez Ramirez, Claudia Vineis, Iriczalli Cruz-Maya, Cinzia Tonetti, Vincenzo Guarino, Alessio Varesano
Summary: Keratin is a biocompatible and biodegradable protein that can be extracted by sulphitolysis. It can be processed into nanofibers through electrospinning, which is an eco-friendly and economical method. Keratin nanofibers have great potential in biomedical applications due to their high specific surface area.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Chemistry, Physical
William Serrano-Garcia, Iriczalli Cruz-Maya, Anamaris Melendez-Zambrana, Idalia Ramos-Colon, Nicholas J. J. Pinto, Sylvia W. W. Thomas, Vincenzo Guarino
Summary: This study explores the morphology and in vitro response of electroconductive composite nanofibers for biomedical use. The composite nanofibers are prepared by blending piezoelectric polymer PVDF-TrFE with electroconductive materials such as CuO, P3HT, CuPc, and MB, resulting in unique combinations of electrical conductivity, biocompatibility, and other desirable properties. SEM analysis reveals differences in fiber size as a function of the electroconductive phase used, and electrical measurements show that MB has the highest charge transport ability. In vitro assays demonstrate a tunable response of fibers, with fibroblast cells showing a preferential interaction with P3HT-loaded fibers, making them suitable for biomedical applications.
Review
Pharmacology & Pharmacy
Carla Serri, Iriczalli Cruz-Maya, Irene Bonadies, Giovanna Rassu, Paolo Giunchedi, Elisabetta Gavini, Vincenzo Guarino
Summary: Significant advances in nanotechnologies have enabled the design of innovative devices suitable for biomedical applications, through increasing knowledge and refining of technical practices in green chemistry and bioengineering. New bio-sustainable methodologies are developing to fabricate drug delivery systems that combine properties of materials and bioactive molecules efficiently, meeting the demands of the health market. This work provides an overview of recent developments in bio-fabrication methods for designing green platforms, emphasizing their impact on biomedical and pharmaceutical applications.
Article
Biochemistry & Molecular Biology
Emad Tolba, Ahmed Salama, Ahmed K. Saleh, Iriczalli Cruz-Maya, Vincenzo Guarino
Summary: This study utilized polyelectrolytes to enhance the antibacterial properties of nanofibers and found that the CMID/SA functionalization positively affected the in vitro response of human mesenchymal stem cells (hMSCs) and had an impact on bacterial resistance.
Article
Chemistry, Analytical
Wan Khartini Wan Abdul Khodir, Mohamad Wafiuddin Ismail, Shafida Abd Hamid, Rusli Daik, Deny Susanti, Muhammad Taher, Vincenzo Guarino
Summary: Star-shaped copolymers can improve the interaction with drugs, resulting in significant benefits in drug administration and controlled release. In this study, two different star-shaped copolymers were synthesized and loaded with ciprofloxacin, with no significant differences observed in terms of drug entrapment efficiency and drug release. Both star-shaped copolymers also demonstrated good antibacterial properties and biocompatibility, making them promising for oral delivery applications.
Article
Polymer Science
Iriczalli Cruz-Maya, Rosaria Altobelli, Marco Antonio Alvarez-Perez, Vincenzo Guarino
Summary: There is growing interest in using micro-sized hydrogels to mimic cell niche structure and selected functionalities for tissue regeneration. In this study, bioactive composite microgels were optimized through electrohydrodynamic atomization (EHDA) for dentin-pulp complex regeneration. The mineralized microgels were successfully used to support in vitro osteogenesis, serving as an interesting model for studying dental tissue regeneration.
Article
Materials Science, Multidisciplinary
Zeynep Renkler, Iriczalli Cruz Maya, Vincenzo Guarino
Summary: The design of PVA-based multi-component membranes for tissue engineering is proposed in this study. The addition of polyethylene oxide (PEO) and gelatin improves the fiber processability and reduces the fiber size, while minimizing defect formation. Gelatin integration results in bioactive nanofibers with improved biological response. The processability of PVA fibers with conductive phases such as PVP and PEDOT:PSS has also been verified.
Article
Materials Science, Multidisciplinary
Roy Styles, Andrea Candini, Vincenzo Guarino, Islay Robertson, Priya Singh, Iriczalli Cruz-Maya, Valentina Benfenati, Amanda N. Abraham, Hiroshi Abe, Takeshi Ohshima, David A. Broadway, Andrew D. Greentree, Brant C. Gibson, Luigi Ambrosio, Jean-Philippe Tetienne, Philipp Reineck
Summary: Fluorescent nanodiamonds embedded in polyvinyl-alcohol fibers enable light-guiding-enabled optical quantum sensing, mitigating light-induced heating and paving the way for applications in biomedicine.
ADVANCED OPTICAL MATERIALS
(2023)