Article
Biochemistry & Molecular Biology
Pei-Lin Shao, Jiunn-Der Liao, Shun-Cheng Wu, Yu-Hsing Chen, Tak-Wah Wong
Summary: This study found that nonthermal microplasma (MP) treatment can promote the healing of skin wounds in diabetic mice, showing comparable or better efficacy than conventional negative pressure wound therapy (NPWT). The results suggest that MP treatment may be a promising approach for clinically treating diabetic wounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dermatology
Aref Rashed, Marton Csiszar, Agnes Beledi, Karoly Gombocz
Summary: Incisional negative pressure wound therapy (INPWT) has been shown to reduce wound infections, with a greater impact on scar formation during the proliferation phase rather than the inflammatory phase. The study did not find significant differences in inflammatory biomarkers between the INPWT group and the control group.
INTERNATIONAL WOUND JOURNAL
(2021)
Review
Oncology
Muhammad Faisal, Peter D. Berend, Rudolf Seemann, Stefan Janik, Stefan Grasl, Andrea Ritzengruber, Herbert Mendel, Arif Jamshed, Raza Hussain, Boban M. Erovic
Summary: NPWT is effective in treating complex wounds in head and neck cancer patients, with neck involvement being common. The study found that previous irradiation and diabetes mellitus have detrimental effects on wound healing after NPWT.
Article
Dermatology
Mengfan Wu, Dany Y. Matar, Zhen Yu, Ziyu Chen, Samuel Knoedler, Brian Ng, Oliver Darwish, Valentin Haug, Leigh Friedman, Dennis P. Orgill, Adriana C. Panayi
Summary: This study investigates the effect of mechanical force application on lymphangiogenesis in wound healing using a murine incisional wound model. The results show that negative pressure wound therapy can promote lymphatic vessel growth and drainage, leading to improved wound healing.
ADVANCES IN WOUND CARE
(2023)
Article
Pharmacology & Pharmacy
Benzion Amoyav, Yoel Goldstein, Eliana Steinberg, Ofra Benny
Summary: Microfluidics research has shown exponential growth in various applications, with the combination of 3D printing technology providing an efficient platform for particle production, reducing costs and time. The one-step fabrication process for a microfluidic chip for drug dissolution assays has advantages in terms of encapsulation efficiency and drug content compared to traditional batch formulations.
Article
Dermatology
Frank W. Brennfleck, Christine Bongards
Summary: Development of subcutaneous abdominal wound healing impairment (SAWHI) can greatly affect patient care, with delayed healing, increased risk of infection, and fascial dehiscence as potential complications. Negative pressure wound therapy in the out-of-hospital setting for the management of SAWHI has been found to be cost-effective, with lower cost of care and higher closure rates.
INTERNATIONAL WOUND JOURNAL
(2023)
Review
Dermatology
Guoqi Wang, Haiyan Xu, Gaoxiang Xu, Hao Zhang, Zhirui Li, Daohong Liu
Summary: Comparing negative pressure wound therapy with instillation (NPWTi) and standard negative pressure wound therapy, this study found that NPWTi resulted in fewer surgeries and dressing changes, and smaller wound areas post-treatment compared to NPWT. However, no significant differences were observed in other clinical outcomes between NPWTi and NPWT. Further well-designed RCTs with larger sample sizes are needed to validate these conclusions due to heterogeneity.
INTERNATIONAL WOUND JOURNAL
(2023)
Review
Polymer Science
Anthony Tony, Ildiko Badea, Chun Yang, Yuyi Liu, Garth Wells, Kemin Wang, Ruixue Yin, Hongbo Zhang, Wenjun Zhang
Summary: This paper provides a comprehensive review on the fabrication of PDMS microfluidic devices using additive manufacturing (AM) processes. Two approaches, direct printing and indirect printing, are classified. The focus of the study is on the printed mold approach, which involves casting PDMS materials with a printed mold. The paper also identifies knowledge gaps, proposes future work, and develops a novel classification of AM processes.
Article
Cell Biology
Jacob G. Hodge, Ashley L. Pistorio, Christopher A. Neal, Hongyan Dai, Jennifer G. Nelson-Brantley, Molly E. Steed, Richard A. Korentager, David S. Zamierowski, Adam J. Mellott
Summary: This study investigated the mechanisms by which NPWT modulates wound healing in a porcine wound model, revealing enhanced plasma protein adsorption and increased cellular colonization and tissue ingrowth. Genomic analysis showed significant changes in gene expression, while histological analysis demonstrated a foreign body response and encapsulation of GranuFoam particles in NPWT wounds.
WOUND REPAIR AND REGENERATION
(2022)
Article
Chemistry, Multidisciplinary
Junjie Huang, Hanxu Chen, Ning Li, Luoran Shang, Yan Bi, Yuanjin Zhao
Summary: In this paper, a novel herringbone microfluidic bioreactor with cell microcarriers integration was proposed for batch-preparation of stem cell exosomes. The microfluidic device provided a controllable fluidic environment for stem cells culturing on microcarriers and for exosomes secretion, leading to a high yield of exosomes. The exosomes harvested from the bioreactor showed excellent promoting effect on wound healing.
Article
Materials Science, Multidisciplinary
Masatoshi Maeki, Yuto Okada, Shuya Uno, Kaisei Sugiura, Yuichi Suzuki, Kento Okuda, Yusuke Sato, Masao Ando, Hiroyuki Yamazaki, Masaki Takeuchi, Akihiko Ishida, Hirofumi Tani, Hideyoshi Harashima, Manabu Tokeshi
Summary: Microfluidic devices are used in LNP-based vaccines and nanomedicine research, with a need for devices that are stiff enough for high flow rates and malleable for fabricating complex microchannel structures. This study reports the development of a glass-based microfluidic device for LNP production, comparable to PDMS-based devices in controlling LNP size. It also demonstrates a piling-and-numbering-up strategy for mass production of LNPs, with comparable performance to commercially available systems.
APPLIED MATERIALS TODAY
(2023)
Article
Dermatology
Xingan Qiu, Yifan Wu, Dong Zhang, Hao Zhang, Aixi Yu, Zonghuan Li
Summary: NPWT may promote wound healing by upregulating Raftlin and inhibiting oxidative stress levels, as shown by the increased expression of Raftlin, decreased levels of malondialdehyde, superoxide dismutase, and catalase, and accelerated wound healing time in the NPWT group compared to the control group.
CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY
(2021)
Article
Clinical Neurology
Benjamin M. Vidalis, Somtochukwu Ngwudike, Martin G. McCandless, Muhammad O. Chohan
Summary: This study reports on the use of incisional NPWT in patients undergoing surgery for MSD. The results suggest that the addition of incisional NPWT significantly reduces the occurrence of SSI and wound dehiscence in this vulnerable population.
WORLD NEUROSURGERY
(2022)
Article
Dermatology
Jianming Mo, Yuanjing Huang, Qiu Wang, Hua Zhong, Zhenwei Zhai, Yuechou Nong, Xiaodong Yan, Xiulu Huang, Jianhao Huang, Suping Yang, Jingxia Sun, Jiaxia Han, Xing Zhou, Wensheng Lu
Summary: This study explored the efficacy of autologous full-thickness skin graft combined with negative pressure wound therapy in treating refractory diabetic lower extremity ulcers. The results showed that the graft group had a larger ulcer area, but a shorter time to complete wound re-epithelialization and faster healing speed. Additionally, the total treatment cost in the graft group was lower.
INTERNATIONAL WOUND JOURNAL
(2023)
Review
Health Care Sciences & Services
Alvaro Astasio-Picado, Maria Dolores Murcia Montero, Miriam Lopez-Sanchez, Jesus Jurado-Palomo, Paula Cobos-Moreno, Beatriz Gomez-Martin
Summary: This review analyzed the effectiveness of negative pressure therapy in complex wounds, compared its effectiveness with other conventional treatments and their combination, and evaluated the quality of life and main characteristics of patients undergoing negative pressure therapy. The results showed that negative pressure therapy has the advantages of rapid rate of granulation, prevention and effective treatment of infections, variety and malleability of dressings, various applications, and the possibility of using it with other therapies to accelerate wound closure.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Dermatology
David G. Armstrong, Robert D. Galiano, Dennis P. Orgill, Paul M. Glat, Marissa J. Carter, Lawrence A. Di Domenico, Alexander M. Reyzelman, Charles M. Zelen
Summary: This study compared the use of cryopreserved bioactive split thickness skin allografts with standard care in the treatment of non-healing diabetic foot ulcers. The results showed that the use of the allografts significantly improved wound healing and reduction in wound area compared to standard care, without increasing the incidence of adverse events.
INTERNATIONAL WOUND JOURNAL
(2022)
Article
Dermatology
David G. Armstrong, Dennis P. Orgill, Robert D. Galiano, Paul M. Glat, Jarrod P. Kaufman, Marissa J. Carter, Lawrence A. DiDomenico, Charles M. Zelen
Summary: Diabetic foot infections continue to be a major challenge for health care systems. A study comparing a novel PRBM treatment with a standard of care (SOC) for chronic DFUs showed that PRBM resulted in a higher percentage of wounds closed, faster healing time, and greater reduction in wound area. The use of PRBM was safe and effective.
INTERNATIONAL WOUND JOURNAL
(2022)
Article
Dermatology
Adriana C. Panayi, Dany Y. Matar, Valentin Haug, Mengfan Wu, Dennis P. Orgill
Summary: This study conducted a retrospective analysis of a surgical database to investigate the surgical management of patients with hidradenitis suppurativa (HS). The results showed that surgical management for HS is associated with low postoperative complications, making surgery a viable treatment option for this debilitating disease.
ADVANCES IN WOUND CARE
(2023)
Review
Surgery
Xinxin Liu, Ayushi Aggarwal, Mengan Wu, Oliver A. Darwish, Kodi Baldino, Valentin Haug, Riaz A. Agha, Dennis P. Orgill, Adriana C. Panayi
Summary: Diabetes mellitus poses a higher risk of complications in patients undergoing breast reconstruction after mastectomy, including surgical complications, infection, and tissue necrosis.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2022)
Article
Dermatology
Mengfan Wu, Zhen Yu, Dany Y. Matar, Mehran Karvar, Ziyu Chen, Brian Ng, Shimpo Aoki, Valentin Haug, Dennis P. Orgill, Adriana C. Panayi
Summary: This study investigated the effect of human amniotic membrane (AM) on chronic wound healing using an oxidative stress murine model. The results showed that AM treatment increased vascular density and maturity, promoted leukocyte infiltration and growth factor delivery, and reduced cell death. These findings provide new insights into the mechanisms of action of human AM in chronic wound healing.
ADVANCES IN WOUND CARE
(2023)
Article
Dermatology
Dany Y. Matar, Brian Ng, Oliver Darwish, Mengfan Wu, Dennis P. Orgill, Adriana C. Panayi
Summary: The skin is an important barrier against foreign pathogens and its compromise can result in skin conditions and chronic wounds. Inflammatory skin conditions severely affect patients' quality of life and have a significant economic impact on the healthcare system. Recent advances in understanding skin inflammation include the development of new pharmaceuticals and advancements in clinical applications of mechanical forces and bioscaffolds.
ADVANCES IN WOUND CARE
(2023)
Article
Engineering, Biomedical
Mengfan Wu, Dany Y. Matar, Zhen Yu, Mehran Karvar, Ziyu Chen, Brian Ng, Samuel Knoedler, Oliver Darwish, Shailesh Agarwal, Dennis P. Orgill, Adriana C. Panayi
Summary: The study demonstrated that external volume expansion combined with xenogenic implantation of human allograft adipose matrix can improve fat graft survival, with pre- and post-conditioning playing a significant role in adipose tissue survival.
BIOMEDICAL MATERIALS
(2022)
Article
Dermatology
David G. Armstrong, Dennis P. Orgill, Paul M. Glat, Robert D. Galiano, Zachary L. Rasor, Adam Isaac, Marissa Carter, Charles M. Zelen
Summary: This study evaluated the safety and efficacy of a device designed to offload diabetic foot ulcers by improving circulation and monitoring patient compliance. The device successfully accelerated wound healing and improved pain without any adverse events reported.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Surgery
Dennis P. Orgill, Amy K. McNulty
Summary: Vacuum Assisted Closure (VAC) has revolutionized the treatment of complex and chronic wounds by directly applying mechanical forces to the wound surface. This interaction between the VAC device and the wound causes deformation and stretching of cells, while also promoting fluid removal from the tissues. The use of VAC devices has been shown to enhance angiogenesis and improve vessel morphology compared to standard dressings, with factors such as foam pore size, pressure, and waveform playing a role in the therapeutic effect.
SURGICAL INNOVATION
(2023)
Article
Dermatology
Mengfan Wu, Dany Y. Matar, Zhen Yu, Ziyu Chen, Samuel Knoedler, Brian Ng, Oliver Darwish, Valentin Haug, Leigh Friedman, Dennis P. Orgill, Adriana C. Panayi
Summary: This study investigates the effect of mechanical force application on lymphangiogenesis in wound healing using a murine incisional wound model. The results show that negative pressure wound therapy can promote lymphatic vessel growth and drainage, leading to improved wound healing.
ADVANCES IN WOUND CARE
(2023)
Article
Microbiology
Felix Ellett, Nazli I. Kacamak, Carla R. R. Alvarez, Eduardo H. S. Oliveira, Hatice Hasturk, Bruce J. J. Paster, Alpdogan Kantarci, Daniel Irimia
Summary: Recent studies have found that Fusobacterium nucleatum (Fn), a common bacterium in the oral cavity, is associated with various systemic diseases. The mechanisms behind this association are still unclear. In this study, we use advanced technology to investigate the interactions between Fn and neutrophils. Our findings show that Fn can survive within human neutrophils and be transported over long distances by these cells. We validate these observations in vivo using a zebrafish model. These results support the hypothesis that bacterial dissemination by neutrophils may be a link between oral and systemic diseases, and could potentially lead to new therapeutic approaches.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Editorial Material
Medicine, General & Internal
Harriet Kiwanuka, Alice T. Wang, Dennis P. Orgill
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Surgery
Saiuj Bhat, Benjamin Chia, Ian P. Barry, Adriana C. Panayi, Dennis P. Orgill
Summary: This article is a systematic review and meta-analysis of free tissue transfer for the treatment of diabetic foot ulcers. The results suggest that free tissue transfer may be an effective treatment modality for recalcitrant diabetic foot ulcers, but the overall certainty of evidence is very low. Future studies should focus on long-term functional outcomes and the development of patient selection algorithms.
EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
(2023)
Article
Dermatology
David G. Armstrong, Dennis P. Orgill, Robert Galiano, Paul M. Glat, Lawrence Didomenico, Nikolai A. Sopko, Edward W. Swanson, Felix Sigal, Robert Snyder, William W. W. Li, Marissa Carter, Charles M. Zelen
Summary: A novel autologous heterogeneous skin construct (AHSC) has been shown to be effective in facilitating complete healing of Wagner 1 diabetic foot ulcers compared to standard of care (SOC) treatment. The final analysis of 100 patients further supports the findings from the interim analysis, with significantly more diabetic wounds closed in the AHSC treatment group compared to the SOC control group. The AHSC treatment also demonstrated a significant difference in percentage area reduction between groups over 8 weeks.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Biochemical Research Methods
Felix Ellett, Daniel Irimia
Summary: Neutrophils are the first responders to inflammation and infection, making their behavior a valuable biomarker for antimicrobial defenses. Traditional methods for studying neutrophils require isolating them from blood, which is time-consuming and expensive. Microfluidic technologies have been developed to analyze neutrophils directly in blood, but red blood cell contamination remains an issue. In this study, a new strategy using microscale passive redirection filters (PRFs) is presented to reduce red blood cell contamination and improve the accuracy of neutrophil assays.