Article
Chemistry, Multidisciplinary
Da Chen, Ziyi Song, Meiling Lian, Yi Yang, Shen Lin, Lehui Xiao
Summary: A new color-coded single-particle detection method using platelet membrane-coated fluorescent polystyrene nanoparticles as probes has been demonstrated for sensitive fibrinogen detection. The proposed biosensor shows a linear range of 30-300 μg mL(-1) and a limit of detection of 3.9 μg mL(-1) for fibrinogen detection, with the ability to selectively detect fibrinogen in real sample applications.
Article
Biology
Albertus Viljoen, Felipe Viela, Marion Mathelie-Guinlet, Dominique Missiakas, Giampiero Pietrocola, Pietro Speziale, Yves F. Dufrene
Summary: Through force spectroscopy studies on living bacteria, Viljoen et al. characterise the binding of S. aureus to host von Willebrand factor (vWF). They propose that S. aureus vWF-binding protein triggers an ultra-strong interaction between the adhesin clumping factor A and vWF.
COMMUNICATIONS BIOLOGY
(2021)
Article
Hematology
Zixiang Leonardo Liu, Christopher Bresette, Cyrus K. Aidun, David N. Ku
Summary: This study elucidates the biophysics of shear-induced platelet aggregation (SIPA) using a multiscale in silico model and a thrombosis-on-a-chip device. The findings show that SIPA begins with the elongation of von Willebrand factor (VWF), followed by agglomeration of platelets in the flow mediated by soluble VWF entanglement, and eventually captured by immobilized VWF. Increasing soluble VWF concentration leads to higher SIPA rates, and the morphology of mural aggregates is controlled by VWF molecular weight.
Article
Multidisciplinary Sciences
Amin Polzin, Lisa Dannenberg, Rene M'Pembele, Philipp Mourikis, David Naguib, Saif Zako, Carolin Helten, Tobias Petzold, Bodo Levkau, Thomas Hohlfeld, Mareike Barth, Tobias Zeus, Stephan Sixt, Ragnar Huhn, Payam Akhyari, Artur Lichtenberg, Malte Kelm, Till Hoffmann
Summary: In patients with infective endocarditis, platelet reactivity is increased in those with Staphylococcus aureus (SA) infection. However, the use of aspirin can reduce this increased reactivity without increasing bleeding risk. More research is needed to confirm the potential benefits of aspirin in SA-positive infective endocarditis.
SCIENTIFIC REPORTS
(2022)
Article
Biophysics
Mansur Zhussupbekov, Rodrigo Mendez Rojano, Wei-Tao Wu, James F. Antaki
Summary: This article introduces a new multi-constituent model of thrombosis, providing a new perspective on thrombus formation by studying the conformational changes of vWF in complex flow fields. Through model validation, it is proven that the simulation results are in good agreement with experimental observations, further validating the effectiveness of this model.
BIOPHYSICAL JOURNAL
(2022)
Article
Engineering, Biomedical
Maja Struczynska, Izabela Firkowska-Boden, Nathan Levandovsky, Reinhard Henschler, Nour Kassir, Klaus D. Jandt
Summary: Control of protein adsorption is crucial for successful integration of healthcare materials in the body. Human plasma fibrinogen (HPF) is a key regulator for platelet behavior and pathological clot formation at the blood-material interface. This study explores the role of rutile crystallographic orientation on HPF conformation and its effect on platelet adhesion and activation. The findings suggest that specific rutile facets can modulate platelet behavior through HPF conformation, potentially reducing the risk of clot formation and thromboembolic complications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Applied
Katerina Lehka, Jana Starigazdova, Jiri Mrazek, Kristina Nesporova, Matej Simek, Vojtech Pavlik, Josef Chmelar, Martin Cepa, Martin Eugenio Barrios-Llerena, Anna Kocurkova, Eva Krivakova, Ludmila Koukalova, Lukas Kubala, Vladimir Velebny
Summary: The foreign body response (FBR) is a series of reactions that occur after the implantation of biomaterials, leading to the formation of a fibrotic capsule and health complications. The severity of the FBR is influenced by the physicochemical characteristics of the implant, implantation method, and immune system activation. In this study, we present an in vitro model that evaluates protein sorption and cell adhesion on implanted materials to assess their potential to induce FBR in the peritoneum. Our model demonstrated that fibrinogen plays a crucial role in driving cell adhesion, while surface hydrophobicity/hydrophilicity and quantity of adsorbed proteins were not determining factors. The dissolution rate of the material was found to be a crucial factor in inducing long-term cell adhesion and FBR. This model helps predict the probability of FBR without the need for in vivo animal testing.
CARBOHYDRATE POLYMERS
(2023)
Article
Medical Laboratory Technology
Zufar A. Gabbasov, Yuliya N. Avtaeva, Ivan S. Melnikov, Sergey D. Okhota, Martin Caprnda, Ioana Mozos, Robert Prosecky, Luis Rodrigo, Peter Kruzliak, Nadezhda Zozulya
Summary: This study aimed to test a novel method for assessing platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. The results showed that platelet adhesion intensity was significantly higher in whole blood compared to platelet-rich plasma, and inhibiting different receptors could affect platelet adhesion. The system effectively registers platelet adhesion under controlled-flow conditions, which may be useful for studying platelet adhesion kinetics.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Hematology
Yana Roka-Moiia, Samuel Miller-Gutierrez, Daniel E. Palomares, Joseph E. Italiano, Jawaad Sheriff, Danny Bluestein, Marvin J. Slepian
Summary: Our study shows that exposure of platelets to elevated shear stress leads to downregulation of GPIb and alpha(IIb)beta(3) receptors on platelets, while increasing the generation of platelet-derived microparticles expressing elevated levels of these receptors. This mechanism limits platelet activation and aggregatory response induced by physiological stimuli.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Hematology
Cihan Ay, Ingrid Pabinger, Katarina D. Kovacevic, Georg Gelbenegger, Christian Schorgenhofer, Peter Quehenberger, Petra Jilma-Stohlawetz, Raute Sunder-Plassman, James C. Gilbert, Shuhao Zhu, Bernd Jilma, Ulla Derhaschnig
Summary: The potential benefit of Rondoraptivon pegol in patients with type 2B von Willebrand disease was studied. The results showed that the drug rapidly increased platelet counts and VWF/FVIII levels, and corrected the underlying defect caused by thrombocytopenia. This provides a new potential option for the prophylaxis and treatment of type 2B von Willebrand disease.
Review
Biochemistry & Molecular Biology
Jinmi Zou, Frauke Swieringa, Bas de Laat, Philip G. de Groot, Mark Roest, Johan W. M. Heemskerk
Summary: This paper reviews the functional reversibility of integrin alpha IIb beta 3 activation and transient aggregation and discusses the signaling pathways involved. The study finds that ADP P2Y(1) and P2Y(12) receptor signaling via phosphoinositide 3-kinases and Akt are the principal pathways linked to reversible integrin activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Jordan Thomas Lovegrove, Radhika Raveendran, Patrick Spicer, Stephan Foerster, Christopher J. Garvey, Martina H. Stenzel
Summary: Margination refers to the movement of particles towards the endothelial wall within blood vessels. This study focused on 2D platelet particles as a potential drug delivery system and quantified their margination propensity. The results showed that 2D platelet particles exhibited increased margination compared to spherical particles, with higher margination observed at higher shear rates.
Article
Medicine, General & Internal
Horia Bumbea, Ana Maria Vladareanu, Ion Dumitru, Viola Maria Popov, Cristina Ciufu, Anca Nicolescu, Minodora Onisai, Cristina Marinescu, Diana Cisleanu, Irina Voican, Sinziana Sarghi
Summary: This study found that patients with acute myeloid leukemia (AML) have impaired platelet function, characterized by changes in adhesion receptors and activation markers. Flow cytometry can effectively identify multiple functional platelet impairments in AML pathogenesis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Polymer Science
Xiaohong Liu, Mohammad-Amin Moradi, Tom Bus, Johan P. A. Heuts, Michael G. Debije, Albert P. H. J. Schenning
Summary: Monodisperse, micrometer-sized liquid crystalline shells are prepared using seeded dispersion polymerization, allowing for programmable alignment and shape control. Different alignment of liquid crystal in the polymer shells is achieved by using different polymer seeds, resulting in various shapes of LC polymer shells after seed removal. The collapse of the shells at defect points is observed, indicating the versatility of the method in preparing biconcave LC polymer shells.
Article
Biochemistry & Molecular Biology
Magdalena Boncler
Summary: A commercial nonbinding surface effectively prevents protein adsorption, but its effect on platelet adhesion and adsorption of plasma/ECM proteins is not well defined. This study compared the nonbinding surface to nontreated and high-binding surfaces, evaluating platelet adhesion and protein adsorption. The nonbinding surface effectively prevented platelet adsorption by 61-93% and reduced platelet adhesion by 92% when not coated with any protein. However, it did not prevent platelet adhesion to fibrinogen, indicating it is more of a low-fouling material than a nonfouling one.
MACROMOLECULAR BIOSCIENCE
(2023)