期刊
JOURNAL OF BIOMECHANICS
卷 47, 期 9, 页码 2197-2204出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2013.11.017
关键词
MSC; Atomic force microscopy; Bone sialoprotein; Cell stiffness; Osteoblast differentiation; Osteocalcin
资金
- NCRR of the NIH [P40RR017447]
- National Science Foundation [CBET-0932510]
- NSF Stem Cell Biomanufacturing IGERT
- President's Undergraduate Research Award at Georgia Tech
Although it has been established that cellular stiffness can change as a stem cell differentiates, the precise relationship between cell mechanics and other phenotypic properties remains unclear. Inherent cell heterogeneity and asynchronous differentiation complicate population analysis; therefore, single-cell analysis was employed to determine how changes in cell stiffness correlate with changes in molecular biomarkers during differentiation. Design of a custom gridded tissue culture dish facilitated single-cell comparisons between cell mechanics and other differentiation biomarkers by enabling sequential measurement of cell mechanics and protein biomarker expression at the single cell level. The Young's modulus of mesenchymal stem cells was shown not only to decrease during chemically-induced osteoblast differentiation, but also to correlate more closely with the day of differentiation than did the relative expression of the traditional osteoblast differentiation markers, bone sialoprotein and osteocalcin. Therefore, cell stiffness, a measurable property of individual cells, may serve as an improved indicator of single-cell osteoblast differentiation compared to traditional biological markers. Revelation of additional osteoblast differentiation indicators, such as cell stiffness, can improve identification and collection of starting cell populations, with applications to mesenchymal stem cell therapies and stem cell-based tissue engineering. (C) 2013 Elsevier Ltd. All rights reserved.
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