期刊
JOURNAL OF BIOMECHANICS
卷 42, 期 14, 页码 2336-2340出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2009.06.026
关键词
Skeletal muscle fibers; Force depression; BDM; Cross-bridge theory
资金
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program for Molecular and Cellular Biomechanics
- Canadian Institutes of Health Research
The steady-state isometric force following active muscle shortening is smaller than the corresponding force obtained for purely isometric contractions. This so-called residual force depression has been observed consistently for more than half a century, however its mechanism remains a matter of scientific debate. [Marechal, G., Plaghki, L, 1979. The deficit of the isometric tetanic tension redeveloped after a release of frog muscle at a constant velocity. J Gen Physiol. 73, 453-467] suggested that force depression might be caused by alterations in the cross-bridge kinetics following muscle shortening, but there is no research studying force depression systematically for altered cross-bridge kinetic conditions. The purpose of this study was to investigate if force depression affects so-called weakly and strongly bound cross-bridges to the same degree. In order to achieve this aim, we modified the ratio of weakly to strongly bound cross-bridges with 2,3-butanedione monoxime (BDM) in single frog fibers. BDM inhibits the formation of strongly bound cross-bridges in a dose-dependent manner, thus the ratio of weakly to strongly bound cross-bridges could be altered in a systematic way. We found that the absolute amount of force depression was decreased by 50% while the relative amount was decreased by 12% in BDM exposed fibers compared to fibers in normal Ringer's solution. Furthermore, force depression was accompanied by a decrease in stiffness that was much greater in normal compared to BDM exposed fibers. leading to the conclusion that force depression was caused by an inhibition of cross-bridge attachment following fiber shortening and that this inhibition primarily affected cross-bridges in the strongly bound states. (C) 2009 Elsevier Ltd. All rights reserved.
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