Article
Chemistry, Analytical
Bryan Ang, Ruhollah Habibi, Ciaren Kett, Wai Hoe Chin, Kellie L. Tuck, Adrian Neild, Victor J. Cadarso
Summary: Microfluidic concentration technology can detect low concentrations of bacteria in samples, making it suitable for time-sensitive situations such as clinical settings and food quality control. By using a packed bed of micro-particles activated by surface acoustic waves, bacteria can be efficiently captured and recovered for further analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Suhyeon Kim, Hyoryung Nam, Eunji Lee, Ji-Won Huh, You-Me Kim, Hyungkook Jeon, Geunbae Lim
Summary: We have developed a multifunctional microfluidic device that can lyse cells and separate intracellular components, offering advantages of simple fabrication, low cost, and bubble-free operation. The device utilizes an amplified electric field in the ion-depletion region formed by ion concentration polarization, allowing for sequential processing of cell lysis and separation of nano-sized components.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Chang Chen, Panpan Li, Tianruo Guo, Siyuan Chen, Dong Xu, Huaying Chen
Summary: This study developed a microfluidic device that allows dynamic variations of concentration in a channel and concentration distribution in multiple channels by adjusting the flow resistance using programmable pneumatic microvalves. The device offers superior application potentials in analytical chemistry, drug screening, and cell biology research.
Article
Environmental Sciences
Lang Zhou, Reinaldo E. Alcalde, Jinzi Deng, Baltazar Zuniga, Robert A. Sanford, Bruce W. Fouke, Charles J. Werth
Summary: This study found that Shewanella oneidensis is able to reduce nitrate through chemotactic migration, but only in regions with lower bactericidal concentrations. Migration is inhibited in regions with higher bactericidal concentrations of antibiotics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Vinit Kumar Yadav, Preetha Ganguly, Prashant Mishra, Samaresh Das, Dhiman Mallick
Summary: This study developed a versatile magnetic microfluidic device for concentration-dependent compartmentalization of a magnetically controlled anticancer drug. By optimizing the microfluidic design and magnet configuration, predictable concentration control and gradient generation were achieved. The results of drug screening and cytotoxicity testing using the device were consistent with traditional methods.
Article
Biochemical Research Methods
Bryan Ang, Ankush Sookram, Citsabehsan Devendran, Vincent He, Kellie Tuck, Victor Cadarso, Adrian Neild
Summary: Surface acoustic wave (SAW) driven devices often use polymeric microfluidic channels with low acoustic impedance mismatch to the fluid in contact for precise control of the wave field. This work demonstrates the successful implementation of integrating a glass insert at the ceiling of the PDMS microfluidic channel in a SAW activated nanosieve, resulting in a significant increase in flow rate and maintenance of high capture efficiencies. The glass-inserted device allows for processing larger volume samples, overcoming a main limitation of these devices.
Article
Chemistry, Analytical
Joana Carvalho, Lorena Dieguez, Andrey Ipatov, Joana Rafaela Guerreiro, Alejandro Garrido-Maestu, Sarah Azinheiro, Marta Prado
Summary: Purification and concentration of DNA is crucial for DNA-based analysis. The novel miniaturized disposable device showed high efficiency in DNA purification, including handling highly diluted samples and environmental DNA with great potential.
Article
Chemistry, Analytical
Shizheng Zhou, Edgar S. Fu, Bingbing Chen, Hong Yan
Summary: This study introduces a unique microfluidic coral polyp culture chip, which allows for multivariable analysis and has been verified through numerical simulation.
Review
Cell Biology
Nicole M. E. Valle, Mariana P. Nucci, Arielly H. Alves, Luiz D. Rodrigues, Javier B. Mamani, Fernando A. Oliveira, Caique S. Lopes, Alexandre T. Lopes, Marcelo N. P. Carreno, Lionel F. Gamarra
Summary: This systematic review analyzed the development and functionality of microfluidic concentration gradient generators (CGGs) for toxicological evaluation. The majority of articles focused on the fabrication methods, characteristics of the CGGs, biological models, and evaluation techniques. Soft lithography using PDMS material and the Christmas tree CGG design were popular choices. Imaging and microscopy techniques were commonly used for evaluating the toxic effects. CGGs showed advantages over other techniques and were applicable to various biological models.
Article
Chemistry, Analytical
Paulo Henrique Maciel Buzzetti, Maiara Mitiko Taniguchi, Nayara de Souza Mendes, Renata Correa Vicentino, Jean Halison de Oliveira, Bento Pereira Cabral Junior, Marcos de Souza, Johny Paulo Monteiro, Emerson Marcelo Girotto
Summary: In this study, a microfluidic device based on serial dilution was developed to generate nonlinear concentration gradients for dyes and biomolecules. The device was mathematically described and statistically validated, showing reproducibility in experiments and agreement with computational fluid dynamics simulation. The applicability of the device was demonstrated by coupling it with a surface plasmon resonance (SPR) biosensor, and the results were validated by fluorescence emissions.
Article
Biochemical Research Methods
Shang-Jyun Lin, Po-Hsuan Chao, Ho-Wen Cheng, Juen-Kai Wang, Yuh-Lin Wang, Yin-Yi Han, Nien-Tsu Huang
Summary: This paper presents a microfluidic device that generates a concentration gradient for antibiotics in order to perform antibiotic treatment on bacteria. The system simplifies and speeds up the process of antimicrobial susceptibility testing.
Article
Chemistry, Analytical
Wei Wang, Haleigh M. Grace, Paul A. Flowers
Summary: This study describes a simple microfluidic device that allows spectroelectrochemical measurements on submicroliter samples. By utilizing embedded electrode chips and a solution-cast ionomeric film, measurements on smaller sample volumes can be achieved.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Physical
Xugen Chen, Likai Hou, Zhongqiang Zhang, Renyong Lin, Rong Lin, Chenggang Yan, Fubing Bao
Summary: Concentration gradient plays a crucial role in comparative analysis in the fields of biomedicine and chemistry. This study presents a simple microfluidic device that allows the encapsulation of soluble reagents with large-scale concentration gradients in nanoliter-scale water-in-oil droplets for comparative analysis. The concentration gradients can be easily adjusted by changing the flow rates of the coaxial channels. With this method, nanoliter-grade reagents can be prepared, reactions can be conducted, and analyses can be performed with higher concentration gradients and less sample consumption.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Engineering, Chemical
Yice Shao, Hong Wang, Qian Zhang, Zhenting Xie, Xun Zhu, Yudong Ding, Rong Chen, Qiang Liao
Summary: We propose a cross microchannel structure based on electroosmotic drive for accurate and fast control of fluid flow velocity and mixing. Experimental and numerical investigations are conducted to study the effects of channel width, applied voltage, and waveform on fluid mixing and flow behavior. It is found that the flow velocity and fluid mixing ratio can be regulated efficiently by the applied voltage. The control accuracy of the fluid mixing ratio can be significantly improved by reducing the width of one of the microchannels. Various concentration and velocity waveforms can be generated by controlling the voltage waveform. Our findings provide important insights for the design of electrically operated fluid devices.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Peng Huo, Bingrui Xu, Zhibo Gu, Mingzhuo Su, Shmuel M. Rubinstein, Daosheng Deng
Summary: This study systematically investigated the effects of supporting electrolyte on inert cations in multi-component electrolytes, revealing a new phenomenon of remote electroconvection. The visualization of the correlation between concentration valley and electroconvection was discussed, along with the underlying physical mechanisms. The study has implications for future research on multi-component electrolytes in water treatment and battery energy storage.
Article
Cell Biology
Sean McCutcheon, Uchenna Unachukwu, Ankush Thakur, Robert Majeska, Stephen Redenti, Maribel Vazquez
CELL ADHESION & MIGRATION
(2017)
Article
Ophthalmology
Juan Pena, Nihan Dulger, Tanya Singh, Jing Zhou, Robert Majeska, Stephen Redenti, Maribel Vazquez
EXPERIMENTAL EYE RESEARCH
(2018)
Article
Cell & Tissue Engineering
Ankush Thakur, Shawn Mishra, Juan Pena, Jing Zhou, Stephen Redenti, Robert Majeska, Maribel Vazquez
JOURNAL OF TISSUE ENGINEERING
(2018)
Editorial Material
Ophthalmology
Juan S. Pena, Maribel Vazquez
Article
Chemistry, Analytical
Juan S. Pena, Denise Robles, Stephanie Zhang, Maribel Vazquez
Article
Ophthalmology
Shawn Mishra, Juan S. Pena, Stephen Redenti, Maribel Vazquez
EXPERIMENTAL EYE RESEARCH
(2019)
Article
Cell Biology
Caroline D. Pena, Stephanie Zhang, Robert Majeska, Tadmiri Venkatesh, Maribel Vazquez
Article
Cell & Tissue Engineering
Tanya Singh, Denise Robles, Maribel Vazquez
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Neurosciences
Juan S. Pena, Maribel Vazquez
Review
Chemistry, Analytical
Maribel Vazquez
Article
Chemistry, Analytical
Stephen Ryan Mut, Shawn Mishra, Maribel Vazquez
Summary: Millions of adults worldwide are affected by progressive vision loss. Current cell replacement therapies face a challenge of inability to migrate to targeted areas. A bioengineering system called mu-Eye has been developed to stimulate and examine the migration of retinal stem cells (SCs) within eye facsimiles using external stimuli, showing promising results for promoting migration-targeted strategies for retinal cell replacement therapy.
Article
Engineering, Biomedical
Tanya Singh, Maribel Vazquez
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2019)
Meeting Abstract
Endocrinology & Metabolism
Sean Mccutcheon, Robert Majeska, David Spray, Maribel Vazquez, Mitchell Schaffler
JOURNAL OF BONE AND MINERAL RESEARCH
(2018)
Article
Chemistry, Analytical
Shawn Mishra, Maribel Vazquez
Article
Engineering, Biomedical
Sean McCutcheon, Robert Majeska, Mitchell Schaffler, Maribel Vazquez
BIOMEDICAL MICRODEVICES
(2017)