Article
Biochemistry & Molecular Biology
Yuhan Xia, Aoi Ikedo, Ji-Won Lee, Tadahiro Iimura, Kazuki Inoue, Yuuki Imai
Summary: Our study reveals that Utx positively regulates osteoblast-to-osteocyte differentiation through H3K27me3 modifications in osteocyte genes. This provides new insight into the molecular mechanism of osteocyte differentiation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Austin P. Hensley, Audrey McAlinden
Summary: miRNAs are important epigenetic factors in regulating bone development, homeostasis, and repair processes by suppressing specific target genes. Studies have shown that alterations in miRNA levels or activity can lead to significant changes in cellular processes. Research has also revealed the potential therapeutic applications of miRNA regulation in various bone conditions.
Article
Biotechnology & Applied Microbiology
Xia Li, Ruisheng Xu, Jun-Xing Ye, Feng-Lai Yuan
Summary: Oral bisphosphonates (BPs) are commonly used for the treatment of osteoporosis. This study found that long-term BP treatment can modulate the expression of specific microRNAs (miRNAs), specifically miR-30a-5p, which is negatively correlated with bone formation. In vitro and in vivo experiments demonstrated that miR-30a-5p inhibits osteoblastic differentiation and bone formation. This research provides important insights into the regulatory mechanisms of long-term BP treatment in postmenopausal osteoporosis.
Review
Biochemistry & Molecular Biology
S. Pranav Adithya, K. Balagangadharan, N. Selvamurugan
Summary: In bone biology, epigenetics and histone modifications play a crucial role in the commitment and differentiation of mesenchymal stem cells towards osteoblasts. Understanding the importance of histone modifications in osteogenesis can lead to the development of epigenetic-based therapies for genetic bone disorders. This review highlights the significance of epigenetic modifications, including post-translational modifications of histones, in regulating gene expression during osteoblast differentiation and discusses future advancements in the field of epigenetics for orthopaedic therapeutics.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2022)
Review
Genetics & Heredity
Jinkun Xia, Wangsen Cao
Summary: Studies show that epigenetic modifications affect the Klotho gene in renal diseases, impacting disease progression. Lowered Klotho levels worsen the condition, but beneficial changes can be made through epigenetic interventions.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Genetics & Heredity
Parisa Dashti, Jeroen van de Peppel, Roman Thaler, Christopher R. Paradise, Gary S. Stein, Martin A. Montecino, Johannes P. T. M. van Leeuwen, Bram J. van der Eerden, Amel Dudakovic, Andre J. van Wijnen
Summary: This study found that Smyd2 inhibits proliferation and mineral deposition by osteoblasts, and operates parallel to the suppressive effects of Ezh2 and H3K27 trimethylation on osteoblast differentiation.
Review
Biochemistry & Molecular Biology
Wilson Cheuk Wing Chan, Zhijia Tan, Michael Kai Tsun To, Danny Chan
Summary: Bone is a dynamic tissue that responds to environmental changes such as nutrition and mechanical stress. Bone homeostasis is maintained through bone remodeling in adulthood, which involves a controlled balance between bone-resorbing osteoclasts and bone-forming osteoblasts. During embryonic development, bones can form through intramembraneous or endochondral ossification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Cheng Wang, Peng Wang, Feng Li, Yang Li, Minwei Zhao, Hui Feng, Haoye Meng, Junyang Li, Peng Shi, Jiang Peng, Hua Tian
Summary: This study investigated the expression of miRNA-214 in human osteoporotic bone tissue and tested the utility of AAV expressing a miRNA-214 inhibitor in preventing local osteoporosis in a rat model. The results showed that miRNA-214 expression was significantly increased in osteoporotic bone tissue. Moreover, AAV-anti-miRNA-214 promoted osteoblast activity and inhibited osteoclast activity in the femoral condyles of osteoporotic rats, improving bone metabolism and slowing the progression of osteoporosis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Rogerio S. Ferreira, Rodrigo A. da Silva, Georgia Da S. Feltran, Ericka Patricia da Silva, Rahyza I. F. de Assis, Emanuel Silva Rovai, Willian F. Zambuzzi, Denise C. Andia
Summary: The results of this study demonstrate the crucial role of JARID1B in repressing the osteogenic phenotype of PDLCs, suggesting its potential as a therapeutic target in pre-clinical protocols.
Review
Chemistry, Medicinal
Carmen Nunez-Carro, Margarita Blanco-Blanco, Karla Mariuxi Villagran-Andrade, Francisco J. Blanco, Maria C. de Andres
Summary: In this study, we reviewed the current understanding of epigenetic mechanisms in relation to the pathogenesis of OA, particularly in articular cartilage. Evidence suggests that dysregulation of crucial cartilage molecules is a result of aberrant epigenetic regulatory mechanisms, which contribute to the development and progression of OA. This provides an opportunity to explore new therapeutic targets and novel biomarkers for the disease.
Article
Endocrinology & Metabolism
Linlin Zhang, Kecheng Zhu, Jingzun Xu, Xiaojing Chen, Chunxiang Sheng, Deng Zhang, Yuying Yang, Lihao Sun, Hongyan Zhao, Xiao Wang, Bei Tao, Libin Zhou, Jianmin Liu
Summary: CBP and p300, as important histone acetyltransferases, have been found to affect bone density and strength. The study further demonstrates that CBP/p300 HAT regulates osteogenic gene expression by modulating transcription of beta-catenin and Stat1. These findings reveal an epigenetic mechanism responsible for maintaining bone homeostasis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Henry C. Hrdlicka, Renata C. Pereira, Bongjin Shin, Siu-Pok Yee, Alix C. Deymier, Sun-Kyeong Lee, Anne M. Delany
Summary: miR-29-3p plays a crucial role in regulating gene expression in osteoblasts and osteoclasts. Inhibition of miR-29-3p can affect bone formation, while miR-29-3p inhibition combined with intermittent PTH treatment can enhance bone anabolism.
Article
Endocrinology & Metabolism
Yang Zhang, Xuping Wang, Xiaowen Huang, Lifeng Shen, Li Zhang, Dan Shou, Xiaohui Fan
Summary: The study identified miRNA-331-3p as a novel biomarker for regulating infected bone nonunion, which affects osteoblast mineralization by targeting FGF23. This finding provides a new perspective for the diagnosis and treatment of infected bone nonunion and other inflammatory bone disorders.
Review
Biochemistry & Molecular Biology
Mays Abuhantash, Emma M. Collins, Alexander Thompson
Summary: The HOXA gene cluster plays a crucial role in maintaining blood cell numbers and differentiation state, particularly in the emergence and long-term repopulation of hematopoietic stem cells. Dysregulation of HOXA genes is associated with malignant phenotypes, highlighting the need for further research to understand their specific roles in normal and malignant hematopoiesis.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Review
Biochemistry & Molecular Biology
Nicholas Smith, Suzanna Shirazi, Dimitrios Cakouros, Stan Gronthos
Summary: Many important epigenetic changes occur during early skeletal development and throughout life, influenced by factors such as aging, disease, and lifestyle. Epigenetics explores the heritable changes in gene expression resulting from environmental changes without any alterations in DNA sequence. Cellular epigenetic profiles are dynamic and regulated through mechanisms including histone modifications, non-coding RNA-associated gene silencing, and DNA methylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Alann Thaffarell Portilho Souza, Helena Bacha Lopes, Gileade Pereira Freitas, Emanuela Prado Ferraz, Fabiola Singaretti Oliveira, Adriana Luisa Goncalves Almeida, Denise Weffort, Marcio Mateus Beloti, Adalberto Luiz Rosa
JOURNAL OF BONE AND MINERAL METABOLISM
(2020)
Article
Dentistry, Oral Surgery & Medicine
N. P. Bueno, M. L. Bergamini, F. M. Elias, P. H. Braz-Silva, E. P. Ferraz
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2020)
Article
Biochemistry & Molecular Biology
Fernando Amorim Mendonca Alves, Marcia Martins Marques, Samantha Christine Santos Xisto Braga Cavalcanti, Ana Clara Fagundes Pedroni, Emanuela Prado Ferraz, Thais Gimienez Miniello, Maria Stella Moreira, Tomais Jeronimo, Maria Cristina Zindel Deboni, Cesar Angelo Lascala
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2020)
Article
Dentistry, Oral Surgery & Medicine
Lucas Moreira Mendonca, Hugo Gaeta-Araujo, Pedro Bastos Cruvinel, Ingrid Wenzel Tosin, Marcelo Rodrigues Azenha, Emanuela Prado Ferraz, Christiano Oliveira-Santos, Camila Tirapelli
Summary: This study observed the impact of changes in diagnosis caused by analysis of three-dimensional images on the treatment plans of impacted lower third molars. The results showed differences between panoramic and cone beam computed tomography assessments, with changes in CBCT examinations affecting intraoperative planning and expectation of post-operative complications. The study concluded that alterations in treatment plans can be influenced by changes in diagnosis after CBCT examination.
DENTOMAXILLOFACIAL RADIOLOGY
(2021)
Article
Biochemical Research Methods
Natalia Pieretti Bueno, Isabella Nunes Copete, Helena Bacha Lopes, Praveen R. Arany, Marcia Martins Marques, Emanuela Prado Ferraz
Summary: The study evaluated the effect of photobiomodulation therapy on osteoblast differentiation in diabetic rats, showing that PBM therapy improved the viability of induced diabetic rats' osteoblasts, increased the expression of bone markers, alkaline phosphatase activity, and mineralized matrix production. PBM therapy represents an innovative therapeutic approach to optimize the treatment of bone defects in diabetic patients.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Materials Science, Biomaterials
Roger Borges, Leticie M. Ferreira, Carlos Rettori, Isabella M. Lourenco, Amedea B. Seabra, Frank A. Mueller, Emanuela Prado Ferraz, Marcia M. Marques, Marta Miola, Francesco Baino, Javier B. Mamani, Lionel F. Gamarra, Juliana Marchi
Summary: This article introduces a new nanocomposite made of superparamagnetic iron oxide nanoparticles (SPIONs) dispersed in a sol-gel-derived bioactive glass matrix, which does not require any thermal treatment for crystallization of magnetic phases. The nanocomposite exhibits proper behavior for hyperthermia applications and shows apatite-forming properties similar to the highly bioactive parent glass, as well as osteoinductive properties.
BIOMATERIALS ADVANCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Lilian Victoria Perez Espinola, Ricardo Pimenta D'avila, Constantin Alexander Landes, Emanuela Prado Ferraz, Joao Gualberto Cerqueira Luz
Summary: This study investigated the impact of different stages of orthodontic-surgical treatment on the quality of life and self-esteem of patients with dentofacial deformity. The results showed that the dental decompensation stage did not negatively affect patient's confidence and well-being, but the main impact on patient's quality of life and self-esteem was evidenced after the removal of the orthodontic appliance.
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2022)
Article
Engineering, Biomedical
Natacha Kalline de Oliveira, Emanuela Prado Ferraz, Flavia Cristina Perillo Rosin, Luciana Correa, Maria Cristina Zindel Deboni
Summary: This study evaluates the angiogenesis response of a poly epsilon-caprolactone/poly (rotaxane) blend, and shows that it does not impair angiogenesis. Furthermore, the blend carried by dental pulp stem cells exhibits better vasculogenic potential.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Rayana L. L. Bighetti-Trevisan, Natalia P. Bueno, Alann T. P. Souza, Marcia M. Marques, Adalberto L. L. Rosa, Marcio M. M. Beloti, Emanuela P. P. Ferraz
Summary: Adipose tissue is a potential source of mesenchymal stem cells (MSCs), but their limited osteogenic potential hinders their use in bone regeneration. The release of TNF-alpha by adipose tissue can have a negative effect on the differentiation of MSCs into osteoblasts. By targeting TNF-alpha receptors, the inhibition of TNF-alpha can enhance bone formation in MSCs.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Lilian Victoria Perez Espinola, Ricardo Pimenta D'avila, Natalia Pieretti Bueno, Emanuela Prado Ferraz, Joao Gualberto Cerqueira Luz
Summary: This study aimed to evaluate the effectiveness of 940-nm laser photobiomodulation (PBM) therapy in patients undergoing orthognathic surgery. Twenty patients were randomly assigned to either a laser group or a control group. PBM was administered immediately after surgery and continued at regular intervals for four weeks. Parameters such as pain, edema, trismus, and paresthesia were evaluated. The results showed that PBM therapy effectively reduced postoperative pain and improved trismus.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Engineering, Biomedical
Leticia Faustino Adolpho, Larissa Mayra Silva Ribeiro, Gileade Pereira Freitas, Helena Bacha Lopes, Maria Paula Oliveira Gomes, Emanuela Prado Ferraz, Rossano Gimenes, Marcio Mateus Beloti, Adalberto Luiz Rosa
Summary: This study produced and characterized a P(VDF-TrFE)/BaTiO3 scaffold and evaluated its combination with MSCs and PBM for bone repair. The results showed that the scaffold acted synergistically with MSCs and PBM to induce bone repair in rat calvarial defects.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Camila Maciel Benassi, Vinicius Paes de Assis Santos, Guilherme Spagnol, Emanuela Prado Ferraz, Joao Gualberto C. Luz
Summary: This study evaluated records of patients with maxillofacial trauma due to interpersonal violence (IPV) being treated in the emergency room of a level I trauma center hospital in Sao Paulo, Brazil. Data of patients with maxillofacial trauma due to IPV recorded between January 2019 and December 2019 were retrospectively examined.
DENTAL TRAUMATOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Rayana Longo Bighetti-Trevisan, Alann Thaffarell Portilho Souza, Ingrid Wezel Tosin, Natalia Pieretti Bueno, Murilo Camuri Crovace, Marcio Mateus Beloti, Adalberto Luiz Rosa, Emanuela Prado Ferraz
Summary: The study investigated the effects of BioS-2P on osteoclast differentiation and activity in vitro and in vivo. It was found that BioS-2P promoted osteoclast viability and activity, and facilitated interactions between bone tissues and biomaterials.
BRAZILIAN ORAL RESEARCH
(2022)
Meeting Abstract
Endocrinology & Metabolism
Rayana Longo Bighetti-Trevisan, Natalia Bueno, Giovana Sanchez, Gileade Freitas, Marcio Beloti, Emanuela Ferraz
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Engineering, Biomedical
Natalia Pieretti Bueno, Cecilia Cardoso Kfouri, Isabella Nunes Copete, Fabiola Singaretti de Oliveira, Praveen Arany, Marcia Martins Marques, Emanuela Prado Ferraz
Summary: Diabetes negatively affects the osteogenic potential of bone marrow mesenchymal stem cells, leading to increased adipocyte differentiation. Photobiomodulation therapy can improve this condition by promoting osteoblast differentiation and potentially optimizing bone regeneration in diabetic patients.
LASERS IN MEDICAL SCIENCE
(2022)