Article
Multidisciplinary Sciences
Hiroki Takahashi, Ge Yang, Takeshi Yoneshiro, Yohei Abe, Ryo Ito, Chaoran Yang, Junna Nakazono, Mayumi Okamoto-Katsuyama, Aoi Uchida, Makoto Arai, Hitomi Jin, Hyunmi Choi, Myagmar Tumenjargal, Shiyu Xie, Ji Zhang, Hina Sagae, Yanan Zhao, Rei Yamaguchi, Yu Nomura, Yuichi Shimizu, Kaito Yamada, Satoshi Yasuda, Hiroshi Kimura, Toshiya Tanaka, Youichiro Wada, Tatsuhiko Kodama, Hiroyuki Aburatani, Min-Sheng Zhu, Takeshi Inagaki, Timothy F. Osborne, Takeshi Kawamura, Yasushi Ishihama, Yoshihiro Matsumura, Juro Sakai
Summary: Protein kinase A promotes beige adipogenesis by phosphorylating proteins, including histone H3 lysine 9 demethylase JMJD1A. This process needs to be reversible and is regulated by MYPT1-PP1β phosphatase activity. This study uncovers regulatory cross-talk involved in beige adipogenesis that coordinates epigenetic regulation with direct activation of YAP/TAZ transcriptional co-activators.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
B. Baran, K. Kosieradzka, W. Skarzynska, P. Niewiadomski
Summary: In this study, three novel kinases, MRCK alpha, MRCK beta, and MAP4K5, were identified to interact with Gli proteins and phosphorylate Gli2 on multiple sites. It was demonstrated that MRCK alpha/beta kinases regulate Gli proteins, which in turn affects the transcriptional output of the Hedgehog pathway. The research provides important insights into the phosphorylation-based activation mechanisms of Gli proteins.
CELLULAR SIGNALLING
(2023)
Article
Plant Sciences
Deepanjali Verma, Prakash Kumar Bhagat, Alok Krishna Sinha
Summary: This study demonstrates that the dual-specificity phosphatase MKP1 negatively regulates the activity of MPK6, a key negative regulator of blue light (BL)-mediated photomorphogenic development, by dephosphorylating it. Overexpression of MKP1 enhances BL-induced inhibition of hypocotyl elongation and affects downstream light-related gene expression in response to BL. This highlights the critical role of phosphatases in regulating signaling pathways and identifies the specific role of MKP1 in modulating BL-induced photomorphogenic responses.
Article
Microbiology
Haleema S. Malik, Flora Magnotti, Nicole A. Loeven, Jose M. Delgado, Arminja N. Kettenbach, Thomas Henry, James B. Bliska
Summary: This study found that PPP positively regulates the oligomerization, dephosphorylation, and second-site phosphorylation of Pyrin in mice and humans, playing an important role in the positive regulation of Pyrin after RhoA inactivation.
Article
Multidisciplinary Sciences
Tenghan Zhuang, Boyan Zhang, Yihong Song, Fan Huang, Wangfei Chi, Guangwei Xin, Ziyu Zhang, Steven Y. Cheng, Qing Jiang, Chuanmao Zhang
Summary: Sufu, as a negative regulator, plays a critical role in both centrosome and nuclear localization, regulating centrosome duplication and DNA replication by facilitating CP110 phosphorylation and Cdt1 degradation. Serum stimulation leads to the removal of Sufu from the centrosome and nucleus, promoting cell cycle progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Zarka Sarwar, Nusrat Nabi, Sameer Ahmed Bhat, Syed Qaaifah Gillani, Irfana Reshi, Misbah Un Nisa, Guillaume Adelmant, Jarrod A. Marto, Shaida Andrabi
Summary: In this study, we found that DBC1 interacts with PyST in mammalian cells, leading to the downregulation of DBC1 protein levels. Additionally, LKB1 can degrade DBC1 in an AMP kinase-independent manner and negatively regulates AKT1 activity through DBC1 and its downstream substrate TRB3. Furthermore, we demonstrated that DBC1 has oncogenic potential. Our findings provide insights into the LKB1-DBC1-AKT1 signaling paradigm and its role in cell cycle regulation, apoptosis, and tumorigenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Virology
Hong-Shuai Gao, Rong-Min Hu, Ze-Hua Wang, Xi-Qian Ye, Xiao-Tong Wu, Jian-Hua Huang, Zhi-Zhi Wang, Xue-Xin Chen
Summary: In this study, a PTP gene, CvBV_12-6, derived from CvBV was characterized and found to negatively regulate the immune response in the host P. xylostella. It suppressed the phenoloxidase activity and antibacterial abilities of hemocytes, as well as reduced the host's melanization and viability.
Article
Cell & Tissue Engineering
Chanyi Lu, Xiaoli Lin, Jumpei Yamashita, Ranhui Xi, Minliang Zhou, Yali Zhang, Hong Wang, Robert E. Margolskee, Bon-Kyoung Koo, Hans Clevers, Ichiro Matsumoto, Peihua Jiang
Summary: The study reveals the important roles of RNF43/ZNRF3 in regulating taste and lingual epithelial tissue homeostasis. R-spondin interacts with RNF43/ZNRF3 to enhance WNT signaling, thereby controlling taste cell regeneration and lingual epithelial tissue homeostasis.
Article
Multidisciplinary Sciences
Xinzhi Li, Bingchuan Yuan, Min Lu, Yuqin Wang, Na Ding, Chunhong Liu, Ming Gao, Zhicheng Yao, Shiyan Zhang, Yujun Zhao, Liwei Xie, Zheng Chen
Summary: The study reveals that METTL3 negatively regulates NASH progression by inhibiting transcription of Cd36 and Ccl2 genes, playing a key role in the transition from NAFL to NASH.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Margot C. DeBaker, Eric H. Mitten, Timothy R. Rose, Ezequiel Marron Fernandez de Velasco, Runbo Gao, Anna M. Lee, Kevin Wickman
Summary: This study found that RGS6 protein negatively regulates inhibitory G protein signaling pathways in VTA dopamine neurons in mice and has a sex-dependent positive influence on binge-like alcohol consumption in adult mice.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Foltman, Ivan Mendez, Joan J. Bech-Serra, Carolina de la Torre, Jennifer L. Brace, Eric L. Weiss, Maria Lucas, Ethel Queralt, Alberto Sanchez-Diaz
Summary: This article investigates the negative regulatory mechanism of cell separation by TORC1 in budding yeast, revealing that TORC1 controls the process of separation by phosphorylating Cbk1 and also affects the activation of Sec3 on the SNARE complex. This discovery sheds light on the crucial role of TORC1 in cell separation.
Article
Engineering, Chemical
Fang Wang, Zhuang Liu, Lin-Bing Zou, Rui Xie, Xiao-Jie Ju, Wei Wang, Da-Wei Pan, Liang-Yin Chu
Summary: A universal model is developed to describe the responsive performances of both positively and negatively responsive smart gating membranes. The reliability and fitting precision of the proposed model have been validated by experimental results. The model provides new insights for describing and explaining the responsive gating performances and offers rational guidelines for designing and fabricating smart gating membranes with desired performances.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Oncology
Dongmei Bai, Yong Wu, Poonamjot Deol, Yumiko Nobumori, Qi Zhou, Frances M. Sladek, Xuan Liu
Summary: Chronic elevation of free fatty acid levels is associated with metabolic disorders and tumorigenesis, and high fat diet-induced increase in palmitic acid may lead to PTEN down-regulation through mTOR/S6K-mediated phosphorylation. This phosphorylation enhances PTEN interaction with WWP2, promoting PTEN polyubiquitination, degradation, and subsequent AKT activation and cell proliferation. These findings highlight a molecular mechanism by which high fat diet and elevated palmitic acid regulate cell proliferation through inactivation of the tumor suppressor PTEN.
Article
Oncology
Yiming Sun, Qiong Pan, Linyan Ma, Chao Chen, Surong Zhao, Hao Liu
Summary: Up to 30% of breast cancer mortality is caused by cancer relapse due to distant metastases despite primary clinical treatments. Further research on breast cancer mechanisms is needed for a deeper understanding of disease prognosis. The glycolysis inhibitor 3-bromopyruvate (3-BP) has shown potential as an antitumor agent, inhibiting cell proliferation in MDA-MB-231 and MCF-7 breast cancer cells through energy metabolism inhibition. Additionally, 3-BP treatment in an MDA-MB-231 cell-transplanted mouse model demonstrated a significant antitumor effect correlated with necroptosis-related protein Ppm1b.
FRONTIERS IN ONCOLOGY
(2021)
Article
Plant Sciences
KwiMi Chung, Agnes J. Demianski, Gregory A. Harrison, Neva Laurie-Berry, Nobutaka Mitsuda, Barbara N. Kunkel
Summary: JAH3 is a negative regulator of both JA and ET signaling pathways, as demonstrated by hypersensitivity phenotypes in jah3 mutants and their interactions with COI1 and EIN3 components.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Clinical Neurology
Kazuhiko Watabe, Yoichiro Kato, Miho Sakuma, Makiko Murata, Motoko Niida-Kawaguchi, Taro Takemura, Nobutaka Hanagata, Mari Tada, Akiyoshi Kakita, Noriyuki Shibata
Article
Biochemistry & Molecular Biology
Anh Thi Tram Tu, Kazuaki Hoshi, Kazunori Ikebukuro, Nobutaka Hanagata, Tomohiko Yamazaki
Article
Chemistry, Physical
Chiho Kataoka-Hamai, Kohsaku Kawakami
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: The study investigated the adsorption of phase-separated GUVs on glass, revealing that both liquid-ordered and liquid-disordered domains are reorganized to stabilize the curved bilayer region in adsorbed GUVs.
Article
Chemistry, Multidisciplinary
Hongxin Wang, Han Zhang, Bo Da, Dabao Lu, Ryo Tamura, Kenta Goto, Ikumu Watanabe, Daisuke Fujita, Nobutaka Hanagata, Junko Kano, Tomoki Nakagawa, Masayuki Noguchi
Summary: Cellular mechanical properties can serve as potential cancer biomarkers for objective cytology, with a classifier trained through high dimensional mechanome data achieving a classification accuracy of 89% and instrument-independent robustness. This approach also demonstrated a signal-to-noise ratio 8 times higher than traditional morphology-based methods in differentiating precancerous cells from normal cells.
Article
Multidisciplinary Sciences
Nobutaka Hanagata, Taro Takemura, Keiko Kamimura, Toshiaki Koda
SCIENTIFIC REPORTS
(2020)
Article
Pharmacology & Pharmacy
Tsu-Man Chiu, Ping-Chun Hsu, Mohd Yaqub Khan, Cheng-An J. Lin, Chun-Hung Lee, Tsai-Ching Hsu, Min-Hua Chen, Nobutaka Hanagata
Summary: Warts are a common skin problem caused by viral infection. Current treatments for warts, such as ablating tissue or interrupting cellular division, can be invasive or cause skin discomfort. Imiquimod is a synthetic compound used topically to treat anogenital warts, but it has limitations such as low skin permeability and side effects. Microneedle technology shows promise in overcoming these barriers and providing painless and personalized treatment options.
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: Phospholipid monolayers at oil-water interfaces are used for exploring biological interface properties, with researchers developing methods to quantify mono-layer system characteristics. By calculating surface pressure Pi and multiplying it by estimated values, the dependence of Pi on membrane area a was determined through investigations on POPC monolayers at different interfaces.
Article
Chemistry, Multidisciplinary
Irem Nur Gamze Ozbilgin, Tomohiko Yamazaki, Junpei Watanabe, Hong-Tao Sun, Nobutaka Hanagata, Naoto Shirahata
Summary: We report carboxy-terminated silicon quantum dots (SiQDs) that are highly soluble in water due to the high coverage of surface monolayers, exhibit bright light emission with high photoluminescence quantum yields (PLQYs), show long-term stability in PL property for cell monitoring, have low toxicity to cells, and display a high photothermal response.
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: Phospholipid monolayers formed at oil-water interfaces have been studied for various biological applications, with the interaction between the monolayers and hydrocarbons depending on the molecular structure, hydrophobic chain length, and area compression.
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: In this study, the adsorption of triacylglycerol droplets onto glass-supported phospholipid bilayers was investigated using fluorescence microscopy. The results showed that triacylglycerol droplets were immobilized in the bilayer membrane and their volume varied over time. Phospholipids on and near the droplets were fully mobile, and triacylglycerol molecules diffused between different droplets along the bilayer. The size distribution of the droplets also changed over time.
Article
Green & Sustainable Science & Technology
Kohsaku Kawakami, Junko Tanaka, Chiho Kataoka-Hamai
Summary: Phospholipid, as a natural and abundant resource, can be used as functional building blocks for various soft materials. In this study, mesoporous phospholipid particles (MPPs) were utilized to stabilize liquid/liquid interfaces and produce oil-in-water emulsions. The MPPs, composed of lipid bilayers and approximately 10 μm in size, showed high surface activity and could adsorb at the oil/water interface to form stable emulsions. The destabilization mechanism of the emulsion depended on the presence of charged molecules on the MPPs, with aggregation occurring in the case of charged MPPs and coalescence in the case of non-charged MPPs. These materials have potential applications as more nature-friendly alternatives to surfactants for oil adsorption/recovery in seawater and soils.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Miyuki Tabata, Chiho Kataoka-Hamai, Kozue Nogami, Daiju Tsuya, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
Summary: A solid-state potentiometric biosensor based on mixed phase modification of a silver surface was proposed in this study, achieving stable electromotive force and biomolecule functionalization simultaneously. The modified surface reduced nonspecific binding of interfering biomolecules and maintained stability under constant chloride ion concentrations. With a low detection limit of 0.1 pM for microRNA 146, this biosensor could be useful as a disposable single-use sensor in medical fields.
Article
Chemistry, Multidisciplinary
Shanmugavel Chinnathambi, Nobutaka Hanagata, Tomohiko Yamazaki, Naoto Shirahata
Article
Engineering, Electrical & Electronic
Tao Liang, Mohammad Rezwan Habib, Han Xiao, Shuang Xie, Yuhan Kong, Cui Yu, Hideo Iwai, Daisuke Fujita, Nobutaka Hanagata, Hongzheng Chen, Zhihong Feng, Mingsheng Xu
ACS APPLIED ELECTRONIC MATERIALS
(2020)