Article
Biochemistry & Molecular Biology
Miguel A. Osorio Garcia, Elizabeth A. Wood, James L. Keck, Michael M. Cox
Summary: The bacterial RadD enzyme plays an important role in multiple genome maintenance pathways, but its specific functions are not well understood. One potential clue is its interaction with the single-stranded DNA binding protein (SSB), which stimulates RadD activity. By studying RadD-SSB complex formation, researchers found that a specific pocket on RadD is essential for SSB binding. Mutations in this pocket hindered complex formation and eliminated SSB's stimulation of RadD activity. Moreover, mutant strains of E. coli with altered RadD-SSB interaction exhibited increased sensitivity to DNA damaging agents. This suggests that an intact interaction with SSB is necessary for RadD's full function.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Piero R. Bianco, Yue Lu
Summary: In Escherichia coli, DNA replication forks stall at least once per cell cycle, and the process of rescuing the stalled forks involves the sequential action of different proteins or enzymes depending on the nature of the impediment. Insight gained from single-molecule studies provides a comprehensive understanding of the roles of individual proteins and pathways in stalled DNA replication fork rescue.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xinxing Lyu, Kai-Hang Lei, Pau Biak Sang, Olga Shiva, Megan Chastain, Peter Chi, Weihang Chai
Summary: The CST complex protects stalled replication forks from degradation by the MRE11 nuclease by inhibiting MRE11 binding to reversed forks, thus preventing excessive nascent-strand degradation and preserving genome integrity under replication stress, particularly in BRCA2 deficient cells.
Article
Medicine, Research & Experimental
Bassam Abu-Libdeh, Satpal S. Jhujh, Srijita Dhar, Joshua A. Sommers, Arindam Datta, Gabriel M. C. Longo, Laura J. Grange, John J. Reynolds, Sophie L. Cooke, Gavin S. McNee, Robert Hollingworth, Beth L. Woodward, Anil N. Ganesh, Stephen J. Smerdon, Claudia M. Nicolae, Karina Durlacher-Betzer, Vered Molho-Pessach, Abdulsalam Abu-Libdeh, Vardiella Meiner, George-Lucian Moldovan, Vassilis Roukos, Tamar Harel, Robert M. Brosh, Grant S. Stewart
Summary: This study identified a genetic instability disorder named RECON syndrome, caused by mutations in the RECQL gene. The affected individuals exhibited multiple symptoms, such as short stature and progeroid facial features. This research expands the association of the RecQ helicase family with human genomic stability disorders.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Swagata Halder, Aurore Sanchez, Lepakshi Ranjha, Giordano Reginato, Ilaria Ceppi, Ananya Acharya, Roopesh Anand, Petr Cejka
Summary: RAD51 and BRCA2 play critical roles in DNA repair and protection. RAD51 binds to single-stranded DNA for its recombination function, but binding to double-stranded DNA is inhibitory. Surprisingly, we found that the protective function of RAD51 actually depends on its binding to double-stranded DNA. The BRC4 repeat of BRCA2 disrupts RAD51 binding to double-stranded DNA and impairs its protective function. However, the C-terminal RAD51-binding segment (TR2) of BRCA2 overcomes the inhibitory effect of BRC4 and stabilizes RAD51 binding to double-stranded DNA, promoting DNA protection.
Article
Biochemistry & Molecular Biology
Luis Reza Herrera, Ronnesha A. Johnson, Kathleen McGlynn, Zane A. Gibbs, Anthony J. Davis, Angelique W. Whitehurst
Summary: Tumor-induced expression of meiotic genes, particularly HORMAD1, is associated with reduced patient survival and increased genomic instability. HORMAD1 plays a critical role in protecting stalled DNA replication forks and preventing DNA strand degradation. Loss of HORMAD1 leads to increased DNA breaks and chromosomal defects, exacerbated by induction of replication stress.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Cell Biology
Riko Ishimoto, Yota Tsuzuki, Tomoki Matsumura, Seiichiro Kurashige, Kouki Enokitani, Koki Narimatsu, Mitsunori Higa, Nozomi Sugimoto, Kazumasa Yoshida, Masatoshi Fujita
Summary: The study reveals that the accumulation of ssDNA and DDR proteins is induced by site-specific replication fork barriers on the human chromosome. SLX4-XPF acts as an upstream factor for the accumulation of DDR proteins, leading to the recruitment of ATR and FANCD2. Lac! binding in S phase results in underreplication and abnormal mitotic segregation of the lac) arrays. The SLX4-ATR axis represses anaphase abnormality induced by Lac! binding.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Brent De Wijngaert, Shemaila Sultana, Anupam Singh, Chhaya Dharia, Hans Vanbuel, Jiayu Shen, Daniel Vasilchuk, Sergio E. Martinez, Eaazhisai Kandiah, Smita S. Patel, Kalyan Das
Summary: The study presents structures of two intermediate states of yeast mtRNAP during transcription initiation, elucidating molecular mechanisms of different steps and explaining key processes of transcription initiation.
Article
Chemistry, Multidisciplinary
Hui Yin Tan, Piero R. Bianco
Summary: PriA plays a role in reloading the primosome onto resurrected replication forks, with SSB enhancing its ability to discriminate between fork substrates. The interaction between PriA and SSB increases the catalytic efficiency of the helicase, but also decreases its affinity for other forks. The unique ability of PriA to bind with high affinity to the 3'-OH at the end of the nascent leading strand, in the presence of SSB, ensures the correct loading onto the fork for directed replication restart.
Article
Toxicology
Hattaya Benya-aphikul, Varisa Pongrakhananon, Paninee Chetprayoon, Thongchai Sooksawate, Ratchanee Rodsiri
Summary: Prenatal exposure to methamphetamine disrupts synaptogenesis and impairs learning and memory, with alterations in the BDNF-TrkB signaling pathway. This disruption leads to long-lasting memory impairment in adolescent and adult mice.
TOXICOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Nicola Zilio, Helle D. Ulrich
Summary: Mapping the genome-wide distribution of single-strand breaks is crucial for understanding damage signaling and DNA repair. This article reviews classical and newly developed high-resolution methods for mapping single-strand breaks, highlighting the valuable insights they provide into the impact of this type of damage on the genome.
Article
Biochemistry & Molecular Biology
Seungwoo Chang, Elizabeth S. Thrall, Luisa Laureti, Sadie C. Piatt, Vincent Pages, Joseph J. Loparo
Summary: This study reveals the selective enrichment of bacterial TLS polymerase Pol IV at stalled replication forks by the fork-associated ssDNA-binding protein (SSB), enabling its association with the processivity clamp and facilitating TLS. The mechanism involves stalling-dependent changes in clusters of fork-associated SSB, establishing hierarchical access to the processivity clamp and prioritizing specific CLIPs with SSB-binding activity.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Environmental Studies
Bohuang Pan, Tomiwa Sunday Adebayo, Ridwan Lanre Ibrahim, Mamdouh Abdulaziz Saleh Al-Faryan
Summary: This research investigates the impact of nuclear energy on CO2 emissions in leading nuclear-consuming countries. The findings reveal that nuclear energy contributes to decreased environmental degradation in most cases and that nuclear energy consumption and CO2 emissions can predict each other to a large extent.
ENERGY & ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Olga V. Volodina, Arina A. Anuchina, Milyausha I. Zainitdinova, Nadezhda A. Evtushenko, Alexander V. Lavrov, Svetlana A. Smirnikhina
Summary: Gene editing allows targeted changes in the genome, potentially treating hereditary diseases. However, the effectiveness of gene editing methods in correcting mutations is still low. This study proposes the design of single-stranded oligodeoxyribonucleotides to improve the efficiency of gene editing, providing insights for developing gene therapy for hereditary diseases.
BIOCHEMISTRY-MOSCOW
(2022)
Article
Chemistry, Analytical
Szabolcs Sofalvi, Harold E. Schueler
Summary: Accredited forensic toxicology laboratories need to validate bioanalytical methods according to ISO/IEC 17025 standards to ensure fitness for intended use. A Microsoft Excel template was developed to rapidly generate validation results and provide traceability to instrument raw data, with key features including weighted linear calibration models and ANOVA tables for easy evaluation of validation data. This template saves time and streamlines the process for methods with multiple analytes, providing a two-page validation summary report along with additional digital information for electronic records.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Joseph T. P. Yeeles, Tom D. Deegan, Agnieszka Janska, Anne Early, John F. X. Diffley
Article
Biochemistry & Molecular Biology
Tom D. Deegan, Joseph T. P. Yeeles, John F. X. Diffley
Article
Biochemistry & Molecular Biology
Christoph F. Kurat, Joseph T. P. Yeeles, Harshil Patel, Anne Early, John F. X. Diffley
Article
Biochemistry & Molecular Biology
Joseph T. P. Yeeles, Agnieska Janska, Anne Early, John F. X. Diffley
Article
Biochemistry & Molecular Biology
Martin R. G. Taylor, Joseph T. P. Yeeles
Article
Biochemistry & Molecular Biology
Joseph T. P. Yeeles
BIOCHEMICAL SOCIETY TRANSACTIONS
(2014)
Article
Biochemistry & Molecular Biology
Carolina B. Gabbai, Joseph T. P. Yeeles, Kenneth J. Marians
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Sankalp Gupta, Joseph T. P. Yeeles, Kenneth J. Marians
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Joseph T. P. Yeeles, Kenneth J. Marians
Article
Multidisciplinary Sciences
Milind K. Gupta, Colin P. Guy, Joseph T. P. Yeeles, John Atkinson, Hazel Bell, Robert G. Lloyd, Kenneth J. Marians, Peter McGlynn
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Biochemistry & Molecular Biology
Valentina Aria, Joseph T. P. Yeeles
Article
Biochemistry & Molecular Biology
Martin R. G. Taylor, Joseph T. P. Yeeles
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Tom D. Deegan, Jonathan Baxter, Maria Angeles Ortiz Bazan, Joseph T. P. Yeeles, Karim P. M. Labib
Article
Biochemistry & Molecular Biology
Thomas A. Guilliam, Joseph T. P. Yeeles
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)