Article
Chemistry, Inorganic & Nuclear
Kady Oakley, Kevin Sterling, Jason Shearer, Eunsuk Kim
Summary: The study demonstrates how MitoNEET releases its [2Fe-2S] cluster under acidic conditions by controlling proton reactions to rearrange the cluster structure. Site-differentiated [2Fe-2S] clusters prepared experimentally undergo redox reactions with temperature changes.
INORGANIC CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Wei Lv, Julie A. Champion
Summary: The study focused on intracellular antibody delivery using the Hex antibody nanocarrier, showing that Hex-antibodies were internalized through multiple endocytic routes into lysosomes and released to the cytosol, retaining their bioactivity for at least 24 hours. The functional effect of Hex delivered anti-STAT3 antibodies was evidenced by inhibition of nuclear translocation of the cytosolic transcription factor STAT3.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Parool Gupta, Rani Mansuri, Priya Priydarshni, Sachidananda Behera, Amir Zaidi, Shamshun Nehar, Ganesh Chandra Sahoo, Krishna Pandey, Vahab Ali
Summary: This study revealed the interaction between two proteins, Cfd1 and Nbp35, in L. donovani, forming a stable complex involved in the cellular process of Fe-S cluster biogenesis and transfer. The upregulation of Cfd1 and Nbp35 proteins in drug-resistant parasites leads to increased Fe-S protein levels, promoting parasite survival under drug pressure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Genetics & Heredity
Yang Lyu, Zhongqi Liufu, Juan Xiao, Tian Tang
Summary: The evolution of a microRNA cluster, mir-972C, in Drosophila rewires its downstream regulatory networks. Both young and old mir-972C members evolved rapidly in seed and non-seed regions, leading to extensive target divergence among different Drosophila species.
FRONTIERS IN GENETICS
(2021)
Article
Genetics & Heredity
Nidhi Kirtikumar Bub, Sakshi Anand, Swati Garg, Vishal Saxena, Dhanabala Subhiksha Rajesh Khanna, Deeptanshu Agarwal, Sanjay Kumar Kochar, Shailja Singh, Shilpi Garg
Summary: This study identifies Dre2 as a potential target for artemisinin drugs and demonstrates that artemisinin derivatives can interact with Dre2, altering its activity and inducing cell death.
Article
Genetics & Heredity
Yang Zhang, Fei He, Yanning Zhang, Qian Dai, Qintong Li, Jing Nan, Ruidong Miao, Bo Cheng
Summary: This study discovered intrinsic sequence correlations among intra-cluster KRAB-Zfp members, and identified the crucial role of conserved cluster-specific linkers in the evolution and target specificity of KRAB-Zfps. An effective CRISPR-Cas9 based approach was developed to rapidly edit KRAB-Zfp clusters and investigate the regulatory correlation between cluster members and potential TE targets.
Article
Chemistry, Medicinal
Florian Malard, Eric Jacquet, Naima Nhiri, Christina Sizun, Amelie Chabrier, Samir Messaoudi, Jerome Dejeu, Stephane Betzi, Xu Zhang, Aurelien Thureau, Ewen Lescop
Summary: TCTP protein is a pharmacological target in cancer, and its interactions with drugs such as sertraline and thioridazine have been explored using multiple techniques. The study revealed weaker drug binding compared to previous reports and found that phosphorylation at S46 does not enhance the interaction between TCTP and sertraline. Alternative modes of action for the drugs in tumor cells are discussed in light of these findings.
Article
Biochemistry & Molecular Biology
Christophe Velours, Jingjing Zhou, Paolo Zecchin, Nisha He, Myriam Salameh, Marie-Pierre Golinelli-Cohen, Beatrice Golinelli-Pimpaneau
Summary: Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS) is a technique used to determine the absolute molar mass of macromolecules in solution by detecting their light scattering intensity. This technique can be applied to proteins and polymers of any shape without relying on assumptions or calibrations. However, corrections are needed for samples that absorb light at the MALS laser wavelength. In this study, various corrections were applied to determine the absolute molar mass of [2Fe-2S] and [4Fe-4S] cluster-containing proteins in both apo- and holo-forms. The determination of the absolute molar mass of the [2Fe-2S]-containing holo-NEET proteins revealed changes in oligomerization state upon cluster binding, highlighting an essential function of the cluster.
Article
Virology
Assia Mouhand, Loussine Zargarian, Anissa Belfetmi, Marjorie Catala, Marco Pasi, Ewen Lescop, Carine Tisne, Olivier Mauffret
Summary: The nucleocapsid domain (NCd) of HIV-1 Gag protein plays a crucial role in various stages of the replication cycle and can exist in different forms throughout the viral life cycle. Conformational equilibria between a major state and two minor states in the ZK2 of NCd were highlighted, with the minor states being most prominent at physiological temperature.
Article
Multidisciplinary Sciences
Alexandre Pozza, Francois Giraud, Quentin Cece, Marina Casiraghi, Elodie Point, Marjorie Damian, Christel Le Bon, Karine Moncoq, Jean-Louis Baneres, Ewen Lescop, Laurent J. Catoire
Summary: This study provides a detailed description of the close relationships between membrane proteins and lipids using lipid nanodiscs and high-pressure NMR. The results show a link between pressure and lipid compositions to the conformational landscape of the membrane proteins. The study also reveals that pressure can modify the conformation and gelation of lipids, and that membrane proteins can modulate the fluidity of the bilayer.
NATURE COMMUNICATIONS
(2022)
Article
Computer Science, Software Engineering
Florian Malard, Laura Danner, Emilie Rouzies, Jesse G. Meyer, Ewen Lescop, Stephanie Olivier-Van Stichelen
Summary: This article introduces EpyNN, an educational Python resource designed to help the public understand key concepts and practical implementation of scalable artificial neural network (ANN) architectures. EpyNN includes an educational API and workflows, as well as a documentation website that provides learning materials with code, mathematics, graphical representation, and text side-by-side.
Article
Environmental Sciences
Severine A. Degrelle, Ioana Ferecatu, Thierry Fournier
Summary: Aryl hydrocarbon receptor (AHR) is a crucial transcription factor involved in various biological processes. Two new reporter genes have been constructed to improve the study of AHR activity, and their capability to monitor AHR activity in different cell types has been demonstrated.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Clinical Neurology
Arnaud Jacquier, Julian Theuriet, Fanny Fontaine, Valentine Mosbach, Nicolas Lacoste, Shams Ribault, Valerie Risson, Julien Carras, Laurent Coudert, Thomas Simonet, Philippe Latour, Tanya Stojkovic, Juliette Piard, Anne Cosson, Gaetan Lesca, Francoise Bouhour, Stephane Allouche, Helene Puccio, Antoine Pegat, Laurent Schaeffer
Summary: Jacquier et al. identified a homozygous variant in the COQ7 gene in a family with distal hereditary motor neuropathy. This variant leads to a decrease in coenzyme Q10 production, causing impaired mitochondrial metabolism. Coenzyme Q10 supplementation may serve as a potential treatment for this disorder.
Article
Genetics & Heredity
Louise A. Corben, Veronica Collins, Sarah Milne, Jennifer Farmer, Ann Musheno, David Lynch, Sub Subramony, Massimo Pandolfo, Joerg B. Schulz, Kim Lin, Martin B. Delatycki
Summary: To improve clinical care for FRDA patients, the GRADE rare-disease framework was adopted, incorporating additional strategies to support the strength of recommendations. The study generated 130 new recommendations and 95 best practice statements.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Clinical Neurology
Ioannis Manolaras, Andrea Del Bondio, Olivier Griso, Laurence Reutenauer, Aurelie Eisenmann, Bianca H. Habermann, Helene Puccio
Summary: Manolaras et al. demonstrate that COQ8A-ataxia is primarily caused by mitochondrial dysfunction leading to calcium dysregulation in Purkinje neurons of the cerebellum. These abnormalities can be rescued in vitro by treatment with coenzyme Q10. It is a rare form of neurodegenerative disorder due to mutations in the COQ8A gene. The loss of COQ8A in Purkinje neurons is the main cause of cerebellar ataxia, resulting in abnormal dendritic arborizations, altered mitochondria function, and intracellular calcium dysregulation. Furthermore, they show that oxidative phosphorylation, particularly Complex IV, is primarily altered at presymptomatic stages of the disease, and CoQ10 treatment could rescue the pathology.
Article
Biochemistry & Molecular Biology
Gaelle Deval, Margaux Nedder, Severine Degrelle, Jasmina Rogozarski, Marie-Leone Vignaud, Audrey Chissey, Stacy Colzin, Christelle Laguillier-Morizot, Xavier Coumoul, Sonja Boland, Thierry Fournier, Amal Zerrad-Saadi, Ioana Ferecatu
Summary: Studying the impact of pollutants on the human placenta is crucial as they can accumulate in placental cells or pass into the fetal circulation. This study aimed to identify the signaling pathways modulated after exposure to benzo(a)pyrene (BaP) and cerium dioxide nanoparticles (CeO2 NP) individually or in combination. BaP, when bioactivated by xenobiotic metabolizing enzymes, leads to DNA damage and stress response in the placental cells. CeO2 NP, on the other hand, has an antioxidant effect. Co-exposure to these two pollutants modulates the genotoxic effect of BaP and reduces oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Genetics & Heredity
Cindy Vallieres, Marie-Pierre Golinelli-Cohen, Olivier Guittet, Michel Lepoivre, Meng-Er Huang, Laurence Vernis
Summary: Redox homeostasis is a crucial process that maintains a balance between reducing and oxidizing reactions within cells. Imbalanced redox homeostasis can lead to various diseases, including cancer and inflammation. Disrupting redox balance has been used as a strategy for cancer treatment, while maintaining selectivity between cancer and normal cells is important to avoid toxicity. Redox-based approaches are also employed in tackling infectious diseases with limited impacts on host cells. This review focuses on recent advances in redox-based strategies to fight eukaryotic pathogens, particularly fungi and eukaryotic parasites.
Article
Biochemistry & Molecular Biology
Lea Poinsignon, Audrey Chissey, Ayoub Ajjaji, Isabelle Hernandez, Marie-Leone Vignaud, Ioana Ferecatu, Thierry Fournier, Jean-Louis Beaudeux, Amal Zerrad-Saadi
Summary: The placenta undergoes significant changes during fetal development, and the activity of NADPH oxidase and the expression of NOX family proteins in the placenta are associated with physiological and pathological pregnancies. In cases of preeclampsia, the expression and regulation of NOX family proteins are altered, suggesting their involvement in the development of the disease. Mapping the placental NOX family proteins is important for future research on the pathophysiology of preeclampsia.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Neurosciences
Danique Beijer, Brent Fogel, Sergi Beltran, Matt Danzi, Andrea Nemeth, Stephan Zuchner, Matthis Synofzik
Summary: The Ataxia Global Initiative (AGI) is a global research platform aiming to enhance trial-readiness in degenerative ataxias. The AGI NGS working group focuses on improving methods for ataxia NGS analysis and data sharing to increase the number of genetically diagnosed ataxia patients. Collaboration between AGI and other research platforms has led to the diagnosis of over 500 ataxia patients and the discovery of more than 30 novel ataxia genes. The AGI NGS working group presents consensus recommendations for NGS data sharing initiatives, emphasizing harmonized variant analysis and standardized clinical and metadata collection.
Article
Clinical Neurology
Chiara Dionisi, Marine Chazalon, Myriam Rai, Celine Keime, Virginie Imbault, David Communi, Helene Puccio, Serge N. Schiffmann, Massimo Pandolfo
Summary: This study characterizes the abnormalities in neurons of Friedreich ataxia, demonstrating impaired cytoskeleton organization, neurite extension, synaptic plasticity, and altered electrophysiological profiles. Despite restoration of FXN expression, isogenic control neurons still exhibit characteristics of Friedreich ataxia neurons.
BRAIN COMMUNICATIONS
(2022)
Article
Chemistry, Inorganic & Nuclear
Tyler L. Perfitt, Alain Martelli
Summary: Iron-sulfur clusters are essential cofactors in biological pathways, and their biosynthesis relies on mitochondria. The Fe-S core complex plays a crucial role in the de novo assembly process, consisting of various proteins. Recent advances have provided insights into the structure and function of the Fe-S core complex.
