期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 289, 期 18, 页码 12457-12466出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.521708
关键词
Cell Death; Cell Signaling; Inflammation; Small Molecules; Tumor Necrosis Factor (TNF); Computer Modeling; Surface Plasmon Resonance (SPR)
资金
- National Basic Research Program of China [2012CB966403]
- Chinese National Natural Science Foundation [31271484, 31171431, 30400405]
- Tianjin Natural Science Foundation [12JCZDJC24600, 11JCYBJC27300]
Background: Most commercial TNF inhibitors are biomacromolecules. Results: A lead compound named C87 was identified using computer-aided drug design and could attenuate murine acute hepatitis. Conclusion: C87 was one of the first effective small-molecule inhibitors of TNF identified to date. Significance: The study highlights the effectiveness of combining virtual screening with functional assays for developing novel small-molecule TNF inhibitors. Overexpression of tumor necrosis factor (TNF) is a hallmark of many inflammatory diseases, including rheumatoid arthritis, inflammatory bowel disease, and septic shock and hepatitis, making it a potential therapeutic target for clinical interventions. To explore chemical inhibitors against TNF activity, we applied computer-aided drug design combined with in vitro and cell-based assays and identified a lead chemical compound, (E)-4-(2-(4-chloro-3-nitrophenyl) (named as C87 thereafter), which directly binds to TNF, potently inhibits TNF-induced cytotoxicity (IC50 = 8.73 m) and effectively blocks TNF-triggered signaling activities. Furthermore, by using a murine acute hepatitis model, we showed that C87 attenuates TNF-induced inflammation, thereby markedly reducing injuries to the liver and improving animal survival. Thus, our results lead to a novel and highly specific small-molecule TNF inhibitor, which can be potentially used to treat TNF-mediated inflammatory diseases.
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