Article
Cell Biology
Haiying Liu, Linyu Dai, Ming Wang, Fumin Feng, Yonghong Xiao
Summary: This study demonstrated that tunicamycin can induce the apoptosis of HSCs by increasing intracellular Ca2+ concentration and enhancing the expression of ERS proteins, while decreasing the expression of antiapoptotic proteins. The findings suggest that tunicamycin induces HSC apoptosis through the calpain-2/Ca2+-dependent ERS pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Samuel C. Buchl, Zachary Hanquier, Andrew J. Haak, Yvonne M. Thomason, Robert C. Huebert, Vijay H. Shah, Jessica L. Maiers
Summary: The study identified TNIK as a key regulator of procollagen I trafficking in vitro and hepatic fibrogenesis in vivo, suggesting that inhibition of TNIK could be a potential therapeutic target for limiting liver fibrosis.
HEPATOLOGY COMMUNICATIONS
(2022)
Article
Gastroenterology & Hepatology
Xiao-Xue Hou, Yu-Wen Li, Jia-Li Song, Wen Zhang, Rui Liu, Hui Yuan, Tian-Tong Feng, Zheng-Yi Jiang, Wen-Ting Li, Chuan-Long Zhu
Summary: CPT promotes apoptosis of HSCs and alleviates hepatic fibrosis by modulating the ERS pathway.
WORLD JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Cell Biology
Vittorio Abbonante, Alessandro Malara, Martina Chrisam, Samuele Metti, Paolo Soprano, Claudio Semplicini, Luca Bello, Valeria Bozzi, Monica Battiston, Alessandro Pecci, Elena Pegoraro, Luigi De Marco, Paola Braghetta, Paolo Bonaldo, Alessandra Balduini
Summary: Endoplasmic reticulum stress is linked to collagen retention, apoptosis, and impaired autophagy in megakaryocytes. This study highlights the interplay between genetic disorders of collagens, endoplasmic reticulum stress, and autophagy regulation in megakaryocytes.
Article
Immunology
Erliang Kong, Yongchang Li, Xuqiang Geng, Jianxin Wang, Yan He, Xudong Feng
Summary: This study demonstrated that ischemic preconditioning (IPC) could protect against hepatic ischemia-reperfusion injury (HIRI) by activating autophagy and regulating endoplasmic reticulum stress (ERS)-dependent apoptosis in hepatocytes.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaohong Wang, Hairui Xin, Chuang Zhang, Xianhong Gu, Yue Hao
Summary: Endoplasmic reticulum stress (ERS) is closely related to the occurrence and progression of metabolic liver disease. In this study, ERS models were established on piglet livers and porcine hepatic stellate cells (HSCs) using tunicamycin. It was found that ERS significantly affected the secretion and synthesis of insulin-like growth factor and ubiquitin proteins. Knockdown of GRP94 in porcine HSCs resulted in decreased secretion of growth factors and increased apoptosis under ERS conditions. These findings provide important insights into the adaptive mechanisms of the liver under ERS and potential therapeutic targets for ERS-induced diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuan Hu, Chenyang Qi, Jiaoyu Shi, Weiqiang Tan, Zhonghua Zhao, Yanyong Xu, Huijuan Wu, Zhigang Zhang
Summary: UCHL1 deficiency leads to severe kidney damage and proteinuria in podocytes, which is associated with ER stress, protein accumulation, and cell apoptosis. UCHL1 may be a potential target for preventing non-immune complex-mediated glomerulopathy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Genetics & Heredity
Delfien Syx, Yoshihiro Ishikawa, Jan Gebauer, Sergei P. Boudko, Brecht Guillemyn, Tim Van Damme, Sanne D'hondt, Sofie Symoens, Sheela Nampoothiri, Douglas B. Gould, Ulrich Baumann, Hans Peter Bachinger, Fransiska Malfait
Summary: Osteogenesis imperfecta is caused by a homozygous p.(R222S) substitution in the HSP47 gene, resulting in significantly reduced affinity of HSP47-R222S for type I collagen and leading to overmodification of type I collagen. Unlike other types of OI, this overmodification is not due to prolonged exposure of collagen to modifying enzymes, as the intracellular folding rate of type I collagen appears to be normal. Abnormal binding of HSP47-R222S results in upregulation and increased binding of molecular chaperones and collagen-modifying enzymes, suggesting a compensatory mechanism for aberrant binding and eventually leading to overmodification of type I collagen chains.
Article
Biochemistry & Molecular Biology
Xueting Li, Ce Chen, Mingyue He, Lidong Yu, Renhao Liu, Chunmeng Ma, Yu Zhang, Jianbo Jia, Bingsheng Li, Li Li
Summary: Lead is an important raw material for industrial production, but it poses serious harm to aquatic ecosystems, especially causing spinal malformations in zebrafish embryos, which is mainly attributed to Pb2+-induced endoplasmic reticulum stress and apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Haiying Liu, Luguang Wang, Linyu Dai, Fumin Feng, Yonghong Xiao
Summary: The study found that the CaMK II/Ca2+ signaling pathway regulates the ERS apoptosis pathway and induces apoptosis in hepatic stellate cells (HSCs).
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Oncology
Yohei Yoshihama, Hidenori Namiki, Taiga Kato, Naomi Shimazaki, Sachiko Takaishi, Kumiko Kadoshima-Yamaoka, Hideo Yukinaga, Naoyuki Maeda, Tomoko Shibutani, Kazunori Fujimoto, Makoto Hirasawa, Hiroki Goto, Naoya Wada, Shinji Tsutsumi, Yasuhide Hirota, Tomoki Ishikawa, Shoji Yamamoto
Summary: This study reveals a specific dependency on PTDSS1 for phosphatidylserine synthesis following PTDSS2 deletion and introduces novel PTDSS1 inhibitors as a therapeutic option to induce collateral lethality in cancer with PTDSS2 loss.
Review
Cell Biology
Jody Groenendyk, Wen-An Wang, Alison Robinson, Marek Michalak
Summary: Calreticulin is a crucial protein for cardiac development and maintenance of proper cardiac function. It supports Ca2+-dependent signaling and enhances the efficiency of cardiomyocytes.
Article
Biochemistry & Molecular Biology
Vagner Ramon R. Silva, Angela Molinaro, Andrea Usseglio Gaudi, Emanuel Fryk, Claudia Sardi, Maria Hammarlund, Filip Mjornstedt, Maria E. Johansson, Barbara Becattini, Per-Anders Jansson, Giovanni Solinas
Summary: This study investigates the role of IKK beta signaling in obesity. The results show that the deletion of IKK beta in the liver and leukocytes is not tolerated in obese mice exposed to TLR3 agonists. However, selective hepatic IKK beta ablation improves fatty liver and insulin sensitivity.
Article
Agriculture, Dairy & Animal Science
Bingbing Ma, Tong Xing, Jiaolong Li, Lin Zhang, Yun Jiang, Feng Gao
Summary: This study investigated the mechanism of chronic heat stress-induced liver damage in broiler chickens. It found that chronic heat exposure caused liver microvesicular steatosis, vacuolar degeneration, and apoptosis in broilers. The study also observed increased liver relative weight, liver glutaminase activity, and plasma ammonia level in response to heat stress. Furthermore, heat stress enhanced the expression of endoplasmic reticulum stress response genes and apoptosis-related genes in the broiler liver.
Article
Medicine, General & Internal
Lei Zhang, Jieying Zeng, Huaiyu Wu, Hongtian Tian, Di Song, Weiqing Wu, Fajin Dong
Summary: The role and mechanism of TXNDC5 in hepatic fibrosis were explored in this study. The results showed that knockdown of TXNDC5 reduced hepatic fibrosis by regulating endoplasmic reticulum stress (ERs).
AMERICAN JOURNAL OF THE MEDICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ryo Ushioda, Akitoshi Miyamoto, Michio Inoue, Satoshi Watanabe, Masaki Okumura, Ken-ichi Maegawa, Kaiku Uegaki, Shohei Fujii, Yasuko Fukuda, Masataka Umitsu, Junichi Takagi, Kenji Inaba, Katsuhiko Mikoshiba, Kazuhiro Nagata
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biochemistry & Molecular Biology
Ken-ichi Maegawa, Satoshi Watanabe, Kentaro Noi, Masaki Okumura, Yuta Amagai, Michio Inoue, Ryo Ushioda, Kazuhiro Nagata, Teru Ogura, Kenji Inaba
Article
Hematology
P. Sasikumar, K. S. AlOuda, W. J. Kaiser, L. M. Holbrook, N. Kriek, A. J. Unsworth, A. P. Bye, T. Sage, R. Ushioda, K. Nagata, R. W. Farndale, J. M. Gibbins
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2018)
Article
Pathology
Yuki Kato, Yusaku Masago, Chiaki Kondo, Erika Yogo, Mikinori Torii, Atsuko Hishikawa, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate
TOXICOLOGIC PATHOLOGY
(2018)
Article
Cell Biology
Ryo Ushioda, Kazuhiro Nagata
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2019)
Article
Radiology, Nuclear Medicine & Medical Imaging
Tomomi Yamada, Yuto Kashiwagi, Takemi Rokugawa, Hideaki Kato, Haruyo Konishi, Tadateru Hamada, Ryohei Nagai, Yusaku Masago, Michiko Itoh, Takayoshi Suganami, Yoshihiro Ogawa, Kohji Abe
MAGNETIC RESONANCE IMAGING
(2019)
Article
Cell Biology
Michio Inoue, Nanami Sakuta, Satoshi Watanabe, Yuxia Zhang, Kunihito Yoshikaie, Yoshiki Tanaka, Ryo Ushioda, Yukinari Kato, Junichi Takagi, Tomoya Tsukazaki, Kazuhiro Nagata, Kenji Inaba
Article
Multidisciplinary Sciences
Riyuji Yamashita, Shohei Fujii, Ryo Ushioda, Kazuhiro Nagata
Summary: ERdj5 in the endoplasmic reticulum plays a crucial role in regulating intracellular calcium levels, affecting mitochondrial homeostasis and cellular senescence.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Hiroki Tanemura, Kenji Masuda, Takeshi Okumura, Eri Takagi, Daisuke Kajihara, Hirofumi Kakihara, Koichi Nonaka, Ryo Ushioda
Summary: In this study, a novel high-expression promoter was cloned based on transcriptome analysis to significantly enhance mAb production in CHO cells. The promoter showed improved productivity for various IgG subclasses and may be a new method for maintaining protein homeostasis and achieving stable expression of introduced mAb genes.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Yuka Adachi Katayama, Shin Hayase, Yoshinori Ando, Tomohiro Kuroita, Kazuya Okada, Ryo Iwamoto, Toru Yanagimoto, Masaaki Kitajima, Yusaku Masago
Summary: In this study, researchers developed an automated method called COPMAN that can concentrate and detect multiple types of viruses from small volume wastewater. The COPMAN showed high efficiency in detecting viruses from wastewater and successfully detected both spiked and indigenous SARS-CoV-2 in wastewater samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Yuka Adachi Katayama, Shin Hayase, Ryo Iwamoto, Tomohiro Kuroita, Tomohiko Okuda, Masaaki Kitajima, Yusaku Masago
Summary: Wastewater surveillance is important for understanding the prevalence of infectious diseases. This study improved the detection method for wastewater, allowing for simultaneous detection of multiple microorganisms, including SARS-CoV-2. The modified method showed enhanced detection and extraction efficiency, making it suitable for automated systems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Biochemistry & Molecular Biology
Cody Caba, Hyder Ali Khan, Janeen Auld, Ryo Ushioda, Kazutaka Araki, Kazuhiro Nagata, Bulent Mutus
FRONTIERS IN MOLECULAR BIOSCIENCES
(2018)
Article
Biochemical Research Methods
Kazutaka Araki, Ryo Ushioda, Hidewo Kusano, Riko Tanaka, Tomohisa Hatta, Kazuhiko Fukui, Kazuhiro Nagata, Tohru Natsume
ANALYTICAL BIOCHEMISTRY
(2017)