Article
Biochemistry & Molecular Biology
Emma Scott, Kirsty Hodgson, Beatriz Calle, Helen Turner, Kathleen Cheung, Abel Bermudez, Fernando Jose Garcia Marques, Hayley Pye, Edward Christopher Yo, Khirul Islam, Htoo Zarni Oo, Urszula L. McClurg, Laura Wilson, Huw Thomas, Fiona M. Frame, Margarita Orozco-Moreno, Kayla Bastian, Hector M. Arredondo, Chloe Roustan, Melissa Anne Gray, Lois Kelly, Aaron Tolson, Ellie Mellor, Gerald Hysenaj, Emily Archer Goode, Rebecca Garnham, Adam Duxfield, Susan Heavey, Urszula Stopka-Farooqui, Aiman Haider, Alex Freeman, Saurabh Singh, Edward W. Johnston, Shonit Punwani, Bridget Knight, Paul McCullagh, John McGrath, Malcolm Crundwell, Lorna Harries, Denisa Bogdan, Daniel Westaby, Gemma Fowler, Penny Flohr, Wei Yuan, Adam Sharp, Johann de Bono, Norman J. Maitland, Simon Wisnovsky, Carolyn R. Bertozzi, Rakesh Heer, Ramon Hurtado Guerrero, Mads Daugaard, Janne Leivo, Hayley Whitaker, Sharon Pitteri, Ning Wang, David J. Elliott, Benjamin Schumann, Jennifer Munkley
Summary: Prostate cancer is the leading cancer among men, causing over 350,000 deaths worldwide annually. This study reveals that the glycosyltransferase enzyme GALNT7 is upregulated in prostate cancer tissue and can be used as a biomarker to diagnose the disease with higher accuracy than the traditional PSA test. Additionally, GALNT7 is found to play a role in promoting prostate tumor growth and is associated with cell cycle and immune signaling pathways. Overall, this study highlights the importance of GALNT7-mediated O-glycosylation in prostate cancer progression.
Review
Cell Biology
Yuhan Zhang, Lingbo Sun, Changda Lei, Wenyan Li, Jiaqi Han, Jing Zhang, Yuecheng Zhang
Summary: This paper reviews the normal biosynthesis of mucin-type O-glycans and their role in maintaining body health. It also discusses the mechanisms of abnormal mucin-type O-glycosylation in disease development, especially cancer, with a specific focus on gastric cancer. Furthermore, tumor immunotherapy targeting mucin-type O-glycans is also discussed.
Review
Anatomy & Morphology
Kazuyoshi Itoh, Shoko Nishihara
Summary: Mucin-type O-glycosylation, a prominent type of glycosylation, is evolutionarily conserved and plays crucial physiological roles in animals. Studies in Drosophila have shown that these glycans are essential for CNS development, NMJ morphogenesis, and synaptic functions, providing insight into their functions in the mammalian brain.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Biophysics
Junqi Zhao, Jihong Wang, Yawen Liu, Xiao Xia Han, Baofeng Xu, Yukihiro Ozaki, Bing Zhao
Summary: In this study, a SERS-based aptasensor was developed to detect prostate-specific antigen biomarkers. With a specific design, the PSA levels in serum samples collected from prostate cancer patients were successfully determined.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Shan Huang, Kristina A. Thomsson, Chunsheng Jin, Henrik Ryberg, Nabangshu Das, Andre Struglics, Ola Rolfson, Lena I. Bjorkman, Thomas Eisler, Tannin A. Schmidt, Gregory D. Jay, Roman Krawetz, Niclas G. Karlsson
Summary: The study aimed to identify inflammatory markers related to systemic and local inflammation in osteoarthritis (OA). The researchers found 29 markers that were consistently detected in both plasma and synovial fluid, suggesting independent inflammatory environments in these two biofluids. They also discovered a positive correlation between the level of certain inflammatory markers in the synovial fluid and altered glycosylation of lubricin, indicating that truncated glycans of lubricin contribute to synovial proinflammatory cytokine production and exacerbate local synovial inflammation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Cancan Bao, Rui Zhang, Yanxia Qiao, Xiaowei Cao, Fangyuan He, Wenli Hu, Ming Wei, Wenbo Lu
Summary: A simple electrochemical immunosensor based on three-dimensional Au nanoparticles anchored on a cobalt boride nanowire array (AuNPs/CoB NWA) electrode is developed for the detection of prostate-specific antigen (PSA). An innovative signal amplification strategy is proposed without using the second labeled antibody to enhance sensitivity of the immunosensor. The electrocatalytic reduction of Nile blue A (NBA) is amplified on AuNPs/CoB NWAs, allowing for the accurate detection of PSA in human serum samples with high sensitivity and fine linearity.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Organic
Bozhena S. Komarova, Natalia S. Novikova, Alexey G. Gerbst, Olga A. Sinitsyna, Ekaterina A. Rubtsova, Elena G. Kondratyeva, Arkady P. Sinitsyn, Nikolay E. Nifantiev
Summary: The stereospecific α-glucosylation of primary and secondary OH groups at carbohydrate acceptors was achieved using the glucosyl N-phenyl-trifluoroacetimidate (PTFAI) donor protected with electron-withdrawing 2,4,5-trifluorobenzoyl (TFB) group at O-6 and the participating levulinoyl (Lev) group at O-3. The α-stereoselectivity in the case of TFB and pentafluorobenzoyl (PFB) groups at O-6 was attributed to conformational factors, as confirmed by DFT calculations. The potential of this donor, along with the orthogonality of TFB and Lev protecting groups, was demonstrated by the synthesis of α-(1→3)-linked pentaglucoside corresponding to Aspergillus fumigatus α-(1→3)-d-glucan and its hexasaccharide derivative with a β-glucosamine residue at the non-reducing end.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Organic
Tanmoy Halder, Somnath Yadav
Summary: The synthesis of the trisaccharide repeating unit of P. stuartii O49 serotype O-antigen polysaccharide has been successfully achieved, laying the foundation for the development of vaccines against this pathogen.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Oncology
Troels Boldt Romer, Mikkel Koed Moller Aasted, Sally Dabelsteen, Aaron Groen, Julia Schnabel, Edwin Tan, Johannes Wirenfeldt Pedersen, Amalie Dahl Haue, Hans Heugh Wandall
Summary: Novel immunotherapies targeting cancer-associated truncated O-glycans Tn and STn show promise in cancer treatment, with surface expression limited to cancers of epithelial origin. Tn and STn expression levels decrease with tumor grade, making them attractive immunological targets for human carcinomas. Patient-derived xenografts have shown to maintain Tn expression, highlighting the potential for targeting these glycan antigens in cancer therapy.
BRITISH JOURNAL OF CANCER
(2021)
Article
Chemistry, Analytical
Zhenping Liu, Qiuju Huang, Yu Yan, Jiyuan Yao, Fenqing Zhong, Shuting Xie, Minmin Zhang, Hua Zhang, Mingliang Jin, Lingling Shui
Summary: Significant progress has been made in recent decades, but the development of an accurate, portable, and high-throughput electrochemical biosensor for quantifying trace biomarkers in complex biological samples remains challenging. In this study, a novel portable integrated biosensor array was described. The biosensor array exhibited exceptional sensitivity and rapid detection of carbohydrate antigen 125 (CA-125) with a low detection limit and high throughput capability. This technology presents a promising strategy for detecting biomarkers in biological analysis and early disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Liang Yan, Shuai Xu, Fengna Xi
Summary: A sensitive electrochemical immunosensor was constructed for the detection of PSA based on amino-rich nanochannels array-modified disposable ITO electrodes. The use of NH2-VMSF as a recognitive interface enabled sensitive detection of PSA and achieved a LOD of 8.1 pg/mL. The proposed immunosensor offers a new idea for developing a universal immunosensing platform for sensitive detection of tumor markers.
Article
Radiology, Nuclear Medicine & Medical Imaging
Oliver Sartor, Christian la Fougere, Markus Essler, Samer Ezziddin, Gero Kramer, Joerg Ellinger, Luke Nordquist, John Sylvester, Giovanni Paganelli, Avivit Peer, Martin Boegemann, Jeffrey Meltzer, Per Sandstroem, Frank Verholen, Daniel Y. Song
Summary: The study analyzed the clinical outcomes of sequential alpha-/beta-emitter therapy in patients with metastatic castration-resistant prostate cancer (mCRPC). The results suggest that the use of Lu-177-PSMA after Ra-223 is feasible and may have potential benefits in terms of safety and overall survival. Further research is needed to confirm these findings.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Review
Chemistry, Multidisciplinary
Shuang Yue, Xiaotong Wang, Wei Ge, Jiajia Li, Chuanlai Yang, Zeyang Zhou, Peng Zhang, Xiaodong Yang, Wenjin Xiao, Shuang Yang
Summary: Mucin-type O-glycosylation is a crucial protein post-translational modification expressed abundantly on cell surface proteins. It plays various roles in cellular functions, such as protein structure, signal transduction, and immune response. Dysregulation of mucin O-glycosylation can impair mucosal protection against infection or immune evasion. Truncated O-glycosylation, known as Tn antigen or O-GalNAcylation, is upregulated in diseases including cancer, autoimmune disorders, neurodegenerative diseases, and IgA nephropathy. Analyzing and understanding O-GalNAcylation have important clinical implications, although it remains challenging due to the lack of reliable assays compared to N-glycosylation.
Article
Chemistry, Analytical
Suntisak Khumngern, Panote Thavarungkul, Proespichaya Kanatharana, Tanan Bejrananda, Apon Numnuam
Summary: In this study, an electrochemical sensor based on MIP was developed by modifying a screen-printed carbon electrode with PEDOT:PSS@Fc and a MIP layer. The sensor successfully detected the concentration of PSA.
MICROCHEMICAL JOURNAL
(2022)
Review
Oncology
E. J. Bass, A. Pantovic, M. Connor, R. Gabe, A. R. Padhani, A. Rockall, H. Sokhi, H. Tam, M. Winkler, H. U. Ahmed
Summary: Biparametric MRI shows comparable diagnostic performance to multiparametric MRI in detecting prostate cancer, but heterogeneity exists which prevents definitive recommendations from being made. Prospective multicentre studies are needed to further assess the efficacy of biparametric MRI in biopsy naive men.
PROSTATE CANCER AND PROSTATIC DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Kathrin Stavenhagen, Akul Y. Mehta, Lisa Laan, Chao Gao, Jamie Heimburg-Molinaro, Irma van Die, Richard D. Cummings
Summary: The macrophage mannose receptor (MR) is involved in immunity and maintaining homeostasis. The glycans within MR act as a regulatory switch by masking and unmasking self-ligands, controlling the receptor's activity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chuncui Huang, Meijie Hou, Jingyu Yan, Hui Wang, Yu Wang, Cuiyan Cao, Yaojun Wang, Huanyu Gao, Xinyue Ma, Yi Zheng, Dongbo Bu, Wengang Chai, Yan Li, Shiwei Sun
Summary: Accurate identification of glycan structures is challenging due to the existence of multiple isomeric structures in glycan samples. Researchers developed an intelligent group-opting strategy, named GIPS-mix, to assign isomeric glycans within mixtures based on simulated spectra and experimental spectra similarity. If a single group cannot be elected, a tie break is performed by additional MSn scanning using intelligently selected precursors. Through the application of GIPS-mix on standard mixtures and human milk oligosaccharide fractions, high accuracy and efficiency in assigning individual glycans in mixtures were demonstrated.
ANALYTICAL CHEMISTRY
(2023)
Article
Reproductive Biology
Kun Han, Yulu Yue, Weiwei Wang, Feiyu Wang, Wengang Chai, Shuhong Zhao, Mei Yu
Summary: Successful attachment of conceptus to the uterine luminal epithelium is crucial for establishing a functional placenta in pigs. Le(x)-carrying O-glycans might modulate conceptus attachment by interacting with the SPP1-integrin receptor complex. The B4GALT1 gene is a potential regulator for Le(x) antigen expression.
BIOLOGY OF REPRODUCTION
(2023)
Article
Chemistry, Applied
Sofia F. Reis, Susana Messias, Rita Bastos, Vitor J. Martins, Viviana G. Correia, Benedita A. Pinheiro, Lisete M. Silva, Angelina S. Palma, Manuel A. Coimbra, Elisabete Coelho
Summary: Brewing practice involves reusing the same yeast for subsequent fermentations. The yeast used for Lager beer (Saccharomyces pastorianus) is widely reused without changes in fermentation performance. However, the yeast used for Ale beer (Saccharomyces cerevisiae) is either partially or not reused due to poor performance. The study found that cells modulate their wall polysaccharides to increase cell wall strength.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Applied
Jiaqi Li, Ye Bi, Yi Zheng, Cuiyan Cao, Long Yu, Zhenyu Yang, Wengang Chai, Jingyu Yan, Jianqiang Lai, Xinmiao Liang
Summary: A high-throughput method was developed for the accurate quantitation of 23 complex human milk oligosaccharides. The method was fast and reliable, and was validated through a large population survey of mothers from different ethnic groups in China.
Article
Food Science & Technology
Sofia F. Reis, Vitor J. Martins, Rita Bastos, Tania Lima, Viviana G. Correia, Benedita A. Pinheiro, Lisete M. Silva, Angelina S. Palma, Paula Ferreira, Manuel Vilanova, Manuel A. Coimbra, Elisabete Coelho
Summary: This study aims to investigate the feasibility of brewer's spent yeast (BSY) microcapsules as oral carriers for food and biomedical applications. The results demonstrate that BSY microcapsules have strong resistance to digestion and are rich in (beta 1 -> 3)-glucans, which can be recognized by the human Dectin-1 immune receptor. Additionally, the digested material is recognized by various lectins related to (beta 1 -> 3)-glucans, glycogen, and mannans.
Article
Biochemistry & Molecular Biology
Peeyush N. Goel, Hongtao Zhang, Ramachandran Murali, Cai Zheng, Mei Q. Ji, Angelica Patterson, Payal Grover, Mark Greene
Summary: Mutations in the EGFR gene are common in NSCLC, with the L858R mutation in Exon 21 being the most prevalent. While targeted therapies like monoclonal antibodies and TKIs have shown efficacy, resistance eventually develops due to mutations such as T790M and C797S. A third-generation TKI, AZD9291, has high affinity for these mutations, but recent research suggests the emergence of resistance due to the EGFR (C797S) mutation. Additionally, erbB2 amplification is another significant mechanism of resistance. A small kinase inhibitor, ER121, has been developed to target these mutations and has shown promising inhibitory activity in mutant EGFR and amplified ErbB2 cancers.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Marie N. Sorin, Antonio Di Maio, Lisete M. Silva, Domenic Ebert, Clement P. Delannoy, Ngoc-Khan Nguyen, Yann Guerardel, Wengang Chai, Franck Halary, Karine Renaudin-Autain, Yan Liu, Celine Bressollette-Bodin, Thilo Stehle, Dorian McIlroy
Summary: This study characterizes the impact of naturally occurring VP1 mutations on ganglioside binding, VP1 protein structure, and virus tropism in BK polyomavirus (BKPyV). Mutants E73Q, E73A, and A72V-E73Q-E82Q (VQQ) retain sialic acid-dependent infectious entry and bind to a-series gangliosides, including GD1a. However, E73A and VQQ mutants lose the ability to infect ganglioside-complemented cells, and this correlates with a BC2 loop shift in their crystal structures. The K69N mutation in the K69N-E82Q variant inhibits sialic acid binding but retains significant infectivity in 293TT cells, suggesting the existence of an unknown sialic acid-independent entry receptor for BKPyV.
Article
Chemistry, Applied
Chuncui Huang, Yue Lu, Lin Kong, Zhendong Guo, Keli Zhao, Zheng Xiang, Xinyue Ma, Huanyu Gao, Yongfang Liu, Zhongmin Gao, Lijuan Xu, Wengang Chai, Yan Li, Yao Zhao
Summary: Human milk oligosaccharides (HMOs) are important for protecting infants from infections, especially for preterm infants. However, there is limited research on the specific differences in HMOs as a function of gestation time. In this study, we established an approach to analyze HMOs and found that the composition and relative abundance of HMOs changed with postpartum age, with significant differences in HMOs secreted at different postpartum times.
CARBOHYDRATE POLYMERS
(2023)
Meeting Abstract
Urology & Nephrology
Tohru Yoneyama, Shingo Hatakeyama, Mihoko Sutoh Yoneyama, Taku Yoshiya, Tsuyoshi Uemura, Takehiro Ishizu, Minoru Suzuki, Shingo Hachinohe, Shintaro Ishiyama, Motohiro Nonaka, Michiko N. Fukuda, Chikara Ohyama
JOURNAL OF UROLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
D. O. Ribeiro, B. A. Pinheiro, J. L. A. Bras, L. M. Silva, Y. Zhang, Y. Liu, T. Feizi, C. M. G. A. Fontes, A. L. Carvalho, W. Chai, A. S. Palma
Article
Biochemistry & Molecular Biology
Yukie Akune, Sena Arpinar, Lisete M. Silva, Angelina S. Palma, Virginia Tajadura-Ortega, Kiyoko F. Aoki-Kinoshita, Rene Ranzinger, Yan Liu, Ten Feizi
Summary: CarbArrayART is a software tool for glycan microarray data management, allowing storage, presentation, and export of data in compliance with the MIRAGE guidelines. It contributes to the evaluation of reproducibility and validity in glycobiology research.
Article
Biochemistry & Molecular Biology
Chunxia Li, Angelina S. Palma, Pengtao Zhang, Yibing Zhang, Chao Gao, Lisete M. Silva, Zhen Li, Filipa Trovao, Markus Weishaupt, Peter H. Seeberger, Leonid M. Likhosherstov, Vladimir Piskarev, Jin Yu, Ulrika Westerlind, Wengang Chai
Summary: Glycan microarrays are crucial for detecting and assigning specificity of glycan recognition by proteins, but current libraries are limited in size and diversity compared to estimated glycomes. Covalent and noncovalent immobilization are two common methods for microarray construction, and a direct comparison of results from these platforms is necessary.