Article
Biochemistry & Molecular Biology
Eshita Das, Shivcharan Prasad, Ipsita Roy
Summary: Peptidyl prolyl isomerases play a key role in accelerating protein folding, with larger multi-domain proteins having additional functions beyond isomerization. Yeast Fpr1 exhibits characteristics of a general chaperone in the proteostasis network, reducing protein aggregation and improving cell survival. Overexpression of Fpr1 protects cells against thermal shock, suggesting potential therapeutic implications in disease conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Cell Biology
Shannon McMahon, Steven Bergink, Harm H. Kampinga, Heath Ecroyd
Summary: The study shows that DNAJB proteins can inhibit protein aggregation by supporting the Hsp70-dependent degradation pathway, different from the mechanism of preventing fibrillar aggregation. The research also found that DNAJB6 and DNAJB8 have two distinct regions for binding substrates.
JOURNAL OF CELL SCIENCE
(2021)
Review
Neurosciences
Eiko N. Minakawa, Yoshitaka Nagai
Summary: Polyglutamine diseases are a group of inherited neurodegenerative disorders caused by abnormal expansion of CAG trinucleotide repeats. Stabilizing protein conformation and inhibiting aggregation offer promising therapeutic approaches, while gene silencing to reduce expression of expanded polyQ proteins is another potential treatment avenue. These advancements hold promise for delaying or preventing the progression of currently incurable diseases.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Uchu Matsuura, Shinya Tahara, Shinji Kajimoto, Takakazu Nakabayashi
Summary: Liquid-liquid phase separation leads to the formation of concentrated solute-containing droplets, which can aggregate and cause diseases. In this study, the protein aggregation process from the droplets was analyzed using autofluorescence lifetime microscopy, allowing for label-free protein structure analysis. The results showed that the droplet environment facilitates unique protein dynamics different from those in solutions.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Sai S. Pradhan, Sai M. Thota, Saiswaroop Rajaratnam, Sai K. S. Bhagavatham, Sujith K. Pulukool, Sriram Rathnakumar, Kanikaram S. Phalguna, Rajesh B. Dandamudi, Ashish Pargaonkar, Prasanth Joseph, E. Joshy, Venketesh Sivaramakrishnan
Summary: This study analyzed metabolomics data from HD patients and a yeast model of HD, and found a considerable overlap of deregulated metabolic pathways. The multiomics analysis also revealed common and unique deregulated pathways in human, mice, and yeast model systems. The modulation of these deregulated pathways was shown to influence protein aggregation in HD through the addition of certain metabolites and gene knockouts in the yeast model system. These findings highlight the importance of regulating deregulated pathways in HD for protein aggregation, disease progression, and prognosis.
DISEASE MODELS & MECHANISMS
(2022)
Review
Biochemistry & Molecular Biology
Ekambaram Srinivasan, Vavish Ram, Ramalingam Rajasekaran
Summary: Huntington's disease is a debilitating neurodegenerative disease caused by CAG repeat expansion in the huntingtin gene, leading to behavioral changes, motor dysfunction, and dementia in children and adults. Mutations in the huntingtin protein have been implicated in neuron loss through various mechanisms, and current research is focused on reducing huntingtin gene expression, protein accumulation, and aggregation as potential therapeutic strategies for HD.
CURRENT DRUG METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Fan Jiang, Guofeng Chang, Zhenzhen Li, Mostafa Abouzaid, Xiaoyong Du, J. Joe Hull, Weihua Ma, Yongjun Lin
Summary: This study used bioinformatics approaches to evaluate the transcript levels of heat shock protein network in Chilo suppressalis under cold-induced stress. It identified highly cold-inducible proteins in C. suppressalis and found differences in transcriptional regulation between cold-acclimation and short-term cold-shock. The activation of HSP/co-chaperone network in overwintering larvae depends on their diapause state, and cold acclimation can enhance their cold tolerance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Neurosciences
Gouri Chopra, Shabnam Shabir, Sumaira Yousuf, Simran Kauts, Shahnawaz A. Bhat, Ashiq H. Mir, Mahendra P. Singh
Summary: Neurodegenerative diseases are a cluster of diseases characterized by progressive neuronal loss and protein misfolding and aggregation. Recent studies have found that these misfolded proteins can translocate from one neuron to another, which has implications for understanding the onset and progression of the diseases and developing innovative therapies and diagnostics.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biophysics
Sharon E. Groover, Adewale Adegbuyiro, Caleb K. Fan, Breanna L. Hodges, Maryssa Beasley, Katelyn Taylor, Alyssa R. Stonebraker, Chathuranga Siriwardhana, Justin Legleiter
Summary: This study investigated the impact of macromolecular crowding on the aggregation of Huntington's disease-related protein htt in bulk solution and at interfaces. It was found that crowding agents influenced the aggregation characteristics of htt on different interfaces, highlighting the importance of crowding in the aqueous phase on this process.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Kevin Reinle, Axel Mogk, Bernd Bukau
Summary: The protein quality control system maintains protein homeostasis through substrate degradation, refolding, and sequestration activities, in which small heat shock proteins play a crucial role and adapt to different tasks and stress conditions.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Evren Onay Ucar, Aslihan Sengelen, Elif Mertoglu Kamali
Summary: The study reveals that heat shock response (HSR) is closely related to therapeutic resistance of gliomas. Resveratrol (RSV) shows potential as an experimental agent for glioblastoma (GB) therapy, but the role of heat shock proteins (Hsps) in RSV efficacy remains unclear. The findings suggest that silencing Hsp27, Hsp60, Hsp70, and Hsp90 makes glioma cells more sensitive to RSV treatment, making these Hsps potential therapeutic targets for GB treatment.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Anastasia V. Poznyak, Varvara A. Orekhova, Vasily N. Sukhorukov, Victoria A. Khotina, Mikhail A. Popov, Alexander N. Orekhov
Summary: Atherosclerosis is a global health problem, leading to increased morbidity and mortality from cardiovascular diseases. Effective measures for prevention and treatment have not been developed yet. Understanding molecular interactions and the role of heat shock proteins in the progression of atherosclerosis is crucial. Some heat shock proteins have antiatherosclerotic effects, while others' roles are unclear. This review focuses on the antiatherosclerotic activity of specific heat shock proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yan Zhu, Lukman O. Afolabi, Xiaochun Wan, Joong Sup Shim, Liang Chen
Summary: Neurodegenerative diseases are characterized by the degeneration of the central or peripheral nervous systems and the aggregation of misfolded proteins, leading to cellular dysfunction and brain damage. TRIM proteins play important roles in maintaining protein quality control and clearing misfolded protein aggregates associated with neurodegenerative diseases.
Article
Multidisciplinary Sciences
Ryuji Owada, Shinichi Mitsui, Kazuhiro Nakamura
Summary: The study revealed that aggregated polyS and polyL peptides can spontaneously enter neuron-like cells and astrocytes, causing cell degeneration, aberrant differentiation, and cell death. Injecting these aggregates into the ventricles of adult mice resulted in behavioral changes, with polyS injection leading to extensive vacuolar degeneration in the brain. This suggests that RAN translation-related proteins containing polyS and polyL have the potential to propagate and exert universal toxicity in recipient cells.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Rohan Gupta, Mehar Sahu, Devesh Srivastava, Swati Tiwari, Rashmi K. Ambasta, Pravir Kumar
Summary: Post-translational modifications play a crucial role in the pathogenesis of neurodegenerative diseases by regulating protein homeostasis and signaling cascades, leading to enhanced neuroprotection and potentially reversing misfolded protein accumulation.
AGEING RESEARCH REVIEWS
(2021)
Article
Biophysics
Amulya Nidhi Shrivastava, Luc Bousset, Marianne Renner, Virginie Redeker, Jimmy Savistchenko, Antoine Triller, Ronald Melki
BIOPHYSICAL JOURNAL
(2020)
Article
Clinical Neurology
Anke Van der Perren, Geraldine Gelders, Alexis Fenyi, Luc Bousset, Filipa Brit, Wouter Peelaerts, Chris Van den Haute, Steve Gentleman, Ronald Melki, Veerle Baekelandt
ACTA NEUROPATHOLOGICA
(2020)
Article
Biochemistry & Molecular Biology
Laurent Brasseur, Audrey Coens, Jehan Waeytens, Ronald Melki, Luc Bousset
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Eliana Nachman, Anne S. Wentink, Karine Madiona, Luc Bousset, Taxiarchis Katsinelos, Kieren Allinson, Harm Kampinga, William A. McEwan, Thomas R. Jahn, Ronald Melki, Axel Mogk, Bernd Bukau, Carmen Nussbaum-Krammer
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Josquin Courte, Luc Bousset, Ysander Von Boxberg, Catherine Villard, Ronald Melki, Jean-Michel Peyrin
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Maya Bendifallah, Virginie Redeker, Elodie Monsellier, Luc Bousset, Tracy Bellande, Ronald Melki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Luc Bousset, Nina Luckgei, Mehdi Kabani, Carole Gardiennet, Anne K. Schutz, Ronald Melki, Beat H. Meier, Anja Bockmann
FRONTIERS IN MOLECULAR BIOSCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Marina Pantazopoulou, Viviana Brembati, Angeliki Kanellidi, Luc Bousset, Ronald Melki, Leonidas Stefanis
Summary: The study explores the degradation mechanisms of abnormal protein aggregates in Parkinson's disease, suggesting that autophagy is the main pathway for clearing fibrillar alpha-Syn, while the proteasome system plays a role in selectively clearing phosphorylated alpha-Syn oligomers.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Maud Landureau, Virginie Redeker, Tracy Bellande, Stephanie Eyquem, Ronald Melki
Summary: The differential surface exposure of amino acids on distinct aSYN fibrillar polymorphs may contribute to their binding with partner proteins. These findings liken the polypeptides exposed on the surfaces of different aSYN fibrillar polymorphs to fingerprints, suggesting diagnostic and therapeutic potential.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Kaspar Russ, Gabriel Teku, Luc Bousset, Virginie Redeker, Sara Piel, Ekaterina Savchenko, Yuriy Pomeshchik, Jimmy Savistchenko, Tina C. Stummann, Carla Azevedo, Anna Collin, Stefano Goldwurm, Karina Fog, Eskil Elmer, Mauno Vihinen, Ronald Melki, Laurent Roybon
Summary: TNF-alpha and alpha-synuclein fibrils induce distinct immune reactive responses in astrocytes, accompanied by impaired mitochondrial respiration, with competition between the two stimuli. This study provides evidence for astrocytic involvement in Parkinson's disease pathology and highlights the complex immune reactive responses of astrocytes to exogenous stressors.
Article
Biochemistry & Molecular Biology
Emilie Caroux, Virginie Redeker, Karine Madiona, Ronald Melki
Summary: This study utilized proteomic approaches to map the differences in the atomic structure and exposed amino acid segments of tau filaments from different neurodegenerative diseases, providing insights for the design of specific ligands with diagnostic and therapeutic potential.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Clara Lejeune, Laila Sago, David Cornu, Virginie Redeker, Marie-Joelle Virolle
Summary: In most Streptomyces species, antibiotic production is repressed in phosphate proficiency, but the model strain S. coelicolor produces the polyketide antibiotic ACT even more abundantly in phosphate proficiency. The closely related strain S. lividans does not produce ACT in any phosphate condition, but its pptA mutant produces ACT in phosphate limitation. The common feature triggering ACT biosynthesis in S. coelicolor and the pptA mutant of S. lividans is the high oxidative stress.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Josquin Courte, Ngoc Anh Le, Teng Pan, Luc Bousset, Ronald Melki, Catherine Villard, Jean-Michel Peyrin
Summary: This study demonstrates the limited spreading of preformed aSyn aggregates and suggests that it occurs through molecular sieving of large aSyn seeds. The research also shows that synaptic connections do not facilitate this process. The development of a new microfluidic platform allows for the reconstruction of fully oriented neuronal networks in vitro, enabling the quantification of fluorescent aSyn aggregates spreading between neurons.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)