Review
Plant Sciences
Bing Yang, Chenyun Xu, Yuting Cheng, Ting Jia, Xueyun Hu
Summary: Iron-sulfur (Fe-S) clusters are ancient protein cofactors that exist ubiquitously in organisms and play important roles in various life processes. Plastids, semi-autonomous organelles believed to originate from cyanobacterial endosymbionts, have a Fe-S cluster biosynthesis and delivery pathway similar to cyanobacteria. Fe-S clusters are crucial for the normal functioning of downstream Fe-S proteins in plastids. Recent research has made significant progress in understanding this pathway and this review summarizes these findings as well as the remaining scientific challenges.
PLANT CELL REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Shinya Sugimoto, Kunitoshi Yamanaka, Tatsuya Niwa, Yurika Terasawa, Yuki Kinjo, Yoshimitsu Mizunoe, Teru Ogura
Summary: This study demonstrated the role of JDPs in bacterial survival during heat stress and the formation of curli-extracellular amyloid fibers. Genetic analysis revealed the essential role of DnaJ in survival at high temperature and the ability of either DnaJ or CbpA to activate curli production. Biochemical analyses showed that DnaJ and CbpA are involved in regulating the master regulator CsgD and maintaining CsgA in a translocation-competent state.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Genetics & Heredity
Lu Zhao, Ting Jia, Qingsong Jiao, Xueyun Hu
Summary: J-proteins, a type of molecular chaperone, play important roles in chloroplast development, photosynthesis, seed germination, and stress response in plants.
Article
Biochemistry & Molecular Biology
Felipe A. Carvalho, Ulrich Muehlenhoff, Joseph J. Braymer, Vasilij Root, Martin Stuempfig, Carla C. Oliveira, Roland Lill
Summary: Systematic studies have shown that the Hsp90 chaperone system and Fe/S protein biogenesis or iron regulation are interconnected. DJA5 and DJA6 are two chloroplast-localized proteins that act as iron donors in plastidial Fe/S protein biogenesis. In this study, the impact of Hsp90 chaperone, yeast DJA5-DJA6 homologs, cytosolic Ydj1, and mitochondrial Mdj1 on cellular iron-related processes was investigated using Saccharomyces cerevisiae. Depletion of these crucial proteins resulted in severe phenotypes, but did not have a critical in vivo impact on Fe/S protein biogenesis or iron regulation. Unlike plant DJA5-DJA6 iron chaperones, Ydj1 and Mdj1 did not bind iron in vivo, suggesting that zinc is used for their function under normal physiological conditions.
Article
Biochemistry & Molecular Biology
Dafne Suraci, Giovanni Saudino, Veronica Nasta, Simone Ciofi-Baffoni, Lucia Banci
Summary: The study reveals the coordinated mechanism of ISCA1, ISCA2, and NFU1 in the maturation process of mitochondrial [4Fe-4S] proteins. ISCA1 plays a key role in promoting the interaction between NFU1 and ISCA2, leading to the formation of a transient ISCA1-ISCA2-NFU1 ternary complex. This mechanism ensures the safe transfer of [4Fe-4S] clusters from the assembly site on the ISCA1-ISCA2 complex to NFU1, making them available for mitochondrial apo proteins that require NFU1.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Clinical Neurology
Conrad C. Weihl, Ana Topf, Rocio Bengoechea, Jennifer Duff, Richard Charlton, Solange Kapetanovic Garcia, Cristina Dominguez-Gonzalez, Abdulaziz Alsaman, Aurelio Hernandez-Lain, Luis Varona Franco, Monica Elizabeth Ponce Sanchez, Sarah J. Beecroft, Hayley Goullee, Jil Daw, Ankan Bhadra, Heather True, Michio Inoue, Andrew R. Findlay, Nigel Laing, Montse Olive, Gianina Ravenscroft, Volker Straub
Summary: This article reports a novel chaperonopathy associated with the DNAJB4 gene, causing muscle weakness and early respiratory failure. Loss of DNAJB4 function may lead to the accumulation of its client proteins, resulting in muscle dysfunction and degeneration.
ACTA NEUROPATHOLOGICA
(2023)
Article
Chemistry, Multidisciplinary
Giovanni Saudino, Simone Ciofi-Baffoni, Lucia Banci
Summary: In this study, it was discovered that NFU1 and ISCA1 in the mitochondrial iron-sulfur cluster assembly machinery play a crucial role in the insertion of a [4Fe-4S] cluster into LIAS by forming a heterodimeric complex. The C-domain of NFU1 drives the cluster to its final destination by exploiting a protein interaction affinity gradient.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Rashmi Yadav, Shamulailatpam Shreedarshanee Devi, Jyoti Oswalia, Sivaprakash Ramalingam, Ranjana Arya
Summary: Limited treatment options and research in understanding rare diseases like GNE Myopathy has raised concerns about therapeutic development. The study focuses on the mechanism of protein aggregate formation in GNE mutant cells, revealing HSP70 as a promising therapeutic target in reducing protein aggregation through activators like BGP-15.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sakiko Sato, Yumeka Matsushima, Miaki Kanazawa, Naoyuki Tanaka, Takashi Fujishiro, Kouhei Kunichika, Ryosuke Nakamura, Hiroaki Tomioka, Kei Wada, Yasuhiro Takahashi
Summary: IscU plays a central role in Fe-S cluster assembly and transfer, with potential for bypassing the essential roles of HscA and HscB through conformational interconversion. The study reveals dynamic conformational changes in IscU, highlighting the fundamental requirement for Fe-S cluster assembly and transfer.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jing Du, Zhaoyang Huang, Yanchun Li, Xueying Ren, Chaoting Zhou, Ruolan Liu, Ping Zhang, Guojie Lei, Jianxin Lyu, Jianghui Li, Guoqiang Tan
Summary: Copper is a necessary mineral nutrient but excess copper is cytotoxic. Wilson's disease is an autosomal recessive hereditary disease characterized by pathological copper accumulation in organs. The molecular mechanism of Wilson's disease is not fully understood and further research is needed to develop therapeutic strategies.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Maria Florencia Pignataro, Maria Georgina Herrera, Natalia Brenda Fernandez, Martin Aran, Hernan Gustavo Gentili, Fernando Battaglini, Javier Santos
Summary: In this study, a small protein, Affi_224, was successfully screened using ribosome display method. Affi_224 showed a high affinity for Frataxin and was able to increase the activity of Cys NFS1 desulfurase caused by the FRDA Frataxin variant G130V. The interaction between Affi_224 and Frataxin was also observed in a human cellular model. This research suggests that quaternary addition could be a new tool for modulating Frataxin function.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Lucia D. Espeche, Karl Ellioth Sewell, Ignacio H. Castro, Luciana Capece, Maria Florencia Pignataro, Liliana Dain, Javier Santos
Summary: The study found that replacement of frataxin by glycine at different tryptophan sites resulted in varying effects on iron-sulfur cluster assembly activity, with W155G showing no activity, W168G being fully active, and W173G having significantly decreased activity. Additionally, W173G may exhibit altered motions at the Trp155 site.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang
Summary: Fe-S clusters are ancient and important for life processes. The biogenesis of Fe-S clusters involves iron acquisition, sulfur mobilization, and cluster formation. Two Arabidopsis DnaJ proteins, DJA6 and DJA5, play a crucial role in facilitating iron incorporation into Fe-S clusters during chloroplast Fe-S cluster biogenesis. Loss of these proteins leads to defects in chloroplast Fe-S protein accumulation and photosynthesis dysfunction. Evolutionary analysis shows that DJA6 and DJA5 are highly conserved in photosynthetic organisms and have a strong evolutionary relationship with SUFE1, SUFC, and SUFD.
Review
Chemistry, Inorganic & Nuclear
Jason C. Crack, Nick E. Le Brun
Summary: Iron-sulfur (FeS) clusters are protein cofactors essential for various biological functions such as electron transfer, catalysis, and gene regulation. Recent progress in using mass spectrometry has provided new insights into FeS cluster assembly and chemistry, particularly in transcriptional regulators coordinating cellular responses to changing conditions.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Oncology
Jaewang Lee, Jong-Lyel Roh
Summary: Iron dysregulation is a characteristic of cancer, characterized by increased expression of genes involved in iron metabolism and iron-sulfur cluster (ISC) biogenesis. Dysregulated iron homeostasis leads to elevated intracellular labile iron, which can result in the formation of excessive cytotoxic radicals and susceptibility to regulated cell death, including ferroptosis. Various strategies, such as redox operations, iron chelation, and replacement of iron with redox-inert metals, can destabilize or restrict ISC formation and function, providing potential therapeutic approaches for cancer treatment. Targeting ISCs to induce ferroptosis shows promise in cancer therapy. This review provides a comprehensive overview of iron metabolism, ferroptosis, and the role of ISC modulation in cancer cells, as well as the potential of targeting ISCs for ferroptosis induction in cancer treatment. Further research and clinical trials are needed to validate these strategies for various cancers, with the goal of developing novel and effective cancer treatments.
Article
Biochemistry & Molecular Biology
Shubhi Srivastava, Mohammad Azharuddin Savanur, Devanjan Sinha, Abhijit Birje, R. Vigneshwaran, Prasenjit Prasad Saha, Patrick D'Silva
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Cell Biology
Gautam Pareek, Ruth E. Thomas, Leo J. Pallanck
CELL DEATH & DISEASE
(2018)
Editorial Material
Cell Biology
Gautam Pareek, Leo J. Pallanck
CELL DEATH & DISEASE
(2018)
Article
Biochemistry & Molecular Biology
Ina Nemet, Prasenjit Prasad Saha, Nilaksh Gupta, Weifei Zhu, Kymberleigh A. Romano, Sarah M. Skye, Tomas Cajka, Maradumane L. Mohan, Lin Li, Yuping Wu, Masanori Funabashi, Amanda E. Ramer-Tait, Sathyamangla Venkata Naga Prasad, Oliver Fiehn, Federico E. Rey, W. H. Wilson Tang, Michael A. Fischbach, Joseph A. DiDonato, Stanley L. Hazen
Article
Genetics & Heredity
Gautam Pareek, Leo J. Pallanck
Article
Cell Biology
Maradumane L. Mohan, Yuji Nagatomo, Prasenjit Prasad Saha, Sromona D. Mukherjee, Timothy Engelman, Rommel Morales, Stanley L. Hazen, W. H. Wilson Tang, Sathyamangla V. Naga Prasad
Summary: Dysregulation of immune responses can lead to the generation of IgG autoantibodies targeting human beta 1ARs, which contribute to negative cardiac outcomes. However, beta-blockers have shown benefits in patients with IgG3 autoantibodies, suggesting a potential role of these autoantibodies in altering beta 1AR function. Further investigation revealed that IgG3 autoantibodies bias beta-blockers towards G-protein coupling and alter signaling, leading to beneficial outcomes in patients.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Multidisciplinary Sciences
Khuraijam Dhanachandra Singh, Zaira P. Jara, Terri Harford, Prasenjit Prasad Saha, Triveni R. Pardhi, Russell Desnoyer, Sadashiva S. Karnik
Summary: This study reports the discovery of allosteric ligands for the AT1R receptor and their potential application in blocking autoimmune antibodies. Through molecular dynamics simulation and high-throughput virtual screening, compounds with a negative allosteric modulator effect on receptor function were identified, demonstrating efficacy in vivo.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cardiac & Cardiovascular Systems
Kymberleigh A. Romano, Ina Nemet, Prasenjit Prasad Saha, Arash Haghikia, Xinmin S. Li, Maradumane L. Mohan, Beth Lovano, Laurie Castel, Marco Witkowski, Jennifer A. Buffa, Yu Sun, Lin Li, Christopher M. Menge, Ilja Demuth, Maximilian Koenig, Elisabeth Steinhagen-Thiessen, Joseph A. DiDonato, Arjun Deb, Fredrik Baeckhed, W. H. Wilson Tang, Sathyamangla Venkata Naga Prasad, Ulf Landmesser, David R. Van Wagoner, Stanley L. Hazen
Summary: A study found that the gut microbiota-dependent metabolite PAGln is associated with the presence and severity of heart failure both clinically and mechanistically. The circulating levels of PAGln are dose-dependently correlated with heart failure presence and indices of severity. It was also found that PAGln directly promotes heart failure-related phenotypes. Modulating the gut microbiome and PAGln production may be a potential therapeutic target for heart failure.
CIRCULATION-HEART FAILURE
(2023)
Article
Multidisciplinary Sciences
Gautam Pareek
Summary: Mitochondria play crucial roles in cellular functions but can be damaged by reactive oxygen species and misfolded proteins, leading to aging and various diseases. Cells utilize mitochondrial-resident proteases for quality control to combat mitochondrial damage.
Article
Biochemistry & Molecular Biology
Manjunath P. Eelager, Saraswati P. Masti, Ravindra B. Chougale, Vishram D. Hiremani, Shivayogi S. Narasgoudar, Nagarjun Prakash Dalbanjan, S. K. Praveen Kumar
Summary: Vanillic acid incorporated chitosan/poly(vinyl alcohol) active films were prepared through a cost-effective solvent casting technique. The addition of vanillic acid improved the morphology and increased the tensile strength of the films. These active films showed excellent UV, water, and oxygen barrier properties, as well as antimicrobial activity and antioxidant capacity. Furthermore, they successfully prolonged the shelf life of green chillies compared to conventional polyethylene packaging.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Nagashree U. Hebbar, Prateeksha Subray Hebbar, Pooja Prakash Rathode, Beerappa Negalura, Aishwarya Hiremath, Parashuram Gudimani, Lokesh A. Shastri, S. K. Praveen Kumar, Arihant Jayawant Kadapure, Shrinivas Joshi
Summary: In this study, triazolidine-3-thione substituted butanoic acid and butanoate derivatives were synthesized as a tactic to combat antibiotic resistance. The synthesized moieties were characterized using IR, NMR, and LC-MS spectroscopy, and their in vitro antimicrobial activity was evaluated. Some of the molecules showed promising results against C. albicans, with lower minimum inhibitory concentration (MIC). Additionally, the compounds exhibited strong DPPH radical scavenging ability and low cytotoxicity against HeLa cells. Molecular docking study revealed excellent interaction between the molecules and the active site of Staph gyrase B enzyme.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Biochemistry & Molecular Biology
Marco Witkowski, Ina Nemet, Hassan Alamri, Jennifer Wilcox, Nilaksh Gupta, Nisreen Nimer, Arash Haghikia, Xinmin S. Li, Yuping Wu, Prasenjit Prasad Saha, Ilja Demuth, Maximilian Koenig, Elisabeth Steinhagen-Thiessen, Tomas Cajka, Oliver Fiehn, Ulf Landmesser, W. H. Wilson Tang, Stanley L. Hazen
Summary: Artificial sweeteners, especially erythritol, are associated with increased risk of major adverse cardiovascular events (MACE) and enhanced thrombosis formation. Ingestion of erythritol leads to elevated levels of erythritol in plasma, which is linked to heightened platelet reactivity and thrombosis potential. Further studies are needed to assess the long-term safety of erythritol.
Article
Chemistry, Multidisciplinary
Lokesh Bheemayya, Ravindra R. Kamble, Madivalagouda S. Sannaikar, Sanjeev R. Inamdar, Tukaram V. Metre, Barnabas Kodasi, Swati R. Hoolageri, Vishwa B. Nadoni, Shrinivas D. Joshi, S. K. Praveen Kumar, Nagarjuna Prakash Dalbanjan, K. M. Mussuvir Pasha, Rangappa S. Keri
Summary: In this study, Fe3O4 nanoparticles were prepared and used as efficient nanocatalysts for the synthesis of novel heterocycles. Optical, molecular docking, and antimicrobial studies were conducted, showing the compounds' fluorescent properties, strong binding affinities, and remarkable antimicrobial activity.
Article
Cell Biology
Gautam Pareek, Ruth E. Thomas, Evelyn S. Vincow, David R. Morris, Leo J. Pallanck
CELL DEATH DISCOVERY
(2018)
Review
Multidisciplinary Sciences
Devanjan Sinha
PROCEEDINGS OF THE INDIAN NATIONAL SCIENCE ACADEMY
(2017)