Article
Environmental Sciences
Ambika H. Shinde, Ashwini Sharma, Saksham Doshi, Madhava Anil Kumar, Soumya Haldar
Summary: This study isolated 201 native and indigenous probiotic candidates and selected 19 sulfur-oxidizing bacteria as part of a probiotic consortium for remediating reduced sulfur in aquaculture and coastal environments.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Genetics & Heredity
Ana Ibanez, Sonia Garrido-Chamorro, Juan J. R. Coque, Carlos Barreiro
Summary: This review summarizes the latest research progress on sulfur metabolic processes in Acidithiobacillus species, which play a crucial role in industrial biomining processes. The challenges and barriers to further study the sulfur metabolism of this peculiar extremophilic genus are also highlighted.
Article
Microbiology
Yang He, Xiang Zeng, Fei Xu, Zongze Shao
Summary: A total of 17 mixotrophic neutrophilic thiosulfate- and iron-oxidizing bacteria were isolated from hydrothermal fields on the Carlsberg Ridge in the Indian Ocean. These bacteria belonged to different taxonomic groups, including gamma-proteobacteria, alpha-proteobacteria, and Actinobacteria. Physiological characterization revealed their versatility in electron donors and various lifestyles. The representative strain, Pseudomonas sp. IOP_13, showed autotrophic growth, enhanced carbon dioxide fixation, increased thiosulfate-oxidizing ability, and decreased iron-oxidizing ability.
Article
Microbiology
Tongchu Deng, Zhili He, Meiying Xu, Meijun Dong, Jun Guo, Guoping Sun, Haobin Huang
Summary: This study demonstrates how species' functional traits and interactions determine microbial community structure and functions, further revealing the mechanisms governing the assembly and functions of nitrate-mediated sulfur oxidation microbiomes. It also has important implications for designing and constructing microbiomes with expected functions.
Article
Environmental Sciences
Lichang Zhou, Zhaoling Li, Boyi Cheng, Jinqi Jiang, Xinqi Bi, Zongping Wang, Guanghao Chen, Gang Guo
Summary: Thiosulfate can promote sulfur-mediated bacterial activity, inhibit glycogen accumulating organisms, and enhance denitrification efficiency. After the carbon source is reduced, the competitive ability of glycogen accumulating organisms increases, resulting in reduced sulfate reduction.
ENVIRONMENTAL RESEARCH
(2024)
Article
Biotechnology & Applied Microbiology
Syed Nyamath, Karthikeyan Subburamu, Geetha Thanuja Kalyanasundaram, Dananjeyan Balachandar, Meena Suresh, Rangasamy Anandham
Summary: In this study, five sulfur-oxidizing bacteria (SOB) isolates from rice rhizosphere were identified and characterized. These isolates showed the ability to oxidize reduced sulfur compounds to sulfate and also possessed beneficial properties such as phosphate and zinc solubilization, acetic acid and indole acetic acid production, and ACC deaminase activity.
FOLIA MICROBIOLOGICA
(2023)
Article
Engineering, Environmental
Huiling Zeng, Hailan Chen, Bing Yang, Junyi Zeng, Lin Meng, Donglin Shi, Liang Chen, Youju Huang
Summary: A highly-oxidizing Au@MnO2-X nanozyme is designed, which catalyzes oxidation through electron transfer instead of O-2-mediated superoxide anion process. Based on this specially structured nanozyme, an oxygen independent and ultrasensitive nanozyme-based sensor is established, achieving Ops detection at a LOD of 0.039 ng mL(-1).
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Linpeng Yu, Dan He, Eryi Zhang, Qiuxiang He, Jibing Li, Zhiyong Jason Ren, Shungui Zhou
Summary: This study demonstrates the electricity generation using Anaerobic oxidation of methane (AOM) driven by the single methanogen Methanosarcina barkeri, revealing its electrogenic mechanism. The findings suggest that M. barkeri can directly donate electrons to electrodes and secrete redox-active compounds to facilitate indirect extracellular electron transfer (EET).
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Abhiney Jain, Anaisa Coelho, Joana Madjarov, Catarina M. Paquete, Jeffrey A. Gralnick
Summary: Fe(II)-oxidizing bacteria are crucial in biogeochemical cycles and perform extracellular electron transfer through the Mto pathway. The electron transport proteins required for the growth of Sideroxydans species remain understudied, and there is debate about the functionality and directionality of these proteins.
Article
Microbiology
Shaobin Xie, Shasha Wang, Dengfeng Li, Zongze Shao, Qiliang Lai, Yejian Wang, Mingcong Wei, Xiqiu Han, Lijing Jiang
Summary: A novel mesophilic bacterium capable of hydrogen and sulfur oxidation, designated strain ST-419(T), was isolated from a deep-sea hydrothermal vent. It can grow at a wide range of temperature, pH, and salinity levels using various sources of energy. Phylogenetic analysis based on 16S rRNA gene sequences and physiological characteristics confirmed the bacterium as a new species within the genus Sulfurovum.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Chemistry, Organic
Xunbo Lu, Guoling Huang, Jianlin Ye, Muhammad Adnan Bashir, Jianfang Su, Kaiyuan Yang, Fangpeng Liang, Xinyu Xu
Summary: In this study, a novel and efficient approach for the oxidative esterification of sulfenamides using phenyliodonium diacetate is presented. The method enables the synthesis of sulfinimidate esters and sulfilimines under mild and metal-free conditions, with yields up to 99%. The protocol is scalable and compatible with a diverse range of substrates and functional groups, showing potential for late-stage functionalization of pharmacologically relevant molecules. Furthermore, a plausible reaction mechanism is proposed to explain the observed sequence of events.
Article
Microbiology
Mehdi Alibaglouei, Leah R. Trutschel, Annette R. Rowe, Joshua D. Sackett
Summary: The full genome sequence of Halomonas sp. M1, isolated from a high pH serpentinizing spring in northern California, USA, is reported. The 3.7 Mb genome has a high G + C content of 54.13% and encodes 3,354 protein-coding genes, providing insights into the metabolic potential for sulfur oxidation.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Chemistry, Organic
Guoling Huang, Jianlin Ye, Muhammad Adnan Bashir, Yuetong Chen, Wenjing Chen, Xunbo Lu
Summary: In this study, a novel and efficient method for the oxidativeamination reaction of sulfenamides was developed using diacetoxyiodobenzene and amines under basic conditions. This technique simplifies the synthesis of sulfinamidines, achieving high yields up to 99% without the need for metal catalysts. Possible reaction pathways were proposed to explain the observed molecular sequence of events. This cutting-edge approach not only advances the synthesis of valuable sulfinamidine compounds, but also expands the synthetic toolbox available to chemists, paving the way for future discoveries in organic synthesis and potential applications in medicinal chemistry.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Kalyani Jangam, Yu-Yen Chen, Lang Qin, Liang-Shih Fan
Summary: This study demonstrates a novel method for H2S decomposition and clean fuel H-2 production using iron-based sulfur carriers, showcasing an innovative sulfur looping scheme for enhanced H2S conversion. Mo doping accelerates surface hydrogen diffusion on the sulfur carrier, leading to improved overall H2S conversion rates.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Kerstin Rastaedter, David J. Wurm, Oliver Spadiut, Julian Quehenberger
Summary: This study analyzed and compared the influence of two different types of spargers on Pg/Vl and kla in a 2 L chemostat cultivation of S. acidocaldarius at 75 degrees C and a pH value of 3.0. The scale-up criterion kLa was then used to scale up to 20 L and 200 L. By maintaining a constant kla value, comparable dry cell weight, specific growth rate, specific substrate uptake rates, and viability were observed between all investigated scales. This procedure holds the potential for further scaling up to industrial-sized bioreactors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Leon P. Jenner, Julia M. Kurth, Sebastian van Helmont, Katarzyna P. Sokol, Erwin Reisner, Christiane Dahl, Justin M. Bradley, Julea N. Butt, Myles R. Cheesman
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Microbiology
Maria Loeffler, Julia Feldhues, Sofia S. Venceslau, Lydia Kammler, Fabian Grein, Ines A. C. Pereira, Christiane Dahl
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Corvin Ernst, Kanwal Kayastha, Tobias Koch, Sofia S. Venceslau, Ines A. C. Pereira, Ulrike Demmer, Ulrich Ermler, Christiane Dahl
Summary: Many bacteria and archaea utilize a novel pathway of sulfur oxidation similar to that of methanogens, involving a key role of heterodisulfide reductase (Hdr) in the reaction. The structural analysis of Hdr-like proteins from sulfur oxidizers revealed similarities and differences with methanogenic Hdr proteins in terms of electron transfer and reaction mechanisms. The findings suggest a potential mechanism linking protein-bound sulfane oxidation to sulfite in sulfur oxidizers.
Article
Microbiology
Maria Loeffler, Kai B. Wallerang, Sofia S. Venceslau, Ines A. C. Pereira, Christiane Dahl
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Lena Appel, Max Willistein, Christiane Dahl, Ulrich Ermler, Matthias Boll
Summary: In methanogenic archaea, the HdrABC and MvhAGD complexes couple the reduction of CO2 and heterodisulfide via flavin-based electron bifurcation. The modular architecture of HdrA(BC) enzymes allows for functional diversity, but questions regarding electron transfer and energy coupling processes remain. Despite known crystal structures, fundamental aspects of HdrA(BC) core modules integrated into electron-transfer machineries are still being explored.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Microbiology
Aurelie Hirschler, Christine Carapito, Loic Maurer, Julie Zumsteg, Claire Villette, Dimitri Heintz, Christiane Dahl, Ashraf Al-Nayal, Vartul Sangal, Huda Mahmoud, Alain Van Dorsselaer, Wael Ismail
Summary: The study investigated sulfur metabolism in fuel-biodesulfurizing bacterium Rhodococcus qingshengii IGTS8 under different sulfur sources, revealing the complex physiological adaptations and metabolic reprogramming. The biodesulfurizing bacterium exhibited diverse responses to sulfur sources, including limiting sulfur assimilation, activating alternative sulfur assimilation pathways, and adjusting biosynthesis of sulfur-containing metabolites. The findings emphasize the importance of sulfur metabolism in shaping the biodesulfurization phenotype and provide potential targets for enhancing biodesulfurization catalytic activity through metabolic engineering.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Tomohisa Sebastian Tanabe, Christiane Dahl
Summary: This study develops a HMM-supported tool for analyzing protein sequences related to sulphur compound metabolism and validates its performance. By analyzing genomes from environmental samples, the metabolic capacity of microbial communities can be revealed.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Multidisciplinary Sciences
Joo-Han Gwak, Samuel Imisi Awala, Ngoc-Loi Nguyen, Woon-Jong Yu, Hae-Young Yang, Martin von Bergen, Nico Jehmlich, K. Dimitri Kits, Alexander Loy, Peter F. Dunfield, Christiane Dahl, Jung-Ho Hyun, Sung-Keun Rhee
Summary: This study reports the isolation of a new aerobic methanotrophic bacterium capable of oxidizing both methane and reduced sulfur compounds for growth. Genomic and proteomic analyses revealed its metabolic pathways for methane and sulfur oxidation. The discovery of this versatile bacterium highlights the intimate interactions of methane and sulfur cycles in the oxic-anoxic interface environments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Christiane Dahl, Tomohisa S. Tanabe, Jingjing Li, Martina Grosser, Carolin Kuempel
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemistry & Molecular Biology
Jingjing Li, Julian Koch, Wanda Flegler, Leon Garcia Ruiz, Natalie Hager, Alina Ballas, Tomohisa S. Tanabe, Christiane Dahl
Summary: In Hyphomicrobium denitrificans XT, there are two different pathways for thiosulfate oxidation. The simultaneous presence of methanol and thiosulfate negatively affects the growth rate. The formation of sulfite during thiosulfate oxidation may specifically inhibit methanol degradation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Chemistry, Multidisciplinary
Leon P. Jenner, Jason C. Crack, Julia M. Kurth, Zuzana Soldanova, Linda Brandt, Katarzyna P. Sokol, Erwin Reisner, Justin M. Bradley, Christiane Dahl, Myles R. Cheesman, Julea N. Butt
Summary: This study describes the mechanism of Campylobacter jejuni thiosulfate dehydrogenase in the reaction with sulfite, and proposes a catalytic mechanism for thiosulfate oxidation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biotechnology & Applied Microbiology
Julie Zumsteg, Aurelie Hirschler, Christine Carapito, Loic Maurer, Claire Villette, Dimitri Heintz, Christiane Dahl, Ashraf El Nayal, Vartul Sangal, Huda Mahmoud, Alain Van Dorsselaer, Wael Ismail
Summary: Comparative proteomics and untargeted metabolomics were used to study the physiological and metabolic adaptations of Rhodococcus qingshengii IGTS8 under biodesulfurization conditions. The study identified differentially produced proteins and metabolites associated with various metabolic and physiological processes. Increased production of redox cofactors and associated proteins was one of the most significant adaptations.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tomohisa Sebastian Tanabe, Christiane Dahl
Summary: The global sulphur cycle has important implications for various aspects including human health, climate change, biogeochemistry, and bioremediation. Organic sulphur compounds play a vital role in this cycle as a storage reservoir of sulphur and a source of energy and/or carbon for prokaryotes. However, the poor conservation of relevant pathways and their variation within strains of the same species have hindered ecological and evolutionary studies of organic sulphur compound conversion. To overcome this challenge, HMSS2, a profiled Hidden Markov Model-based tool, has been developed for rapid annotation and synteny analysis of organic and inorganic sulphur cycle proteins in prokaryotic genomes.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Biochemistry & Molecular Biology
Tomohisa Sebastian Tanabe, Martina Grosser, Lea Hahn, Carolin Kuempel, Hanna Hartenfels, Evelyn Vtulkin, Wanda Flegler, Christiane Dahl
Summary: This paper investigates a novel pathway for lipoate assembly in cells, revealing new enzymes and coenzymes involved. Through extensive homology and genomic context analyses, it uncovers a wider distribution of lipoate biogenesis systems than expected and provides an evolutionary framework for these systems.
Article
Microbiology
Carolin Kuempel, Fabian Grein, Christiane Dahl
Summary: When oxidizing reduced sulfur compounds, the phototrophic sulfur bacterium Allochromatium vinosum forms sulfur globules as obligatory intracellular intermediates. These globules consist of three hydrophobic proteins: SgpA, SgpB, and SgpC. In addition, a fourth protein SgpD was identified in purified sulfur globules. The in vivo function of SgpD was investigated by fusing its carboxy-terminus to mCherry, and the results showed that SgpD targets mCherry to the periplasm and co-localizes with sulfur deposits in A. vinosum cells.