Article
Cell Biology
Stefan Gaussmann, Mohanraj Gopalswamy, Maike Eberhardt, Maren Reuter, Peijian Zou, Wolfgang Schliebs, Ralf Erdmann, Michael Sattler
Summary: In this study, the membrane interactions of PEX5 and PEX14 NTDs were characterized in vitro using membrane mimicking bicelles and nanodiscs, revealing weak interaction of PEX14 NTD with bicelles and multiple interaction sites of PEX5 NTD involving amphipathic alpha-helical regions. Interestingly, the interaction between PEX5 and PEX14 NTDs was found to be largely unaffected by the presence of the membrane, suggesting that docking of PEX5 to PEX14 at the membrane does not reduce the overall binding affinity between the two proteins in the assembly of the peroxisome translocon.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Yousef Al-Hajaya, Barbara Karpinska, Christine H. Foyer, Alison Baker
Summary: This study reveals conflicting conclusions about the peroxisome targeting signal of catalase and the possibility of catalase being hijacked to the nucleus by effector proteins of plant pathogens. It also highlights the interference of C-terminal tagging and alterations with the nuclear localization of catalase.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Cell Biology
Xiaoman You, Shanshan Zhu, Haowen Sheng, Zheng Liu, Dan Wang, Min Wang, Xiao Xu, Feng He, Hong Fang, Fan Zhang, Debao Wang, Zeyun Hao, Ruyi Wang, Yinghui Xiao, Jianmin Wan, Guo-Liang Wang, Yuese Ning
Summary: Knockdown of OsPEX5 enhances resistance to rice blast fungus Magnaporthe oryzae. OsPEX5 interacts with E3 ubiquitin ligase APIP6 to regulate plant immunity. Additionally, OsPEX5 interacts with OsALDH2B1 to enhance its repression of defense gene expression.
Article
Multidisciplinary Sciences
Valeria Napolitano, Charlotte A. Softley, Artur Blat, Vishal C. Kalel, Kenji Schorpp, Till Siebenmorgen, Kamyar Hadian, Ralf Erdmann, Michael Sattler, Grzegorz M. Popowicz, Grzegorz Dubin
Summary: Trypanosomiasis is a life-threatening infection and requires new therapeutic approaches. In this study, the researchers identified the PEX5-PTS1 interaction as a key player in the import of glycolytic enzymes in Trypanosoma. They developed a fluorescence polarization-based method and successfully identified small molecule inhibitors that can disrupt this interaction and inhibit parasite growth in cell culture.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Maren Reuter, Hamed Kooshapur, Jeff-Gordian Suda, Stefan Gaussmann, Alexander Neuhaus, Lena Bruehl, Pratima Bharti, Martin Jung, Wolfgang Schliebs, Michael Sattler, Ralf Erdmann
Summary: Human PEX14 acts as a dual role in peroxisomal protein import and microtubule anchoring, with its N-terminal domain binding both protein ligands and microtubular filaments. PEX14 interacts with beta-tubulin in spatial proximity to kinesin-binding site on microtubules, and its binding to tubulin can be blocked by PEX5, suggesting a competitive correlation between peroxisome anchoring and motility for homogeneous dispersion in mammalian cells.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eden Yifrach, Markus Rudowitz, Luis Daniel Cruz-Zaragoza, Asa Tirosh, Zohar Gazi, Yoav Peleg, Markus Kunze, Miriam Eisenstein, Wolfgang Schliebs, Maya Schuldiner, Ralf Erdmann, Einat Zalckvar
Summary: This study reveals that the targeting specificity of peroxisomal proteins in yeast is mainly determined by the hydrophobic nature of the amino acid preceding the PTS1 signal. Structural modeling shows differences in surface hydrophobicity of the PTS1-binding cavities of the two factors. This work elucidates the mechanism by which targeting specificity is achieved, enabling dynamic rewiring of the peroxisomal proteome in response to changing metabolic needs.
BIOLOGICAL CHEMISTRY
(2023)
Article
Genetics & Heredity
Muhammad Ali, Shahid Y. Khan, Tony A. Rodrigues, Tania Francisco, Xiaodong Jiao, Hang Qi, Firoz Kabir, Bushra Irum, Bushra Rauf, Asma A. Khan, Azra Mehmood, Muhammad Asif Naeem, Muhammad Zaman Assir, Muhammad Hassaan Ali, Mohsin Shahzad, Khaled K. Abu-Amero, Shehla Javed Akram, Javed Akram, Sheikh Riazuddin, Saima Riazuddin, Michael L. Robinson, Myriam Baes, Jorge E. Azevedo, J. Fielding Hejtmancik, S. Amer Riazuddin
Summary: A novel missense mutation in PEX5 was identified in a large family, resulting in defective import of PTS2 cargo proteins into peroxisomes, leading to congenital cataracts and developmental defects.
Article
Cell Biology
Julia Ast, Nils Baecker, Elena Bittner, Domenica Martorana, Humda Ahmad, Michael Boelker, Johannes Freitag
Summary: Peroxisomes play a crucial role in metabolism, but the mechanism of protein import mediated by Pex5 family proteins and their contribution to organelle function remains unclear. This study analyzed variants of two Pex5 proteins from the fungus Ustilago maydis and found that they have different roles in importing specific matrix proteins. These findings enhance our understanding of peroxisomal protein import in other biological systems.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael L. Skowyra, Tom A. Rapoport
Summary: Peroxisomes are important organelles that can cause severe diseases when their function is impaired. This study provides insights into the mechanism by which PEX5 transports cargo into peroxisomes and undergoes recycling.
Article
Biochemistry & Molecular Biology
Serhii Chornyi, Claudio F. Costa, Lodewijk IJlst, Marc Fransen, Ronald J. A. Wanders, Carlo W. T. van Roermund, Hans R. Waterham
Summary: This study developed a method to measure intraperoxisomal NADH levels in human cells and found that the NAD+/NADH ratio in cytosol and peroxisomes are closely connected, mediated by intraperoxisomal lactate and malate dehydrogenases. This study provides evidence for the existence of two independent redox shuttle systems in human peroxisomes that regulate peroxisomal NAD+/NADH homeostasis. It also demonstrates the specific metabolic function of protein isoforms generated by translational stop codon readthrough in humans.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Cell Biology
Amr Kataya, Nitija Gautam, Muhammad Jamshed, Douglas G. Muench, Marcus A. Samuel, Jay J. Thelen, Greg B. Moorhead
Summary: This study identified protein kinases in peroxisomes and investigated their functions and regulation. The findings provide new insights into the regulation of peroxisome functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joyce Bagyi, Veda Sripada, Andrea M. Aidone, H. -Y. Lin, Elizabeth H. Ruder, Dana R. Crawford
Summary: Nutrigenomics is the study of how food and nutrients impact gene expression, aiming to design precision nutrition strategies for human health. Modulation of redox-associated genes through healthy diet can treat diseases, reduce treatment costs, and improve health outcomes, with potential clinical significance.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jean-Claude Farre, Paul Li, Suresh Subramani
Summary: This study investigates the localization of NADH-shuttling proteins in Pichia pastoris and introduces a novel BiFC variant called divergent BiFC for detecting colocalization of noninteracting proteins. The findings demonstrate a partial peroxisomal location of a specific NADH-shuttling protein only under certain growth conditions, shedding light on the medium-dependent compartmentalization of proteins in the yeast.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Anna Grochmal, Ben Woods, Lilia Milanesi, Manuel Perez-Soto, Salvador Tomas
Summary: Research on mimicking biological systems reveals that minimal environmental changes can trigger high avidity membrane receptor assembly, which can be modulated by weakly binding divalent ligands. Mathematical models and equations can predict the modulation intensity of the ligand-messenger on the ligand-receptor ON signal, providing valuable tools for studying membrane receptors in biological and biomimetic systems.
Article
Chemistry, Multidisciplinary
Yi Du, Qiwei Wang, Lili Shi, Tao Li
Summary: DNA-assembled multiaptamer systems offer a promising approach for targeted delivery of nucleic acid cargoes. In this study, a G-quadruplex-proximized aptamer system was engineered for efficient transmembrane delivery of siRNA, showing great potential for precise anticancer therapy.
Article
Toxicology
Danielle Hagstrom, Lisa Truong, Siqi Zhang, Robert Tanguay, Eva-Maria S. Collins
TOXICOLOGICAL SCIENCES
(2019)
Article
Plant Sciences
Peng-Cheng Li, Ke Li, Juan Wang, Chuan-Zhi Zhao, Shu-Zhen Zhao, Lei Hou, Han Xia, Chang-Le Ma, Xing-Jun Wang
Article
Cell Biology
Xiaofeng Wang, Pingping Wang, Zhuangzhuang Zhang, Jean-Claude Farre, Xuezhi Li, Ruonan Wang, Zhijie Xia, Suresh Subramani, Changle Ma
Editorial Material
Plant Sciences
Yuebin Jia, Huiyu Tian, Shuo Zhang, Zhaojun Ding, Changle Ma
TRENDS IN PLANT SCIENCE
(2019)
Review
Biochemistry & Molecular Biology
Kai Hua, Jinshan Zhang, Jose Ramon Botella, Changle Ma, Fanjiang Kong, Baohui Liu, Jian-Kang Zhu
Review
Plant Sciences
Tong Su, Wenjing Li, Pingping Wang, Changle Ma
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Environmental Sciences
Danielle Ireland, Veronica Bochenek, Daniel Chaiken, Christina Rabeler, Sumi Onoe, Ameet Soni, Eva-Maria S. Collins
Article
Plant Sciences
Yuebin Jia, Xiangpei Kong, Kongqin Hu, Mengqiang Cao, Jiajia Liu, Changle Ma, Siyi Guo, Xianzheng Yuan, Shan Zhao, Helene S. Robert, Cuiling Li, Huiyu Tian, Zhaojun Ding
Article
Multidisciplinary Sciences
Ziad Sabry, Christina Rabeler, Danielle Ireland, Kevin Bayingana, Eva-Maria S. Collins
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Genetics & Heredity
Fengdan Guo, Lei Hou, Changle Ma, Guanghui Li, Ruxia Lin, Yanxiu Zhao, Xingjun Wang
Summary: This study generated a peanut mutant sdm1 through fast neutron irradiation, and identified significant differences in the structure and hormone pathways between sdm1 and the conventional variety SH13. This finding is crucial for uncovering the molecular mechanism of peanut plant height regulation.
Article
Biochemistry & Molecular Biology
Xiaorui Huang, Fei Su, Sheng Huang, Fating Mei, Xiaomu Niu, Changle Ma, Hui Zhang, Xiaoguo Zhu, Jian-Kang Zhu, Jinshan Zhang
Summary: Novel alleles of the Waxy gene, including the waxy(abe2) allele, were generated by nucleotide substitutions in the middle region of the Wx protein, leading to improvements in amylose content and cooking quality of rice grains. The waxy(abe2) allele exhibited desirable characteristics without undesirable agronomic traits, providing a promising breeding material for enhancing rice grain quality.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Correction
Plant Sciences
Yuebin Jia, Xiangpei Kong, Kongqin Hu, Mengqiang Cao, Jiajia Liu, Changle Ma, Siyi Guo, Xianzheng Yuan, Shan Zhao, Helene S. Robert, Cuiling Li, Huiyu Tian, Zhaojun Ding
Article
Biotechnology & Applied Microbiology
Ping Lv, Fei Su, Fangyuan Chen, Chunxue Yan, Dandan Xia, Hui Sun, Shanshan Li, Zhiqiang Duan, Changle Ma, Hui Zhang, Mugui Wang, Xiaomu Niu, Jian-Kang Zhu, Jinshan Zhang
Summary: This study characterized the genome editing application of Cas12i3, a type V-I family endonuclease, in rice. A CRISPR/Cas12i3-based iMAGE system was developed, which efficiently edited various genes in rice and produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. Base editors using deactivated Cas12i3 were also developed, resulting in the generation of herbicide-resistant rice plants. These CRISPR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Chenyang Li, Qiuyue Hu, Zhen Luo, Xiaowen Wang, Wei Tang, Hequan Lu, Changle Ma, Xiangqiang Kong
Summary: This study reveals that nonuniform root salinity distribution increases nitrate uptake in cotton by transmitting GhCEP and GhCEPD signals between roots and shoots.
Article
Chemistry, Multidisciplinary
Yongqing Zhou, Ping Li, Nannan Fan, Xin Wang, Xiaoning Liu, Lijie Wu, Wen Zhang, Wei Zhang, Changle Ma, Bo Tang
CHEMICAL COMMUNICATIONS
(2019)