Article
Biochemistry & Molecular Biology
Xuan Xiong, Xiaoqin Zhang, Yuan Zhang, Jiaqi Xie, Yuan Bian, Qinan Yin, Rongsheng Tong, Dongke Yu, Lingai Pan
Summary: This study found that TCEP exposure can cause cardiac fibrosis by inhibiting the expression of SERCA and leading to Ca2+ overload, which in turn triggers ER stress and overactive autophagy. The results reveal the important role of the Ca2+ overload/ER stress/autophagy axis in TCEP-induced cardiotoxicity.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Engineering, Environmental
Jing Zheng, Shane Antrobus, Wei Feng, Trevor N. Purdy, Bradley S. Moore, Isaac N. Pessah
Summary: This study found that bromopyrroles can influence neuronal network activity, with higher concentrations of TBP leading to a slow and sustained elevation in cytoplasmic calcium levels, ultimately resulting in loss of neuronal viability. The potential ecological and health impacts of bromopyrroles warrant further investigation.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Physiology
Lianguo Wang, Rachel C. Myles, I-Ju Lee, Donald M. Bers, Crystal M. Ripplinger
Summary: The study demonstrates that inhibition of SERCA can slow down SR Ca2+ reuptake, leading to an increase in the magnitude of Ca2+ alternans in cardiac cells, especially at fast pacing frequencies. Notably, the alternation of SR Ca2+ release precedes the alternation of SR Ca2+ load during rapid ventricular pacing.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Nishadh Rathod, Jessi J. Bak, Joseph O. Primeau, M'Lynn E. Fisher, Lennane Michel Espinoza-Fonseca, Mary Joanne Lemieux, Howard S. Young
Summary: SERCA is responsible for maintaining calcium homeostasis in cells and is regulated by the regulin family, including PLN and SLN, which inhibit its calcium transport properties. New members of the regulin family, such as MLN, ELN, and ALN, have been discovered and shown to have distinct inhibitory effects on SERCA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Ha Thu Nguyen, Carlos Noriega Polo, Andreas Wiederkehr, Claes B. Wollheim, Kyu-Sang Park
Summary: Enhancing endoplasmic reticulum (ER) calcium uptake through activation of the sarco/endoplasmic reticulum calcium ATPase (SERCA) can improve survival and function of pancreatic ss-cells. Activation of SERCA increases insulin synthesis and exocytosis, enhances sensitivity to glucose, and improves mitochondrial bioenergetics and antioxidant capability. Targeting SERCA could be a promising therapeutic strategy for protecting ss-cells from lipotoxicity and Type 2 diabetes development.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Cell Biology
Toyokazu Kimura, Kazuki Kagami, Atsushi Sato, Ayumu Osaki, Kei Ito, Shunpei Horii, Takumi Toya, Nobuyuki Masaki, Risako Yasuda, Yuji Nagatomo, Takeshi Adachi
Summary: This study found that SERCA2 protein expression decreased in the aorta of diabetic mice, and CDN1163 improved aortic endothelial dysfunction, glucose tolerance, hepatosteatosis, and skeletal muscle function in these mice.
Article
Oncology
Zhidong Li, Jia Guo, Yunfei Bian, Mingsheng Zhang
Summary: The study found that IMD can protect cardiomyocytes against thapsigargin-induced apoptosis, at least partially by activating the protein kinase A/SERCA pathway.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Weixing Zhu, Yanwei Duan, Jiqiang Chen, Hans Merzendorfer, Xu Zou, Qing Yang
Summary: The study identified a novel role of DmSERCA in chitin synthesis, demonstrating its physical interaction with epidermal chitin synthase and its importance in chitin deposition and structure formation in Drosophila melanogaster.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Iyuki Namekata, Ryosuke Odaka, Shunsuke Hamazaki, Hina Nisaka, Shogo Hamaguchi, Hikaru Tanaka
Summary: A method using fluorescence microscopy and specific inhibitors was developed to evaluate the activity of NCX and SERCA in mouse ventricular cardiomyocytes. Different adrenoceptor stimulations and inhibitors exhibited distinct effects on cytoplasmic Ca2+ concentration in non-beating ventricular cardiomyocytes, suggesting potential applications for pharmacological evaluations targeting NCX and SERCA.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Pharmacology & Pharmacy
Silvia Romero-Sanz, Elena Caldero-Escudero, Pilar Alvarez-Illera, Jaime Santo-Domingo, Rosalba I. I. Fonteriz, Mayte Montero, Javier Alvarez
Summary: The high prevalence of neurodegenerative diseases in the population and the lack of effective treatments have prompted the search for new therapeutic targets. Submaximal inhibition of SERCA, the main protein responsible for ER calcium storage, has been found to increase lifespan in Caenorhabditis elegans worms. In this study, the effects of submaximal SERCA inhibition in a chemical model of Parkinson's disease induced in C. elegans worms were investigated.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Anatomy & Morphology
Wanying Chen, Mengmeng Li, Xiangyu Lei, Yawen Cheng, Xiaoyu Wu, Xinyue Sun, Yixuan Wu, Zhiheng Li, Guogang Luo, Meng Wei
Summary: Our study found that remote ischemic postconditioning (RIPostC) can alleviate brain ischemic injury by reducing endoplasmic reticulum (ER) stress, providing a theoretical basis for the protective mechanism of ischemic stroke.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Biochemistry & Molecular Biology
Ayako Takeuchi, Satoshi Matsuoka
Summary: In this study, the spatial and functional coupling between mitochondrial Na+-Ca2+ exchanger (NCLX) and sarcoplasmic reticulum Ca2+ pump SERCA in cardiomyocytes was investigated. The results demonstrated a close interaction between NCLX and SERCA, indicating their important role in modulating cellular functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Junjie Yu, Wenrong Feng, Xue Chen, Changyou Song, Shengyan Su, Jiachun Ge, Yongkai Tang
Summary: This study cloned a cDNA encoding a putative SERCA protein from the Y-organs of E. sinensis. The Es-SERCA protein was widely expressed in various tissues, with the highest expression in the muscle. The study also revealed the functional role of SERCA in calcium homeostasis and its involvement in ecdysteroid synthesis during the moulting cycle.
AQUACULTURE RESEARCH
(2022)
Article
Cell Biology
Elisa Bovo, Roman Nikolaienko, Daniel Kahn, Ellen Cho, L. Seth Robia, V. Aleksey Zima
Summary: PS1 serves as an important regulator of cardiac Ca2+ pump function by interacting with SERCA2a, enhancing Ca2+ uptake at low ER Ca2+ loads and reducing uptake at high loads.
Article
Cardiac & Cardiovascular Systems
Jill K. Badin, Caleb Eggenberger, Stacey Dineen Rodenbeck, Zubair A. Hashmi, I-Wen Wang, Jose P. Garcia, Mouhamad Alloosh, Michael Sturek
Summary: In coronary smooth muscle of atherosclerotic coronary artery disease in metabolic syndrome swine, intracellular free Ca2+ dysregulation occurs. Increased [Ca2+](i) signaling in human CAD is associated with advanced age and a greater media area, while decreased signaling is related to a higher number of risk factors and plaque burden in human and swine CAD. Similar [Ca2+](i) dysregulation in human and Ossabaw swine CSM supports the translational relevance of this large animal model.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Furkan E. Oflaz, Zhanat Koshenov, Martin Hirtl, Rene Rost, Roland Malli, Wolfgang F. Graier
Summary: The study revealed that S1R plays an important role in cancer energy metabolism. Activation of S1R increases mitochondrial bioenergetics while reducing reliance on aerobic glycolysis in cancer cells. S1R antagonist, on the other hand, increases aerobic glycolysis in S1R expressing cancer cell line.
Article
Biochemistry & Molecular Biology
Christiane Klec, Erik Knutsen, Daniela Schwarzenbacher, Katharina Jonas, Barbara Pasculli, Ellen Heitzer, Beate Rinner, Katarina Krajina, Felix Prinz, Benjamin Gottschalk, Peter Ulz, Alexander Deutsch, Andreas Prokesch, Stephan W. Jahn, S. Mohammad Lellahi, Maria Perander, Raffaela Barbano, Wolfgang F. Graier, Paola Parrella, George Adrian Calin, Martin Pichler
Summary: The RNA-binding protein ALYREF plays a significant role in breast carcinogenesis by regulating the two isoforms of NEAT1, which in turn affects cellular growth, apoptosis, and mitochondrial energy metabolism. This study provides novel insights into the molecular mechanisms underlying breast cancer and has important implications for its treatment and prevention.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Emrah Eroglu, Wolfgang F. Graier, Roland Malli
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Transplantation
Andras T. Deak, Katarina Belic, Anna-Maria Meissl, Katharina Artinger, Kathrin Eller, Bernd Rechberger, Tobias Niedrist, Wolfgang F. Graier, Roland Malli, Helmut Bischof, Sandra Burgstaller, Sandra Blass, Alexander Avian, Alexander R. Rosenkranz, Alexander H. Kirsch
Summary: This study assessed the performance of genetically encoded potassium-ion indicators (GEPIIs) in measuring salivary potassium concentration and found that salivary potassium levels exhibited similar kinetics to plasma potassium levels. However, further research is needed to determine if saliva could be a suitable sample type for monitoring plasma potassium levels.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Article
Biology
Benjamin Gottschalk, Zhanat Koshenov, Markus Waldeck-Weiermair, Snjezana Radulovic, Furkan E. Oflaz, Martin Hirtl, Olaf A. Bachkoenig, Gerd Leitinger, Roland Malli, Wolfgang F. Graier
Summary: This study reveals the role of MICU1 in controlling the spatial membrane potential gradients in mitochondria, with changes in SMPGs and spatial cristae depolarization occurring when Ca2+ binds to MICU1.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Furkan E. Oflaz, Zhanat Koshenov, Martin Hirtl, Olaf A. Bachkoenig, Wolfgang F. Graier, Benjamin Gottschalk
Summary: Mitochondrial uncoupling proteins UCP1 and UCP2 have different molecular mechanisms for executing their mitochondria uncoupling function, with UCP1 dissipating the proton motive force and UCP2 modulating the permeability of the cristae junction. This mini-review discusses the molecular mechanisms of UCP1 in brown adipose tissue and UCP2 in aged and cancer non-excitable cells, and the synergistic effects of both UCPs with the mitochondrial Ca2+ uptake machinery.
Article
Genetics & Heredity
Lukas Kaufmann, Johannes Pilic, Lisa Auinger, Anna-Lena Mayer, Jasmin Blatterer, Johann Semmler-Bruckner, Safdar Abbas, Khurram Rehman, Muhammad Ayaz, Wolfgang F. Graier, Roland Malli, Erwin Petek, Klaus Wagner, Ali Al Kaissi, Muzammil Ahmad Khan, Christian Windpassinger
Summary: Restrictive dermopathy (RD) is a lethal condition caused by loss-of-function mutations in ZMPSTE24, while mutations preserving residual enzymatic activity lead to mandibuloacral dysplasia with type B lipodystrophy (MADB) phenotype. A new homozygous loss-of-function mutation in ZMPSTE24 was identified in two consanguineous Pakistani families with MADB. Functional analysis revealed that alternative translation initiation sites were utilized, preventing complete loss of protein function and explaining the relatively mild phenotype in affected patients. Our findings suggest considering the creation of new potential start codons in other disease-associated genes with N-terminal mutations.
Article
Biochemistry & Molecular Biology
Madeleine Goeritzer, Katharina B. Kuentzel, Sarah Beck, Melanie Korbelius, Silvia Rainer, Ivan Bradic, Dagmar Kolb, Marion Mussbacher, Waltraud C. Schrottmaier, Alice Assinger, Axel Schlagenhauf, Rene Rost, Benjamin Gottschalk, Thomas O. Eichmann, Thomas Zuellig, Wolfgang F. Graier, Nemanja Vujic, Dagmar Kratky
Summary: Monoglyceride lipase (MGL) hydrolyzes monoacylglycerols (MG) and plays a role in platelet function. MGL deficiency leads to decreased platelet aggregation and impaired response to collagen activation, resulting in reduced thrombus formation and prolonged bleeding time. Genetic deletion of MGL is associated with altered thrombogenesis in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Mathematics, Interdisciplinary Applications
Claire Jean-Quartier, Katharina Bein, Lukas Hejny, Edith Hofer, Andreas Holzinger, Fleur Jeanquartier
Summary: In response to socioeconomic development, this study focuses on the transparency and sustainability aspects of artificial intelligence in terms of energy consumption. The research measures carbon emissions and energy consumption of Python algorithms and tests the impact of explainability on algorithmic energy consumption. The results can guide the selection of tools to measure algorithmic energy consumption and raise awareness of emission-based model optimization by highlighting the sustainability of explainable artificial intelligence.
Article
Gastroenterology & Hepatology
Sarah Duponchel, Lea Monnier, Jennifer Molle, Nadia Bendridi, Muhammad Rizwan Alam, Ahmed Gaballah, Boyan Grigorov, Alexander Ivanov, Marcel Schmiel, Margarete Odenthal, Michel Ovize, Jennifer Rieusset, Fabien Zoulim, Birke Bartosch
Summary: Chronic HCV infection causes cellular stress, fibrosis, and predisposes to hepatocarcinogenesis. Mitochondria play key roles in orchestrating stress responses. The study revealed that HCV proteins associate with mitochondria and alter the composition of mitochondrial subdomains, which are crucial for efficient viral replication.
Editorial Material
Cell Biology
Benjamin Gottschalk, Roland Malli, Wolfgang F. Graier
Summary: Changes in mitochondrial ultrastructure and dynamics, including cytochrome c release, membrane potential rearrangement, and mitochondrial Ca2+ uptake dynamics, are observed in MICU1(-/-) cells due to its sensitivity to Ca2+ and its binding to Mic60 and CHCHD2. MICU1 is not only an interaction partner and regulator of the MCU complex, but also plays a crucial role in determining mitochondrial ultrastructure and initiating apoptosis.
Article
Biochemistry & Molecular Biology
Madiha Amin Malik, Muhammad Arif Nadeem Saqib, Edwin Mientjes, Anushree Acharya, Muhammad Rizwan Alam, Ilse Wallaard, Isabelle Schrauwen, Michael J. Bamshad, Regie Lyn P. Santos-Cortez, Ype Elgersma, Suzanne M. Leal, Muhammad Ansar
Summary: Intellectual disability (ID) and retinal dystrophy (RD) are common features of multiple syndromes. A study on a family with severe ID and retinitis pigmentosa (RP) identified a genetic variant in the AGPAT3 gene, suggesting its role in the development of ID and RP syndrome.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Chemistry, Multidisciplinary
Zuzana Klepcova, Ivana Spakova, Corina T. T. Madreiter-Sokolowski, Wolfgang Graier, Katarina Kalinova, Erika Samolova, Romana Smolkova, Lukas Smolko, Miroslava Rabajdova
Summary: Two novel Mn(II) complexes with fenamic acid and flufenamic acid as ligands and neocuproine as a supporting ligand were synthesized and characterized. The complexes exhibit moderate radical scavenging activity and cytotoxic effects on selected cell lines, with the flufenamic acid complex being able to induce apoptosis. Both complexes target inflammatory processes on a cellular level.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Mehvish Batool, Hajra Fayyaz, Muhammad Rizwan Alam
Summary: This study reveals asymmetric mPTP opening in mouse brain hemispheres, which is not associated with the mRNA levels of MCUC components. Additionally, kidney mitochondria show larger and faster mPTP opening compared to brain mitochondria.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Computer Science, Information Systems
Claire Jean-Quartier, Miguel Rey Mazon, Mario Lovric, Sarah Stryeck
Summary: Research and development benefit from sharing knowledge bases and collaborative data utilization. However, most companies and organizations store their data in data silos, which hinders knowledge creation. To enable collaborative data sharing, public and private actors need to adopt a flexible approach and break data silos. This paper investigates the factors influencing cooperative data usage and explores the challenges of participating in cross-organizational data ecosystems, highlighting the technological and organizational requirements for collaborative data usage through an interview study with stakeholders from private and public organizations in the IDE@S project in Styria, Austria.