Article
Cell Biology
Amr Kataya, Nitija Gautam, Muhammad Jamshed, Douglas G. Muench, Marcus A. Samuel, Jay J. Thelen, Greg B. Moorhead
Summary: This study identified protein kinases in peroxisomes and investigated their functions and regulation. The findings provide new insights into the regulation of peroxisome functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Reyhane Masoudifar, Niki Pouyanfar, Dongfei Liu, Mahnaz Ahmadi, Behnaz Landi, Mahsa Akbari, Sina Moayeri-Jolandan, Fatemeh Ghorbani-Bidkorpeh, Elham Asadian, Mohammad-Ali Shahbazi
Summary: The precision encapsulation ability of metal-organic frameworks (MOFs) makes them efficient nanocarriers for various pharmaceuticals. After characterization and surface modification, MOFs exhibit high internal surface area and active targeting delivery capability, making them promising for disease treatment. This review provides an overview of MOF structures, synthesis approaches, functionalization strategies, and future applications.
APPLIED MATERIALS TODAY
(2022)
Article
Biochemistry & Molecular Biology
Marco Anteghini, Asmaa Haja, Vitor A. P. Martins dos Santos, Lambert Schomaker, Edoardo Saccenti
Summary: We present the OrganelX e-Science Web Server that provides a user-friendly implementation of sub-peroxisomal and sub-mitochondrial localization classifiers for peroxisomal and mitochondrial proteins and an algorithm for detecting and validating potential peroxisomal proteins carrying a PTS1 signal sequence. The server is accessible at https://organelx.hpc.rug.nl/fasta/.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Immunology
Fan Yang, Nathachit Limjunyawong, Qi Peng, John T. Schroeder, Sarbjit Saini, Donald MacGlashan Jr, Xinzhong Dong, Li Gao
Summary: In this study, the researchers aimed to identify MRGPRX2-selective and potent agonists as drug candidates responsible for clinical features of pseudo-allergic drug hypersensitivity reactions (DHRs). Through screening and validation, they discovered several active MRGPRX2 agonists and classified them into different drug classes. These findings are important for personalized prevention and treatment of DHRs, as well as pharmacogenetic investigations of MRGPRX2 in relevant disease cohorts.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Diego Piacentini, Federica Della Rovere, Ilaria Bertoldi, Lorenzo Massimi, Adriano Sofo, Maria Maddalena Altamura, Giuseppina Falasca
Summary: This research investigated the role of PEX7 in Arabidopsis root system exposed to Cd stress. The mutant showed reduced Cd translocation to shoots and altered peroxisomal metabolism. The mutation also affected ROS metabolism and auxin levels, highlighting the importance of peroxisomes in plant responses to Cd stress.
Review
Biochemistry & Molecular Biology
Morgana K. Kellogg, Sarah C. Miller, Elena B. Tikhonova, Andrey L. Karamyshev
Summary: Signal recognition particle (SRP) is an RNA and protein complex found across all domains of life, with varying compositions and functions in different organisms. Its main function involves targeting proteins to the endoplasmic reticulum and protecting the mRNA of secretory proteins from degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
John Abousawan, Laura A. Orofiamma, Gregory D. Fairn, Costin N. Antonescu
Summary: The epidermal growth factor receptor (EGFR) controls many cellular functions by undergoing dimerization, phosphorylation, and activation of signals including the PI3K-Akt pathway upon ligand binding. The role of flotillin nanodomains in EGFR signaling remains unclear, as some studies suggest their involvement while others show limited effect. This study reveals that HER2 expression alters EGFR signaling duration and requires flotillin-1 and c-Src for Akt activation and cell proliferation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biology
Eleanor Simon, Carlos Jimenez-Jimenez, Irene Seijo-Barandiaran, Gustavo Aguilar, David Sanchez-Hernandez, Adrian Aguirre-Tamaral, Laura Gonzalez-Mendez, Pedro Ripoll, Isabel Guerrero
Summary: The conserved family of Hedgehog (Hh) signaling proteins plays a crucial role in cell-cell communication, inducing concentration-dependent responses in target cells at different distances. The interaction between glypicans and adhesion molecules/Ihog is essential for maintaining Hh signaling and regulating cytoneme dynamics. Ihog, but not Boi, is crucial for the long-range gradient formation in Hh signaling.
Article
Microbiology
Ondrej Cerny, Camilla Godlee, Romina Tocci, Nancy E. Cross, Haoran Shi, James C. Williamson, Eric Alix, Paul J. Lehner, David W. Holden
Summary: The bacterium Salmonella enterica inhibits T cell immune response by affecting T cell activation, mainly through degradation of the CD97 protein to reduce contacts between antigen-presenting cells and T cells. Additionally, Salmonella effector SteD depletes mature MHCII molecules by enhancing ubiquitination of CD97, which affects dendritic cell-T cell interactions independent of its effect on MHCII.
Article
Biochemistry & Molecular Biology
Ya-Yun Chen, Xiang-Hong Ran, Run-Ze Ni, Dan Mu
Summary: TRIM28 is identified as a negative regulator of the RLR signaling pathway by targeting MAVS for proteasome-mediated degradation, which inhibits the production of type I interferons and proinflammatory cytokines induced by MAVS.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Sweata Hanson, Aiswarya Dharan, P. V. Jinsha, Sanjay Pal, Bipin G. Nair, Rekha Kar, Nandita Mishra
Summary: Programmed cell death (PCD) is a universal process that regulates cellular homeostasis and plays a crucial role in disease conditions, including cancer. Apoptosis is a major PCD, but cancer cells develop resistance to it, necessitating the search for alternative forms of programmed cell death such as paraptosis. Paraptosis is characterized by vacuolation and damage to cellular structures and has potential therapeutic implications in cancer treatment.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Health Care Sciences & Services
Mirco Dindo, Giulia Ambrosini, Elisa Oppici, Angel L. Pey, Peter J. O'Toole, Joanne L. Marrison, Ian E. G. Morrison, Elena Butturini, Silvia Grottelli, Claudio Costantini, Barbara Cellini
Summary: Research suggests that peroxisomal matrix proteins are transported into peroxisomes in a fully-folded state, but the import of multimeric proteins as monomers or oligomers is still debated. Monomerization significantly reduces the intracellular stability of AGT and increases its aggregation/degradation tendency, with monomers partly retained in the cytosol.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biology
Akihiro Yamashita, Olesia Ignatenko, Mai Nguyen, Raphaelle Lambert, Kathleen Watt, Caroline Daneault, Isabelle Robillard-Frayne, Ivan Topisirovic, Christine Des Rosiers, Heidi M. McBride
Summary: Peroxisomes play a central role in regulating metabolic and signaling pathways, but the mechanisms by which peroxisome status is communicated and integrated into cellular signaling pathways are not well understood. In this study, the cellular responses to peroxisomal proteotoxic stress were investigated. It was found that depletion of the peroxisomal protease/chaperone LONP2 led to cell-specific stress responses, including the activation of the integrated stress response (ISR) and upregulation of ribosomal biogenesis gene expression levels in COS-7 cells. Common changes between COS-7 and U2OS cell lines included repression of the retinoic acid signaling pathway and upregulation of sphingolipids. Accumulation of cholesterol in endomembrane compartments was observed in both cell lines. These unexpected consequences of peroxisomal stress provide important insights into tissue-specific responses in peroxisomal disorders.
Article
Biochemistry & Molecular Biology
Bushra Saeed, Florian Deligne, Carla Brillada, Kai Duenser, Franck Aniset Ditengou, Ilona Turek, Alaa Allahham, Nenad Grujic, Yasin Dagdas, Thomas Ott, Juergen Kleine-Vehn, Gregory Vert, Marco Trujillo
Summary: Unlike other eukaryotic model organisms, the main sources of K63-linked Ub chains in Arabidopsis are UBC35 and UBC36. Previous research has associated K63-linked chains with vesicle trafficking regulation but lacked definitive evidence for their role in endocytosis. This study demonstrates that K63-Ub chains are essential for endocytic trafficking in plants and are also involved in selective autophagy through the interaction with NBR1. These findings highlight the importance of K63-Ub chains in maintaining proteostasis in plants.
Article
Multidisciplinary Sciences
Wayne D. Harshbarger, Derrick Deming, Gordon J. Lockbaum, Nattapol Attatippaholkun, Maliwan Kamkaew, Shurong Hou, Mohan Somasundaran, Jennifer P. Wang, Robert W. Finberg, Quan Karen Zhu, Celia A. Schiffer, Wayne A. Marasco
Summary: This study presents structural details of the human antibody 3I14 recognizing HA from various influenza A viruses, highlighting the binding mechanism and potential implications for universal vaccine design.
NATURE COMMUNICATIONS
(2021)