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Immunology
Thierry Delaveau, Antonin Thiebaut, Medine Benchouaia, Jawad Merhej, Frederic Devaux
Summary: This study revealed the interactions among CBC, Hap4 and Yap5 in Candida glabrata, showing that Yap5 regulation requires specific promoter sequences, and there is competition between Hap4 and Yap5 at the promoter of iron tolerance genes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Maria Osmala, Gokcen Eraslan, Harri Lahdesmaki
Summary: ChromDMM is a mixture model based on multiple chromatin features, which accurately clusters genomic regions and discovers distinctive patterns of epigenetic signals at regulatory elements. Validated with simulated data and ENCODE data, ChromDMM demonstrates higher accuracy and robustness.
Article
Agronomy
Deshui Yu, Renchun Fan, Ling Zhang, Pengya Xue, Libing Liao, Meizhen Hu, Yanjun Cheng, Jine Li, Ting Qi, Shaojuan Jing, Qiuyun Wang, Arvind Bhatt, Qian-Hua Shen
Summary: Plants have a sophisticated immune system that detects pathogen infection and activates immune responses. HvWRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus. However, the molecular features of HvWRKY2 in its DNA-binding and repressor functions, as well as its target genes, are unknown.
Review
Biochemistry & Molecular Biology
Stefano Amatori, Mirco Fanelli
Summary: Epigenomic aberrations in cancer cells play a role in cancer initiation and progression. Formalin fixation and paraffin embedding (FFPE) is widely used to preserve tissue integrity, and new chromatin immunoprecipitation (ChIP) techniques allow analysis of histone post-translational modifications and transcription factor distribution in FFPE tissues. However, the variability in fixation and storage conditions of clinical specimens can affect the success of ChIP studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Annapurna Bhattacharjee, Prabhakar Lal Srivastava, Onkar Nath, Mukesh Jain
Summary: OsHOX24 mediates regulation of desiccation stress response in rice through binding to multiple target genes involved in various biological processes, such as plant development and abiotic stress responses. The involvement of OsHOX24 in complex regulatory networks is indicated by the enrichment of cis-regulatory motifs representing binding sites of other transcription factors.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Peerapat Khamwachirapithak, David Guillaume-Schoepfer, Pakkanan Chansongkrow, Sarah A. Teichmann, Philip A. Wigge, Varodom Charoensawan
Summary: This study combines ChIP-seq and RNA-seq to investigate the effects of global and local H3 depletion on gene transcription, as well as the interaction between the transcription factor Rap1 and H3. The results provide a working model and testable hypotheses regarding the impact of H3 depletion on transcriptional changes.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Li Zhong, Qing Liu, Qiaofeng Liu, Shikai Zhang, Yongbing Cao, Dehua Yang, Ming-Wei Wang
Summary: The research revealed that W2476 promotes the competitive binding of FOXO1 with the ChoRE-binding protein/Mlx complexes, hindering the acetylation process of the TXNIP promoter, and ultimately reducing TXNIP transcription.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Clinical Neurology
Dulika Sumathipala, Petter Stromme, Zohreh Fattahi, Torben Luders, Ying Sheng, Kimia Kahrizi, Ingunn Holm Einarsen, Jennifer L. Sloan, Hossein Najmabadi, Lambert van den Heuvel, Ron A. Wevers, Sergio Guerrero-Castillo, Lars Morkrid, Vassili Valayannopoulos, Paul Hoff Backe, Charles P. Venditti, Clara D. van Karnebeek, Hilde Nilsen, Eirik Frengen, Doriana Misceo
Summary: Mutations in the ZBTB11 gene are associated with intellectual disability. The study found that patients with ZBTB11 mutations showed atrophy in multiple brain regions and had malonic and methylmalonic aciduria as disease markers. In vitro experiments indicated that ZBTB11 regulates mitochondrial functions and RNA processing.
Review
Biochemistry & Molecular Biology
Niamat Khan, Sidra Shahid, Abdul R. Asif
Summary: Chromatin is a dynamic structure consisting of DNA and proteins, playing key roles in DNA packaging, gene expression regulation, and DNA protection. Studying chromatin-associated proteins can enhance our understanding of disease pathophysiology and aid in designing new drugs.
Article
Biochemistry & Molecular Biology
Yu Chen, Weizhu Zeng, Shiqin Yu, Song Gao, Jingwen Zhou
Summary: This study found that chromatin regulation plays an important role in nitrogen metabolism in Saccharomyces cerevisiae. It identified key nitrogen metabolism genes directly regulated by the chromatin regulator Ahc1p and analyzed the interaction between Ahc1p and transcription factors. The study elucidates the regulatory mechanism of nitrogen metabolism in S. cerevisiae from an epigenetic perspective.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Guoying Zhao, Qian Cheng, Yuting Zhao, Feifei Wu, Bangbang Mu, Jiping Gao, Liu Yang, Jingli Yan, Hanfeng Zhang, Xing Cui, Qinqin Chen, Fangxiao Lu, Qianqian Ao, Asma Amdouni, Yuan-Qing Jiang, Bo Yang
Summary: This study identifies ABA-responsive element binding factors (ABFs) as upstream transcription factors of MAPKKK18 expression, which is involved in abiotic stress priming, drought tolerance, and leaf senescence. ABF2, ABF3, and ABF4 bind to ABA-responsive elements in the promoter of MAPKKK18 and activate its expression. Mutants of mapkkk18 exhibit delayed leaf senescence, while over-expression of ABF2, ABF3, and ABF4 in the background of mapkkk18 phenocopies the stay-green phenotype and reduces the expression of ABF target genes. These findings demonstrate that ABF2, ABF3, and ABF4 act as transcriptional regulators of MAPKKK18 and suggest a role of ABA in priming leaf senescence through ABF-induced expression of MAPKKK18.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Peiyi Zhou, Liqun Ouyang, Ting Jiang, Yingpu Tian, Wenbo Deng, Haibin Wang, Shuangbo Kong, Zhongxian Lu
Summary: Decidualization of endometrial stroma is a crucial process for embryo implantation. In this study, the researchers investigated the transcriptional mechanism of the PTPN11 gene, which encodes the SHP2 protein involved in decidualization. They found that progesterone receptor (PGR) and cAMP-responsive element-binding protein 1 (CREB1) bind to the PTPN11 promoter and regulate the expression of SHP2 in response to decidual signals.
Article
Biotechnology & Applied Microbiology
Yu Chen, Song Gao, Jingwen Zhou, Weizhu Zeng
Summary: This study investigates the mechanism of Eaf3p, a chromatin regulator, in regulating nitrogen metabolism in Saccharomyces cerevisiae. The results show that Eaf3p regulates the expression of multiple nitrogen metabolic genes by modifying histone acetylation of the promoter regions via the histone acetyltransferase NuA4 complex. Eaf3p binds to the promoters of GAT1, CAN1, LYP1, GLN3, and HIP1 to regulate their expression. This research contributes to the understanding of S. cerevisiae nitrogen metabolism regulation and provides insights for future metabolic engineering and industrial applications.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Dermatology
Moamen Masalha, Iddo Z. Ben-Dov, Oren Ram, Tal Meningher, Jasmine Jacob-Hirsch, Riad Kassem, Yechezkel Sidi, Dror Avni
Summary: This study compared the H3K27Ac modification pattern between psoriatic lesions, uninvolved psoriatic skin, and healthy skin using ChIP-seq, aiming to identify new genes involved in the pathogenesis of psoriasis. The research found differential H3K27Ac patterns in psoriatic skin compared to uninvolved or healthy skin, and revealed an enrichment of H3K27Ac in overexpressed genes in psoriasis, potentially implicating the GRHL transcription factor.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Daniel Gussakovsky, Evan P. Booy, Mira J. F. Brown, Sean A. McKenna
Summary: This study establishes the role of nuclear SRP9/SRP14 in the transcriptional regulation of 7SL and BC200 RNA. Knockdown of SRP9/SRP14 affects the steady-state levels, decay rate, and transcriptional activity of 7SL and BC200 RNA. The findings demonstrate a novel nuclear function of SRP9/SRP14 in transcriptional regulation.
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Biochemistry & Molecular Biology
Lauren B. A. Woodruff, Thomas E. Gorochowski, Nicholas Roehner, Tarjei S. Mikkelsen, Douglas Densmore, D. Benjamin Gordon, Robert Nicol, Christopher A. Voigt
NUCLEIC ACIDS RESEARCH
(2017)
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Multidisciplinary Sciences
Kelley S. Yan, Claudia Y. Janda, Junlei Chang, Grace X. Y. Zheng, Kathryn A. Larkin, Vincent C. Luca, Luis A. Chia, Amanda T. Mah, Arnold Han, Jessica M. Terry, Akifumi Ootani, Kelly Roelf, Mark Lee, Jenny Yuan, Xiao Li, Christopher R. Bolen, Julie Wilhelmy, Paige S. Davies, Hiroo Ueno, Richard J. von Furstenberg, Phillip Belgrader, Solongo B. Ziraldo, Heather Ordonez, Susan J. Henning, Melissa H. Wong, Michael P. Snyder, Irving L. Weissman, Aaron J. Hsueh, Tarjei S. Mikkelsen, K. Christopher Garcia, Calvin J. Kuo
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Multidisciplinary Sciences
Sharon R. Grossman, Xiaolan Zhang, Li Wang, Jesse Engreitz, Alexandre Melnikov, Peter Rogov, Ryan Tewhey, Alina Isakova, Bart Deplancke, Bradley E. Bernstein, Tarjei S. Mikkelsen, Eric S. Lander
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Stefan Semrau, Johanna E. Goldmann, Magali Soumillon, Tarjei S. Mikkelsen, Rudolf Jaenisch, Alexander van Oudenaarden
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Stephanie Kilens, Dimitri Meistermann, Diego Moreno, Caroline Chariau, Anne Gaignerie, Arnaud Reignier, Yohann Lelievre, Miguel Casanova, Celine Vallot, Steven Nedellec, Lea Flippe, Julie Firmin, Juan Song, Eric Charpentier, Jenna Lammers, Audrey Donnart, Nadege Marec, Wallid Deb, Audrey Bihouee, Cedric Le Caignec, Claire Pecqueur, Richard Redon, Paul Barriere, Jeremie Bourdon, Vincent Pasque, Magali Soumillon, Tarjei S. Mikkelsen, Claire Rougeulle, Thomas Freour, Laurent David
NATURE COMMUNICATIONS
(2018)
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Critical Care Medicine
Peter J. Castaldi, Feng Guo, Dandi Qiao, Fei Du, Zun Zar Chi Naing, Yan Li, Betty Pham, Tarjei S. Mikkelsen, Michael H. Cho, Edwin K. Silverman, Xiaobo Zhou
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Davide Cacchiarelli, Xiaojie Qiu, Sanjay Srivatsan, Anna Manfredi, Michael Ziller, Eliah Overbey, Antonio Grimaldi, Jonna Grimsby, Prapti Pokharel, Kenneth J. Livak, Shuqiang Li, Alexander Meissner, Tarjei S. Mikkelsen, John L. Rinn, Cole Trapnell
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Multidisciplinary Sciences
Quinlan L. Sievers, Georg Petzold, Richard D. Bunker, Aline Renneville, Mikolaj Slabicki, Brian J. Liddicoat, Wassim Abdulrahman, Tarjei Mikkelsen, Benjamin L. Ebert, Nicolas H. Thoma
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Developmental Biology
Alexander M. Tsankov, Marc H. Wadsworth, Veronika Akopian, Jocelyn Charlton, Samuel J. Allon, Aleksandra Arczewska, Benjamin E. Mead, Riley S. Drake, Zachary D. Smith, Tarjei S. Mikkelsen, Alex K. Shalek, Alexander Meissner
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Biochemistry & Molecular Biology
N. S. Persky, D. Hernandez, M. Do Carmo, L. Brenan, O. Cohen, S. Kitajima, U. Nayar, A. Walker, S. Pantel, Y. Lee, J. Cordova, M. Sathappa, C. Zhu, T. K. Hayes, P. Ram, P. Pancholi, T. S. Mikkelsen, D. A. Barbie, X. Yang, R. Haq, F. Piccioni, D. E. Root, C. M. Johannessen
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
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Biotechnology & Applied Microbiology
Joseph M. Replogle, Thomas M. Norman, Albert Xu, Jeffrey A. Hussmann, Jin Chen, J. Zachery Cogan, Elliott J. Meer, Jessica M. Terry, Daniel P. Riordan, Niranjan Srinivas, Ian T. Fiddes, Joseph G. Arthur, Luigi J. Alvarado, Katherine A. Pfeiffer, Tarjei S. Mikkelsen, Jonathan S. Weissman, Britt Adamson
NATURE BIOTECHNOLOGY
(2020)
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Multidisciplinary Sciences
Ameen A. Salahudeen, Shannon S. Choi, Arjun Rustagi, Junjie Zhu, Vincent van Unen, Sean M. De la O, Ryan A. Flynn, Mar Margalef-Catala, Antonio J. M. Santos, Jihang Ju, Arpit Batish, Tatsuya Usui, Grace X. Y. Zheng, Caitlin E. Edwards, Lisa E. Wagar, Vincent Luca, Benedict Anchang, Monica Nagendran, Khanh Nguyen, Daniel J. Hart, Jessica M. Terry, Phillip Belgrader, Solongo B. Ziraldo, Tarjei S. Mikkelsen, Pehr B. Harbury, Jeffrey S. Glenn, K. Christopher Garcia, Mark M. Davis, Ralph S. Baric, Chiara Sabatti, Manuel R. Amieva, Catherine A. Blish, Tushar J. Desai, Calvin J. Kuo
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Biochemistry & Molecular Biology
Motohiko Kadoki, Ashwini Patil, Cornelius C. Thaiss, Donald J. Brooks, Surya Pandey, Deeksha Deep, David Alvarez, Ulrich H. von Andrian, Amy J. Wagers, Kenta Nakai, Tarjei S. Mikkelsen, Magali Soumillon, Nicolas Chevrier
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Cell & Tissue Engineering
Kelley S. Yan, Olivier Gevaert, Grace X. Y. Zheng, Benedict Anchang, Christopher S. Probert, Kathryn A. Larkin, Paige S. Davies, Zhuan-fen Cheng, John S. Kaddis, Arnold Han, Kelly Roelf, Ruben I. Calderon, Esther Cynn, Xiaoyi Hu, Komal Mandleywala, Julie Wilhelmy, Sue M. Grimes, David C. Corney, Stephane C. Boutet, Jessica M. Terry, Phillip Belgrader, Solongo B. Ziraldo, Tarjei S. Mikkelsen, Fengchao Wang, Richard J. von Furstenberg, Nicholas R. Smith, Parthasarathy Chandrakesan, Randal May, Mary Ann S. Chrissy, Rajan Jain, Christine A. Cartwright, Joyce C. Niland, Young-Kwon Hong, Jill Carrington, David T. Breault, Jonathan Epstein, Courtney W. Houchen, John P. Lynch, Martin G. Martin, Sylvia K. Plevritis, Christina Curtis, Hanlee P. Ji, Linheng Li, Susan J. Henning, Melissa H. Wong, Calvin J. Kuo
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Cell & Tissue Engineering
Evanthia E. Pashos, Yoson Park, Xiao Wang, Avanthi Raghavan, Wenli Yang, Deepti Abbey, Derek T. Peters, Juan Arbelaez, Mayda Hernandez, Nicolas Kuperwasser, Wenjun Li, Zhaorui Lian, Ying Liu, Wenjian Lv, Stacey L. Lytle-Gabbin, Dawn H. Marchadier, Peter Rogov, Jianting Shi, Katherine J. Slovik, Ioannis M. Stylianou, Li Wang, Ruilan Yan, Xiaolan Zhang, Sekar Kathiresan, Stephen A. Duncan, Tarjei S. Mikkelsen, Edward E. Morrisey, Daniel J. Rader, Christopher D. Brown, Kiran Musunuru