期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 288, 期 47, 页码 34205-34216出版社
ELSEVIER
DOI: 10.1074/jbc.M113.518993
关键词
Enzyme Catalysis; Parasite; Parasite Metabolism; Trypanosoma brucei; Vacuolar Acidification; Acidocalcisome; Osmoregulation; Polyphosphate; Vacuolar Transporter Chaperone
资金
- National Institutes of Health [AI077538]
- American Heart Association
Background: Polyphosphate (polyP) accumulates in an acidic calcium store named the acidocalcisome. Results: TbVtc4 is an acidocalcisome polyP kinase required for osmoregulation and virulence. Conclusion: TbVtc4 is a potential drug target in T. bruceiSignificance: This is the first demonstration of an essential polyP kinase in trypanosomes. Polyphosphate (polyP) is an anionic polymer of orthophosphate groups linked by high energy bonds that typically accumulates in acidic, calcium-rich organelles known as acidocalcisomes. PolyP synthesis in eukaryotes was unclear until it was demonstrated that the protein named Vtc4p (vacuolar transporter chaperone 4) is a long chain polyP kinase that localizes to the yeast vacuole. Here, we report that TbVtc4 (Vtc4 ortholog of Trypanosoma brucei) encodes, in contrast, a short chain polyP kinase that localizes to acidocalcisomes. The subcellular localization of TbVtc4 was demonstrated by fluorescence and electron microscopy of cell lines expressing TbVtc4 in its endogenous locus fused to an epitope tag and by purified polyclonal antibodies against TbVtc4. Recombinant TbVtc4 was expressed in bacteria, and polyP kinase activity was assayed in vitro. The in vitro growth of conditional knock-out bloodstream form trypanosomes (TbVtc4-KO) was significantly affected relative to the parental cell line. This mutant had reduced polyP kinase activity and short chain polyP content and was considerably less virulent in mice. The wild-type phenotype was recovered when an ectopic copy of the TbVtc4 gene was expressed in the presence of doxycycline. The mutant also exhibited a defect in volume recovery under osmotic stress conditions in vitro, underscoring the relevance of polyP in osmoregulation.
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