期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 289, 期 5, 页码 3026-3039出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.495671
关键词
Deubiquitination; E3 Ubiquitin Ligase; Endocytosis; Endosomes; Protein Sorting; Ubiquitination; ESCRT; USP8
资金
- National Institutes of Health [MH58920, CA166749]
- Discovery Fellowship from the Russell and Diana Hawkins Family Foundation
Background: Membrane protein ubiquitination is required for endosomal sorting and degradation. Results: UBE4B binds endosomes and ubiquitinates the EGFR, enabling its degradation. Conclusion: UBE4B regulates EGF receptor sorting, degradation, expression, and signaling. Significance: UBE4B couples ubiquitination and sorting machineries on endosomes and establishes a role for an endosome-associated ubiquitin ligase as crucial mediator of sorting and degradation of a membrane protein. The signaling of plasma membrane proteins is tuned by internalization and sorting in the endocytic pathway prior to recycling or degradation in lysosomes. Ubiquitin modification allows recognition and association of cargo with endosomally associated protein complexes, enabling sorting of proteins to be degraded from those to be recycled. The mechanism that provides coordination between the cellular machineries that mediate ubiquitination and endosomal sorting is unknown. We report that the ubiquitin ligase UBE4B is recruited to endosomes in response to epidermal growth factor receptor (EGFR) activation by binding to Hrs, a key component of endosomal sorting complex required for transport (ESCRT) 0. We identify the EGFR as a substrate for UBE4B, establish UBE4B as a regulator of EGFR degradation, and describe a mechanism by which UBE4B regulates endosomal sorting, affecting cellular levels of the EGFR and its downstream signaling. We propose a model in which the coordinated action of UBE4B, ESCRT-0, and the deubiquitinating enzyme USP8 enable the endosomal sorting and lysosomal degradation of the EGFR.
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