Article
Multidisciplinary Sciences
Vincent Chaptal, Veronica Zampieri, Benjamin Wiseman, Cedric Orelle, Juliette Martin, Kim-Anh Nguyen, Alexia Gobet, Margot Di Cesare, Sandrine Magnard, Waqas Javed, Jad Eid, Arnaud Kilburg, Marine Peuchmaur, Julien Marcoux, Luca Monticelli, Martin Hogbom, Guy Schoehn, Jean-Michel Jault, Ahcene Boumendjel, Pierre Falson
Summary: This study resolved three outward-facing conformations of BmrA, a multidrug ABC transporter, using x-ray crystallography and cryo-electron microscopy. It was found that two R6G molecules bind to the drug-binding cavity, inducing a rearrangement of TM1-2 and highlighting local flexibility. Simulations showed that in the absence of R6G, the cavity quickly closed after drug release, while in the presence of R6G, the cavity remained open.
Article
Biology
Sang Soo Lee, Jun Gyou Park, Eunhong Jang, Seung Hun Choi, Subin Kim, Ji Won Kim, Mi Sun Jin
Summary: The W546A mutation in Human ATP-binding cassette transporter subfamily B6 (ABCB6) alters the conformational dynamics, resulting in reduced substrate affinity and facilitating its release from the transporter.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Latifa Alketbi, Abeer Al-Ali, Iman M. Talaat, Qutayba Hamid, Khuloud Bajbouj
Summary: Colorectal cancer (CRC) is a common malignancy worldwide, ranked fourth in cancer-related deaths. Mutations in oncogenes, tumor suppressor genes, and DNA repair genes contribute to the development of CRC. Chemoresistance is one of the major challenges in CRC treatment, and the overexpression of ATP-binding cassette (ABC) transporters has been identified as a facilitating mechanism for chemoresistance. ABC transporters are involved in lipid transport and homeostasis, which play a crucial role in tumor development and progression. Altered expression of ABCA transporters in CRC is associated with poor prognosis and overall survival. This minireview suggests that ABCA transporters could serve as potential diagnostic and therapeutic targets in CRC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Andrzej Harris, Manuel Wagner, Dijun Du, Stefanie Raschka, Lea-Marie Nentwig, Holger Gohlke, Sander H. J. Smits, Ben F. Luisi, Lutz Schmitt
Summary: Pdr5, a member of the ABC transporter superfamily, plays a crucial role in drug efflux through an ATP-driven conformational cycle. The structural analysis reveals that while half of the transporter remains unchanged throughout the cycle, its partner undergoes changes to support a peristaltic motion for pumping molecules.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jingxi Liang, Xiuna Yang, Tianyu Hu, Yan Gao, Qi Yang, Haitao Yang, Wei Peng, Xiaoting Zhou, Luke W. Guddat, Bing Zhang, Zihe Rao, Fengjiang Liu
Summary: This study used single particle cryo-electron microscopy (cryo-EM) to determine the structure of the trehalose-specific transporter in Mycobacterium tuberculosis and demonstrated the potential of this technique in exploring dynamic structures of transporters. It provides insights into the trehalose recycling mechanism in Mycobacterium tuberculosis.
Article
Biochemistry & Molecular Biology
Hannah Domgaard, Christian Cahoon, Matthew J. Armbrust, Olivine Redman, Alivia Jolley, Aaron Thomas, Ryan N. Jackson
Summary: CRISPR-associated DinG protein (CasDinG) from Pseudomonas aeruginosa strain 83 is an ATP-dependent 5'-3' DNA translocase that unwinds double-stranded DNA and RNA/DNA hybrids. The crystal structure of CasDinG reveals a helicase core with three accessory domains. Deletion mutant assays demonstrate that all three domains are essential for type IV-A immunity.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Yubing Dong, Yi Lin, Wanyu Liu, Wei Zhang, Yinong Jiang, Wei Song
Summary: The study investigated the lipid profile in hypertensive disorders of pregnancy (HDP) patients, finding significantly altered levels of plasma triglycerides, total cholesterol, LDL-C, and HDL-C compared to normotensive controls. ANP was shown to inhibit ABCA1 and ABCG1 expression at both mRNA and protein levels, leading to impaired cholesterol efflux, possibly explaining the decreased HDL-C levels in HDP patients.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Dermatology
Takuya Takeichi, Takahiro Hamada, Mayuko Yamamoto, Yasutoshi Ito, Aya Kawaguchi, Haruka Kobashi, Takenori Yoshikawa, Hiroshi Koga, Norito Ishii, Takekuni Nakama, Yoshinao Muro, Tomoo Ogi, Masashi Akiyama
Summary: Pathogenic variants in the ABCA12 gene are important for the development of autosomal recessive congenital ichthyoses. In addition, these variants can also cause a non-congenital form of ichthyosis. This study found patients who carried ABCA12 variants but did not exhibit the typical congenital ichthyosis phenotype. These patients had unaffected geographic areas on their skin, suggesting a milder phenotype.
JOURNAL OF DERMATOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Genki Ichihara, Yoshinori Katsumata, Yuki Sugiura, Yuta Matsuoka, Rae Maeda, Jin Endo, Atsushi Anzai, Kohsuke Shirakawa, Hidenori Moriyama, Hiroki Kitakata, Takahiro Hiraide, Shinichi Goto, Seien Ko, Yuji Iwasawa, Kazuhisa Sugai, Kyohei Daigo, Shinya Goto, Kazuki Sato, Ken-ichi Yamada, Makoto Suematsu, Masaki Ieda, Motoaki Sano
Summary: This study found that glutathione release during ischemia-reperfusion (IR) is primarily dependent on the transporter MRP1, and its release induces ferroptosis. Suppression of glutathione release attenuates ferroptosis and reduces myocardial infarct size following IR.
CIRCULATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Su Mon Shwe, Sivaprasath Prabu, Dapeng Jing, Kanglai He, Zhenying Wang
Summary: The Cry1Ah and Vip3Aa19 proteins have a synergistic interaction in controlling the Yellow peach moth. Cry1Ah has a higher binding affinity for midgut receptors compared to Vip3Aa19, and there is no competition between them.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Silpa Narayanan, Nehaben A. Gujarati, Jing-Quan Wang, Zhuo-Xun Wu, Jagadish Koya, Qingbin Cui, Vijaya L. Korlipara, Charles R. Ashby, Zhe-Sheng Chen
Summary: The study showed that the novel benzamide derivative VKNG-2 can reverse MDR caused by overexpression of ABCG2 transporter in colon cancer cells; VKNG-2 significantly improves the efficacy of anticancer drugs in vitro and works by inhibiting the efflux function of the ABCG2 transporter.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Mengchen Shi, Yu Tian, Lingyuan He, Jingdan Zhang, Xiangling Yang, Huanliang Liu
Summary: This study evaluated the serum levels of nucleotide-metabolizing enzymes in patients with colorectal cancer and found that ATPase and AMPase activities were significantly higher in colorectal cancer patients, providing considerable diagnostic significance. The combination of CEA, ATPase, and AMPase could be a novel approach for colorectal cancer screening.
Article
Plant Sciences
Fiza Liaquat, Muhammad Farooq Hussain Munis, Samiah Arif, Muhammad Aamir Manzoor, Urooj Haroon, Iftikhar Hussain Shah, Muhammad Ashraf, Hyun Seok Kim, Shengquan Che, Liu Qunlu
Summary: This study investigated the differential gene expression of Schima superba under Mn metal stress through transcriptomic analysis. The differentially expressed genes were found to be involved in defense, signaling pathways, oxidative burst, transcription factors, and stress responses. The results provide insights into the metal resistance mechanisms and contribute to the understanding of heavy metal tolerance and detoxification.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Rossana Visigalli, Bianca Maria Rotoli, Francesca Ferrari, Maria Di Lascia, Benedetta Riccardi, Paola Puccini, Valeria Dall'Asta, Amelia Barilli
Summary: In this study, the expression and activity of ABC transporters in human alveoli were analyzed. The results showed that MRP1 was the only transporter expressed and functional at the basolateral side, while the other studied transporters were not expressed on the apical membrane. This suggests the presence of unidentified transporters involved in drug efflux from alveolar epithelium.
Article
Biochemistry & Molecular Biology
Yaolei Mi, Xue Cao, Xuewen Zhu, Weiqiang Chen, Xiangxiao Meng, Huihua Wan, Wei Sun, Sifan Wang, Shilin Chen
Summary: This study identified and characterized 113 ABC transporters in C. sativa, and proposed seven core transporters that may participate in cannabinoid transport. These candidate genes showed high correlation with cannabinoid biosynthetic pathway genes and content, and were highly expressed in cannabinoid biosynthesis and accumulation sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Gregory A. Fendley, Ina L. Urbatsch, Roger B. Sutton, Maria E. Zoghbi, Guillermo A. Altenberg
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Maria E. Zoghbi, Rebecca S. Cooper, Guillermo A. Altenberg
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemical Research Methods
Srinivasan Krishnan, Mariana C. Fiori, Ty E. Whisenant, D. Marien Cortes, Guillermo A. Altenberg, Luis G. Cuello
Article
Biochemistry & Molecular Biology
Mariana C. Fiori, Srinivasan Krishnan, Abbey Kjellgren, Luis G. Cuello, Guillermo A. Altenberg
Article
Multidisciplinary Sciences
Swapneeta S. Date, Mariana C. Fiori, Guillermo A. Altenberg, Michaela Jansen
Review
Chemistry, Multidisciplinary
Maria E. Zoghbi, Guillermo A. Altenberg
NANOTECHNOLOGY REVIEWS
(2017)
Article
Biochemistry & Molecular Biology
Maria E. Zoghbi, Guillermo A. Altenberg
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Article
Chemistry, Medicinal
Madher N. AlFindee, Yagya P. Subedi, Mariana C. Fiori, Srinivasan Krishnan, Abbey Kjellgren, Guillermo A. Altenberg, Cheng-Wei T. Chang
ACS MEDICINAL CHEMISTRY LETTERS
(2018)
Article
Multidisciplinary Sciences
Mariana C. Fiori, Yunjiang Jiang, Guillermo A. Altenberg, Hongjun Liang
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Mariana C. Fiori, Yunjiang Jiang, Wan Zheng, Miguel Anzaldua, Mario J. Borgnia, Guillermo A. Altenberg, Hongjun Liang
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Maite R. Arana, Mariana C. Fiori, Guillermo A. Altenberg
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Mariana C. Fiori, Wan Zheng, Elizabeth Kamilar, Geuel Simiyu, Guillermo A. Altenberg, Hongjun Liang
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Medicinal
Yagya P. Subedi, Abbey Kjellgren, Paul Roberts, Heath Montgomery, Noah Thackeray, Mariana C. Fiori, Guillermo A. Altenberg, Cheng-Wei T. Chang
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Ainoa Fernandez-Olivares, Eduardo Duran-Jara, Daniel A. Verdugo, Mariana C. Fiori, Guillermo A. Altenberg, Jimmy Stehberg, Ivan Alfaro, Juan Francisco Calderon, Mauricio A. Retamal
Summary: This study investigates the role of extracellular cysteines (Cys) in the gating and function of Cx46 hemichannels. The results suggest that the replacement of extracellular Cys leads to permanently closed hemichannels and alters the selectivity and voltage dependency of the hemichannels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biology
Srinivasan Krishnan, Mariana C. Fiori, Luis G. Cuello, Guillermo A. Altenberg
YALE JOURNAL OF BIOLOGY AND MEDICINE
(2017)