Article
Multidisciplinary Sciences
Omar Sandoval-Ibanez, David Rolo, Rabea Ghandour, Alexander P. Hertle, Tegan Armarego-Marriott, Arun Sampathkumar, Reimo Zoschke, Ralph Bock
Summary: The DEIP1 protein interacts with the PetA and PetB subunits and plays an essential role in the assembly of the Cytb6f complex. This finding provides insights into the assembly pathway of a crucial complex in photosynthesis.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Chao Zhang, Xinchen Mao, Xiaoxiao Feng, Yali Sun, Zirui Wang, Jiaqi Tang, Hengxiu Yu
Summary: This study identifies the essential role of OsALB3 in chloroplast development in rice and provides clues to the conserved molecular function of ALB3 in Arabidopsis, through the investigation of the yll1 mutant phenotype.
Article
Biochemistry & Molecular Biology
Matthew S. Proctor, Lorna A. Malone, David A. Farmer, David J. K. Swainsbury, Frederick R. Hawkings, Federica Pastorelli, Thomas Z. Emrich-Mills, C. Alistair Siebert, C. Neil Hunter, Matthew P. Johnson, Andrew Hitchcock
Summary: In this study, the structure of the Synechocystis sp. PCC 6803 cytb(6)f complex in the presence and absence of PetP was determined using cryogenic electron microscopy. The results revealed that PetP interacts with cytb(6)f, affecting the structure and function of the complex, and thereby regulating the electron transfer process in photosynthesis.
BIOCHEMICAL JOURNAL
(2022)
Article
Plant Sciences
Yixin Lan, Qi Chen, Mengmeng Kong, Yanyun Liu, Ming-Ju Amy Lyu, Shahnaz Perveen, Hualing Mi
Summary: The study revealed that the PetM mutant in Arabidopsis exhibited a similar phenotype to the PetC mutant, suggesting the crucial role of PetM in maintaining the function of the cyt b(6)f complex.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Microbiology
Emily E. Whittle, Helen E. McNeil, Eleftheria Trampari, Mark Webber, Tim W. Overton, Jessica M. A. Blair
Summary: Efflux is important for maintaining low intracellular accumulation only in actively growing cells, while envelope permeability is the predominant factor in stationary-phase cells. This study suggests that antibiotics with intracellular targets may be less effective in complex infections with nongrowing or slow-growing bacteria, and efflux inhibitors may potentiate the activity of existing antibiotics in actively growing bacterial infections. The remodeling of the cell envelope prior to stationary phase could provide novel drug targets.
Review
Cell Biology
Bernhard Kadenbach
Summary: ATP serves as the universal energy currency in all living cells, mainly synthesized in mitochondria through oxidative phosphorylation. Cytochrome c oxidase (COX) acts as the regulatory center of OXPHOS, regulated by various effectors such as feedback inhibition by ATP and phosphorylation. In the relaxed state, ATP inhibition of phosphorylated and dimeric COX helps maintain a low and healthy mitochondrial membrane potential, preventing the formation of ROS.
Article
Chemistry, Physical
Fatemeh Khosravitabar, Fikret Mamedov
Summary: Periodic surges of H2 evolution were observed in the green unicellular alga Chlamydomonas reinhardtii in the presence of electron transport inhibitors. Fluorescence, thermoluminescence, and electron paramagnetic resonance spectroscopy indicate the involvement of different metabolic pathways in the H2 production.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Biochemical Research Methods
Zhen Xiao, Chengcheng Huang, Haitao Ge, Yan Wang, Xiaoxiao Duan, Gaojie Wang, Limin Zheng, Jinghui Dong, Xiahe Huang, Yuanya Zhang, Hongyu An, Wu Xu, Yingchun Wang
Summary: Using APEX2-based proximity labeling, the study identified proteins proximal to the photosystem II oxygen evolution complex (OEC) in a cyanobacterium model. Several proteins involved in PS II assembly, maturation, and repair were discovered, along with potential regulators of PS II structure and function. This study provides valuable insights into the proteome neighborhood and regulation of a key protein complex.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Agricultural Engineering
Long-Hai Zou, Ya-Qian Yang, Xuan Liu, Muthusamy Ramakrishnan, Mingbing Zhou
Summary: This study investigates the molecular basis for chlorosis in bamboo culm tissue. By analyzing gene expression and conducting experiments, the researchers identify the decreased expression of BBX15s as the underlying cause of chlorophyll deficiency and thylakoid dysgenesis in the culm, shedding light on the mechanism of moso bamboo culm chloroplast development.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Plant Sciences
Alexander N. N. Tikhonov
Summary: In oxygenic photosynthetic systems, the Cytb(6)f complex plays a crucial role in connecting photosystem II and I. This review focuses on the mechanism of the bifurcated oxidation of plastoquinol within the Cytb(6)f complex. The diffusion of plastoquinol from PSII to Cytb(6)f does not limit intersystem electron transport, and the rate of plastoquinol turnover is mainly determined by the oxidation at the catalytic site Q(o).
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Na Li, Wing Shing Wong, Lei Feng, Chunming Wang, King Shing Wong, Nianhui Zhang, Wei Yang, Yueming Jiang, Liwen Jiang, Jun-Xian He
Summary: The study reports the discovery and characterization of a new tiny albino 1 (nta1) mutant in Arabidopsis, which shows severe defects in the accumulation of cytochrome b6f (Cyt b6f) complex and chloroplast development. The nta1 phenotype is caused by disruption of a single nuclear gene, NTA1, which encodes a conserved thylakoid membrane protein. NTA1 interacts with multiple subunits of Cyt b6f and plays an essential role in its assembly and the development of chloroplast.
PLANT COMMUNICATIONS
(2023)
Article
Cell Biology
Myriam Canonico, Grzegorz Konert, Aurelie Crepin, Barbora Sediva, Radek Kana
Summary: The study investigates photoprotective mechanisms of photosynthetic organisms under high-light stress, revealing a three-phase response to acute high-light stress at the single-cell level. Carotenoid accumulation during high-light stress may be involved in direct photoprotection and optimal distribution of photosystems in the thylakoid membrane.
Article
Biotechnology & Applied Microbiology
Sergio Villicana, Juan Castillo-Fernandez, Eilis Hannon, Colette Christiansen, Pei-Chien Tsai, Jane Maddock, Diana Kuh, Matthew Suderman, Christine Power, Caroline Relton, George Ploubidis, Andrew Wong, Rebecca Hardy, Alissa Goodman, Ken K. Ong, Jordana T. Bell
Summary: This study conducted heritability and methylation quantitative trait locus (meQTL) analysis on 724,499 CpGs using the Illumina Infinium MethylationEPIC array in 2358 blood samples from three UK cohorts. They found that 34.2% of CpGs are affected by SNPs, with 98% of effects being cis-acting or within 1 Mbp of the tested CpG. They also observed overrepresentation of meQTLs in enhancers and identified co-localisations between genetic effects on DNA methylation and various human traits, including disease-relevant genes.
Review
Plant Sciences
Akiko Yoshihara, Koichi Kobayashi
Summary: This article summarizes recent high-resolution observations of lipid molecules in the structures of photosynthetic complexes from plants, algae, and cyanobacteria, and evaluates the distribution of lipids among photosynthetic protein complexes and thylakoid lipid bilayers. By integrating the structural information with the findings from biochemical and molecular genetic studies, this article highlights the conserved and differentiated roles of lipids in the assembly and functions of photosynthetic complexes among plants, algae, and cyanobacteria.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Bojian Ding, Sheng Yang, Matthias Schaks, Yijun Liu, Abbigale J. Brown, Klemens Rottner, Saikat Chowdhury, Baoyu Chen
Summary: In this study, the structures of WRC, both alone and bound to Rac1, were investigated using cryogenic-electron microscopy. The findings reveal that Rac1 binds to WRC at two distinct sites, and binding to the A site activates the complex. Activation involves conformational changes that result in the release of sequestered WCA polypeptide, stimulating actin polymerization by the Arp2/3 complex. These structural insights, combined with biochemical and cellular analyses, provide a novel understanding of the regulation of the Rac1-WRC-Arp2/3-actin signaling axis in various biological processes and diseases.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Wenqiang Yang, Tyler M. Wittkopp, Xiaobo Li, Jaruswan Warakanont, Alexandra Dubini, Claudia Catalanotti, Rick G. Kim, Eva C. M. Nowack, Luke C. M. Mackinder, Munevver Aksoy, Mark Dudley Page, Sarah D'Adamo, Shai Saroussi, Mark Heinnickel, Xenie Johnson, Pierre Richaud, Jean Alric, Marko Boehm, Martin C. Jonikas, Christoph Benning, Sabeeha S. Merchant, Matthew C. Posewitz, Arthur R. Grossman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Multidisciplinary Sciences
Donald R. Ort, Sabeeha S. Merchant, Jean Alric, Alice Barkan, Robert E. Blankenship, Ralph Bock, Roberta Croce, Maureen R. Hanson, Julian M. Hibberd, Stephen P. Long, Thomas A. Moore, James Moroney, Krishna K. Niyogi, Martin A. J. Parry, Pamela P. Peralta-Yahya, Roger C. Prince, Kevin E. Redding, Martin H. Spalding, Klaas J. van Wijk, Wim F. J. Vermaas, Susanne von Caemmerer, Andreas P. M. Weber, Todd O. Yeates, Joshua S. Yuan, Xin Guang Zhu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Plant Sciences
Jean Alric
FRONTIERS IN PLANT SCIENCE
(2015)
Review
Plant Sciences
Louis Dumas, Marie Chazaux, Gilles Peltier, Xenie Johnson, Jean Alric
PHOTOSYNTHESIS RESEARCH
(2016)
Article
Multidisciplinary Sciences
Louis Dumas, Francesca Zito, Stephanie Blangy, Pascaline Auroy, Xenie Johnson, Gilles Peltier, Jean Alric
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Plant Sciences
Brigitte Ksas, Bertrand Legeret, Ursula Ferretti, Anne Chevalier, Pavel Pospisil, Jean Alric, Michel Havaux
PLANT CELL AND ENVIRONMENT
(2018)
Article
Plant Sciences
Louis Dumas, Francesca Zito, Pascaline Auroy, Xenie Johnson, Gilles Peltier, Jean Alric
Article
Plant Sciences
Elizabeth Hughes, Jean Alric, Vladimir Yurkov
PHOTOSYNTHESIS RESEARCH
(2020)
Article
Plant Sciences
Charlie Mathiot, Jean Alric
Summary: The ECS technique, based on absorbance shift of pigments, is used for quantitative studies on photosynthetic energy transduction. It is currently utilized to monitor post-illumination decay of proton-motive force and to analyze electron transmission along the photosynthetic chain.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Brigitte Ksas, Jean Alric, Stefano Caffarri, Michel Havaux
Summary: In Arabidopsis plants, the accumulation of plastoquinone (PQ) in leaves under high light occurs mostly in the non-photoactive pool. The size of the thylakoid PQ pool and the amount of PSII reaction centers are minimally affected by light intensity. Boosting PQ biosynthesis leads to increased PQ levels, but the accumulation still occurs in the non-photoactive pool. The regulation of PQ distribution within chloroplasts is tightly controlled.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Maureen Saint-Sorny, Pawel Brzezowski, Stephanie Arrivault, Jean Alric, Xenie Johnson
Summary: This study utilized a Chlamydomonas reinhardtii RuBisCO-less mutant, Delta rbcL, to investigate carbohydrate metabolism in the absence of atmospheric carbon fixation. The results showed that in Delta rbcL, ATP is generated via photosynthetic electron transfer reactions at the onset of illumination, and metabolites of the CBB cycle are responsive to light.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Seddik Harchouni, Samantha England, Julien Vieu, Shanna Romand, Aicha Aouane, Sylvie Citerne, Bertrand Legeret, Jean Alric, Yonghua Li-Beisson, Benoit Menand, Benjamin Field
Summary: This study investigates the function of ppGpp signaling in the moss Physcomitrium patens and reveals that ppGpp accumulation inhibits chloroplast gene expression, resulting in a drop in photosynthetic capacity, but does not affect plant growth. This suggests that bryophytes and vascular plants may have different tolerances to defects in photosynthesis, potentially reflecting their different growth strategies.
Article
Biology
Pawel Brzezowski, Brigitte Ksas, Michel Havaux, Bernhard Grimm, Marie Chazaux, Gilles Peltier, Xenie Johnson, Jean Alric
COMMUNICATIONS BIOLOGY
(2019)
Correction
Multidisciplinary Sciences
Pierre Cardol, Jean Alric, Jacqueline Girard-Bascou, Fabrice Franck, Francis-Andre Wollman, Giovanni Finazzi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Review
Plant Sciences
Jean Alric, Xenie Johnson
CURRENT OPINION IN PLANT BIOLOGY
(2017)