Review
Oncology
Yiying Song, Haiting Liu, Qingqing Xian, Chengzhi Gui, Mingjie Xu, Yunying Zhou
Summary: UHRF1, a multifunctional epigenetic modifier, plays a crucial role in the occurrence and development of cancer. Understanding the regulatory mechanism of UHRF1 in different cancers helps identify targets for inhibiting its expression and function and provides insights for drug research.
Article
Microbiology
Taizhen Liang, Qiao Zhang, Ziyao Wu, Pei Chen, Yifan Huang, Shuwen Liu, Lin Li
Summary: HIV-1 latency is influenced by host factors and viral proteins, with UHRF1 playing a crucial role in modulating Tat stability. By disrupting the Tat/cyclin T1/CDK9 complex, UHRF1 suppresses HIV-1 transcription and maintains latency. This study provides new insights into controlling Tat expression through host-pathogen interaction for modulating HIV-1 latency, suggesting potential therapeutic strategies for HIV-1 patients.
Article
Fisheries
Songsong Hua, Fei Yu, Yongchuang Li, Xue Liu, Tingting Shi, Pan Zhang, Wanying Li, Wazir Ali Baloch, Binlun Yan, Huan Gao
Summary: Cloning and studying the function of the uhrf1 gene in Exopalaemon carinicauda reveals its important role in gonadal development, particularly in ovarian tissue. The gene regulates the expression of cell proliferation-related gene pcna and DNA methylation-related gene dnmt1, and plays a crucial role in oocyte proliferation and accumulation of exogenous nutrients.
AQUACULTURE REPORTS
(2023)
Editorial Material
Plant Sciences
Yusheng Zhao, Zhiyong Liu
Summary: Zhang et al. (2023) demonstrated through association analysis that the wheat E3 ubiquitin ligase TaAIRP2-1B positively regulates spike length by involving an upstream regulator, TaERF3, and a downstream target, TaHIPP3. This study provides a deeper understanding of spike architecture regulation and offers an alternative molecular module for designing wheat with increased yield potential.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Medicine, General & Internal
Xingang Zhang, Jishan Zheng, Huiqing Xu, Zhaoxin Ma
Summary: In this study, the methylation of COX26 in the cochlea damaged by intermittent hypoxia (IH) was investigated. The results showed that IH caused cochlear damage, accompanied by increased methylation of COX26 and expression of UHRF1 in the cochlea of neonatal rats. This study confirmed that UHRF1 induces COX26 methylation and aggravates the cochlear damage caused by IH.
Article
Multidisciplinary Sciences
Aurelie de Thonel, Johanna K. Ahlskog, Kevin Daupin, Veronique Dubreuil, Jeremy Berthelet, Carole Chaput, Geoffrey Pires, Camille Leonetti, Ryma Abane, Lluis Cordon Barris, Isabelle Leray, Anna L. Aalto, Sarah Naceri, Marine Cordonnier, Carene Benasolo, Matthieu Sanial, Agathe Duchateau, Anniina Vihervaara, Mikael C. Puustinen, Federico Miozzo, Patricia Fergelot, Elise Lebigot, Alain Verloes, Pierre Gressens, Didier Lacombe, Jessica Gobbo, Carmen Garrido, Sandy D. Westerheide, Laurent David, Michel Petitjean, Olivier Taboureau, Fernando Rodrigues-Lima, Sandrine Passemard, Delara Saberan-Djoneidi, Laurent Nguyen, Madeline Lancaster, Lea Sistonen, Valerie Mezger
Summary: This study reveals the contribution of a stress-responsive pathway to Rubinstein-Taybi syndrome (RSTS), indicating that impaired HSF2 acetylation due to RSTS-associated CBP/EP300 mutations alters the expression of neurodevelopmental players, leading to cell-cell adhesion defects.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Katherine I. Albanese, Marcey L. Waters
Summary: Recognition of trimethyllysine (Kme3) by reader proteins involves an aromatic cage binding mechanism, with some proteins containing a methionine (Met) residue instead of aromatic residues. Investigating the role of Met in binding Kme3, researchers found that Met contributes to binding through dispersion forces equally in both Kme3 and its neutral analog tert-butyl norleucine (tBuNle), indicating the lack of electrostatic interactions.
Article
Biochemistry & Molecular Biology
Min-Sung Lee, Sang-Ok Lee, Joonhyeok Choi, Minju Ryu, Mi-Kyung Lee, Ji-Hun Kim, Eunha Hwang, Chong-Kil Lee, Seung-Wook Chi, Kyoung-Seok Ryu
Summary: The RING domain of MUL1 mediates the ubiquitylation of the p53-transactivation domain. The study reveals the mechanism underlying the simultaneous recruitment of UBE2D2 and the substrate TAD(p53) by RING(MUL1). It was found that RING(MUL1) binding induced a closed conformation of UBE2D2 and accelerated its hydrolysis. Additionally, the binding affinity of TAD(p53) was enhanced by the complex formation of RING(MUL1) and UBE2D2.
Article
Biochemistry & Molecular Biology
Wallace H. Liu, Robert E. Miner, Brittany N. Albaugh, Gene E. Ananiev, Scott A. Wildman, John M. Denu
Summary: Chromatin abnormalities are common in cancer cells and are regulated by UHRF1. This study presents the discovery and mechanism of compounds that selectively inhibit the UHRF1-histone interaction, providing insight into the critical role of the PHD finger in driving histone interactions and suggesting a possible strategy for UHRF1-histone inhibition.
Review
Plant Sciences
Guoliang Han, Ziqi Qiao, Yuxia Li, Zongran Yang, Chengfeng Wang, Yuanyuan Zhang, Lili Liu, Baoshan Wang
Summary: RING zinc finger proteins play important roles in plant growth, development, and the response to abiotic stresses. They function as E3 ubiquitin ligases by modifying and degrading stress-related proteins in the cytoplasm and nucleus. Under abiotic stress, they may act as sensors or ABA receptors on the plasma membrane, or as transcription factors in the nucleus to regulate downstream gene expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
James Ross Terrell, Sijia Tang, Oluwafoyinsola Omobodunde Faniyi, In Ho Jeong, Jun Yin, Bhavitavya Nijampatnam, Sadanandan E. Velu, Wei Wang, Ruiwen Zhang, Ming Luo
Summary: Mouse double minute 2 homolog (MDM2) is an E3 ubiquitin-protein ligase that is involved in ubiquitin transfer and is elevated in cancer cells. This study solved crystal structures of MDM2 RING domain in complex with inhibitors Inulanolide A and MA242. The inhibitors bind at the C-terminus of MDM2 RING domain and disrupt interactions with the E2-Ubiquitin complex, inhibiting ubiquitin transfer for a broad spectrum of substrates.
Review
Biochemistry & Molecular Biology
Tianyuan Xie, Hai Qin, Zhengdong Yuan, Yiwen Zhang, Xiaoman Li, Lufeng Zheng
Summary: RING finger protein 168 (RNF168) is an E3 ubiquitin ligase that plays an important role in the DNA double-strand damage repair pathway. It has been found to be significantly involved in the occurrence and development of various cancers. Furthermore, RNF168 contributes to tumor cell drug resistance by enhancing DNA repair ability and regulating protein degradation. This paper summarizes and prospects the research progress of RNF168's structure, main functions, and its roles and mechanisms in tumorigenesis.
Article
Cell Biology
Dong Yan, Qingqing He, Lu Pei, Meihua Yang, Lifang Huang, Jianqiu Kong, Wang He, Hao Liu, Shizhong Xu, Haide Qin, Tianxin Lin, Jian Huang
Summary: In this study, the researchers identified the ubiquitin E3 ligase ANAPC11 as a potential oncogenic molecule in UBC cells. Clinical analysis showed that elevated expression of ANAPC11 was significantly correlated with advanced stage and poor outcomes in UBC patients. ANAPC11 was found to enhance the proliferation and invasiveness of UBC cells, while its knockout inhibited cell growth and metastasis. ANAPC11 was also found to increase ubiquitination of FOXO3, leading to its degradation and downstream effects on cell cycle regulation and androgen receptor signaling.
CELL DEATH & DISEASE
(2023)
Article
Medicine, Research & Experimental
Kun Lin, Shang-Hang Shen, Feng Lu, Pengfeng Zheng, Shizhong Wu, Jingwei Liao, Xiaohang Jiang, Guangming Zeng, De Wei
Summary: This study used the CRISPR system to construct an E3 ligase sgRNA library for glioma cell growth screening and found that RNF185 has a tumor-suppressive effect in glioblastoma. Further molecular mechanism studies revealed that the decreased expression of RNF185 may be related to the increased expression of miR-587 and DNA hypermethylation. This study provides important clues for understanding the pathogenesis of glioblastoma and offers a new perspective for glioblastoma treatment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Mathematical & Computational Biology
Susu Wu, Xiao Pan, Yanqun Cai, Caiqin Li
Summary: This study demonstrates that interfering with the expression of UHRF1 protein can inhibit the invasion and metastasis of human renal carcinoma cells, which may be achieved by mediating the Wnt/β-catenin signaling pathway and regulating the expression of MMP-2 and MMP-9.
COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE
(2022)