Article
Polymer Science
Yuying Liu, Jianping Gao, Lin Liu, Jiyao Kang, Xi Luo, Yingjun Kong, Guifeng Zhang
Summary: Collagen and fibronectin are crucial components of the extracellular matrix. This study isolated FN-binding peptides from degraded collagen and determined their amino acid sequences. The results showed that all FN-binding peptides contained specific amino acid sequences. Analysis using MALDI-TOF MS and DPI revealed the importance of peptide molecular weight range in FN binding. Furthermore, the hydroxylation of prolines was found to be necessary for FN binding in certain peptides.
Review
Engineering, Biomedical
Jean -Daniel Malcor, Frederic Mallein-Gerin
Summary: This review article discusses the use of triple-helical peptides as efficient tools to improve cell-biomaterial interactions in tissue engineering. Triple-helical peptides are bioactive molecules that mimic the architectural and biological properties of collagen. They have been successfully used to specifically recognize cell-surface receptors and provide cells seeded on biomaterials with controlled biological cues. Functionalization with triple-helical peptides has enabled researchers to improve cell function for regenerative medicine applications, such as tissue repair. However, this approach still remains under-explored and most functionalization strategies reported in the literature are unable to address collagen-binding receptors.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Jianghong Huang, Xiaoling Lei, Zhiwang Huang, Zhibin Rong, Haihang Li, Yixin Xie, Li Duan, Jianyi Xiong, Daping Wang, Shihui Zhu, Yujie Liang, Jianhao Wang, Jiang Xia
Summary: This study reports the development of an all-protein hydrogel material that promotes skin regeneration and wound healing with biocompatibility and degradability.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Review
Chemistry, Multidisciplinary
Ilamaran Meganathan, Mohandass Pachaiyappan, Mayilvahanan Aarthy, Janani Radhakrishnan, Smriti Mukherjee, Ganesh Shanmugam, Jingjing You, Niraikulam Ayyadurai
Summary: Collagen, with its stable triple helix structure, is the most preferred biomaterial in commercialized medical products. However, concerns on the purity, disease transmission, and reproducibility of animal derived collagen restrict its applications, demanding alternate recombinant sources. Advancements in synthetic biology and recombinant technology are being explored for improving the yield and biomimicry of recombinant collagen, offering a reliable and customizable source.
MATERIALS HORIZONS
(2022)
Article
Biochemistry & Molecular Biology
Agustina Setiawati, Sungwoo Jeong, Albertus Ivan Brilian, Sang Ho Lee, Jin-Gon Shim, Kwang-Hwan Jung, Kwanwoo Shin
Summary: In this study, a hybrid extracellular matrix (ECM) scaffold was successfully fabricated, and it was demonstrated that the fibronectin (FN)-rich hybrid ECM scaffold promoted successful settling of liver cells. This method holds promise for the fabrication of scaffolds that provide tailored cellular microenvironment for specific organs and can serve as novel pathways for organ replacement or regeneration.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Lanju Xu, Yufei Liu, Lizong Tang, Hui Xiao, Zhuo Yang, Shufang Wang
Summary: The study demonstrates that the rhCol III-EVs hydrogel enhances skin wound healing by promoting cell type transformation, cell migration, and angiogenesis, showing promising results in a diabetic rat skin injury model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Trang H. Phan, Coen Kuijl, Dung T. Huynh, Wouter S. P. Jong, Joen Luirink, Peter van Ulsen
Summary: The overproduction of the BAM complex has been found to increase the surface display of difficult-to-secrete chimeras, not only for monomeric autotransporter fusions but also for fusions to trimeric autotransporters. This beneficial effect suggests that overproduction of BAM might be an attractive strategy to improve the production of recombinant autotransporter constructs.
MICROBIAL CELL FACTORIES
(2021)
Article
Chemistry, Multidisciplinary
Yanyan Miao, Qianqian Gao, Menghan Mao, Chao Zhang, Liqun Yang, Yang Yang, Da Han
Summary: This study describes a platform for the targeted degradation of membrane-associated proteins using bispecific aptamer chimeras, which efficiently shuttle therapeutically relevant membrane proteins to lysosomes for degradation. This method provides a universal platform for biochemical research and potential therapeutics using readily synthesized aptamer materials.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Chemical
Bikram Adhikari, Benjamin S. Stinson, Matthew J. Osmond, Mina B. Pantcheva, Melissa D. Krebs
Summary: Glaucoma is the leading cause of irreversible blindness globally, impacting 80 million people. Research on culturing human TM cells on 3D biocompatible hydrogels incorporating GAGs has shown that substrate mechanical properties can affect hTM cell proliferation and fibronectin expression, providing insights into hTM cell response to changes in their microenvironment.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Tsai-Ling Hsu, Jia-Cherng Horng
Summary: The incorporation of Thp into collagen shows position-dependent effects on the stability of the triple helix structure, with a larger destabilization effect observed when Thp is substituted at the Y position. Furthermore, the oxidized derivatives of Thp also affect collagen stability, with a greater destabilization effect observed for derivatives at the Y position. Computational analysis suggests that the ease of interconversion between puckers and the twist conformation of S,S-dioxide Thp contribute to the destabilization effect at the Y position. This study provides new insights into the use of Thp and its derivatives for designing collagen-related biomaterials.
Article
Chemistry, Analytical
Ke Li, Delai Kang, Yu Li, Weizhe Zhu, Lian Zhu, Juntao Zhang, Chengzhi Xu, Benmei Wei, Haibo Wang
Summary: In this study, a quantitative determination method for collagen triple helix integrity was developed using bovine skin collagen peptide-protected Au/Ag nanoclusters as a fluorescent probe.
ANALYTICA CHIMICA ACTA
(2023)
Article
Engineering, Biomedical
Meng Zhang, Fenghua Zhao, Xue Zhang, Linda A. Brouwer, Janette K. Burgess, Martin C. Harmsen
Summary: This study found that MRC-5 fibroblasts promote collagen fiber remodeling and deposition of fibronectin in the hydrogel, leading to modifications in the ECM microenvironment and promoting vascularization.
MATERIALS TODAY BIO
(2023)
Article
Biotechnology & Applied Microbiology
Sofia V. Poimenidou, Nelly Caccia, Spiros Paramithiotis, Michel Hebraud, George-John Nychas, Panagiotis N. Skandamis
Summary: Temperature has an impact on the gene transcription of L. monocytogenes biofilms, and different genes show varied expression levels under different temperature conditions.
Article
Biotechnology & Applied Microbiology
Yunqing Dong, Weidong Zhu, Xiaoxuan Lei, Xin Luo, Qi Xiang, Xuanru Zhu, Qiao Pan, Panshi Jin, Biao Cheng
Summary: This study confirmed that recombinant human-like collagen and recombinant human-like fibronectin can effectively promote the proliferation, migration, and adhesion of fibroblast cells, and improve the healing speed and quality of acute wounds. The combination of the two proteins showed better results than individual treatments, indicating potential applications in the field of skin repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kazunori K. Fujii, Yuki Taga, Yusuke K. Takagi, Ryo Masuda, Shunji Hattori, Takaki Koide
Summary: Procollagen forms a triple helix structure in the endoplasmic reticulum with post-translational modifications enhancing its thermal stability. Collagen properties remain constant at lower temperatures, but at higher temperatures, an increase in post-translational modifications leads to higher thermal stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Mathematics, Interdisciplinary Applications
David Kaplan, Jianshen Chen, Sinan Yavuz, Weicong Lyu
Summary: The purpose of this paper is to demonstrate and evaluate the use of Bayesian dynamic borrowing in large-scale educational assessments. The results show that Bayesian dynamic borrowing performs well in cases with heterogeneous historical data compared to other methods, while it performs similarly to other methods in cases of homogeneous data.
Article
Neurosciences
Dustin M. Snapper, Bianca Reginauld, Volha Liaudanskaya, Vincent Fitzpatrick, Yeonho Kim, Irene Georgakoudi, David L. Kaplan, Aviva J. Symes
Summary: This study developed a novel approach to studying traumatic brain injury caused by primary blast and utilized a bioengineered 3D brain-like human tissue culture system to simulate blast wave effects. The findings showed cellular injury, axonal varicosities, and the release of known biomarkers after blast.
JOURNAL OF NEUROSCIENCE RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Constancio Gonzalez-Obeso, Emily Jane Hartzell, Ryan Albert Scheel, David L. Kaplan
Summary: Effective drug delivery is crucial for successful medical treatment. Silks and recombinant silks have emerged as preferred polymeric drug delivery systems due to their unique properties and tailored physicochemical characteristics.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Hematology
Ana Marin-Quilez, Christian Andrea Buduo, Lorena Diaz-Ajenjo, Vittorio Abbonante, Elena Vuelta, Paolo Maria Soprano, Cristina Miguel-Garcia, Sandra Santos-Minguez, Inmaculada Serramito-Gomez, Pedro Ruiz-Sala, Maria Jesus Penarrubia, Emilia Pardal, Jesus Maria Hernandez-Rivas, Jose Ramon Gonzalez-Porras, Ignacio Garcia-Tunon, Rocio Benito, Jose Rivera, Alessandra Balduini, Jose Maria Bastida
Summary: In this study, 4 variants affecting GALE were identified in 3 patients with severe thrombocytopenia. These patients exhibited giant and/or grey platelets, impaired platelet aggregation, and reduced secretion of alpha and dense granules. The study also revealed the critical role of GALE in the glycosylation and externalization of key proteins involved in platelet production and function, such as GPIb alpha-IX-V and mature beta 1 integrin.
Article
Multidisciplinary Sciences
Maobin Xie, Liming Lian, Xuan Mu, Zeyu Luo, Carlos Ezio Garciamendez-Mijares, Zhenrui Zhang, Arturo Lopez, Jennifer Manriquez, Xiao Kuang, Junqi Wu, Jugal Kishore Sahoo, Federico Zertuche Gonzalez, Gang Li, Guosheng Tang, Sushila Maharjan, Jie Guo, David L. Kaplan, Yu Shrike Zhang
Summary: This study demonstrates the rapid volumetric printing of unmodified silk sericin and silk fibroin inks, which exhibit shape-memory and tuneable mechanical properties. The inks are cytocompatible, providing expanded bioink libraries for volumetric additive manufacturing and offering potential for diverse biomedical applications.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Luciana d'Amone, Jugal Kishore Sahoo, Nicholas Ostrovsky-Snider, David L. Kaplan, Fiorenzo G. Omenetto
Summary: Mucus lines all surfaces of the human body not covered by skin and provides lubrication, hydration, and protection. The properties of mucus are influenced by changes in pH that may occur due to physiological conditions and pathological circumstances. Using biopolymers that can adhere to mucus in different conditions to reinforce the mucus barrier can be a useful strategy for protecting the underlying mucosae from damage.
Article
Engineering, Biomedical
Andrew J. Stout, Miriam L. Rittenberg, Michelle Shub, Michael K. Saad, Addison B. Mirliani, James Dolgin, David L. Kaplan
Summary: The development of cost-effective serum-free media is crucial for the economic viability of cultured meat. This study successfully replaced recombinant albumin, which is a high-cost component, with rapeseed protein isolate in a serum-free medium called Beefy-R. The new medium showed improved growth and maintained cell phenotype and myogenicity of bovine satellite cells, offering a sustainable and low-cost alternative for muscle stem cell culture in cultured meat production.
Review
Biotechnology & Applied Microbiology
Ying Pei, Wen Yang, Keyong Tang, David L. Kaplan
Summary: Collagen presents a hierarchical multilevel structure that achieves an ideal balance of mechanical strength and toughness. Isolation of mesostructured aggregates of collagen from biological tissues enables the construction of new collagen materials with ideal mechanical performance. The use of these mesostructural components has shown promise in various applications.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Chemistry, Applied
Qiongling Chen, Jinchuang Zhang, Yujie Zhang, Haodong Liu, Tongqing Li, Qiang Wang, David L. Kaplan
Summary: This study investigated the interactions between pea protein and fatty acids as well as the effect on protein conformational changes during high-moisture extrusion processing. The results showed that fatty acids induced the exposure of hydrophobic groups, weakened hydrogen bonds, and affected the aggregation of protein subunits. Unsaturated fatty acids limited protein chain refolding and covalent interactions. Additionally, stearic acid promoted the formation of fibrous structures.
Review
Biochemistry & Molecular Biology
Ana Marin-Quilez, Lorena Diaz-Ajenjo, Christian A. A. Di Buduo, Ana Zamora-Canovas, Maria Luisa Lozano, Rocio Benito, Jose Ramon Gonzalez-Porras, Alessandra Balduini, Jose Rivera, Jose Maria Bastida
Summary: Protein glycosylation, including sialylation, is important for various biological processes. Carbohydrate conjugation to molecules and receptors is critical for normal hematopoiesis and platelet regulation. More than 200 enzymes are involved in proper glycosylation and sialylation. Genetic alterations in GNE, SLC35A1, GALE, and B4GALT can lead to syndromic manifestations, severe inherited thrombocytopenia, and hemorrhagic complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Mario Milazzo, Vincent Fitzpatrick, Crystal E. Owens, Igor M. Carraretto, Gareth H. McKinley, David L. Kaplan, Markus J. Buehler
Summary: This study investigates the rheology, printability, and prosthetic mechanical properties of HA and HA-silk protein composites, highlighting the roles of composition and water content. The inclusion of silk improves the quality of printed items by reducing underextrusion and slumping. A printing map is constructed to guide the manufacturing of HA-based inks for biomedical applications requiring sub-millimetric features.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Pharmacology & Pharmacy
Marta G. Fuster, Mercedes G. Montalban, Imane Moulefera, Gloria Villora, David L. Kaplan
Summary: In this study, folic acid-conjugated silk nanoparticles were used to deliver the anticancer drug ibrutinib (IB) to cancer cells with folate receptor overexpression. The study confirmed that the functionalized nanoparticles were internalized by cancer cells, mediated by folate receptors. This suggests that the developed nanocarrier can enhance IB uptake in cancer cells and be used for drug targeting applications.
Review
Chemistry, Multidisciplinary
Jugal Kishore Sahoo, Onur Hasturk, Thomas Falcucci, David L. Kaplan
Summary: Silk fibroin is widely used in various medical fields such as tissue engineering, regenerative medicine, drug delivery, and medical devices. Advancements in silk chemistry and biomaterial designs have resulted in exciting tools for creating new silk-based materials and technologies. Selective chemistries can enhance or modify the properties of silk to address challenges in medically relevant materials.
NATURE REVIEWS CHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Kirsten R. Trinidad, Reina Ashizawa, Amin Nikkhah, Cameron Semper, Christian Casolaro, David L. Kaplan, Alexei Savchenko, Nicole Tichenor Blackstone
Summary: Growth factors are critical components in cultivated meat production, but their environmental impacts have not been accurately quantified in current assessments. This study conducted a life cycle assessment to evaluate the environmental impacts of recombinant growth factor production for cultivated meat applications. The results show that even in small quantities, recombinant growth factors can have significant environmental impacts. Future research should explore sustainable production methods or alternative options with lower environmental impacts.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Materials Science, Biomaterials
Gang Xu, Liying Xiao, Peng Guo, Yuanyuan Wang, Shiyu Ke, Guozhong Lyu, Xiangsheng Ding, Qiang Lu, David L. Kaplan
Summary: The study developed an assembly strategy to regulate multiple cues in the same scaffold. The results showed that the multiple cues in the scaffold could promote vascularization and accelerate wound healing.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)