期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 289, 期 1, 页码 346-357出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.526814
关键词
Glucose Transport; Hypoxia; Hypoxia-inducible factor (HIF); Kidney Metabolism; Renal Physiology; GLUT1; Renal Glucose Transporters; SGLT1; SGLT2
资金
- Programa para el Mejoramiento del Profesorado (PROMEP) [PROMEP/103.5/09/599]
- Programa Integral de Fortalecimiento Institucional (PIFI) [2009-24MSU0011E-12]
- CONACYT-Mx Fellowship [205614/200424]
In this work, we demonstrated the regulation of glucose transporters by hypoxia inducible factor-1 (HIF-1) activation in renal epithelial cells. LLC-PK1 monolayers were incubated for 1, 3, 6, or 12 h with 0% or 5% O-2 or 300 m cobalt (CoCl2). We evaluated the effects of hypoxia on the mRNA and protein expression of HIF-1 and of the glucose transporters SGLT1, SGLT2, and GLUT1. The data showed an increase in HIF-1 mRNA and protein expression under the three evaluated conditions (p < 0.05 versus t = 0). An increase in GLUT1 mRNA (12 h) and protein expression (at 3, 6, and 12 h) was observed (p < 0.05 versus t = 0). SGLT1 and SGLT2 mRNA and protein expression decreased under the three evaluated conditions (p < 0.05 versus t = 0). In conclusion, our results suggest a clear decrease in the expression of the glucose transporters SGLT1 and SGLT2 under hypoxic conditions which implies a possible correlation with increased expression of HIF-1.
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