Article
Biotechnology & Applied Microbiology
Guxin Cui, Pengpeng Li, Ruixue Wu, Huancai Lin
Summary: The study found that membrane vesicles (MVs) of Streptococcus mutans can inhibit biofilm formation of Streptococcus gordonii and Streptococcus sanguinis, revealing the important role of MVs in interspecies interactions.
Article
Immunology
Ulrike Redanz, Sylvio Redanz, Puthalayai Treerat, Sivaraman Prakasam, Li-Jung Lin, Justin Merritt, Jens Kreth
Summary: This study investigates the transition of polymicrobial communities in oral health from a homeostatic to a dysbiotic state using a multispecies biofilm model. The research highlights the host-microbiome interactions during this transition and demonstrates the variability of host cell responses between different cell lines, emphasizing the importance of relevant in vitro models in studying oral microbiome-host interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Giuseppe Pezzotti, Satomi Ofuji, Hayata Imamura, Tetsuya Adachi, Toshiro Yamamoto, Narisato Kanamura, Eriko Ohgitani, Elia Marin, Wenliang Zhu, Osam Mazda, Azusa Togo, Satoshi Kimura, Tadahisa Iwata, Hideki Shiba, Kazuhisa Ouhara, Takashi Aoki, Toshihisa Kawai
Summary: This study investigated the mechanisms of competition/coexistence between Streptococcus sanguinis and Streptococcus mutans using quantitative Raman spectroscopy and imaging. The results suggest that the two species engage in antagonistic interactions and that Streptococcus sanguinis acts to disrupt the biofilm constructed by Streptococcus mutans. The main chemical weapon used by Streptococcus sanguinis in coculture is the secretion of oxygen radicals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Xiaoying Lyu, Liang Wang, Yusen Shui, Qingsong Jiang, Lan Chen, Wen Yang, Xiaoya He, Jumei Zeng, Yuqing Li
Summary: The study found that UA exhibited significant antimicrobial activity against common oral streptococci, reduced EPS synthesis of oral streptococci, and suppressed gtf genes' expression. Furthermore, UA decreased the proportion of S. mutans in multi-species biofilms and showed low cytotoxicity against HOKs and HGEs.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Microbiology
Livia A. Alves, Geovanny C. Salvatierra, Victor A. Freitas, Jose F. Hoefling, Debora C. Bastos, Thais L. S. Araujo, Renata O. Mattos-Graner
Summary: This study explores the intra-species functional diversity of Streptococcus sanguinis to gain a better understanding of its commensal and pathogenic lifestyles. Through phenotypic screening, conserved and strain-specific functions involved in biofilm formation and persistence in oral and cardiovascular tissues were identified. The study also reveals a correlation between strain invasiveness and C3b binding.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eduardo M. Franco, Livia A. Alves, Hassan Naveed, Victor A. A. Freitas, Debora C. Bastos, Renata O. Mattos-Graner
Summary: Bacterial surface proteins assembled into amyloids play important roles in biofilm formation and host immune evasion in Streptococcus sanguinis. The study demonstrates the significance of sortase A in amyloidogenesis and reveals the correlation between amyloid production and biofilm formation as well as immune evasion phenotypes in different strains of S. sanguinis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yi-Ywan M. Chen, Hsing-Yi Wang, Chia-Hua Wu, Yu-Juan Lin, Cheng-Hsun Chiu
Summary: Although gene clusters encoding Tfp are commonly found in Streptococcus sanguinis, not all strains exhibit surface-dependent twitching motility. Tfp can serve as a ligand for host cell colonization, and Tfp retraction is essential for biofilm stability. The twitching phenotype is strain-specific and sensitive to growth media.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pakhshan Abdullah Hassan, Chiman Hameed Saeed, Sirwan Ahmed Rashid, Sawsan Mohammed Sorchee, Suhayla Hamad Shareef
Summary: This study developed a biofilm assay to investigate biofilm formation in Streptococcus sanguinis and found that certain genes may play a crucial role in the formation of biofilms in this bacteria, contributing to increased biofilm formation in gingivitis patients.
CELLULAR AND MOLECULAR BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Mengxia Gao, Jing Zuo, Yamin Shen, Shuo Yuan, Shuji Gao, Yuxin Wang, Yang Wang, Li Yi
Summary: In this study, the interaction between Streptococcus suis (S. suis) and Haemophilus parasuis (H. parasuis) was investigated during single infections and co-infections. It was found that these two pathogens compete in vitro, but synergistically increase colonization capacity after co-infection in vivo. The expression of virulence genes and susceptibility to antibiotics were also different in mixed culture compared to single culture. These findings highlight the importance of considering mixed species interactions in bacterial disease control and antibiotic treatment.
Article
Biochemistry & Molecular Biology
Muna Aqawi, Ronit Vogt Sionov, Michael Friedman, Doron Steinberg
Summary: The non-psychoactive compound cannabigerol (CBG) from Cannabis sativa L. has antibacterial and anti-biofilm activities against Streptococcus mutans. This study investigates the susceptibility of S. mutans to CBG and the effects on the QS systems ComCDE and LuxS. The results reveal that CBG affects the ComCDE and LuxS QS pathways and has potential as an anti-QS compound for S. mutans.
Article
Microbiology
Tao Gong, Xiaoya He, Jiamin Chen, Boyu Tang, Ting Zheng, Meiling Jing, Yongwang Lin, Yangyang Pan, Qizhao Ma, Yuqing Li, Xuedong Zhou
Summary: The RcrR gene plays a crucial role in the growth, biofilm formation, and multiple sugar transportation of Streptococcus mutans. Its deletion leads to impaired biofilm formation and altered expression of sugar transportation-related genes. RcrR acts as a transcription activator to regulate the two PTS systems and influences sugar transportation and biofilm formation.
Article
Microbiology
Adelaide Renard, Seydina M. Diene, Luka Courtier-Martinez, Julien Burlaud Gaillard, Houssein Gbaguidi-Haore, Laurent Mereghetti, Roland Quentin, Patrice Francois, Nathalie Van der Mee-Marquet
Summary: This study investigates the impact of CC17 Streptococcus agalactiae carrying group-A prophages on neonatal infections, revealing that the genetic features of these prophages affect host growth characteristics, cell morphology, and biofilm formation. The findings support the domestication of 12/111phiA prophage by the host and its role in cell autoaggregation, glycocalyx, and biofilm formation.
Article
Microbiology
Qiuxiang Zhang, Jiaxun Li, Wenwei Lu, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: The study demonstrated that L. plantarum CCFM8724 has the potential to prevent dental caries by regulating metabolism and gene expression during biofilm formation, leading to a reduction in the mass of mixed-species biofilm. This provides a rationale for evaluating L. plantarum CCFM8724 as a potential oral probiotic for inhibiting cariogenic pathogen biofilm formation and improving dental caries.
Article
Cell Biology
Takujiro Homma, Sho Kobayashi, Junichi Fujii
Summary: This study found that ferroptosis occurs in the absence of cystine or methionine, leading to lipid peroxidation and cell cycle arrest. Surprisingly, the simultaneous deprivation of both cystine and methionine failed to induce ferroptosis, but supplementation with S-adenosylmethionine restored ferroptosis. The results suggest that elevated lipid peroxidation products during cell cycle progression are involved in ferroptosis execution under conditions of cysteine starvation.
Article
Biotechnology & Applied Microbiology
Fei Li, Qian Hu, Fadi Chen, Jia Fu Jiang
Summary: The study demonstrates that vernalization and cold acclimation are two independent processes, with Arabidopsis plants showing three major networks related to ethylene and jasmonic acid response, cold acclimation, and chromatin modification after prolonged cold exposure.
Article
Dentistry, Oral Surgery & Medicine
X. Cheng, S. Redanz, P. Treerat, H. Qin, D. Choi, X. Zhou, X. Xu, J. Merritt, J. Kreth
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Microbiology
Katharina Kesy, Matthias Labrenz, Brittan S. Scales, Bernd Kreikemeyer, Sonja Oberbeckmann
Summary: The study found that potentially pathogenic Vibrio bacteria are early colonizers of microplastics and their colonization is dynamic and influenced by environmental factors. Vibrio abundance and composition on microplastics vary depending on nutrient levels, with higher nutrients possibly facilitating faster establishment of Vibrio populations.
Article
Microbiology
Laurin Christopher Gierse, Alexander Meene, Daniel Schultz, Theresa Schwaiger, Claudia Karte, Charlotte Schroeder, Haitao Wang, Christine Wuensche, Karen Methling, Bernd Kreikemeyer, Stephan Fuchs, Joerg Bernhardt, Doerte Becher, Michael Lalk, Tim Urich, Katharina Riedel
Article
Infectious Diseases
Paul G. Higgins, Ralf Matthias Hagen, Bernd Kreikemeyer, Philipp Warnke, Andreas Podbielski, Hagen Frickmann, Ulrike Loderstaedt
Summary: This study analyzed a broad spectrum of resistance genes in Acinetobacter baumannii isolated from war-injured patients in Northern Africa and the Middle East, providing more detailed epidemiological information about locally abundant clonal lineages and resistance mechanisms. The identified acquired carbapenem resistance genes, including bla(OXA-23), bla(OXA-58), bla(NDM-1), and bla(GES-11), indicate potential transmission events among the isolated strains. The study confirmed outbreak-associated clonal clusters among the Syrian and Afghan strains, expanding the knowledge on international clonal lineages and resistance mechanisms associated with Acinetobacter baumannii.
Article
Biochemistry & Molecular Biology
Anne Breitruck, Markus Weigel, Jacqueline Hofrichter, Kai Sempert, Claus Kerkhoff, Nooshin Mohebali, Steffen Mitzner, Torsten Hain, Bernd Kreikemeyer
Summary: The study found that prophylactic treatment with diosmectite can effectively alleviate the severity of DSS colitis, demonstrated by reduced weight loss, disease activity index, and histological damage score. However, diosmectite treatment did not have a significant impact on the expression of tight junction proteins. Additionally, the treatment altered the microbial composition associated with DSS, showing potential as a therapeutic approach for intestinal inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ulrike Redanz, Sylvio Redanz, Puthalayai Treerat, Sivaraman Prakasam, Li-Jung Lin, Justin Merritt, Jens Kreth
Summary: This study investigates the transition of polymicrobial communities in oral health from a homeostatic to a dysbiotic state using a multispecies biofilm model. The research highlights the host-microbiome interactions during this transition and demonstrates the variability of host cell responses between different cell lines, emphasizing the importance of relevant in vitro models in studying oral microbiome-host interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Sylvio Redanz, Andreas Enz, Andreas Podbielski, Philipp Warnke
Summary: Malachite green (0.2%) showed the highest bacterial recovery rates and best biofilm visualization results, suitable for smooth and rough implant surfaces. Targeted swab-sampling significantly increased bacterial recovery rate compared to a dye-free random swabbing diagnostic approach.
Review
Dermatology
Marcia S. Pereira, Sylvio Redanz, Martin A. Kriegel
Summary: The skin and gut microbiota may potentially contribute to the development of cutaneous autoimmune diseases through various mechanisms including inflammation, metabolites, immune cell responses, and antigen penetration.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Immunology
Sonja Oehmcke-Hecht, Claudia Maletzki, Surabhi Surabhi, Nikolai Siemens, Valeria Khaimov, Lisa Marie John, Sina Mariella Peter, Sven Hammerschmidt, Bernd Kreikemeyer
Summary: This study investigated the influence of Streptococcus pneumoniae on the coagulation and contact system. It was found that pneumococci impair blood coagulation by autolysis and release of pneumolysin. The lysis of tissue factor-producing mononuclear cells and their procoagulant microvesicles were identified as the main trigger for defective blood coagulation. Pneumolysin was also found to bind coagulation and contact system factors without activating them.
JOURNAL OF INNATE IMMUNITY
(2023)
Article
Biochemistry & Molecular Biology
Hagen Frickmann, Felix Weinreich, Ulrike Loderstaedt, Sven Poppert, Egbert Tannich, Jana Bull, Bernd Kreikemeyer, Israel Barrantes
Summary: Molecular diagnostic approaches are increasingly used for malaria detection in non-endemic settings, but they may overlook other pathogens associated with returnees from tropical regions. This study assessed the potential of metagenomic sequencing for identifying different Plasmodium species in malaria patients' blood samples and found that its discriminatory capability at the species level is limited.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Gina Barkowsky, Corina Abt, Irina Poehner, Adam Bieda, Sven Hammerschmidt, Anette Jacob, Bernd Kreikemeyer, Nadja Patenge
Summary: Streptococcus pneumoniae is a common cause of community-acquired pneumonia and other infectious diseases, and antibiotic resistance and strain replacement are growing problems. In this study, CPP-coupled antisense PNAs showed antimicrobial activity against S. pneumoniae, reducing the viability of the bacteria in vitro and increasing the survival rate of infected larvae in vivo. These findings suggest the potential development of efficient antisense PNAs for the treatment of S. pneumoniae infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Seiko Kubota, Masanobu Nakata, Yujiro Hirose, Masaya Yamaguchi, Bernd Kreikemeyer, Narikazu Uzawa, Tomoko Sumitomo, Shigetada Kawabata
Summary: The present study revealed the involvement of CvfA in pilus production and virulence-related phenotypes in a serotype M49 strain of S. pyogenes. Deletion of cvfA led to reduced pilus production and adherence, as well as decreased virulence factor expression. These findings highlight the importance of CvfA in the regulation of pilus production and pathogenesis in S. pyogenes.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Microbiology
Laurin Christopher Gierse, Alexander Meene, Sebastian Skorka, Fabian Cuypers, Surabhi Surabhi, Borja Ferrero-Bordera, Bernd Kreikemeyer, Doerte Becher, Sven Hammerschmidt, Nikolai Siemens, Tim Urich, Katharina Riedel
Summary: Pneumonia is one of the leading causes of death worldwide. This study investigated the impact of Streptococcus pneumoniae and influenza A virus on the structure and function of the respiratory and gastrointestinal microbiomes of mice. Specific differences were identified between bacterial and viral infections using a meta-omics approach.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Corina Abt, Lisa Marie Gerlach, Jana Bull, Anette Jacob, Bernd Kreikemeyer, Nadja Patenge
Summary: Research found that hydrophobic counterion PyB can facilitate the translocation of CPP-antisense PNAs across bacterial membrane, thus enhancing its antimicrobial activity, but PyB does not significantly affect the antimicrobial activity of CPP-conjugated PNAs in Streptococcus pneumoniae.
Article
Microbiology
Seyed Babak Loghmani, Eric Zitzow, Gene Ching Chiek Koh, Andreas Ulmer, Nadine Veith, Ruth Grosseholz, Madlen Rossnagel, Maren Loesch, Ruedi Aebersold, Bernd Kreikemeyer, Tomas Fiedler, Ursula Kummer
Summary: Lactic acid bacteria (LAB) play a significant role in biotechnology and human health, but many LAB genera have developed multidrug resistance, causing problems in controlling hospital germs. This study investigated the metabolic adaptations of Enterococcus faecalis, a common LAB causing human infections, under different environmental pH conditions. The study found that glutamine auxotrophy leads to increased energy demand.
MICROBIOLOGY SPECTRUM
(2022)