Article
Engineering, Environmental
Javier Garcia-Calleja, Thibaut Cossart, Zoyne Pedrero, Joao P. Santos, Laurent Ouerdane, Emmanuel Tessier, Vera Slaveykova, David Amouroux
Summary: Understanding the complexation of mercury (Hg) with low molecular weight (LMW) bioligands can help elucidate its speciation, and the use of isotopically labeled Hg species in cyanobacteria has provided insights into the role of intracellular biogenic ligands in the speciation of iHg and MeHg.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Plant Sciences
Henna Mustila, Dorota Muth-Pawlak, Eva-Mari Aro, Yagut Allahverdiyeva
Summary: In this study, the global proteome of Synechocystis sp. PCC 6803 under fluctuating light conditions was compared with constant light conditions. It was found that cell metabolism was clearly reprogrammed, and there were correlations between mRNA and protein levels to some extent. The data suggest that higher nitrogen assimilation is maintained for long-term acclimation to fluctuating light upon CO2 step-down in Synechocystis sp. PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Sandeesha Kodru, Laszlo Sass, Priyanka Patil, Milan Szabo, Imre Vass
Summary: In this study, a TL component similar to the plant AG band, originating from NDH-1-mediated CEF, was identified in cyanobacteria. The +40 degrees C band was most efficiently induced by FR illumination at -10 degrees C, and it was observed that low temperatures block CEF at two different sites in Synechocystis PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemical Research Methods
Maurice Mager, Hugo Pineda Hernandez, Fabian Brandenburg, Luis Lopez-Maury, Alistair J. McCormick, Dennis J. Nuernberg, Tim Orthwein, David A. Russo, Angelo Joshua Victoria, Xiaoran Wang, Julie A. Z. Zedler, Filipe Branco dos Santos, Nicolas M. Schmelling
Summary: In recent years, many new synthetic biology tools have been developed for use in cyanobacteria. However, the reported characterizations of these tools often cannot be reproduced, which greatly limits comparability of results and applicability. This study assessed the reproducibility of a standard microbiological experiment in Synechocystis sp. PCC 6803 and found significant differences in spectrophotometer measurements and growth rates between laboratories. These findings highlight the need for additional reporting requirements of growth conditions for phototrophic organisms beyond light intensity and CO2 supply.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Mathematical & Computational Biology
Amit Kugler, Karin Stensjo
Summary: Understanding energy and redox homeostasis, as well as carbon partitioning, is crucial for systems metabolic engineering of cell factories. Stoichiometric metabolic modeling and network-wide analysis revealed that the rate of NAD(P)H regeneration controls the ATP/NADPH ratio, and energy and redox balance is interconnected with carbon and nitrogen metabolism. An auxiliary pathway was also identified to support cellular redox homeostasis and ATP cycling.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2023)
Article
Plant Sciences
Yumeng Zhang, Yaqi Wang, Wei Wei, Min Wang, Shuzhao Jia, Mingkun Yang, Feng Ge
Summary: Protein homeostasis in cyanobacteria is regulated by a proteolytic complex called ClpXP, which consists of ATPase ClpX and peptidase ClpP. This study aimed to investigate the functions and regulatory networks of ClpX in Synechocystis by identifying 172 differentially expressed proteins upon clpX interruption. The results provide a comprehensive ClpX-regulated protein network and new candidates for future functional studies on ClpX in cyanobacteria.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Jimin Wang, Jinchan Liu, David A. Flesher, Krystle M. Reiss, Hao-Li Huang, Ke R. Yang, William H. Armstrong, M. R. Gunner, Victor S. Batista, Richard J. Debus, Gary W. Brudvig
Summary: The cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 has been determined at high resolution, revealing differences from thermophilic cyanobacterial PSII structures that significantly impact the understanding of PSII structure and the mechanism of water oxidation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Kaisei Maeda, Yukiko Okuda, Gen Enomoto, Satoru Watanabe, Masahiko Ikeuchi
Summary: Extracellular polysaccharides of bacteria play crucial roles in biofilm formation, stress tolerance, and infectivity, with cyanobacteria uniquely producing sulfated polysaccharides to support phototrophic biofilms. Little is known about the biosynthesis and function of these polysaccharides in cyanobacteria, but a study on Synechocystis sp. strain PCC 6803 has identified the genes responsible for the synthesis of a sulfated polysaccharide called synechan, potentially leading to insights into the functions and biotechnological applications of similar polysaccharides found in cyanobacteria genomes.
Article
Microbiology
Dorota Muth-Pawlak, Sanna Kreula, Peter J. Gollan, Tuomas Huokko, Yagut Allahverdiyeva, Eva-Mari Aro
Summary: This study analyzed the proteomes of photosynthetic cyanobacteria under different conditions and found differential expression of proteins related to photosynthesis and carbon metabolism. The study also discovered unique expression patterns under specific conditions and concluded that combining photosynthetic activity with high intracellular inorganic carbon conditions promotes excellent growth in the bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Victoire Blanc-Garin, Theo Veaudor, Pierre Setif, Brigitte Gontero, Stephane D. Lemaire, Franck Chauvat, Corinne Cassier-Chauvat
Summary: This study conducted the first in vivo analysis of the canonical CP12 regulatory protein in the model cyanobacterium Synechocystis PCC 6803 and found that CP12 is essential for the catabolism of exogenously added glucose in the absence of photosynthesis. Additionally, CP12 was found to regulate the redox equilibrium of NADPH, enhancing the production of terpenes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shiqi Lin, Shiliang Li, Tong Ouyang, Gu Chen
Summary: Excess ammonium causes toxicity and stress in cyanobacteria, and how cyanobacteria respond to ammonium stress is not well understood. In this study, a gene called Slr1821 was found to be a crucial regulator for ammonium stress response in Synechocystis sp. PCC6803. The expression of many genes involved in nitrogen uptake, assimilation, and carbon regulation depended on the presence of Slr1821, highlighting its importance in maintaining carbon/nitrogen balance under high nitrogen conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Joao S. Rodrigues, Barbara Bourgade, Karen R. Galle, Pia Lindberg
Summary: In this study, the Hfq-MicC antisense RNA regulatory tool was used to target 12 genes involved in terpenoid biosynthesis, central carbon metabolism, amino acid biosynthesis, and ATP production. The effects of gene knockdown on isoprene and bisabolene production were evaluated. Six potential targets for improving terpenoid microbial production in Synechocystis were identified, and it was found that interfering with chlorophyll a and carotenoid biosynthetic pathways could enhance bisabolene production.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Takashi Inada, Shuntaro Machida, Koichiro Awai, Iwane Suzuki
Summary: Microalgal lipids and fatty acids are important components for biofuel production due to their high productivity potential. Genetic modification can lead to the synthesis of (omega-1)-hydroxy fatty acids in cyanobacteria, but high light conditions may inhibit photosynthesis and growth of the strain.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Prashant R. R. Singh, Jainendra Pathak, Rajneesh, Haseen Ahmed, Donat -P. P. Haeder, Rajeshwar P. Sinha
Summary: Cyanobacteria use circadian rhythms to cope with daily light fluctuations and the dark phase plays a significant role in protecting them from the negative impact of continuous light exposure. This study provides insights into the physiological responses of cyanobacteria to changing light environments.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Microbiology
Vanessa Krauspe, Stefan Timm, Martin Hagemann, Wolfgang R. Hess
Summary: The small protein NbID plays a crucial role in maintaining metabolic homeostasis by regulating amino acid and organic acid pools in photosynthetic cyanobacteria during nitrogen starvation. Its conservation throughout cyanobacterial radiation suggests its essential function in these organisms.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Csaba Nagy, Kati Thiel, Edita Mulaku, Henna Mustila, Paula Tamagnini, Eva-Mari Aro, Catarina C. Pacheco, Pauli Kallio
Summary: This study integrated an optimized sYFP2 expression cassette into novel sites in the chromosome and native plasmids of Synechocystis, showing similar expression levels between the different constructs. Results from RT-qPCR analysis indicated that gene expression from alternative loci is largely influenced by gene dosage in Synechocystis.
MICROBIAL CELL FACTORIES
(2021)
Article
Plant Sciences
Paul Bolay, Susan Schluter, Samuel Grimm, Matthias Riediger, Wolfgang R. Hess, Stephan Klaehn
Summary: Cyanobacteria evolved a CO2 concentrating mechanism to mitigate the ineffective fixation of CO2 by RuBisCO, however, the regulation of these carbon assimilatory systems is still not fully understood. By studying a model cyanobacterium, an essential transcriptional regulator Sll0998 was identified, which controls the activity of RuBisCO, crucial for engineering cyanobacterial cell factories.
Article
Plant Sciences
Oliver Mantovani, Viktoria Reimann, Michael Haffner, Felix Philipp Herrmann, Khaled A. Selim, Karl Forchhammer, Wolfgang R. Hess, Martin Hagemann
Summary: This study found that SbtB regulates a subset of genes involved in low carbon adaptation in Synechocystis, while c-di-AMP and cAMP have a lesser impact on gene expression under different carbon conditions.
Article
Biochemical Research Methods
Franz Opel, Nina A. Siebert, Sabine Klatt, Adrian Tuellinghoff, Janis G. Hantke, Joerg Toepel, Bruno Buehler, Dennis J. Nuernberg, Stephan Klaehn
Summary: Cyanobacteria have attracted great interest in biotechnology for their potential in sustainable, photosynthesis-driven production of fuels and value-added chemicals. However, compared to heterotrophic microorganisms, cyanobacterial model strains still lag behind in terms of molecular tools for engineering their metabolism. Researchers have developed a new tool called the pSOMA shuttle vector series for comprehensive genetic engineering of cyanobacteria and demonstrated its potential in gene expression and transfer.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Microbiology
Kuo Song, Martin Hagemann, Jens Georg, Sandra Maass, Doerte Becher, Wolfgang R. Hess
Summary: FoF1 ATP synthases produce ATP, and can operate in a reverse reaction under unfavorable conditions. The Atp Theta protein in cyanobacteria prevents the hydrolysis of ATP. Transcriptional regulation of atpT involves nucleoid-associated DNA-binding proteins and positive regulation through RpaB, while the major effect on atpT expression is mediated by controlling mRNA stability.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Paul Bolay, Luisa Hemm, Francisco J. Florencio, Wolfgang R. Hess, M. Isabel Muro-Pastor, Stephan Klaehn
Summary: This study reveals the mechanism by which cyanobacteria regulate metabolic pathways through the interaction of small protein inhibitors and sRNAs, further highlighting the importance of cyanobacteria in regulating metabolic flexibility in response to nitrogen fluctuations.
Article
Biochemical Research Methods
Tania S. Koebel, Rafael Melo Palhares, Christin Fromm, Witold Szymanski, Georgia Angelidou, Timo Glatter, Jens Georg, Bork A. Berghoff, Daniel Schindler
Summary: Synthetic biology approaches life as an engineer, using standardized and de novo design of genetic parts to build reproducible and controllable modules. Regulatory RNAs play a crucial role in bacteria, and synthetic sRNAs have broad applications in synthetic biology.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Microbiology
Mahir Bozan, Denny Popp, Rene Kallies, Ulisses Nunes da Rocha, Stephan Klaehn, Katja Buehler
Summary: Cyanobacteria have great potential in biotechnologies, and this study focuses on the development of molecular tools for genetic engineering, as well as the design of chassis strains and novel reactor concepts. The genome sequence of Tolypothrix sp. PCC 7712 is provided, and it is found to have high similarities with Tolypothrix sp. PCC 7601, but also some specific variations. These findings are important for targeted strain engineering.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemical Research Methods
Joerg Toepel, Rohan Karande, Stephan Klaehn, Bruno Buehler
Summary: Cyanobacteria have great potential to produce chemicals from sustainable resources through photosynthesis. Recent research on internal electron and carbon fluxes and their regulation provides new opportunities for efficient product formation. Novel photobioreactor concepts are being developed to ensure sufficient light supply. This review summarizes the latest developments in cyanobacterial engineering and highlights the importance of a holistic approach to establish photosynthesis-based production processes for a future green bioeconomy.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Microbiology
Franz Opel, Marvin Amadeus Itzenhaeuser, Isabel Wehner, Sara Lupacchini, Lars Lauterbach, Oliver Lenz, Stephan Klahn
Summary: Cyanobacteria have attracted attention in biotechnology for sustainable production of molecular hydrogen (H-2), but face various constraints. Researchers introduced the regulatory [NiFe]-hydrogenase from Cupriavidus necator into the cyanobacterial model strain Synechocystis sp. PCC 6803, providing the basis for the development of an intrinsic H-2 biosensor in cyanobacterial cells.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Walja C. Wanney, Loubna Youssar, Gergana Kostova, Jens Georg
Summary: We provide evidence that transcriptional interference via the collision mechanism is a prevalent mechanism for bacterial gene regulation, based on stochastic simulations and experimental data. Rifampicin time-series data can be used to globally monitor and quantify collision between sense and antisense transcription-complexes. Our findings highlight the need to consider transcript segments with varying half-lives and transcriptional properties within a single gene annotation, and we introduce the 'rifi' R-package for accurately assessing RNA stability and detecting diverse transcriptional events.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemical Research Methods
Niklas Philipp, Cedric K. Brinkmann, Jens Georg, Daniel Schindler, Bork A. Berghoff
Summary: DIGGER-Bac is a toolbox for designing and identifying seed regions and expressing synthetic sRNAs in bacteria. The SEEDling tool predicts optimal sRNA seed regions for efficient regulation of specific mRNA targets. The G-GArden tool assists with primer design for high-fidelity Golden Gate assembly of synthetic sRNA constructs.
Article
Biotechnology & Applied Microbiology
Fabian Brandenburg, Eleni Theodosiou, Carolin Bertelmann, Marcel Grund, Stephan Klaehn, Andreas Schmid, Jens O. Kroemer
Summary: Cyanobacteria, with their unique TCA cycle, have the potential to produce valuable chemicals like Hyp under photoautotrophic conditions, showing promise for increased space-time yield compared to sugar-based production in E. coli. These findings contribute to a better understanding of bioproduction from the TCA cycle in photoautotrophs and broaden the spectrum of chemicals produced in metabolically engineered cyanobacteria.
METABOLIC ENGINEERING COMMUNICATIONS
(2021)