Article
Biology
Sadek Amhaz, Batiste Boeda, Mouna Chouchene, Sabrina Colasse, Florent Dingli, Damarys Loew, Julien Henri, Celine Prunier, Laurence Levy
Summary: UBXN7 interacts with the RING domain of RNF111/RNF165 and TOPORS E3 ubiquitin ligases and regulates their activity by inhibiting E2 binding. The UAS thioredoxin-like domain of UBXN7 plays a key role in this regulation. This study reveals a novel mechanism of E3 ubiquitin ligase regulation through specific docking of a thioredoxin-like domain on RING domains.
Article
Biology
Lu Zhu, Qing Zhang, Ciro D. Cordeiro, Sudeep Banjade, Richa Sardana, Yuxin Mao, Scott D. Emr
Summary: The study reveals a novel ubiquitination modification implemented by Rsp5 adaptor proteins, which controls the recruitment and activity of Rsp5 to facilitate the turnover of membrane proteins.
Article
Oncology
Liangzi Cao, Hao Li, Xiaofang Liu, Yubang Wang, Bowen Zheng, Chengzhong Xing, Naijin Zhang, Jingwei Liu
Summary: This study investigated the molecular alterations and clinical relevance of NEDD4 E3 ligase family genes in 33 cancer types. The findings revealed increased expression of NEDD4 members in pancreatic cancer and decreased expression in thyroid cancer. NEDD4 family genes exhibited a range of mutation frequencies, with HECW1 and HECW2 showing relatively high mutation rates. Breast cancer presented with a high frequency of NEDD4 copy number amplification. Additionally, NEDD4 family members were involved in various pathways such as p53, Akt, apoptosis, and autophagy, and their expression correlated with the survival of cancer patients.
Article
Biochemistry & Molecular Biology
Maria Birkou, Vasilios Raptis, Konstantinos D. Marousis, Athanasios Tsevis, Kyriakos Bourikas, Detlef Bentrop, Vasso Episkopou, Georgios A. Spyroulias
Summary: Single nucleotide polymorphisms (SNPs) are important in human health research, and mutations in Arkadia (RNF111) can increase enzymatic activity, leading to enhanced aggressiveness and metastasis in tumor cells.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Pathology
Christina H. Stuelten, Nicolas Melis, Bhagawat Subramanian, Yi Tang, Megan Kimicata, John P. Fisher, Roberto Weigert, Ying E. Zhang
Summary: This study found that Smurf2 plays an important role in cutaneous wound healing. Loss of Smurf2 exacerbates early inflammation in the wounds and leads to narrower wounds but with greater breaking strength. In addition, loss of Smurf2 also increases the linearization of collagen bundles in normal and wounded skin.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Microbiology
Kelong Ma, Rundong Shu, Hongtao Liu, Jiaqi Fu, Zhao-Qing Luo, Jiazhang Qiu
Summary: Legionella pneumophila, a pathogenic bacteria, can create an environment conducive to its replication by exploiting host cell signaling pathways and the ubiquitin system. In this study, we identified a novel effector protein, Lug15, which functions as an E3 ligase and remodels the Legionella phagosome.
Article
Plant Sciences
Bo Lu, Jianhua Li, MingTai Gui, Lei Yao, Mingsong Fan, Xunjie Zhou, Deyu Fu
Summary: SalB protects cardiomyocytes from I/R-induced injury by regulating the TRIM8/GPX1 axis. TRIM8 overexpression promotes cardiomyocyte injury, which can be alleviated by SalB. In addition, TRIM8 negatively regulates the expression of the antioxidant enzyme GPX1.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Me-Sun Kim, Seo-Rin Ko, Yu Jin Jung, Kwon-Kyoo Kang, Yung-Jo Lee, Yong-Gu Cho
Summary: Plants produce stress-resistant substances in response to abiotic stress, and a protein conversion mechanism helps break down damaged proteins. The study focused on the function of OsPUB7 gene in Oryza sativa and found that OsPUB7 plays a role in drought and salinity stress resistance. The interaction between OsPUB7 and other stress-related genes suggests its potential as a target for breeding and future research on drought tolerance in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Hongbiao Ran, Chunyan Li, Ming Zhang, Jincheng Zhong, Hui Wang
Summary: Ubiquitination, a post-transcriptional modification, plays a crucial role in protein degradation and is involved in various physiological and pathological processes, including animal adipogenesis. The E3 ligases have been found to regulate adipogenesis, but their role in the recognition and connection between substrate and ubiquitin during ubiquitination has been overlooked. This review summarizes the regulatory and modification targets of E3 ligases in animal adipogenesis, explains the regulatory mechanisms in lipogenic pathways, and analyzes existing positive results, providing valuable research directions for further understanding the regulatory mechanisms of E3 ligases in animal adipogenesis.
Article
Chemistry, Multidisciplinary
Ayesha M. Patel, E. Neil G. Marsh
Summary: The study demonstrates that viperin activates a component of the protein ubiquitination machinery, enhancing TRAF6's ubiquitin transfer activity and increasing important polyubiquitinated forms, suggesting a potential role as an agonist of immune signaling pathways.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Huihui Liu, Simiao Liu, Hong Yu, Xiahe Huang, Yingchun Wang, Liang Jiang, Xiangbing Meng, Guifu Liu, Mingjiang Chen, Yanhui Jing, Feifei Yu, Bing Wang, Jiayang Li
Summary: In this study, the authors characterized and reconstituted the rice SCF E3 ligase in vitro, and also developed an engineered system with interchangeable F-box proteins. This work provides a powerful platform for studying the mechanisms of multisubunit SCF E3 ligases in eukaryotes.
Article
Entomology
Haiyin Li, Qin Lu, Yan Li, Yufang Yan, Zhiyong Yin, Jianjun Guo, Weihua Xu
Summary: The E3 ubiquitin ligase Smurf was found to be involved in diapause regulation in Helicoverpa armigera by modulating the TGF-beta signaling pathway. The ubiquitination of TGF beta RI and Smad2 by Smurf plays a crucial role in pupal development during diapause.
Article
Biochemistry & Molecular Biology
Wenjuan Yu, Ramesh Singh, Zhao Wang, Bert W. O'Malley, Ping Yi
Summary: TRAF4-mediated ubiquitination of IRS-1 is physiologically relevant and crucial for IGF signal transduction, as it promotes an atypical K29-linked ubiquitination at the C-terminal end of IRS-1, affecting downstream AKT and ERK phosphorylation and breast cancer cell proliferation. The nonproteolytic ubiquitination of IRS-1 is a key step in conveying IGF-1 stimulation from IGFR to IRS-1.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Francesca Munari, Luca Mollica, Carlo Valente, Francesca Parolini, Elham Ataie Kachoie, Giorgio Arrigoni, Mariapina D'Onofrio, Stefano Capaldi, Michael Assfalg
Summary: The study reveals that E3 ligase CHIP can ubiquitinate Tau protein at multiple sites, even in the absence of molecular chaperones, providing mechanistic insight into the chaperone-independent engagement of a disordered protein by its E3 ligase.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemical Research Methods
Brian D. Young, Jihui Sha, Ajay A. Vashisht, James A. Wohlschlegel
Summary: This study identified novel regulators of G beta gamma signaling in human cells using mass spectrometry, with KCTD2 and KCTD5 shown to specifically recognize G beta gamma and be involved in downstream signal transduction. These KCTD proteins act as substrate adaptors for a multisubunit CUL3-RING ubiquitin ligase, promoting monoubiquitination of G beta(1/2) lysine-23 and regulating downstream signaling. Depletion of these adaptors impairs downstream signaling in cancer cell lines.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Virology
Kubra Altinel, Kosuke Hashimoto, Yu Wei, Christine Neuveut, Ishita Gupta, Ana Maria Suzuki, Alexandre Dos Santos, Pierrick Moreau, Tian Xia, Soichi Kojima, Sachi Kato, Yasuhiro Takikawa, Isao Hidaka, Masahito Shimizu, Tomokazu Matsuura, Akihito Tsubota, Hitoshi Ikeda, Sumiko Nagoshi, Harukazu Suzuki, Marie-Louise Michel, Didier Samuel, Marie Annick Buendia, Jamila Faivre, Piero Carninci
JOURNAL OF VIROLOGY
(2016)
Article
Oncology
Yun Yang, Jin Zhang, Tian Xia, Gaiyun Li, Tao Tian, Mengchao Wang, Ruoyu Wang, Linghao Zha, Yuan Yang, Ke Lan, Weiping Zhou
Article
Multidisciplinary Sciences
Debora B. Petropolis, Daniela M. Faust, Matthieu Tolle, Lise Riviere, Tanguy Valentin, Christine Neuveut, Nora Hernandez-Cuevas, Alexandre Dufour, Jean-Christophe Olivo-Marin, Nancy Guillen
Article
Gastroenterology & Hepatology
Mathilde Dusseaux, Guillemette Masse-Ranson, Sylvie Darche, James Ahodantin, Yan Li, Oriane Fiquet, Elodie Beaumont, Pierrick Moreau, Lise Riviere, Christine Neuveut, Patrick Soussan, Philippe Roingeard, Dina Kremsdorf, James P. Di Santo, Helene Strick-Marchand
Article
Gastroenterology & Hepatology
Boris Halgand, Christophe Desterke, Lise Riviere, Guillaume Fallot, Mylene Sebagh, Julien Calderaro, Paulette Bioulac-Sage, Christine Neuveut, Marie-Annick Buendia, Didier Samuel, Cyrille Feray
Article
Fisheries
Sarkis Sarkis, Stephanie Dabo, Marie-Claude Lise, Christine Neuveut, Eliane F. Meurs, Vincent Lacoste, Anne Lavergne
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2018)
Article
Fisheries
Sarkis Sarkis, Marie-Claude Lise, Edith Darcissac, Stephanie Dabo, Marcel Falk, Laura Chaulet, Christine Neuveut, Eliane F. Meurs, Anne Lavergne, Vincent Lacoste
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2018)
Article
Multidisciplinary Sciences
Pierrick Moreau, Axel Cournac, Gianna Aurora Palumbo, Martial Marbouty, Shogofa Mortaza, Agnes Thierry, Stefano Cairo, Marc Lavigne, Romain Koszul, Christine Neuveut
NATURE COMMUNICATIONS
(2018)
Article
Pharmacology & Pharmacy
Lise Riviere, Barbara Quioc-Salomon, Guillaume Fallot, Boris Halgand, Cyrille Feray, Marie-Annick Buendia, Christine Neuveut
ANTIVIRAL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Mariano Genera, Barbara Quioc-Salomon, Antonin Nourisson, Baptiste Colcombet-Cazenave, Ahmed Haouz, Ariel Mechaly, Mariette Matondo, Magalie Duchateau, Alexander Konig, Marc P. Windisch, Christine Neuveut, Nicolas Wolff, Celia Caillet-Saguy
Summary: This study sheds light on the interaction between PTPN3 and HBc, providing insights into the role of PTPN3 in HBV infection and identifying potential human proteins that may interact with PTPN3 through proteomics analysis.
SCIENTIFIC REPORTS
(2021)
Review
Virology
Joao Diogo Dias, Nazim Sarica, Christine Neuveut
Summary: Hepatitis B virus (HBV) is a major public health concern with over 250 million people chronically infected and at high risk of liver diseases. Antiviral treatments control virus replication but cannot cure HBV due to the presence of viral circular episomal DNA in nuclei of infected cells. This DNA resists therapies and evades immune surveillance, making its eradication a prime target for HBV treatment.
Review
Virology
Joao Diogo Dias, Nazim Sarica, Axel Cournac, Romain Koszul, Christine Neuveut
Summary: Viruses that transcribe their DNA within the nucleus have to adapt to cellular mechanisms governing transcriptional regulation. This review provides an updated overview of how hepatitis B virus interacts with the cellular 3D genome and its impact on viral and cellular gene expression. The strategies employed by other DNA viruses to manipulate cellular genome spatial organization are also briefly discussed.
Article
Multidisciplinary Sciences
Zhen Ning, Xin Guo, Xiaolong Liu, Chang Lu, Aman Wang, Xiaolin Wang, Wen Wang, Huan Chen, Wangshu Qin, Xinyu Liu, Lina Zhou, Chi Ma, Jian Du, Zhikun Lin, Haifeng Luo, Wuxiyar Otkur, Huan Qi, Di Chen, Tian Xia, Jiwei Liu, Guang Tan, Guowang Xu, Hai-long Piao
Summary: This study reveals that USP22 stabilizes PPARgamma through deubiquitination, promoting lipid accumulation and hepatocellular carcinoma development.
NATURE COMMUNICATIONS
(2022)
Article
Gastroenterology & Hepatology
Jules Sotty, Pierre Bablon, Bouchra Lekbaby, Jeremy Augustin, Morgane Girier-Dufournier, Lucas Langlois, Celine Dorival, Fabrice Carrat, Stanislas Pol, Helene Fontaine, Nazim Sarica, Christine Neuveut, Chantal Housset, Dina Kremdsorf, Aurelie Schnuriger, Patrick Soussan
Summary: This study aimed to analyze the level of circulating forms of hepatitis B virus (HBV) in patients and evaluate their impact on the viral lifecycle. The results showed differences in the levels of different circulating forms among patients and their correlation with disease severity and viral replication activity. Furthermore, particles containing pregenomic RNA were found to play a crucial role in viral replication. These findings have important implications for further understanding the mechanisms of HBV infection and developing new treatment strategies.
HEPATOLOGY INTERNATIONAL
(2022)
Article
Oncology
Pei-Pei Xu, Su Zeng, Xiao-Tian Xia, Zi-Heng Ye, Mei-Fang Li, Ming-Yun Chen, Tian Xia, Jing-Jing Xu, Qiong Jiao, Liang Liu, Lian-Xi Li, Ming-Gao Guo
ENDOCRINE-RELATED CANCER
(2020)