Article
Microbiology
Anuj Tripathi, Ujjal K. Singha, Ayorinde Cooley, Taneisha Gillyard, Evan Krystofiak, Siddharth Pratap, Jamaine Davis, Minu Chaudhuri
Summary: TbTim50 is essential for mitochondrial function, regulation of kDNA replication, and the cell cycle in the bloodstream form of T. brucei. Depletion of TbTim50 results in inhibited cell growth, changes in morphology, increased AMPK phosphorylation, and upregulation of stumpy marker genes. TbTim50 is a crucial therapeutic target for African trypanosomiasis.
Article
Biochemistry & Molecular Biology
Roberto Docampo, Anibal Eugenio Vercesi
Summary: This review discusses the mechanisms of mitochondrial oxidant generation and removal, and the involvement of Ca2+ in trypanosome cell death, highlighting the need for further studies on ROS generation, defense mechanisms, and mitochondrial permeability transition pore in trypanosomatids.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Review
Biochemistry & Molecular Biology
Minu Chaudhuri, Anuj Tripathi, Fidel Soto Gonzalez
Summary: Mitochondria play crucial roles in cellular functions, with defects associated with various diseases. Tim50, a key receptor component, is involved in different cellular functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Samuel Rout, Silke Oeljeklaus, Abhijith Makki, Jan Tachezy, Bettina Warscheid, Andre Schneider
Summary: The study reveals that the mitochondrial import receptors ATOM46 and ATOM69 of Trypanosoma brucei have different substrate preferences, with ATOM46 favoring hydrophilic proteins and ATOM69 preferring hydrophobic substrates. While ATOM46 mainly uses electrostatic interactions for substrate binding, ATOM69 primarily uses hydrophobic interactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Jan Pyrih, Michael Hammond, Aline Alves, Samuel Dean, Jack Daniel Sunter, Richard John Wheeler, Keith Gull, Julius Lukes
Summary: The generated MitoTag dataset provides new insights into the mitochondrial proteome of Trypanosoma brucei, identifying previously undescribed proteins and revealing sub-compartment localization and novel metabolic pathways.
Article
Biology
Marcel G. Genge, Shalini Roy Chowdhury, Vit Dohnalek, Kaori Yunoki, Takashi Hirashima, Toshiya Endo, Pavel Dolezal, Dejana Mokranjac
Summary: This study reveals the coordination mechanism of translocation of proteins across mitochondrial membranes, and finds that both the core and PBD domains of Tim50 play essential roles in this process.
LIFE SCIENCE ALLIANCE
(2023)
Article
Microbiology
Joseph T. Smith Jr, Brianna Tylec, Arunasalam Naguleswaran, Isabel Roditi, Laurie K. Read
Summary: This study reveals the importance of mitochondrial mRNA editing in the developmental regulation of Trypanosoma brucei. The researchers found that temperature reduction and depletion of the differentiation-repressive kinase RDK1 can affect the metabolism of Trypanosoma brucei by altering the editing of mitochondrial cytochrome mRNAs.
Article
Microbiology
Kirsten J. Meyer, Theresa A. Shapiro
Summary: The study found that Hsp90 plays a central role as molecular chaperones in African trypanosomes, with unique functions in cell cycle regulation and kinetoplast DNA replication revealed through knockdown of cytosolic and mitochondrial Hsp90s.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Caroline E. Dewar, Silke Oeljeklaus, Jan Mani, Wignand W. D. Muehlhaeuser, Corinne von Kaenel, Johannes Zimmermann, Torsten Ochsenreiter, Bettina Warscheid, Andre Schneider
Summary: Mitochondrial quality control mechanism in Trypanosoma brucei is characterized by the ablation of ATOM69, which triggers a unique pathway resulting in the degradation of non-imported mitochondrial proteins. This process involves the recruitment of an unknown protein, an E3 ubiquitin ligase, and a ubiquitin-like protein (TbUbL1) and the release of TbUbL1 from the nucleus to the cytosol.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Katerina Karkali, Enrique Martin-Blanco
Summary: Although we have a better understanding of the gene networks controlling specific cell responses during developmental processes, we still need to determine how these networks cooperate and how signals become integrated. The JNK pathway, a key element in modulating cellular responses during development, interacts with additional regulators whose mechanisms remain largely unknown. In studying the regulation of puc expression, it was found that the JNK activity responds to some, but not all, specific enhancers, highlighting the complexity of puc expression regulation and the involvement of JNK activities in previously unknown processes during Drosophila development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Miriam Yague-Capilla, Victor M. Castillo-Acosta, Cristina Bosch-Navarrete, Luis Miguel Ruiz-Perez, Dolores Gonzalez-Pacanowska
Summary: The study characterized the unique trypanosomal SAMHD1 orthologue TbHD52 as essential in maintaining pyrimidine dNTP homeostasis and contributing to cellular dTTP biosynthesis. Knockout of TbHD52 results in cellular dysfunction, which can be rescued by overexpression of human dCMP deaminase.
ACS INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Fidel Soto-Gonzalez, Anuj Tripathi, Ayorinde Cooley, Victor Paromov, Tanu Rana, Minu Chaudhuri
Summary: In this study, the interaction and colocalization of TbTIM17 and TbTRAP1 in Trypanosoma brucei mitochondria were identified. TbTRAP1 plays a critical role in the refolding of TbTim17 and the assembly of the TbTIM17 complex. Knockdown of TbTRAP1 leads to reduced cell growth and decreased levels of mitochondrial complexes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Mabel Gill-Hille, Andre Wang, Monika W. Murcha
Summary: The PAM complex is essential for the import and assembly of most mitochondrial proteome. In this study, we analyzed the localization of PAM complex orthologues in Arabidopsis and found that Pam16 is exclusively targeted to mitochondria while Pam18 orthologues can be targeted to both mitochondria and plastids, similar to the interacting partner proteins Tim15 and Mge1.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Stephany M. Ramos de Dios, Jared L. Hass, Danielle L. Graham, Nivesh Kumar, Aina E. Antony, Martha D. Morton, David B. Berkowitz
Summary: The first dual-function assay for human serine racemase (hSR), which is essential for neuronal signaling and elevated in colorectal cancer, has been reported. This new NMR-based assay allows for monitoring of both a-proton exchange chemistry and fi-elimination, and it has been used to identify an inhibitor family that displays allosteric site binding behavior. Mechanistic information for hSR and a different partition ratio in the active site of the enzyme tryptophan synthase have also been revealed through this assay, demonstrating its value for studying enzyme mechanisms.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Cell Biology
Michael L. Kamradt, Catherine A. Makarewich
Summary: Mitochondrial microproteins play important roles in maintaining mitochondrial function and regulating cellular stress responses. Despite being a relatively new field, recent studies suggest that there are still many unknown functions of microproteins in the mitochondrial microproteome that require further investigation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)