Article
Cell Biology
Ying Zhang, Jing Zhu, Hao Xu, Qin Yi, Liang Yan, Liang Ye, Xinyuan Zhang, Min Xie, Bin Tan
Summary: Using real-time confocal microscopy and structured illumination microscopy, we observed the time-dependent internalization of fluorescently labeled S100B protein (S100B-Alexa488) into MSCs and its transport through endolysosomal pathways. Inhibitors and RNA interference approaches further demonstrated that clathrin and lipid rafts play important roles in the internalization of S100B-Alexa488, providing potential interventions for the safe application of MSCs in glioma therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jie Wang, Qihang Jiang, Roman Pleskot, Peter Grones, Elmehdi Bahafid, Gregoire Denay, Carlos Galvan-Ampudia, Xiangyu Xu, Michael Vandorpe, Evelien Mylle, Ive De Smet, Teva Vernoux, Ruediger Simon, Moritz K. Nowack, Daniel Van Damme
Summary: Endocytosis is important for plant response to stimuli and TPC plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of TPC subunits cause severe phenotypes, but partially functional alleles cause mild deviations. Reduced TPC-dependent endocytosis leads to hypersensitivity to CLAVATA3 peptide signaling.
Article
Cell Biology
Kumari Kamalesh, Nadav Scher, Tom Biton, Eyal D. Schejter, Ben-Zion Shilo, Ori Avinoam
Summary: This study found that vesicular membranes prevent fusion with the apical surface through actomyosin-mediated folding and retention, and isolate the membranes by crumpling to limit protein and lipid diffusion. Actomyosin contraction and membrane crumpling play key roles in recruiting endocytosis.
DEVELOPMENTAL CELL
(2021)
Article
Biophysics
Sebastian V. Morales, Ahmad Mahmood, Jacob Pollard, Janice Mayne, Daniel Figeys, Paul W. Wiseman
Summary: Membrane cholesterol-rich domains play a role in regulating the activity of low-density lipoprotein receptor (LDLR), which is involved in maintaining cellular cholesterol homeostasis. High membrane cholesterol concentrations lead to sequestration of LDLR in cholesterol-rich domains, reducing its ability to bind and internalize LDL particles. Reduced membrane cholesterol levels result in increased binding affinity between LDL and its receptor, and LDLR transits to nascent clathrin-coated domains for internalization.
BIOPHYSICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Marta Fratini, Praveen Krishnamoorthy, Irene Stenzel, Mara Riechmann, Monique Matzner, Kirsten Bacia, Mareike Heilmann, Ingo Heilmann
Summary: Pollen tube growth requires coordination of cytoskeletal dynamics and apical secretion, with the regulatory phospholipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P-2) playing a key role in influencing actin dynamics and secretion. Different pools of PtdIns(4,5)P-2 in the subapical plasma membrane were found in tobacco pollen tubes due to the activity of several PI4P 5-kinases (PIP5Ks). The localization of active PI4P 5-kinase variants in nanodomains was shown to stabilize actin dynamics, indicating a role of nanodomain-associated PtdIns(4,5)P-2 in actin regulation.
Review
Physiology
Gustavo R. Ares, Pablo A. Ortiz
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2015)
Editorial Material
Peripheral Vascular Disease
Pablo A. Ortiz
Article
Biochemistry & Molecular Biology
Paulo S. Caceres, Mariela Mendez, Mohammed Z. Haque, Pablo A. Ortiz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Peripheral Vascular Disease
Pablo D. Cabral, Nancy J. Hong, Md Abdul Hye Khan, Pablo A. Ortiz, William H. Beierwaltes, John D. Imig, Jeffrey L. Garvin
Article
Biochemistry & Molecular Biology
Paulo S. Caceres, Mariela Mendez, Pablo A. Ortiz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Editorial Material
Urology & Nephrology
Susan J. Allison
NATURE REVIEWS NEPHROLOGY
(2019)
Review
Urology & Nephrology
Paulo S. Caceres, Pablo A. Ortiz
CURRENT OPINION IN NEPHROLOGY AND HYPERTENSION
(2019)
Article
Urology & Nephrology
Paulo S. Caceres, Gina Savickas, Shannon L. Murray, Kausik Umanath, Junior Uduman, Jerry Yee, Tang-Dong Liao, Steven Bolin, Albert M. Levin, Moomal N. Khan, Sarah Sarkar, Jamie Fitzgerald, Dipak Maskey, Adrian H. Ormsby, Yuvraj Sharma, Pablo A. Ortiz
Summary: This study found that an increased viral load of SARS-CoV-2 in urine sediments from patients with COVID-19 is associated with a higher incidence of AKI and mortality.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Physiology
Mohammed Ziaul Haque, Pablo A. Ortiz
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2019)
Article
Physiology
Gustavo R. Ares, Kamal M. Kassem, Pablo A. Ortiz
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2019)
Article
Medicine, Research & Experimental
Ankita Bachhawat Jaykumar, Paulo S. Caceres, Keyona N. King-Medina, Tang-Dong Liao, Indrani Datta, Dipak Maskey, Jurgen K. Naggert, Mariela Mendez, William H. Beierwaltes, Pablo A. Ortiz
Article
Physiology
Kevin L. Gordish, Kamal M. Kassem, Pablo A. Ortiz, William H. Beierwaltes
PHYSIOLOGICAL REPORTS
(2017)
Article
Physiology
Vanesa D. Ramseyer, Pablo A. Ortiz, Oscar A. Carretero, Jeffrey L. Garvin
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2016)
Article
Physiology
Ankita Bachhawat Jaykumar, Paulo S. Caceres, Ibrahim Sablaban, Bakhos A. Tannous, Pablo A. Ortiz
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2016)
Review
Physiology
Ankita Bachhawat Jaykumar, Paulo S. Caceres, Pablo A. Ortiz
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2018)