Article
Biochemistry & Molecular Biology
Asako Goto, Daichi Egawa, Nario Tomishige, Toshiyuki Yamaji, Kentaro Shimasaki, Keigo Kumagai, Kentaro Hanada
Summary: This study identified an unknown cluster of amino acids in CERT that is involved in the regulation of CERT function, and substitutions of these amino acids with alanine can release the inhibitory effect on CERT activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tomasz H. Benedyk, Viv Connor, Eve R. Caroe, Maria Shamin, Dmitri I. Svergun, Janet E. Deane, Cy M. Jeffries, Colin M. Crump, Stephen C. Graham
Summary: Herpes simplex virus-1 (HSV-1) alters cellular membrane lipid composition during infection. This study demonstrates that a virus-encoded protein, pUL21, promotes the conversion of ceramide (Cer) to sphingomyelin (SM) by activating CERT. The study also reveals the importance of specific protein-protein interactions in HSV-1 mediated sphingolipid metabolism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Jiantong Shen, Yaqing Zhan, Qiulan He, Qiwen Deng, Kunhe Li, Shihong Wen, Wenqi Huang
Summary: Remifentanil protects the small intestine from ischemia/reperfusion injury by activating p38MAPK and mediating PDIA3-mediated antioxidant and anti-endoplasmic reticulum stress properties.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Taiyun Wang, Yanhua Zheng, Qi Tang, Songxiao Zhong, Wei Su, Binglian Zheng
Summary: Plant hormone brassinosteroids inhibit miRNA-mediated translational repression by negatively regulating the distribution of AGO1 at the endoplasmic reticulum in Arabidopsis thaliana. This affects the protein levels of miRNA target genes. The findings provide new insights into how miRNA-mediated gene silencing is regulated by plant endogenous hormones.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Takuma Kozono, Hiroyuki Sato, Wataru Okumura, Chifuyu Jogano, Miwa Tamura-Nakano, Yuki Kawamura, Jack Rohrer, Takashi Tonozuka, Atsushi Nishikawa
Summary: The N-terminal region of Jaw1 inhibits the formation of organized smooth ER (OSER) and maintains the homeostatic localization of interactors on the ER membrane by preventing aberrant oligomerization as an intrinsically disordered region (IDR).
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Rong-Ping Liu, Sheng-Yan He, Jing Wang, Xin-Qin Wang, Zhe-Long Jin, Hao Guo, Chao-Rui Wang, Yong-Nan Xu, Nam-Hyung Kim
Summary: This study found that 2,2’-4,4’-tetrabromodiphenyl ether (BDE-47), a commonly used flame retardant, has toxic effects on early porcine embryogenesis in vitro by inducing mitochondrial dysfunction and endoplasmic reticulum stress. The exposure to BDE-47 resulted in decreased blastocyst rate, altered gene expression, and damage to antioxidant capability and organelles. Reversal of the damage was observed when an endoplasmic reticulum stress inhibitor was applied. This study provides insights into the mechanisms of BDE-47-induced embryonic toxicity. Rating: 8/10
Article
Engineering, Biomedical
Doudou Hu, Hongxia Xu, Wei Zhang, Xiaodan Xu, Bing Xiao, Xueying Shi, Zhuxian Zhou, Nigel K. H. Slater, Youqing Shen, Jianbin Tang
Summary: The vanadyl nanocomplex STVN serves as a stable photothermal agent with the ability to induce immunogenic cell death, leading to systemic immune responses and effective tumor ablation. This novel nanocomplex demonstrates promising potential in improving cancer immunotherapy through enhanced photothermal therapy.
Article
Chemistry, Multidisciplinary
Lihua Luo, Zhenyu Luo, Junlei Zhang, Xu Liu, Jiaxin Huang, Sijie Wang, Hang Yin, Xuemeng Guo, Yilong Hu, Yichao Lu, Xinyu Shan, Huihui Liu, Yingying Shi, Yongzhong Du, Fuchun Yang, Jian You
Summary: This passage introduces a treatment strategy for acute lung injury/acute respiratory distress syndrome (ALI/ARDS) and pulmonary fibrosis (PF). By designing a multistage targeted nanomicelle loaded with endoplasmic reticulum stress inhibitor and anti-inflammatory drug, the balance of macrophage polarization and restoration of endoplasmic reticulum homeostasis can be achieved, alleviating inflammation and ultimately resolving ALI and preventing PF.
Article
Plant Sciences
Priya Gambhir, Utkarsh Raghuvanshi, Adwaita Prasad Parida, Stuti Kujur, Shweta Sharma, Sudhir K. Sopory, Rahul Kumar, Arun Kumar Sharma
Summary: Methylglyoxal detoxification, catalyzed by glutathione-dependent glyoxalase I and regulated by the MADS-box transcription factor RIPENING INHIBITOR, is crucial for fruit ripening. The decline of MG levels during fruit ripening is mainly mediated through a glutathione-dependent MG detoxification pathway and primarily catalyzed by a Glyoxalase I enzyme encoded by the SlGLYI4 gene. Silencing of SlGLYI4 leads to drastic MG overaccumulation at ripening stages of transgenic fruits and interferes with the ripening process.
Article
Biochemistry & Molecular Biology
Jin Hong Lim, Keunwan Park, Kyung Hwa Choi, Chan Wung Kim, Jae Ha Lee, Raymond Weicker, Cheol-Ho Pan, Seok-Mo Kim, Ki Cheong Park
Summary: Drug resistance is a major cause of therapeutic failure in refractory cancer. This study evaluated the clinical potential of candidate compounds 19 and 23 as SERCA inhibitors for the treatment of drug-resistant hepatocellular carcinoma. The results showed significant tumor shrinkage, indicating the clinical significance of targeting highly malignant cells for novel combinatorial strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ranen Aviner, Kathy H. Li, Judith Frydman, Raul Andino
Summary: This study examines how viral pathogens manipulate host polysomes to synthesize viral proteins and hinder host protein production. By analyzing the interactions between viruses and host cells, the research identifies potential targets for selective antiviral interventions through targeting specialized viral polysomes.
Article
Pharmacology & Pharmacy
Narathip Naradun, Krajang Talabnin, Kanyavee Issarangkul Na Ayuttha, Chutima Talabnin
Summary: Cholangiocarcinoma is a lethal malignancy with limited treatment options. Piperlongumine, a biologically active alkaloid, has shown potential as a therapeutic option for cholangiocarcinoma by inducing cancer cell death. Combination treatment with piperlongumine and a proteasome inhibitor further enhances the anti-cancer activity. This research provides valuable insights into the potential use of piperlongumine as an alternative therapy for cholangiocarcinoma.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Jialin Li, Kan Chen, Jianhua Huang, Dongqing Chu, Miaomiao Tian, Keqiang Huang, Chunyu Ma
Summary: Asiatic acid (AA) is shown to induce apoptotic death in tongue cancer cells in vitro and in vivo, potentially through mechanisms involving increased calcium levels and endoplasmic reticulum stress. This suggests that AA could be a promising therapeutic tool for tongue cancer treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Xin Nie, Dawo Liu, Mingjun Zheng, Xiao Li, Ouxuan Liu, Qian Guo, Liancheng Zhu, Bei Lin
Summary: HERPUD1 is highly expressed in ovarian cancer and positively correlated with the expression of Lewis y antigen. HERPUD1 promotes ovarian cancer by promoting cell proliferation, inhibiting apoptosis, affecting the cell cycle, promoting autophagy, and inhibiting multiple signaling pathways.
Article
Virology
Xiaoyi Suo, Jing Wang, Danping Wang, Guoqiang Fan, Mingjun Zhu, Baochao Fan, Xiaojing Yang, Bin Li
Summary: This study found that docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) significantly reduced the viral load of porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine delta coronavirus (PDCoV), and acted on the replication of the viruses rather than attachment and entry. The study further confirmed that DHA and EPA inhibited PEDV replication by alleviating endoplasmic reticulum stress. Meanwhile, DHA and EPA alleviated PEDV-induced inflammation and reactive oxygen species (ROS) levels, and enhanced cellular antioxidant capacity. These findings indicate that DHA and EPA have antiviral effects on porcine coronaviruses and provide a molecular basis for the development of new fatty acid-based therapies to control porcine coronavirus infection and transmission.
JOURNAL OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Prabu Paramasivam, Emmanuelle Meugnier, Kuppan Gokulakrishnan, Harish Ranjini, Lisa R. Staimez, Mary Beth Weber, K. M. Venkat Narayan, Hubert Vidal, Nikhil Tandon, Dorairaj Prabhakaran, Anjana Ranjit Mohan, Viswanathan Mohan, Sophie Rome, Muthuswamy Balasubramanyam
Summary: This study investigates the potential use of blood-derived miRNAs to monitor the effects of a lifestyle intervention to prevent diabetes development in obese Asian Indian pre-diabetic patients. The results show that certain miRNAs are modulated by the intervention and correlated with metabolic improvement, but the positive effects decline after 8 months post-intervention. However, the levels of certain miRNAs and inflammatory markers continue to be modified, indicating a potential link between miRNA levels and systemic inflammation.
Article
Biochemistry & Molecular Biology
Akiko Yamaji-Hasegawa, Motohide Murate, Takehiko Inaba, Naoshi Dohmae, Masayuki Sato, Fumihiro Fujimori, Yasushi Sako, Peter Greimel, Toshihide Kobayashi
Summary: We have identified a mushroom-derived protein, maistero-2, that specifically binds to cholesterol and can label cholesterol in cells with higher sensitivity than existing probes. Through our study, we have revealed the changes and distribution of cholesterol during neurite outgrowth.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Agathe Beaulant, Maya Dia, Bruno Pillot, Marie-Agnes Chauvin, Jingwei Ji-cao, Christine Durand, Nadia Bendridi, Stephanie Chanon, Aurelie Vieille-Marchiset, Claire Crola Da Silva, Stephanie Patouraux, Rodolphe Anty, Antonio Iannelli, Albert Tran, Philippe Gual, Hubert Vidal, Ludovic Gomez, Melanie Paillard, Jennifer Rieusset
Summary: The study reveals that ER-mitochondria miscommunication is an early trigger of hepatic insulin resistance and steatosis in obese mice, which can be improved by switching to a healthy diet. Manipulating organelle communication in the liver can impact hepatic metabolic functions.
JOURNAL OF HEPATOLOGY
(2022)
Article
Nutrition & Dietetics
Berenice Segrestin, Pauline Delage, Angeline Nemeth, Kevin Seyssel, Emmanuel Disse, Julie-Anne Nazare, Stephanie Lambert-Porcheron, Laure Meiller, Valerie Sauvinet, Stephanie Chanon, Chantal Simon, Helene Ratiney, Olivier Beuf, Francois Pralong, Naba-al-Huda Yassin, Alexia Boizot, Melanie Gachet, Kathryn J. Burton-Pimentel, Hubert Vidal, Emmanuelle Meugnier, Nathalie Vionnet, Martine Laville
Summary: This study tested the efficacy of grape polyphenol extract during high calorie-high fructose overfeeding and found that the supplementation did not have any impact on metabolic and weight changes in both men and women.
FRONTIERS IN NUTRITION
(2022)
Article
Neurosciences
Shani Blumenreich, Tamar Nehushtan, Or B. Barav, Jennifer T. Saville, Tamir Dingjan, John Hardy, Maria Fuller, Anthony H. Futerman
Summary: In the past decade, several genetic risk factors, including GBA variants, have been identified for Parkinson's Disease (PD). This study analyzes lipid levels in different brain regions of PD patients with GBA mutations and suggests that changes in ganglioside levels may contribute to the association between PD and GBA mutations.
NPJ PARKINSONS DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Toshihide Kobayashi
Summary: Our understanding of the asymmetric distribution of sphingomyelin in the plasma membrane is mainly based on the analysis of red blood cells using sphingomyelinase. However, recent studies have shown that the product of sphingomyelinase disrupts the distribution of lipids across the membrane. This has led to the development of a complementary histochemical method that combines electron microscopy and sphingomyelin-binding proteins. This review discusses the advantages and limitations of published methods for measuring the transbilayer distribution of sphingomyelin, as well as the latest findings on the proteins involved in its transbilayer movement.
EMERGING TOPICS IN LIFE SCIENCES
(2023)
Review
Clinical Neurology
Paul Clottes, Nicolas Benech, Chloe Dumot, Sophie Jarraud, Hubert Vidal, Laura Mechtouff
Summary: Despite recent advances in stroke therapy, it remains a leading cause of disability and death, necessitating the identification of new therapeutic targets. Increasingly, dysbiosis of gut microbiota has been recognized as playing a detrimental role in cardiovascular diseases, including stroke. The metabolites produced by gut microbiota, such as trimethylamine-N-oxide, short chain fatty acids, and tryptophan, have been found to be key players. There is evidence linking gut microbiota alteration to cardiovascular risk factors, supported by preclinical studies. Additionally, observational studies have shown that stroke patients with altered gut microbiota experience more complications, larger infarct sizes, and worse clinical outcomes. Various microbiota-targeted strategies, including prebiotics/probiotics and inhibitors of metabolites, have been developed. However, the timing and endpoints of these studies have varied, resulting in different outcomes. Based on available evidence, it is believed that further research on microbiota-targeted strategies, in conjunction with conventional stroke care, should be conducted within three therapeutic time windows: primary prevention or secondary prevention to control cardiovascular risk factors, acute phase to minimize infarct size and systemic complications, and subacute phase to prevent recurrence and promote neurological recovery.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Article
Multidisciplinary Sciences
Martin Schwarzer, Umesh Kumar Gautam, Kassem Makki, Anne Lambert, Tomas Brabec, Amelie Joly, Dagmar Srutkova, Pierre Poinsot, Tereza Novotna, Stephanie Geoffroy, Pascal Courtin, Petra Petr Hermanova, Renata C. Matos, Jonathan J. M. Landry, Celine Gerard, Anne-Laure Bulteau, Tomas Hudcovic, Hana Kozakova, Dominik Filipp, Marie -Pierre Chapot-Chartier, Marek Sinkora, Noel Peretti, Ivo Gomperts Boneca, Mathias Chamaillard, Hubert Vidal, Filipe De Vadder, Francois Leulier
Summary: The intestinal microbiota plays a role in postnatal growth, and Lactiplantibacillus plantarum strain LpWJL has been found to support growth in undernourished animals. This strain stimulates insulin-like growth factor-1 (IGF-1) and insulin production and activity. Cell walls from LpWJL, as well as specific molecules, can promote growth despite undernutrition. NOD2 in intestinal epithelial cells is necessary for LpWJL-mediated IGF-1 production and growth promotion in malnourished animals.
Article
Multidisciplinary Sciences
Iris D. D. Zelnik, Beatriz Mestre, Jonathan J. J. Weinstein, Tamir Dingjan, Stav Izrailov, Shifra Ben-Dor, Sarel J. J. Fleishman, Anthony H. H. Futerman
Summary: Researchers validate a one-step algorithm called mPROSS for stabilizing membrane proteins directly from an AlphaFold2 model structure. By applying this algorithm to ceramide synthase, they obtained a more stable form of human CerS2 enzyme through 37 designed mutations. With the help of molecular dynamics simulations, a potential pathway for substrate delivery to ceramide synthases is proposed.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Berengere Benoit, Alice Beau, Emilie Bres, Stephanie Chanon, Claudie Pinteur, Aurelie Vieille-Marchiset, Audrey Jalabert, Hao Zhang, Priyanka Garg, Maura Strigini, Laurence Vico, Jerome Ruzzin, Hubert Vidal, Laetitia Koppe
Summary: Chronic kidney disease is associated with osteosarcopenia, and the intestinal hormone FGF19 can promote skeletal muscle growth and improve glucose homeostasis. In a CKD mouse model, treatment with FGF19 increased muscle fiber size, reduced myostatin gene expression, improved glucose intolerance, and decreased liver inflammation markers.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Pauline Delage, Berenice Segrestin, Kevin Seyssel, Stephanie Chanon, Aurelie Vieille-Marchiset, Annie Durand, Angeline Nemeth, Sylviane Metairon, Aline Charpagne, Patrick Descombes, Jorg Hager, Martine Laville, Hubert Vidal, Emmanuelle Meugnier
Summary: The adaptive response to overfeeding involves significant changes in gene expression in adipose tissue. This study investigated whether supplementation with polyphenols could affect these molecular adaptations in lean men. The results showed that polyphenol supplementation modulated gene expression in adipose tissue and potentially protected against adipose tissue expansion during weight gain.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Shani Blumenreich, Doreen Padan Ben-Yashar, Tali Shalit, Meital Kupervaser, Ivan Milenkovic, Tammar Joseph, Anthony H. Futerman
Summary: Gaucher's disease is caused by a defective enzyme called acid beta-glucosidase. This study used a mouse model of neurological Gaucher's disease to identify differentially expressed proteins in the brain. The researchers discovered that a protein called transglutaminase 1 was highly expressed in the brains of the diseased mice. These findings provide further insights into the pathological mechanisms of neurological Gaucher's disease.
JOURNAL OF NEUROCHEMISTRY
(2023)
Correction
Multidisciplinary Sciences
Prabu Paramasivam, Emmanuelle Meugnier, Kuppan Gokulakrishnan, Harish Ranjani, Lisa R. Staimez, Mary Beth Weber, K. M. Venkat Narayan, Hubert Vidal, Nikhil Tandon, Dorairaj Prabhakaran, Ranjit Mohan Anjana, Viswanathan Mohan, Sophie Rome, Muthuswamy Balasubramanyam
Meeting Abstract
Chemistry, Applied
Marie-Caroline Michalski, Cecile Vors, Corinne Malpuech-Brugere, Dominique Rainteau, Emilie Gauliard, Hubert Vidal, Lemlih Ouchchane, Lydie Humbert, Stephanie Lambert-Porcheron
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2022)