Article
Virology
Laura E. M. Dunn, Fang Lu, Chenhe Su, Paul M. M. Lieberman, Joel D. D. Baines
Summary: The ability of EBV to switch between latent and lytic infection is crucial for its long-term persistence, yet the molecular mechanisms behind this switch remain unclear. Using PRO-Seq, the study mapped RNA Pol positioning and activity during latency and reactivation and found that CTCF regulates viral early transcripts during reactivation. This finding suggests that CTCF binding at specific regions helps maintain the accessibility of the viral genome for productive replication.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hongwoo Lee, Pil Joon Seo
Summary: HiCORE is a new method that predicts core chromatin regions with higher resolution through multiple layers of binning and integrated interacting bins, contributing to the identification of precise positions of potential genomic elements in a unbiased manner in both human and Arabidopsis genomes.
MOLECULES AND CELLS
(2021)
Review
Immunology
Xin Zhao, Shaoqi Zhu, Weiqun Peng, Hai-Hui Xue
Summary: T cells play a crucial role in defending against pathogens and malignant cells, and their development and differentiation rely on precise gene regulation. Higher-order genome organization, including chromatin loops and topologically associating domains, controls T cell gene expression. Recent studies have highlighted the role of transcription factors and CTCF in shaping chromatin architecture. This article summarizes current knowledge on the dynamic changes in genome topology during T cell development and function, providing a foundation for understanding the causal link between chromatin configuration and T cell transcriptional and functional outputs.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Guangyu Wang, Bo Xia, Man Zhou, Jie Lv, Dongyu Zhao, Yanqiang Li, Yiwen Bu, Xin Wang, John P. Cooke, Qi Cao, Min Gyu Lee, Lili Zhang, Kaifu Chen
Summary: The study explores the role of MACMIC analysis in identifying feature pairs with maximal colocalization and minimal correlation in the epigenomic landscape. It reveals a dual role of CTCF in epigenetic regulation of cell identity genes.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Review
Immunology
Venkataragavan Chandrasekaran, Nina Oparina, Maria-Jose Garcia-Bonete, Caroline Wasen, Malin C. Erlandsson, Eric Malmhall-Bah, Karin M. E. Andersson, Maja Jensen, Sofia T. Silfverswaerd, Gergely Katona, Maria I. Bokarewa
Summary: The cohesin complex plays an important role in leukocyte maturation and adaptive immunity, affecting genome regulation, hematopoiesis, adipogenesis, and inflammation. It is a key player in immune pathways and is associated with various immune-related diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Christy S. Varghese, Joanna L. Parish, Jack Ferguson
Summary: During persistent virus infections, the balance between virus replication, host-cell division, and immune evasion is crucial. Virus genomes can be maintained as episomes or integrate into the host genome, both strategies involving chromatinisation of viral DNA. Epigenetic repression of viral genes necessary for lytic replication occurs, while genes necessary for latent or persistent infection are kept active through chromatin insulation. Insulator proteins, such as CTCF, play a role in maintaining epigenetic boundaries and regulating transcription in viruses through various mechanisms, including chromatin looping and splicing manipulation.
CURRENT OPINION IN VIROLOGY
(2022)
Review
Cell Biology
Mathias Schwartz
Summary: Sequencing cancer predisposing genes in patients with a strong family history of cancer can help identify causative mutations and adjust the follow-up approach for their relatives. However, many families with a significant cancer history still remain unexplained. Chromosome conformation capture techniques have revealed the three-dimensional structure of chromatin and have enabled the discovery of new pathological mechanisms in cancer.
PHYSIOLOGICAL GENOMICS
(2022)
Article
Pediatrics
Xinrong Sun, Wenna Shen, Zhaokun Li, Wanggang Zhang
Summary: Studies have shown that CTCF is significantly upregulated in the airway epithelial cells of asthmatic patients, and it may aggravate bronchial epithelial cell injury by transcriptionally regulating Galectin-7 and activating the JNK/STAT3 axis.
PEDIATRIC PULMONOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Smitha Sivapragasam, Bastian Stark, Amanda Albrecht, Kaitlynne A. Bohm, Peng Mao, Raymond G. Emehiser, Steven A. Roberts, Patrick J. Hrdlicka, Gregory M. K. Poon, John J. Wyrick
Summary: Somatic mutations in DNA-binding sites for CTCF are elevated in many cancers, possibly due to its role in inhibiting repair of UV damage and modulating the formation of UV-induced DNA damage, leading to mutation hot spots. Analysis demonstrates that CTCF interacts with damaged DNA and hinders repair, providing insights into the molecular mechanisms of how CTCF binding influences CPD formation.
Article
Endocrinology & Metabolism
Shengjie Zeng, Liuxun Chen, Xvdong Liu, Haibin Tang, Hao Wu, Chuan Liu
Summary: In this study, it was found that the Wnt signaling pathway is downregulated in spermatogonia in non-obstructive azoospermia (NOA), and three transcription factors (CTCF, AR, and ARNTL) are implicated in this dysfunctional Wnt signaling. These findings provide new insights into the mechanisms of NOA and offer potential therapeutic targets for NOA patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Mingsen Li, Huaxing Huang, Lingyu Li, Chenxi He, Liqiong Zhu, Huizhen Guo, Li Wang, Jiafeng Liu, Siqi Wu, Jingxin Liu, Tao Xu, Zhen Mao, Nan Cao, Kang Zhang, Fei Lan, Junjun Ding, Jin Yuan, Yizhi Liu, Hong Ouyang
Summary: This study reveals that adult stem cell identity and homeostasis are regulated by lineage-restricted transcriptional regulatory networks, with an emphasis on the critical roles of RUNX1 and SMAD3 in maintaining corneal epithelial identity and homeostasis. Furthermore, the cooperation of RUNX1, PAX6, and SMAD3 establishes a core transcription regulatory circuitry for governing lineage-specific cis-regulatory atlas in limbal stem/progenitor cells.
NATURE COMMUNICATIONS
(2021)
Article
Ophthalmology
Xin Zuo, Hao Zeng, Bowen Wang, Xue Yang, Dalian He, Li Wang, Hong Ouyang, Jin Yuan
Summary: The study reveals that excessive oxidative stress-induced ferroptosis is involved in the pathogenesis of dry eye disease (DED). The expression of AKR1C1 is regulated by NRF2 and plays a protective role in reducing ferroptosis-induced cell damage and inflammation in corneal epithelial cells.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Cell Biology
Jian-Feng Xiang, Victor G. Corces
Summary: This review highlights the recent advancements in understanding nuclear architecture, with a focus on CTCF function, to gain a mechanistic understanding of the principles by which chromatin is folded in the eukaryotic nucleus.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2021)
Article
Medicine, General & Internal
Zhaohua Cai, Zi Wang, Ruosen Yuan, Mingli Cui, Yimin Lao, Ying Wang, Peng Nie, Linghong Shen, Jing Yi, Ben He
Summary: SENP3 is highly expressed in vascular smooth muscle cells during vascular remodeling, promoting cell proliferation and migration through interaction with beta-catenin and inhibition of its degradation, suggesting a crucial role as a redox sensor and mediator in vascular remodeling.
Article
Cell Biology
Qian Nie, Huimin Chen, Ming Zou, Ling Wang, Min Hou, Jia-Wen Xiang, Zhongwen Luo, Xiao-Dong Gong, Jia-Ling Fu, Yan Wang, Shu-Yu Zheng, Yuan Xiao, Yu-Wen Gan, Qian Gao, Yue-Yue Bai, Jing-Miao Wang, Lan Zhang, Xiang-Cheng Tang, Xuebin Hu, Lili Gong, Yizhi Liu, David Wan-Cheng Li
Summary: The protein PIAS1 plays a key role in regulating stress-induced lens pathogenesis by enhancing p53 transcription activity to promote Bax upregulation, thus facilitating stress-induced apoptosis in lens epithelial cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)