Article
Immunology
Ricardo S. Paiva, Camila Ramos, Sara R. Azenha, Carolina Alves, Afonso P. Basto, Luis Graca, Vera C. Martins
Summary: This study demonstrated the involvement of Ppic in differentiation of B lymphocytes and iNKT cells using a Ppic-deficient mouse model, while showing its dispensability for other immune cell lineages in vivo under steady state conditions.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Biophysics
Silvia Napolitano, Aditya Pokharna, Rudi Glockshuber, Alvar D. Gossert
Summary: The study reveals that certain proline residues in Trx can accelerate the rate-limiting trans-to-cis proline isomerization during protein folding, with trans-P68 playing a critical role in enhancing Trx folding kinetics.
BIOPHYSICAL JOURNAL
(2021)
Article
Multidisciplinary Sciences
Robert Hromas, Gayathri Srinivasan, Ming Yang, Aruna Jaiswal, Taylor A. Totterdale, Linda Phillips, Austin Kirby, Nazli Khodayari, Mark Brantley, Elizabeth A. Williamson, Kimi Y. Kong
Summary: BRCA1-def cancer cells survive their increased unfolded protein burden by targeting the degradation of PERK and IRE1, which are stress sensors, and this finding can be used to develop new therapeutic strategies for treating these cancers.
Review
Biology
Deepti Gurung, Jacob A. Danielson, Afsara Tasnim, Jian-Ting Zhang, Yue Zou, Jing-Yuan Liu
Summary: Proline isomerization is an important post-translational modification that affects protein folding, conformation, and biological pathways. However, its importance and prevalence as a regulation mechanism in biological systems have not been fully understood. In this review, we aim to connect various aspects of proline isomerization, including its chemistry, historical discovery, biological function, and related diseases, to fill the gaps and raise awareness. Additionally, therapeutic opportunities and urgent needs related to proline isomerization are discussed.
Article
Cell Biology
Yingying Zhu, Shuaigao Chen, Weiwei Liu, Fanxing Xu, Jingyu Lu, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: Inhibition of the IGF-1R/YAP signaling pathway is involved in collagen V-induced insulin biosynthesis and secretion in INS-1 cells.
CONNECTIVE TISSUE RESEARCH
(2022)
Article
Toxicology
Xiao-Yu Song, Peng-Cheng Liu, Wei-Wei Liu, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: The study investigated the protective role of Silibinin in ethanol or acetaldehyde-induced apoptosis in human liver cancer cells and liver cells, and found that Silibinin improved cell viability and mitochondrial function, reduced enzyme activities, inhibited apoptosis, and regulated the expression of proteins associated with mitochondrial fission and fusion.
TOXICOLOGY IN VITRO
(2022)
Article
Cell Biology
Stefan Preisendoerfer, Yoshihiro Ishikawa, Elisabeth Hennen, Stephan Winklmeier, Jonas C. Schupp, Larissa Knueppel, Isis E. Fernandez, Leonhard Binzenhofer, Andrew Flatley, Brenda M. Juan-Guardela, Clemens Ruppert, Andreas Guenther, Marion Frankenberger, Rudolf A. Hatz, Nikolaus Kneidinger, Jurgen Behr, Regina Feederle, Aloys Schepers, Anne Hilgendorff, Naftali Kaminski, Edgar Meinl, Hans Peter Baechinger, Oliver Eickelberg, Claudia A. Staab-Weijnitz
Summary: The study reveals that FKBP11 plays an important role in plasma cells, is regulated by XBP1, and sheds light on the important mechanisms involved in antibody production and folding.
Article
Toxicology
Xiao-Yu Song, Peng-Cheng Liu, Wei-Wei Liu, Jia Zhou, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: This study elucidates the protective effects of silibinin on ethanol- or acetaldehyde-induced liver cell damage by modulating ferroptosis in human liver cancer cells and immortalized liver cells. Silibinin resolves oxidative stress and reduces iron levels, rescues ferroptosis induced by ethanol or acetaldehyde, and reverses mitophagy arrest. These findings provide new therapeutic strategies for alcoholic liver injury.
TOXICOLOGY IN VITRO
(2022)
Article
Biochemistry & Molecular Biology
Yanfang Gao, Kai Ma, Yu Kang, Weiwei Liu, Xiaoling Liu, Xinyu Long, Toshihiko Hayashi, Shunji Hattori, Kazunori Mizuno, Hitomi Fujisaki, Takashi Ikejima
Summary: The extracellular matrix (ECM) regulates cell behavior and provides a suitable environment for cell survival. Type I collagen, as one of its major components, is involved in regulating cell migration, proliferation, and differentiation. This study found that type I collagen inhibits adipogenic differentiation and inactivates autophagy by activating the YAP pathway and up-regulating mTOR activity. These findings provide insight into the strategy for dealing with obesity by improving glycolipid metabolism and maintaining glucose and lipid homeostasis in the body.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Multidisciplinary Sciences
Masahiko Terajima, Yuki Taga, Tomoyuki Nakamura, Hou-Fu Guo, Yukako Kayashima, Nobuyo Maeda-Smithies, Kshitij Parag-Sharma, Jeong Seon Kim, Antonio L. Amelio, Kazunori Mizuno, Jonathan M. Kurie, Mitsuo Yamauchi
Summary: This study investigated the role of LH2 in collagen stability, organization, and mineralization using LH2-null MC3T3 cells. The results demonstrated that LH2 is involved in the hydroxylation of telopeptidyl Lys residues of collagen and affects collagen solubility, fibril diameter, and mineralization.
SCIENTIFIC REPORTS
(2022)
Article
Behavioral Sciences
Chenkang Wang, Yu Kang, Panwen Liu, Weiwei Liu, Wenhui Chen, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: Excessive physical exercise can result in damage to vital tissues, including the hippocampus, which is important for learning and memory. A combination of dasatinib and quercetin, known as senolytic drugs, selectively kill senescent cells. In this study, rats subjected to excessive swimming training were treated with dasatinib and quercetin, which improved exercise performance and prevented deficits in learning and memory. The drugs reduced senescent cells and apoptosis in the hippocampus, ultimately alleviating the negative effects of overtraining.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Yuki Taga, Thibault Coste, Sara F. Tufa, Douglas R. Keene, Kazunori Mizuno, Elisabeth Tournier-Lasserve, Douglas B. Gould
Summary: Collagens are important proteins in the body, and mutations in genes encoding them can cause connective tissue disorders. Lysyl hydroxylase 3 (LH3) plays a critical role in collagen biosynthesis, and LH3 pathogenic variants may contribute to Gould syndrome.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Panwen Liu, Chenkang Wang, Wenhui Chen, Yu Kang, Weiwei Liu, Zhiyue Qiu, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: Silibinin, a natural flavonoid from Silybum marianum seeds, has neuroprotective effects on sporadic Alzheimer's disease models. It exerts its effects by inhibiting over-activation of N-methyl-D-aspartate receptors (NMDARs).
Article
Biochemistry & Molecular Biology
Can Li, Yuying Zhu, Weiwei Liu, Toshihiko Hayashi, Wendie Xiang, Sijun He, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: UVB irradiation induces skin inflammation and apoptosis. Mitochondrial dynamics play a crucial role in these processes. Inhibiting mitochondrial fission prevents UVB-induced inflammation and apoptosis, while inhibiting mitochondrial fusion increases these processes. Treatment with an antioxidant, NAC, can reduce inflammation and apoptosis, providing a new therapy for UVB skin injury.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Can Li, Yuying Zhu, Weiwei Liu, Wendie Xiang, Sijun He, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: This study focuses on the involvement of mitochondrial disorders in UVB-induced skin damages. UVB irradiation increases the amount of dysfunctional mitochondria in HaCaT cells, leading to decreased ATP production, mitochondrial membrane potential collapse, and apoptosis. The impairment of PINK1/parkin-mediated mitophagy contributes to UVB-induced cell damages by activating cGAS-STING pathway and promoting caspase-3-mediated apoptosis. Restoring mitophagy may be a potential strategy to protect skin from UVB damages.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Nutrition & Dietetics
Kumiko Kuwaba, Masashi Kusubata, Yuki Taga, Hiroshi Igarashi, Koichi Nakazato, Kazunori Mizuno
Summary: This study investigated the effects of dietary collagen peptides (CPs) on post-exercise physical condition and fitness in healthy middle-aged adults unfamiliar with exercise. The results showed that dietary CPs can alleviate muscle soreness and fatigue, and improve muscle strength.
JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Arkadiusz Bonna, Douglas B. Gould, Richard W. Farndale
Summary: Mutations in the FKBP14 gene result in kyphoscoliotic Ehlers-Danlos Syndrome (EDS), and FKBP22 selectively binds to collagens III, IV, VI, and X. The binding distribution of FKBP22 along the collagen helix and its correlation with positive peptide charge have been observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sergei P. Boudko, Vadim K. Pedchenko, Elena N. Pokidysheva, Alena M. Budko, Rachel Baugh, Patrick Toby Coates, Aaron L. Fidler, Heather M. Hudson, Sergey V. Ivanov, Carl Luer, Tetyana Pedchenko, Robert L. Preston, Mohamed Rafi, Roberto Vanacore, Gautam Bhave, Julie K. Hudson, Billy G. Hudson
Summary: Collagen IV scaffold is an innovation that enables the assembly of basement membranes attached to polarized cells. Chloride ions play a crucial role in scaffold assembly. Analysis of hexamers from 34 animal species revealed that solution Cl- stabilizes the structure of hexamers.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)