Article
Environmental Sciences
Cristina del Alamo, Angela Vazquez-Calvo, Antonio Alcami, Juan Sanchez-Garcia-Casarrubios, Jose Luis Perez-Diaz
Summary: The experience of the COVID-19 pandemic has highlighted the importance of preventing the spread of biological agents in the air and on surfaces. This study investigates the effectiveness of the COUNTERFOG (R) technology in disinfecting viral-contaminated surfaces and measures the required disinfection time. The results show that COUNTERFOG (R) can rapidly and efficiently eliminate viruses using sodium hypochlorite solution, with the concentration and exposure time playing a critical role in disinfection efficacy.
FOOD AND ENVIRONMENTAL VIROLOGY
(2022)
Editorial Material
Multidisciplinary Sciences
Ana Teles, Flavia Viana
Summary: Ana Teles and Flavia Viana took the opportunity to communicate their passion for science to migrant children who shared their first language.
Editorial Material
Multidisciplinary Sciences
Nicholas P. Holmes
Summary: The scientific system must incentivize honesty in admitting mistakes in order to accelerate progress.
Article
Computer Science, Cybernetics
Fayaz Ali, Qingyu Zhang, Muhammad Zubair Tauni, Khuram Shahzad
Summary: This study examines the impact of social interaction anxiety on compulsive chat with a social chatbot named Xiaoice, while exploring fear of negative evaluation (FONE) and fear of rejection (FOR) as mediators in this relationship. The authors find that social interaction anxiety increases compulsive chat with Xiaoice both directly and indirectly through FONE and FOR. The mediating effect of FONE operates through FOR, establishing a serial link between social interaction anxiety and compulsive chat with a social chatbot. Frustration about unavailability (FAU) also strengthens the relationship between FOR and compulsive chat with a social chatbot.
INTERNATIONAL JOURNAL OF HUMAN-COMPUTER INTERACTION
(2023)
Article
Information Science & Library Science
Karima Kourtit, Peter Nijkamp, John Osth
Summary: This paper introduces the concept of "city-love" analysis for evidence-based assessment of the attractiveness features of cities or urban neighborhoods. The city-love production function allows for quantitatively uncovering important determinants of citizens' love for their local environment.
INTERNATIONAL JOURNAL OF INFORMATION MANAGEMENT
(2021)
Review
Microbiology
Yousef Nami, Nazila Imeni, Bahman Panahi
Summary: Phages play a key role in microbial community structure and human health, and machine learning methods have been applied to advance phage research in automated curation, identification, classification, and lifecycle prediction.
Article
Multidisciplinary Sciences
Diana P. Pires, Rodrigo Monteiro, Dalila Mil-Homens, Arsenio Fialho, Timothy K. Lu, Joana Azeredo
Summary: In an era where bacteriophages are considered as a promising therapeutic approach for dealing with antibiotic resistance, genetic modifications to enhance antibacterial properties present challenges due to limited DNA encapsulation capacity. By knocking out up to 48% of genes encoding hypothetical proteins from the genome of a newly isolated Pseudomonas aeruginosa phage, synthetic phages with smaller genomes were designed and assembled for the first time. Both in vitro and in vivo studies demonstrated that the knock-outs in phage genomes did not compromise antibacterial efficacy, suggesting that this strategy could facilitate the introduction of other genes of interest for future treatment of P. aeruginosa infections.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Ming Yan, Akbar Adjie Pratama, Sripoorna Somasundaram, Zongjun Li, Yu Jiang, Matthew B. Sullivan, Zhongtang Yu
Summary: By mining published rumen metagenomes, we constructed a global rumen virome database (RVD) and analyzed its diversity, virus-host linkages, and potential roles in affecting rumen functions. RVD, containing 397,180 species-level viral operational taxonomic units (vOTUs), significantly improves the detection rate of rumen viruses compared with other databases. The rumen virome is predicted to infect core members of the rumen microbiome, carry diverse auxiliary metabolic genes, and likely impact the rumen ecosystem.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Jett Liu, Alexander L. Jaffe, Linxing Chen, Batbileg Bor, Jillian F. Banfield
Summary: This study analyzed the CRISPR-Cas systems in Gracilibacteria, Absconditabacteria, and Saccharibacteria, and predicted the phages that infect these bacteria. Some phages were found to infect both standard-coded and alternatively coded CPR bacteria. Additionally, spacers encoded in Actinobacteria were predicted to target some phages of Saccharibacteria.
Article
Microbiology
Taylor Morrisette, Katherine L. Lev, Gregory S. Canfield, Breck A. Duerkop, Razieh Kebriaei, Kyle C. Stamper, Dana Holger, Susan M. Lehman, Smaranda Willcox, Cesar A. Arias, Michael J. Rybak
Summary: Enterococcus faecium is an important multidrug-resistant pathogen. The use of bacteriophage cocktails as a complement to antibiotic therapy has been proposed. This study selected two phages (113 and 9184) with the broadest host ranges for further experiments and found that the combination of Daptomycin and the phage cocktail showed bactericidal activity and prevented phage resistance against daptomycin-nonsusceptible E. faecium.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biology
Pia J. Schucht, Nicole Klein, Markus Lambertz
Summary: A study on the reliability of histology-based skeletochronology in a variety of extant tetrapods revealed significant issues with conflicting age estimates between different approaches. The study highlights the need for further research on the methodological side of skeletochronology.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Microbiology
Chinmay V. Tikhe, George Dimopoulos
Summary: The microbiota of mosquitoes plays a significant role in their biology, impacting processes from larval development to adulthood. By manipulating the microbiota during the larval stage, it is possible to alter mosquito life history traits. Bacteriophages can be used as tools to modulate the mosquito microbiota, providing a potential mechanism for mosquito control.
Article
Biochemistry & Molecular Biology
Katharina G. Wandera, Omer S. Alkhnbashi, Harris V. I. Bassett, Alexander Mitrofanov, Sven Hauns, Anzhela Migur, Rolf Backofen, Chase L. Beisel
Summary: A deep learning algorithm was used to identify a new anti-CRISPR protein targeting type VI-B CRISPR-Cas systems, expanding the diversity of known Acrs. The identified Acr, named AcrVIB1, was found to effectively inhibit Cas13b nucleases and provides insights into controlling CRISPR technologies.
Article
Biochemistry & Molecular Biology
Zoe Netter, Caroline M. Boyd, Tania Silvas, Kimberley D. Seed
Summary: Bacteria face constant threat of predation by bacterial viruses, and one particular bacterial virus satellite, PLE, has developed a specific defense mechanism against lytic phage ICP1. This defense involves molecular parasitism where PLE hijacks ICP1 processes. Through encoding a novel DNA binding protein, CapR, PLE is able to repress ICP1's capsid morphogenesis operon without hindering the overall ICP1 lifecycle. This delicate balance of satellite-mediated inhibition allows PLE to block phage production while successfully mobilizing in a phage-derived particle, showing a unique aspect of bacteriophage interactions.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Abdulkerim Karaynir, Buelent Bozdogan, Hanife Salih Dogan
Summary: This study successfully obtained active bacteriophage by transforming environmental DNA extracted from hospital wastewater into Escherichia coli using chemical transformation and electroporation. The phage, named ADUt, showed polyvalent activity against limited strains of Shigella and Escherichia genera. The genome of ADUt was sequenced and analyzed, revealing its classification and genetic characteristics.
Article
Biochemistry & Molecular Biology
Alicia del Prado, Jose M. Lazaro, Elisa Longas, Laurentino Villar, Miguel de Vega, Margarita Salas
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Nadine Fornelos, Matej Butala, Vesna Hodnik, Gregor Anderluh, Jaana K. Bamford, Margarita Salas
NUCLEIC ACIDS RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Isabel Holguera, Daniel Munoz-Espin, Margarita Salas
NUCLEIC ACIDS RESEARCH
(2015)
Article
Multidisciplinary Sciences
Monica Berjon-Otero, Laurentino Villar, Miguel de Vega, Margarita Salas, Modesto Redrejo-Rodriguez
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Virology
Laura Mojardin, Margarita Salas
JOURNAL OF VIROLOGY
(2016)
Article
Biochemistry & Molecular Biology
Monica Berjon-Otero, Laurentino Villar, Margarita Salas, Modesto Redrejo-Rodriguez
NUCLEIC ACIDS RESEARCH
(2016)
Article
Multidisciplinary Sciences
Pablo Gella, Margarita Salas, Mario Mencia
Article
Virology
Monica Berjon-Otero, Ana Lechuga, Jitender Mehla, Peter Uetz, Margarita Salas, Modesto Redrejo-Rodriguez
JOURNAL OF VIROLOGY
(2017)
Article
Cell Biology
Modesto Redrejo-Rodriguez, Carlos D. Ordonez, Monica Berjon-Otero, Juan Moreno-Gonzalez, Cristian Aparicio-Maldonado, Patrick Forterre, Margarita Salas, Mart Krupovic
Article
Biochemistry & Molecular Biology
Nadine Fornelos, Douglas F. Browning, Anja Pavlin, Zdravko Podlesek, Vesna Hodnik, Margarita Salas, Matej Butala
NUCLEIC ACIDS RESEARCH
(2018)
Article
Multidisciplinary Sciences
Alicia del Prado, Elsa Franco-Echevarria, Beatriz Gonzalez, Luis Blanco, Margarita Salas, Miguel de Vega
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Pauline van Nies, Ilja Westerlaken, Duco Blanken, Margarita Salas, Mario Mencia, Christophe Danelon
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Ana Lechuga, Cedric Lood, Monica Berjon-Otero, Alicia del Prado, Jeroen Wagemans, Vera van Noort, Rob Lavigne, Margarita Salas, Modesto Redrejo-Rodriguez
Summary: By integrating the traditional yeast two-hybrid system and high-throughput sequencing, researchers constructed a protein-protein interaction network for the Bam35-Bacillus thuringiensis model and identified 182 high-confidence virus-host interactions. Within these interactions, host metabolism proteins and peptidases were particularly enriched, distinguishing this host-phage system from others reported in the literature.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Meeting Abstract
Biophysics
J. A. Morin, F. J. Cao, M. Salas, B. Ibarra
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2015)
Review
Biochemistry & Molecular Biology
Margarita Salas, Isabel Holguera, Modesto Redrejo-Rodriguez, Miguel de Vega
FRONTIERS IN MOLECULAR BIOSCIENCES
(2016)