Article
Biology
Lise M. Sjogaard-Frich, Andreas Prestel, Emilie S. Pedersen, Marc Severin, Kristian Kolby Kristensen, Johan G. Olsen, Birthe B. Kragelund, Stine Falsig Pedersen
Summary: Through various biophysical techniques, this study reveals the complex interaction mechanism between CaM and NHE1, identifying multiple states and structures of the NHE1:CaM complex that can be modulated by stoichiometries, calcium concentrations, and phosphorylation. Experimental results suggest that CaM may enhance NHE1 regulation and indicate that this structural diversity is relevant to other CaM complexes as well.
Article
Multidisciplinary Sciences
Yi Liu, Efren Reyes, David Castillo-Azofeifa, Ophir D. Klein, Todd Nystul, Diane L. Barber
Summary: Liu et al. find that intracellular pH dynamics regulate the secretory cell fate decision of intestinal stem cells. They observe an intracellular pH gradient in mouse small intestinal crypts, with the lowest pH in crypt stem cells and increasing along the crypt column. Disrupting this gradient inhibits crypt budding and Paneth cell differentiation, which can be rescued by exogenous WNT. They demonstrate that intracellular pH dynamics acts downstream of ATOH1, with increased pH promoting differentiation toward the secretory lineage. These findings establish a role for intracellular pH dynamics in cell fate decisions within an adult stem cell lineage.
NATURE COMMUNICATIONS
(2023)
Article
Cardiac & Cardiovascular Systems
Yu Jin Chung, Kyung Chan Park, Sergiy Tokar, Thomas R. Eykyn, William Fuller, Davor Pavlovic, Pawel Swietach, Michael J. Shattock
Summary: Research indicates that cardiac NHE1 activity is not inhibited by EMPA (or other SGLT2i's) and EMPA has no effect on [Na+] over a wide range of concentrations, including the therapeutic dose.
CARDIOVASCULAR RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yuanye Tian, Qi Shao, Yi Tang, Xinzhao Li, Xin Qi, Runyang Jiang, Yi Liang, Feiwu Kang
Summary: HIF-1 alpha plays a key role in osteoclastogenesis and bone resorption during fracture healing, and influences bone healing through CT-1 secretion. These findings are important for understanding the pathogenesis of bone diseases.
Review
Chemistry, Medicinal
Huiting Xia, Aqeela Zahra, Meng Jia, Qun Wang, Yunfu Wang, Susan L. Campbell, Jianping Wu
Summary: In this review, the potential role of NHE1 in cardiac hypertrophy and heart failure is discussed, along with an investigation into the underlying molecular mechanisms.
Article
Medicine, Research & Experimental
Zheng Zhang, Minghui Wang, Youli Zheng, Yanmei Dai, Jiashu Chou, Xiaowei Bian, Pengcheng Wang, Changyi Li, Jing Shen
Summary: This study found that miR-223 is upregulated in periodontitis and downregulated during the osteogenesis of PDL-derived cells. Overexpression of miR-223 inhibits osteogenic differentiation of PDL-derived cells, potentially by targeting the growth factor receptor genes FGFR2 and TGFβR2.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Xiuju Li, Benjamin Buckley, Konstantin Stoletov, Yang Jing, Marie Ranson, John D. Lewis, Mike Kelso, Larry Fliegel
Summary: This study examined the role of NHE1 and uPA in prostate cancer cell migration and colony formation, finding that only uPA activity played a critical role in cell migration and colony formation, while the effect of NHE1 was minor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Christine Skerka, Gabriele Pradel, Luke D. Halder, Peter F. Zipfel, Svante L. H. Zipfel, Olaf Strauss
Summary: FHR-1, a member of the factor H protein family, plays a crucial role in regulating innate immune complement reactions. Recent studies have shown its involvement in various diseases and potential role in inflammation induced by necrosis. This review provides insights into the role of FHR-1 in pathology and identifies it as a potential therapeutic target for inflammatory diseases.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Microbiology
Joao Paulo Ferreira Rodrigues, Leonardo Loch, Thiago Souza Onofre, Nobuko Yoshida
Summary: The involvement of NHE1 in the internalization of Trypanosoma cruzi metacyclic trypomastigotes was studied. NHE1 plays a role in maintaining the activation status of diverse protein kinases and preventing inappropriate F-actin arrangement, thus affecting lysosome distribution.
Article
Biochemistry & Molecular Biology
Xu Liu, Yue Qiu, Ning Huang, Yan-Hua Liu, Huan-Huan Wang, Ya-Nan Yu, Yu-Ting Song, Guang-Rui Wan, Shuang-Xi Wang, Peng Li, Ya-Ling Yin
Summary: This study found that CT could alleviate DOX-induced cardiotoxicity in rats by regulating oxidative stress and apoptosis-related factors, as well as inhibiting NHE1 upregulation. The protective effects of CT were affected by the concurrent administration of LiCl.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Medicine, Research & Experimental
Laween Uthman, Xiaoling Li, Antonius Baartscheer, Cees A. Schumacher, Patricia Baumgart, Jeroen Hermanides, Benedikt Preckel, Markus W. Hollmann, Ruben Coronel, Coert J. Zuurbier, Nina C. Weber
Summary: Inflammation leading to oxidative stress in endothelial cells is linked to heart failure development. The study suggests that TNF-alpha can activate NHE to raise [Na+](c), triggering ROS production, and EMPA can reduce inflammation-induced ROS by inhibiting NHE activity.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Engineering, Biomedical
Clement Bechtler, Sophia Koutsogiannaki, Ekaterina Umnyakova, Amal Hamid, Avneesh Gautam, Yiannis Sarigiannis, Richard B. Pouw, Christina Lamers, Said Rabbani, Christoph Q. Schmidt, John D. Lambris, Daniel Ricklin
Summary: The use of biomaterials in modern medicine has advanced drug delivery strategies and reduced morbidity and mortality. However, immune reactions remain a serious complication.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Gen L. Takei, Yuhei Ogura, Yoshihiro Ujihara, Fubito Toyama, Keitaro Hayashi, Tomoe Fujita
Summary: Previous studies have shown that extracellular Na+ suppresses hamster sperm hyperactivation by reducing intracellular Ca2+ levels, and this suppressive effect can be canceled by specific inhibitors of Na+/Ca2+-exchanger (NCX). However, direct evidence of the presence and functionality of NCX in hamster spermatozoa is lacking. This study demonstrates the presence of functional NCX1 in hamster spermatozoa and its downregulation upon capacitation to trigger hyperactivation, providing novel insights into the regulation of sperm hyperactivation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Xiuju Li, Larry Fliegel
Summary: In breast cancer, pH regulatory proteins and the tumor microenvironment play a crucial role in cancer cell metastasis. This study found that the acid-extruding protein NHE1 has an important role in the migration and invasion of basal-like triple-negative breast cancer cells.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Pathology
Yoshiyasu Fukusumi, Hidenori Yasuda, Ying Zhang, Hiroshi Kawachi
Summary: This study investigated the role of ephrin-B1 and its associated molecules in the slit diaphragm of kidney glomerular podocytes. It was found that the interactions between ephrin-B1, NHERF2, and other molecules formed a critical signaling axis in podocytes, with phosphorylation and dephosphorylation playing key regulatory roles.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Endocrinology & Metabolism
Alvaro Gonzalez, Cira Garcia de Durango, Veronica Alonso, Beatriz Bravo, Arancha Rodriguez de Gortazar, Alan Wells, Jeronimo Forteza, Fernando Vidal-Vanaclocha
Article
Hematology
Gyun Jee Song, Stacey Barrick, Kristen L. Leslie, Philip M. Bauer, Veronica Alonso, Peter A. Friedman, Nathalie M. Fiaschi-Taesch, Alessandro Bisello
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2012)
Article
Endocrinology & Metabolism
Veronica Alonso, Juan A. Ardura, Bin Wang, W. Bruce Sneddon, Peter A. Friedman
JOURNAL OF BONE AND MINERAL RESEARCH
(2011)
Article
Endocrinology & Metabolism
Veronica Alonso, Clara E. Magyar, Bin Wang, Alessandro Bisello, Peter A. Friedman
JOURNAL OF BONE AND MINERAL RESEARCH
(2011)
Article
Cell Biology
Veronica Alonso, Arancha R. De Gortazar, Juan A. Ardura, Irene Andrade-Zapata, M. Victoria Alvarez-Arroyo, Pedro Esbrit
JOURNAL OF CELLULAR PHYSIOLOGY
(2008)
Article
Medicine, Research & Experimental
V. Alonso, F. C. Perez-Martinez, F. J. Calahorra, P. Esbrit
Article
Oncology
Juan A. Ardura, Irene Gutierrez-Rojas, Luis Alvarez-Carrion, M. Rosario Rodriguez-Ramos, Jose M. Pozuelo, Veronica Alonso
Review
Endocrinology & Metabolism
Juan A. Ardura, Sergio Portal-Nunez, Veronica Alonso, Beatriz Bravo, Arancha R. Gortazar
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2019)
Review
Pharmacology & Pharmacy
Juan A. Ardura, Gorjana Rackov, Elena Izquierdo, Veronica Alonso, Arancha R. Gortazar, Maria M. Escribese
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Cell Biology
Eduardo Martin-Guerrero, Irene Tirado-Cabrera, Irene Buendia, Veronica Alonso, Arancha R. Gortazar, Juan A. Ardura
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Review
Oncology
Juan A. Ardura, Luis Alvarez-Carrion, Irene Gutierrez-Rojas, Veronica Alonso
Article
Oncology
Juan A. Ardura, Luis Alvarez-Carrion, Irene Gutierrez-Rojas, Peter A. Friedman, Arancha R. Gortazar, Veronica Alonso
ENDOCRINE-RELATED CANCER
(2020)
Article
Oncology
Luis Alvarez-Carrion, Irene Gutierrez-Rojas, Maria Rosario Rodriguez-Ramos, Juan A. Ardura, Veronica Alonso
Summary: Prostate cancer is a leading cause of death in men worldwide. A study found that the matrix protein MINDIN promotes prostate cancer progression by downregulating the adaptor protein NHERF-1, leading to increased tumor cell migration and proliferation. This suggests that targeting MINDIN and NHERF-1 could be potential strategies for future prostate cancer therapies.
Article
Biochemistry & Molecular Biology
Juan A. Ardura, Veronica Alonso, Pedro Esbrit, Peter A. Friedman
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)