Article
Multidisciplinary Sciences
Audun Kvalvaag, Salvatore Valvo, Pablo F. Cespedes, David G. Saliba, Elke Kurz, Kseniya Korobchevskaya, Michael L. Dustin
Summary: Ligation of TCR to pMHC induces TCR ubiquitination and either internalization by T cell or release in extracellular vesicles. Clathrin-mediated ectocytosis facilitates the release of TCR, while clathrin-mediated trans-endocytosis enables the uptake of pMHC-TCR conjugates from the APC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Alexander Johnson, Dana A. Dahhan, Nataliia Gnyliukh, Walter A. Kaufmann, Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, Monika Hrtyan, Jie Wang, Juan Aguilera-Servin, Daniel Van Damme, Emmanuel Beaurepaire, Martin Loose, Sebastian Y. Bednarek, Jiri Friml
Summary: This study identified the TPLATE complex as a key component in the unique plant endocytosis mechanism, playing a crucial role in mediating membrane bending against high-turgor pressure in plant cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Tania Lopez-Hernandez, Koh-Ichiro Takenaka, Yasunori Mori, Pornparn Kongpracha, Shushi Nagamori, Volker Haucke, Shigeo Takamori
Summary: This study found that the endocytosis of synaptic vesicles in mouse hippocampal neurons at physiological temperature is independent of clathrin, while the endocytic retrieval of certain synaptic vesicle proteins, including vesicular transporters for glutamate and GABA, relies on sorting by the clathrin adaptor AP-2.
Article
Biochemistry & Molecular Biology
Wei Siao, Peng Wang, Xiuyang Zhao, Lam Dai Vu, Ive De Smet, Eugenia Russinova
Summary: Phosphorylation of the small subunit of ADAPTOR PROTEIN-2 by ADAPTOR-ASSOCIATED PROTEIN KINASE1 has an impact on Arabidopsis root tropic growth. AAK1 is involved in clathrin-mediated endocytosis regulation in mammals by phosphorylating the μ2 subunit of the AP-2 complex. Previous research found an association between AAK1 and AP-2 in Arabidopsis, but its function was unclear. In this study, genetic analysis revealed that both aak1 and ap2m mutants in Arabidopsis exhibit altered root tropic growth, including impaired touch and gravity responses. Phosphorylation of AP2M on Thr-163 by AAK1 fine-tunes endocytosis in the Arabidopsis root to control tropic growth.
Review
Cell Biology
Henri-Francois Renard, Emmanuel Boucrot
Summary: Endocytosis is a crucial mechanism for cellular uptake, also utilized by viruses, toxins, and bacteria for cell invasion. Besides the canonical clathrin-mediated pathway, there are various clathrin-independent mechanisms for internalizing specific cargoes and supporting cellular functions.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Cell Biology
Shikha T. Ramesh, Kolaparamba V. Navyasree, Sneha Sah, Anjitha B. Ashok, Nishada Qathoon, Suryasikha Mohanty, Rajeeb K. Swain, Perunthottathu K. Umasankar
Summary: This study uncovers the role of an uncharacterized kinase BMP2K in AP-2 phosphorylation, demonstrating that BMP2K interacts with AP-2 via its C-terminus, and this interaction is crucial for CCP localization and function. The decline of endogenous BMP2K levels upon AP-2 impairment suggests a stabilizing relationship, and functional inactivation of BMP2K in zebrafish embryos leads to gastrulation phenotypes similar to AP-2 loss-of-function, highlighting the physiological relevance of BMP2K in vertebrates.
Article
Biochemistry & Molecular Biology
Luyao Wang, Pei Wen, Joyce van de Leemput, Zhanzheng Zhao, Zhe Han
Summary: By using Drosophila nephrocytes as a genetic screen platform, this study identified key components of the SD protein endocytosis and recycling pathway, highlighting their importance in maintaining the filtration structure. The findings demonstrate that the components in this pathway are highly conserved and play a role in the endocytosis and recycling of SD proteins in human kidney, suggesting mutations in these genes could lead to renal diseases.
CELL AND BIOSCIENCE
(2021)
Article
Virology
Zhaoyang Ji, Hui Dong, Ruixue Jiao, Xiaoyuan Zhu, Hongyan Shi, Jianfei Chen, Da Shi, Jianbo Liu, Zhaoyang Jing, Jialin Zhang, Xiaobo Wang, Dandan Ye, Jiyu Zhang, Xin Zhang, Li Feng
Summary: The coronavirus membrane protein plays a central role in the viral life cycle, and this study reveals a new role for the M protein in the initial stages of TGEV replication. The interaction between HSC70 and M protein mediates the internalization of TGEV through clathrin-mediated endocytosis. These findings provide new insights into the life cycle of coronaviruses and may aid in the development of therapeutic agents for porcine diarrhea.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaokang Zhang, Chao Wang, Linlu Qi, Shiwei Wang, Yitong Chen, Zhiwei Kong, Saijie Li, Xin Zhang, Zhengguang Zhang, Junfeng Liu, Dongli Wang
Summary: Clathrin-mediated endocytosis (CME) is crucial for physiological processes in eukaryotic cells. In fungi, the Pan1/End3/Sla1 complex regulates the transition between early and late stages of CME. This study uncovers the detailed interaction mechanism between MoEnd3 and MoSla1 in the pathogenic fungus Magnaporthe oryzae, revealing the synergistic effect of EH domains in improving affinity and specificity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Peripheral Vascular Disease
Shu-Song Du, Xue Sun, Jie Cen, Jun-Xiu Shi, Ming-Xin An, Wei-Dong Zhao
Summary: This study investigated the expression profiles of dynamin isoforms and their roles in endocytosis in brain endothelial cells. Dyn2 was found to be the highest expressed isoform, and it was necessary for transferrin uptake and played a role in the late stage of vesicle fission during endocytosis. The results suggested that Dyn2 mediates clathrin-dependent endocytosis for amyloid-beta internalization from basolateral to apical side into brain endothelial cells, while Dyn1 and Dyn3 did not have the same role.
MICROVASCULAR RESEARCH
(2021)
Article
Plant Sciences
Deepanksha Arora, Daniel Van Damme
Summary: Endomembrane trafficking allows proteins and lipids to flow between different endomembrane compartments in eukaryotic cells, largely through vesicle-mediated transport. This process involves packaging transmembrane proteins into vesicles, followed by vesicle scission, uncoating, and fusion at the target membrane. Three major trafficking pathways (anterograde, retrograde, endocytic) operate inside cells, each involving specific machinery and coat proteins for packaging and delivery along the pathways.
Article
Virology
Colleen L. Mayberry, Michael P. Wilczek, Tristan M. Fong, Sarah L. Nichols, Melissa S. Maginnis
Summary: JC polyomavirus (JCPyV) infects the majority of the population, causing a lifelong asymptomatic infection in the kidney, but can lead to severe consequences in immunocompromised individuals. Research has shown that the interaction between 5-HT2R receptors and other cellular proteins is crucial for JCPyV internalization and infection.
JOURNAL OF VIROLOGY
(2021)
Editorial Material
Cell Biology
Sisi Yang, Daniel A. Colon-Ramos
Summary: ATG-9 is involved in synaptic autophagy and exhibits activity-dependent exo-endocytosis at synapses. It is transported from the trans-Golgi network to synapses via the AP-3 complex in C. elegans. Disruption of the endocytosis pathway leads to abnormal ATG-9 accumulation and defects in activity-induced synaptic autophagy.
Article
Cell Biology
Andrew K. Lamb, Andres N. Fernandez, Olve B. Peersen, Santiago M. Di Pietro
Summary: The Tda2-Aim21 complex plays a crucial role in regulating actin assembly during endocytosis, with Tda2 acting as a dimerization engine to bring together two Aim21 molecules using a novel binding surface. Additionally, a CP interacting motif was found in Aim21, expanding its function to a fundamental cellular pathway and showing the existence of such motif outside mammalian cells.
MOLECULAR BIOLOGY OF THE CELL
(2021)