Article
Microbiology
Nujud Almuzaini, Madison Moore, Marjorie Robert-Guroff, Michael A. Thomas
Summary: The study reveals that the viral oncogene E4orf3 plays a role in promoting DNA > 4n in adenovirus-infected cells and may be linked to its oncogenic potential.
MICROBIOLOGY SPECTRUM
(2022)
Article
Cell Biology
Sheema Almozyan, James Coulton, Roya Babaei-Jadidi, Abdolrahman S. Nateri
Summary: Recent studies have indicated that FLYWCH1 may play a critical role in facilitating the recruitment of DNA-damage response proteins in the context of DNA damage and repair.
Article
Biochemistry & Molecular Biology
Jinyi Zhou, Lili Gu, Yingying Shi, Ting Huang, Xirui Fan, Xiaowen Bi, Shuai Lu, Juanjuan Liang, Lan Luo, Peng Cao, Zhimin Yin
Summary: The study showed that GSTpi can protect cells from cell death during DNA damage via Ser184 phosphorylation, enhance NBS1 nuclear translocation to activate the ATM-Chk2-p53 signaling pathway, block the cell cycle in the G2/M phase to allow more time for DNA damage repair.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Oncology
Adel Alblihy, Ahmed Shoqafi, Michael S. Toss, Mashael Algethami, Anna E. Harris, Jennie N. Jeyapalan, Tarek Abdel-Fatah, Juliette Servante, Stephen Y. T. Chan, Andrew Green, Nigel P. Mongan, Emad A. Rakha, Srinivasan Madhusudan
Summary: The MRN complex is critical for genomic stability and its low expression in breast cancer is associated with aggressive histopathological phenotypes and poor survival outcomes. Low nuclear RAD50 transcripts, as well as overexpression of hsa-miR-494 and hsa-miR-99b microRNAs, are correlated with adverse clinical outcomes, indicating that MRN status could be a useful tool for stratifying tumors for precision medicine strategies.
Article
Biochemistry & Molecular Biology
Ya Wang, Yuanbing Yao, Qunhui Wei, Shichao Long, Yuqiao Chen, Jinru Xie, Rong Tan, Wei Jiang, Qian Zhang, Dongbo Wu, Shuai Xiao, Fengyi Wan, Kai Fu
Summary: TRIM24, an oncogene overexpressed in cancers, has been identified as a novel signaling molecule in response to DNA double-strand breaks (DSBs). It promotes the recruitment of the MRN complex and activation of downstream signaling. Depletion of TRIM24 enhances sensitivity to cancer therapy and inhibits tumor growth. This study highlights the potential of TRIM24 as a therapeutic target for tumor treatment.
Article
Biology
Iraia Garcia-Santisteban, Alba Llopis, Lenno Krenning, Jon Vallejo-Rodriguez, Bram van den Broek, Ana M. Zubiaga, Rene H. Medema
Summary: ATM becomes dispensable for G1 checkpoint maintenance as early as 1 hour after DSB induction, while CHK2 kinase activity is necessary to maintain G1 arrest independently of other proteins, suggesting that the G1 arrest is sustained in a lesion-independent manner.
Review
Immunology
Thomas J. Weitering, Sanami Takada, Corry M. R. Weemaes, Pauline A. van Schouwenburg, Mirjam van der Burg
Summary: ATM is often considered the key regulator of the DNA double-stranded break response, yet patients with ataxia telangiectasia surprisingly exhibit only mild immunodeficiency compared to other DSB repair syndromes. Despite its numerous functions, ATM is not essential for acquiring sufficient levels of immunological diversity to prevent severe infections, although there is a notable antibody deficiency in ataxia telangiectasia patients due to disrupted class switch recombination.
TRENDS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Qin Zhang, Lujie Yang, Han Gao, Xunjie Kuang, He Xiao, Chen Yang, Yi Cheng, Lei Zhang, Xin Guo, Yong Zhong, Mengxia Li
Summary: APE1 is upregulated in cervical tumor tissue and promotes radio-resistance. It activates non-homologous end joining (NHEJ) repair and plays a role in temporal formation and repair of DSBs. APE1 deficiency leads to DSB accumulation at a late phase following oxidative stress.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jozef Ba Tran, Michal Padjasek, Artur Krezel
Summary: The metal binding at protein-protein interfaces is still a field that has not been thoroughly studied. This study uses sequence-structure-stability analysis of human Rad50 protein to identify the structural components that increase the stability of the protein complex. The study also reveals that phosphorylation of the zinc hook domain weakens its stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Xiaochen Xie, Ye Zhang, Zhuo Wang, Shanshan Wang, Xiaoyou Jiang, Hongyan Cui, Tingting Zhou, Zheng He, Hao Feng, Qiqiang Guo, Xiaoyu Song, Liu Cao
Summary: ROS, initially considered pathological, are now recognized as signaling intermediates that promote cellular adaptation to stress through autophagy regulation. ATM protein, activated by ROS, is involved in autophagy regulation, maintaining genome stability and triggering cytoplasmic autophagy as a ROS sensor.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Stavroula Tsaridou, Georgia Velimezi, Frances Willenbrock, Maria Chatzifrangkeskou, Waheba Elsayed, Andreas Panagopoulos, Dimitris Karamitros, Vassilis Gorgoulis, Zoi Lygerou, Vassilis Roukos, Eric O'Neill, Dafni Eleftheria Pefani
Summary: This study identified a novel role for the tumor suppressor RASSF1A at rDNA break sites, providing mechanistic insight into how the DNA damage response is organized in a chromatin context and further evidence for how silencing of the RASSF1A tumor suppressor contributes to genome instability.
Article
Microbiology
Weiyin Xu, Ping Yan, Ziyan Zhou, Jingting Yao, Haochun Pan, Luyao Jiang, Zongyi Bo, Bo Ni, Mingxia Sun, Song Gao, Changchao Huan
Summary: Pseudorabies virus (PRV) infection in swine can cause high morbidity and mortality, leading to significant economic losses. In this study, the role of histone deacetylase 6 (HDAC6) in PRV infection was investigated. The inhibition of HDAC6 significantly decreased PRV replication, while its overexpression promoted PRV replication. PRV infection induced DNA damage response (DDR), and HDAC6 inhibition and knockout decreased DDR, suggesting that HDAC6 may be a crucial factor in promoting PRV replication.
MICROBIOLOGY SPECTRUM
(2023)
Article
Oncology
Jingjing Zhang, Qiong Wu, Lucheng Zhu, Shujun Xie, Linglan Tu, Yuhong Yang, Kan Wu, Yanyan Zhao, Yuqing Wang, Yasi Xu, Xueqin Chen, Shenglin Ma, Shirong Zhang
Summary: SERPINE2 is identified as a regulator of radiosensitivity and the DNA damage response (DDR) in lung cancer, with knockdown improving tumor radiosensitivity by reducing homologous recombination repair, restoring cell cycle checkpoints, and suppressing migration and invasion. Additionally, high SERPINE2 expression correlates with poor prognosis and a high serum concentration predicts a poor response to radiotherapy in lung cancer patients.
Article
Medicine, General & Internal
Ryoko Horigome, Kenya Kamimura, Yusuke Niwa, Kohei Ogawa, Ken-Ichi Mizuno, Koichi Fujisawa, Naoki Yamamoto, Taro Takami, Tomoyuki Sugano, Akira Sakamaki, Hiroteru Kamimura, Masaaki Takamura, Shuji Terai
Summary: The DSS-induced colitis mouse model causes colon shortening and increased disease activity index, while also activating the Atm-Chk2 pathway and inducing cell apoptosis at the cellular level. Knockout mice lacking the Ccndbp1 gene exhibit better protection against DSS-induced colitis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Keita Tomioka, Tatsuo Miyamoto, Silvia Natsuko Akutsu, Hiromi Yanagihara, Kazumasa Fujita, Ekaterina Royba, Hiroshi Tauchi, Takashi Yamamoto, Iemasa Koh, Eiji Hirata, Yoshiki Kudo, Masao Kobayashi, Satoshi Okada, Shinya Matsuura
Summary: Genetic information is protected against genotoxins such as ionizing radiation through DNA double-strand break repair. Studies suggest that variants in DNA repair genes may affect individual differences in chromosomal radiosensitivity. The NBS1 I171V variant was found in a Japanese ovarian cancer patient and may contribute to increased radiosensitivity, as seen in experiments with knock-in HCT116 cells and MEFs.
SCIENTIFIC REPORTS
(2021)