Article
Microbiology
Sheyda Azimi, Jacob Thomas, Sara E. Cleland, Jennifer E. Curtis, Joanna B. Goldberg, Stephen P. Diggle
Summary: This study reveals that changes in O-specific antigen determine the spatial organization of P. aeruginosa cells in cystic fibrosis airways by altering the cell surface hydrophobicity. This finding suggests a role for O-antigen in regulating P. aeruginosa aggregate size and shape in cystic fibrosis airways.
Article
Microbiology
Alexandra J. J. Lee, Georgia Doing, Samuel L. L. Neff, Taylor Reiter, Deborah A. A. Hogan, Casey S. S. Greene
Summary: This article studies the genomic heterogeneity of Pseudomonas aeruginosa strains, compares the expression patterns of core genes and accessory genes between PAO1 and PA14 strains, and finds differences in the expression patterns of some core genes among different strains.
Article
Chemistry, Multidisciplinary
Louis M. M. Mouterde, Julien Couvreur, Maxime M. J. Langlait, Fanny Brunois, Florent Allais
Summary: The study identified a potentially new cyclohexanone monooxygenase (CHMO) sequence from the complete genome of the Pseudomonas aeruginosa strain Pa1242, which was confirmed through bioconversion to obtain a key precursor 2H-HBO. The novel sequence exhibited CHMO activity and achieved high productivity and total conversion rates through optimization of culture conditions.
Article
Biology
Wellington S. Miranda, Q. Cong, A. L. Schaefer, E. K. MacLeod, A. Zimenko, D. Baker, E. P. Greenberg
Summary: Researchers have identified selectivity residues within AHL synthases and receptors using covariation methods, demonstrating their importance in the Las system. This study deepens the understanding of how communication systems evolve and diversify.
Article
Biochemistry & Molecular Biology
Yi Lin, Jiawen Lu, Zilu Yang, Tianming Wang, Hongbiao Li, Shenfei Sha, Zhenmin Liu, Yueliang Zhao, Liping Wang
Summary: In this study, the antimicrobial activity of P. pentosaceus C-2-1 was enhanced by UV mutagenesis, resulting in the production of a mutant strain, C23221, with significantly higher antimicrobial activity. A comparison of the genomes of C23221 and the wild-type strain revealed unique genes and proteins related to bacteriocin biosynthesis in the mutant strain. This study provides a genetic basis for future genetic engineering strategies to increase antimicrobial production in P. pentosaceus C-2-1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Laura J. Dunphy, Kassandra L. Grimes, Nishikant Wase, Glynis L. Kolling, Jason A. Papin
Summary: In a synthetic cystic fibrosis media, distinct species-specific metabolic signatures were revealed for Pseudomonas aeruginosa and Staphylococcus aureus, indicating potential nutrient competition's impact on community dynamics. Both bacteria consumed similar nutrients, suggesting metabolic competition's role in coinfection dynamics. Furthermore, unique metabolites produced by each species may serve as species-specific biomarkers for cystic fibrosis lung infections.
Article
Microbiology
Luke Diorio-Toth, Sidra Irum, Robert F. Potter, Meghan A. Wallace, Muhammad Arslan, Tehmina Munir, Saadia Andleeb, Carey-Ann D. Burnham, Gautam Dantas
Summary: This study used whole-genome sequencing to determine the genotypic mechanisms of beta-lactam resistance in Pseudomonas aeruginosa clinical isolates from three hospitals in Pakistan. The study found that carbapenem resistance was associated with the acquisition of metallo-beta-lactamases or extended-spectrum beta-lactamases and mutations in the porin gene. Furthermore, isolates with multiple resistance mechanisms showed significantly higher levels of imipenem resistance compared to isolates with a single resistance mechanism.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Jan de Witt, Philipp Ernst, Jochem Gaetegens, Stephan Noack, Davina Hiller, Benedikt Wynands, Nick Wierckx
Summary: In recent years, there has been increasing interest in branched short-chain dicarboxylates (BSCD) such as itaconic acid in medicine and biotechnology. They are used as building blocks for plastics and there is a need for microbial upcycling strategies to provide sustainable solutions. BSCDs also exhibit antibacterial properties and immunomodulatory effects, making them potentially valuable in the medical field. This study focused on understanding the microbial metabolism of BSCDs, specifically the degradation pathway in Pseudomonas aeruginosa PAO1 and its transfer to Pseudomonas putida. The study identified new genes and discovered a potential antimicrobial candidate, 2-hydroxyparaconate (2-HP). This study expands the substrate range of P. putida and highlights the potential applications of BSCDs.
METABOLIC ENGINEERING
(2023)
Article
Biology
Ye Li, Shiqi Liu, Yingdan Zhang, Zi Jing Seng, Haoran Xu, Liang Yang, Yilin Wu
Summary: This study reports a unique form of actively regulated long-range directed material transport in structured bacterial communities, mediated by biosurfactants secreted by cells. This mechanism helps to eradicate colonies of a competing species.
Article
Multidisciplinary Sciences
Hiromi Watari, Hiromu Kageyama, Nami Masubuchi, Hiroya Nakajima, Kako Onodera, Pamela J. Focia, Takumi Oshiro, Takashi Matsui, Yoshio Kodera, Tomohisa Ogawa, Takeshi Yokoyama, Makoto Hirayama, Kanji Hori, Douglas M. Freymann, Misa Imai, Norio Komatsu, Marito Araki, Yoshikazu Tanaka, Ryuichi Sakai
Summary: In this study, researchers found that a marine sponge-derived protein can activate the human cytokine receptor MPL, similar to the activation by a molecular chaperone in hematologic malignancies. The sugar-mediated activation of MPL, which is different from the original ligand, suggests that the mode of receptor activation is unpredictably diverse in living organisms.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Hai Yang, Zhen Huang, Jiali Yue, Jianqi Chen, Mingming Yu, Chengming Qu
Summary: Metabolomic analysis showed that the combination therapy of meropenem/amikacin significantly altered relevant metabolites involved in bacterial cell membrane and cell wall synthesis, as well as interfered with multiple metabolic pathways essential for the survival of P. aeruginosa.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Matthew Olsen, Rania Nassar, Abiola Senok, Abdulla Albastaki, John Leggett, Anna Lohning, Mariana Campos, Peter Jones, Simon McKirdy, Lotti Tajouri, Rashed Alghafri
Summary: The study found that mobile phones on a university campus were contaminated with viable microbes, including 173 bacteria, 8 fungi, 8 protists, 53 bacteriophages, 317 virulence factor genes, and 41 distinct antibiotic resistant genes. These results indicate that mobile phones may be responsible for the rise of community acquired infections.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Patrick G. Isenegger, Brian Josephson, Ben Gaunt, Matthew J. Davy, Veronique Gouverneur, Andrew J. Baldwin, Benjamin G. Davis
Summary: This article describes a robust, mild and fast method for the posttranslational, site-directed fluorination of protein sidechains, which can be detected by F-19-based magnetic resonance methods. This chemical editing method is stable, mild, fast and highly efficient, and can be used by non-experts to prepare fluorinated proteins within 24 hours without the need for bespoke biological systems.
Article
Microbiology
Ke Fan, Qiao Cao, Lefu Lan
Summary: This study investigated the mechanisms of action of BfmR in Pseudomonas aeruginosa, identifying 172 potential target genes and revealing connections between BfmR and the CzcRS and PhoBR two-component systems. Additionally, the results demonstrated the important role of phoB in BfmR-mediated promotion of biofilm formation.
Article
Multidisciplinary Sciences
Jeungeun Park, Yongsam Kim, Wanho Lee, Sookkyung Lim
Summary: This study presents a mathematical model to investigate the hydrodynamic interaction and swimming mechanism of lophotrichous bacteria. The results show that the swimming modes of the bacteria depend on the bending modulus of the hook and the magnitude of applied torques on the motor. The study also reveals the reoriented directions of the cells in three dimensions and the importance of transitions between different swimming modes.
SCIENTIFIC REPORTS
(2022)