Review
Food Science & Technology
Erica Tirloni, Simone Stella, Francesco Celandroni, Diletta Mazzantini, Cristian Bernardi, Emilia Ghelardi
Summary: Spore-forming Bacillus cereus is a common contaminant of dairy products that can contaminate milk at various stages of production. Pasteurization treatment is not effective in reducing contamination and may actually activate spore germination. The prevalence and concentration of B. cereus in milk and dairy products differ greatly worldwide. The production of biofilms by B. cereus is a major concern for the dairy industry, as it makes cleaning and transfer of spores difficult. The correct application of hygienic procedures is crucial to prevent and manage the circulation of B. cereus in the dairy supply chain.
Article
Food Science & Technology
Angela Maria Catania, Tiziana Civera, Pierluigi Aldo Di Ciccio, Maria Ausilia Grassi, Patrizia Morra, Alessandra Dalmasso
Summary: Processed cheeses from an Italian dairy plant were found to contain Bacillus strains in the initial production hours. Genetic analysis revealed different sequence types for Bacillus cereus strains and identification of a new sequence type for Bacillus subtilis strains. All isolates showed biofilm-forming ability, suggesting the presence of a recurring Bacillus population.
Article
Microbiology
Nay El-Khoury, Imene Bennaceur, Emilie Verplaetse, Stephane Aymerich, Didier Lereclus, Mireille Kallassy, Michel Gohar
Summary: During biofilm growth, dynamic exchanges occur between planktonic and sessile cells. The planktonic population grows until the biofilm forms, then rapidly decreases to zero, while recruited planktonic cells are found in specific areas with low sessile cell density within the biofilm, indicating spatial heterogeneity.
Article
Microbiology
Yangfu Li, Nuo Chen, Qingping Wu, Xinmin Liang, Xiaoming Yuan, Zhenjun Zhu, Yin Zheng, Shubo Yu, Moutong Chen, Jumei Zhang, Juan Wang, Yu Ding
Summary: This study found that knocking out the flagellar hook encoding gene flgE in emetic Bacillus cereus strains resulted in the loss of flagellar structure and swimming ability, defects in both pellicle and ring formation, and a decrease in cereulide synthesis. This suggests that flagellar components play an important role in biofilm formation and cereulide production in emetic B. cereus.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Yuxuan Qin, Leticia Lima Angelini, Yunrong Chai
Summary: This minireview discusses cell differentiation in Bacillus subtilis, a soil-dwelling, spore-forming Gram-positive bacterium, and its role in biofilm formation and prevalence in the environment. It provides a comprehensive review of the classic model of endospore formation in B. subtilis, as well as recent investigations on cell fate determination and generation of multiple cell types during biofilm formation. Mechanistic details of how cell fate determination and mutually exclusive cell differentiation are regulated during biofilm formation are also presented.
Article
Food Science & Technology
Jianwei Zhou, Yuhao Wu, Donghong Liu, Ruiling Lv
Summary: This study focuses on the biofilm characteristics of Bacillus licheniformis and Bacillus cereus in milk powder production. It investigates the effects of different carbon sources and temperatures on biofilm formation and metabolic activity. The results show that biofilm formation is enhanced with higher concentration of carbon source, particularly glucose and maltose. Skimmed milk improves the biomass of both Bacillus licheniformis and Bacillus cereus biofilms and the metabolic activity of Bacillus licheniformis biofilm. Lactose only improves the metabolic activity of Bacillus licheniformis biofilm, indicating it is not the main component affecting biofilm formation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Vanessa Pereira Perez Alonso, Jessica de Oliveira Morais, Dirce Yorika Kabuki
Summary: The study investigated the diversity of spore-forming bacilli in UHT milk, finding that B. cereus s.s., B. sporothermodurans, and G. stearothermophilus were commonly present in UHT milk and capable of forming biofilms. The data highlights the need for the dairy industry to strengthen control over raw materials and cleaning procedures to prevent the formation of persistent microbial communities.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yugenraj Navaneethan, Mohd Esah Effarizah
Summary: The study revealed the presence of Bacillus cereus in 100 ready-to-eat cooked rice samples from food outlets in Penang Island, with strains carrying multiple toxin genes and showing antibiotic resistance, including multidrug-resistant strains. These strains were also capable of forming biofilms in food, which could contribute to recurrent contamination.
Article
Biotechnology & Applied Microbiology
Linlin Zhao, Qing Liu, Qiubin Huang, Fengying Liu, Huiping Liu, Gang Wang
Summary: The study found that the isocitrate dehydrogenase of B. cereus is involved in biofilm formation and may regulate it by modulating redox homeostasis. Deletion of icdH may result in reduced biofilm yield, acidification of the extracellular matrix, and decreased sporulation rate.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Kyung-Min Park, Hyun-Jung Kim, Kee-Jai Park, Minseon Koo
Summary: This study investigated the virulence potential of mesophilic and psychrotolerant Bacillus cereus isolated from chilled tofu, including prevalence, toxin gene profile, antibiogram, and biofilm formation. B. cereus carrying enterotoxin genes were more frequently found in psychrotolerant isolates. Hemolysin BL and nonhemolytic enterotoxin complexes were found at a higher frequency in psychrotolerant isolates. Some B. cereus isolates showed resistance to rifampicin or clindamycin. Mesophilic isolates displayed the strongest biofilm formation at 40 and 42 degrees C, while the biofilm formation of psychrotolerant isolates was not significantly affected by temperature.
Article
Plant Sciences
Nan Yin, Jian-Long Zhao, Rui Liu, Yan Li, Jian Ling, Yu-Hong Yang, Bing-Yan Xie, Zhen-Chuan Mao
Summary: The study demonstrated that Bacillus cereus strain Bc-cm103 isolated from the rhizoplane of Cucumis metuliferus showed strong suppressive effect against Meloidogyne incognita, causing 100% mortality of nematode second-stage juveniles within 12 hours and reducing egg hatching rate by 40.06% within 72 hours compared to the control. Bc-cm103 formed a protective biofilm on cucumber roots, activated defense-responsive genes in cucumber, and significantly reduced root galls in pot, split-root, and field tests, indicating its potential as a biocontrol agent against this pathogen.
Article
Chemistry, Applied
XiaoMeng Wang, XueLiang Zhang, YuKe Wang, NanYu Tang, LuYao Xiao, JuanJuan Tian, Xin Rui, Wei Li
Summary: This study investigated the interaction among Kluyveromyces marxianus G-Y4, Lacticaseibacillus paracasei GL1, and Lactobacillus helveticus SNA12 isolated from Tibetan kefir grains. The results showed that G-Y4 promoted the growth of GL1 and SNA12 and improved their biofilm-forming ability. The cell wall polysaccharides produced by G-Y4 were found to be key substances that promote biofilm formation.
CARBOHYDRATE POLYMERS
(2023)
Article
Food Science & Technology
Lucas Donizete Silva, Maristela Mendes Aguiar, Aline Dias Paiva, Patricia Campos Bernardes, Rubens Gedraite, Emiliane Andrade Araujo Naves
Summary: This study aimed to evaluate the adhesion and biofilm formation of Bacillus cereus, investigate the efficiency of the conventional CIP process applied to dairy products, and propose an alternative form of the process with the pulsed flow in sanitization. The results showed that optimizing the CIP process can significantly increase the decimal reduction of Bacillus cereus and reduce downtime, energy consumption, and sanitizing agent usage.
Article
Food Science & Technology
Yanmei Li, Meng Wang, Yuqi Li, Bin Hong, Duo Kang, Yi Ma, Jufang Wang
Summary: Bacillus cereus is a widespread bacterium that causes food-associated gastrointestinal diseases. The excessive use of antibiotics and preservatives has led to the emergence of resistant B. cereus strains, impacting food safety. In this study, peptide derivatives based on the peptide D51 were designed and synthesized. Two of these peptides, D51-P11G and D51-P11K, showed promising antimicrobial activities against B. cereus, including reference strains. These peptides also demonstrated stability, safety, and low potential for inducing resistance. Additionally, D51-P11G and D51-P11K effectively inhibited spore germination and biofilm formation, making them ideal candidates for combating B. cereus.
Article
Food Science & Technology
Shuo Yang, Yating Wang, Fanchong Ren, Zhuosi Li, Qingli Dong
Summary: Bacillus cereus is a common causative agent of food poisoning outbreaks and can produce biofilms, causing problems in the food industry. Enzymes have been used as an alternative approach to destroy biofilms and have been effective in the removal of B. cereus biofilms. However, more research is needed on the specific application of enzymatic biofilm removal in different strains of B. cereus.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Nay El-Khoury, Imene Bennaceur, Emilie Verplaetse, Stephane Aymerich, Didier Lereclus, Mireille Kallassy, Michel Gohar
Summary: During biofilm growth, dynamic exchanges occur between planktonic and sessile cells. The planktonic population grows until the biofilm forms, then rapidly decreases to zero, while recruited planktonic cells are found in specific areas with low sessile cell density within the biofilm, indicating spatial heterogeneity.
Article
Chemistry, Applied
Adeline Sivignon, Shin-Yi Yu, Nathalie Ballet, Pascal Vandekerckove, Nicolas Barnich, Yann Guerardel
Summary: The study showed that polysaccharides such as mannan, S-glucan, and alpha-glucan found in yeast cell walls can strongly inhibit AIEC adhesion in vitro and accelerate AIEC elimination in vivo. These components could potentially be used as a treatment to eliminate AIEC bacteria in patients with Crohn's disease.
CARBOHYDRATE POLYMERS
(2021)
Review
Microbiology
Heloise Coullon, Thomas Candela
Summary: The cortex and peptidoglycan of Clostridioides difficile have been relatively understudied, but have gained increased interest in recent years due to their highly modified structures. These modifications may play a role in antimicrobial resistance and are crucial for spore cortex synthesis and germination, which are essential in the pathogenesis of the bacterium. Enzymes responsible for these modifications could be potential new drug targets or anti-C. difficile agents.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huan-Chuan Tseng, Cheng-Te Hsiao, Nao Yamakawa, Yann Guerardel, Kay-Hooi Khoo
Summary: Mass spectrometry-based high-sensitivity mapping of terminal glycotopes, particularly in adult zebrafish, has identified unique organ-specific N- and O-glycans. Through direct nano-LC-MS/MS analysis, the distribution and relative abundance of glycotopes and sialic acid variants have been established, revealing a full range of sulfated glycotopes in adult zebrafish, including unique ones in the brain. These findings expand the adult zebrafish glycome atlas and provide insight into sulfotransferases and glycosyltransferases, impacting neurobiology studies using zebrafish as a vertebrate model.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Di Wu, Pierre-Andre Gilormini, Sakura Toda, Christophe Biot, Cedric Lion, Yann Guerardel, Chihiro Sato, Ken Kitajima
Summary: This study investigated the role of the C-domain in the CMP-Kdn-dependent inhibition in rainbow trout and mice. The results showed that the C-domain affects the catalytic activity of rtCSS when using Kdn as a substrate, and the inhibition is C-domain-dependent.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Alexandre Mery, Samir Jawhara, Nadine Francois, Marjorie Cornu, Julien Poissy, Maria Martinez-Esparza, Daniel Poulain, Boualem Sendid, Yann Guerardel
Summary: Early diagnosis of invasive candidiasis (IC) is crucial for patient survival. The current gold standard diagnostic test has limitations such as low positivity rate and delayed results. However, mass spectrometry-based detection of circulating glycans provides a reliable and cost-effective early diagnostic method. Furthermore, high resolution mass spectrometry improves the performance of IC diagnosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Shi Yan, Adnan Hodzic, Giulia Bandini, Ganglong Yang, Jorick Vanbeselaere, Yann Guerardel
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jasmine Nour, Annalisa Moregola, Monika Svecla, Lorenzo Da Dalt, Rossella Bellini, Olivier Neyrolles, Gian Paolo Fadini, Yoann Rombouts, Mattia Albiero, Fabrizia Bonacina, Giuseppe Danilo Norata
Summary: Research has shown that during a high-fat diet, deficiency of the mannose receptor affects bone marrow and circulating immune cell subsets, leading to reduced systemic inflammation and resistance to obesity development.
Article
Biology
Nikos Nikolopoulos, Renata C. Matos, Stephanie Ravaud, Pascal Courtin, Houssam Akherraz, Simon Palussiere, Virginie Gueguen-Chaignon, Marie Salomon-Mallet, Alain Guillot, Yann Guerardel, Marie-Pierre Chapot-Chartier, Christophe Grangeasse, Francois Leulier
Summary: This study investigates the role of an uncharacterized protein (DltE) encoded by the dlt operon in Lactiplantibacillus plantarum. It is found that DltE acts as a D-Ala carboxyesterase, removing D-Ala from lipoteichoic acids (LTAs). The D-Ala-LTAs are shown to be important cues supporting intestinal peptidase expression and juvenile growth in Drosophila melanogaster.
Article
Microbiology
Jeanne Malet-Villemagne, Yucheng Liang, Laurent Evanno, Sandrine Denis-Quanquin, Jean-Emmanuel Hugonnet, Michel Arthur, Claire Janoir, Thomas Candela
Summary: Cell wall glycopolymers (CWGPs) in Gram-positive bacteria have been found to play important roles in various bacterial processes, including surface setup and virulence. The anchoring of CWGPs to peptidoglycan is crucial for bacterial growth and survival. Through studying a specific clinical strain, it was discovered that the competition between CWGPs and peptidoglycan synthesis is essential for bacterial survival.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Laure Papin, Martin Lehmann, Justine Lagisquet, Ghizlane Maarifi, Veronique Robert-Hebmann, Christophe Mariller, Yann Guerardel, Lucile Espert, Volker Haucke, Fabien P. Blanchet
Summary: DC-SIGN is a critical receptor involved in host immunity and autophagy pathway, promoting autophagy flux and enhancing the body's antiviral immune response. It is associated with early endocytic events and plays a role in virus resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Pierre-Alexandre Lacotte, Sandrine Denis-Quanquin, Eva Chatonnat, Julie Le Bris, David Leparfait, Thierry Lequeux, Isabelle Martin-Verstraete, Thomas Candela
Summary: The dlt operon encodes proteins responsible for modifying the structure of bacteria, altering the physicochemical properties of the cell surface and reducing sensitivity to antibiotics.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Audrey Hamiot, Christelle Lemy, Frederic Krzewinski, Christine Faille, Thomas Dubois
Summary: Spore-forming bacteria of the Bacillus subtilis group are responsible for recurrent contamination of processing lines in the food industry. The study found that sporulation conditions affect the surface and adhesion properties of B. subtilis spores.
FRONTIERS IN MICROBIOLOGY
(2023)
Letter
Biochemistry & Molecular Biology
Masae Hosoda, Kazuhiro Aoki, Yann Guerardel, Issaku Yamada, Kiyoko F. Aoki-Kinoshita
Article
Biochemistry & Molecular Biology
Wassim Daher, Louis-David Leclercq, Matt D. Johansen, Claire Hamela, Jona Karam, Xavier Trivelli, Jerome Nigou, Yann Guerardel, Laurent Kremer
Summary: Mycobacterium abscessus is a difficult-to-manage mycobacterial species that can exhibit different morphotypes and glycosylation patterns. This study reveals the importance of glycopeptidolipids in internalization, morphotype transition, virulence, and infection outcomes of M. abscessus.
CELL CHEMICAL BIOLOGY
(2022)
